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See detailLe berceau d'une collection : les incunables de Charles Van Hulthem (1764-1832)
Adam, Renaud ULg

in In Monte Artium (2011), 4

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See detailTropfenpopulationsbilanzen zur Auslegung von Extraktionskolonnen
Adinata, Donni; Ayesteran, José M.; Buchbender, Florian et al

in Chemie Ingenieur Technik (2011), 83(7), 952964

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See detailLes Codes La Charte. Droit international conventionnel et Droit administratif
Bourgeois, Marc ULg; Behrendt, Christian ULg; Lewalle, Paul ULg et al

Book published by La Charte (2011)

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See detailReciprocal interactions between wakefulness and sleep influence global and regional brain activity
Muto, Vincenzo ULg; Mascetti, Laura ULg; Matarazzo, Luca et al

in Current Topics in Medicinal Chemistry (2011), 11(19), 2403-13

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See detailAltered secretion of pregnancy-associated glycoproteins during gestation in bovine somatic clones.
Constant, Fabienne; Camous, Sylvaine; Chavatte-Palmer, Pascale et al

in Theriogenology (2011), 76(6), 1006-1021

Somatic nuclear transfer (NT) in cattle is often accompanied by severe placental anomalies, hypertrophy, and hydrallantois, which induce a high rate of pregnancy losses throughout gestation. These ... [more ▼]

Somatic nuclear transfer (NT) in cattle is often accompanied by severe placental anomalies, hypertrophy, and hydrallantois, which induce a high rate of pregnancy losses throughout gestation. These placental deficits are associated with an abnormal increase of the maternal plasma levels of pregnancy-associated glycoprotein (PAG), produced by the trophoblastic binucleate cells (BNC) of the placenta. The objective of this study was to analyze the origin of the abnormally elevated PAG concentrations in the peripheral circulation of NT recipients during pathological pregnancies. Concentrations of PAG were measured both in maternal blood, in chorionic and cotyledonary tissular extracts from control recipients (after artificial insemination, AI, or in vitro fertilization, IVF) and clone recipients on Day 32, Day 62, and during the third trimester of gestation. Three different radioimmunoassay (RIA) systems were used. One homologous RIA for PSP60, similar to bovine PAG-1 (PAG(67kDa)), and two heterologous RIA with PAG(67kDa) as standard and tracer, and antisera anti-caprine PAG (AS#706 and AS#708). Circulating and tissular concentrations of bovine placental lactogen (bPL), a glycoprotein also produced by BNC, were determined by RIA at the same stages. The number of BNC in the placental tissues was determined by cell counting after immunostaining with anti PSP60 antibody on tissue sections from control and NT pregnancies. Maternal plasma PAG concentrations were not different among groups on Day 32, but they were significantly higher in NT than in control pregnancies on Day 62 with all three RIA and during the third trimester with two RIA (RIA-PSP60 and RIA with AS#708). Circulating bPL concentrations were undetectable on Days 32 and 62 and were not different in the third trimester between NT and control pregnancies. Tissular amounts of PAG on total proteins were not different between the two groups at all stages studied. No difference was determined in the percentage of PSP60-positive BNC in placental tissues between controls and NT on Day 62 and during the third trimester of pregnancy. Western blots of tissular extracts from placenta showed no major molecular weight changes of PAG in NT pregnancies compared to controls. No differences in maternal circulation concentrations or tissular content of bPL were observed between control and NT pregnancies. In conclusion, the specific increase of PAG in maternal plasma concentrations during abnormal NT pregnancies do not result from a higher proportion of BNC, or an increased protein expression of PAG and could be due to changes in the composition of terminal glycosylation which result into a clearance decrease of PAG from the circulation. [less ▲]

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See detailDO PLACEBO BASED VALIDATION STANDARDS MIMIC REAL BATCH PRODUCTS BEHAVIOUR? CASE STUDIES
Bouabidi, A.; Talbi, M.; Bouklouze, A. et al

in Journal of Pharmaceutical & Biomedical Analysis (2011), 55

Analytical methods validation is a mandatory step to evaluate the ability of developed methods to provide accurate results for their routine application. Validation usually involves validation standards ... [more ▼]

Analytical methods validation is a mandatory step to evaluate the ability of developed methods to provide accurate results for their routine application. Validation usually involves validation standards or quality control samples that are prepared in placebo or reconstituted matrix made of a mixture of all the ingredients composing the drug product except the active substance or the analyte under investigation. However, one of the main concerns that can be made with this approach is that it may lack an important source of variability that come from the manufacturing process. The question that remains at the end of the validation step is about the transferability of the quantitative performance from validation standards to real authentic drug product samples. In this work, this topic is investigated through three case studies. Three analytical methods were validated using the commonly spiked placebo validation standards at several concentration levels as well as using samples coming from authentic batch samples (tablets and syrups). The results showed that, depending on the type of response function used as calibration curve, there were various degrees of differences in the results accuracy obtained with the two types of samples. Nonetheless the use of spiked placebo validation standards was showed to mimic relatively well the quantitative behaviour of the analytical methods with authentic batch samples. Adding these authentic batch samples into the validation design may help the analyst to select and confirm the most fit for purpose calibration curve and thus increase the accuracy and reliability of the results generated by the method in routine application. [less ▲]

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See detailMitotic spindle: focus on the function of huntingtin
Godin, Juliette ULg; Humbert, Sandrine

in International Journal of Biochemistry & Cell Biology (2011), 43(6), 852-856

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See detailLe frontispice du Livre d’heures de Jacques Lefèbvre et Jacqueline Heughe : entre dévotion et ostentation
Falque, Ingrid ULg

in PYCKE, Jacques (Ed.) Archives et manuscrits précieux tounaisiens, 4 (2011)

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See detailFlow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.
Wauters, Jella; Franck, Thierry ULg; Pille, Frederik et al

in Veterinary Immunology and Immunopathology (2011), 144(3-4), 417-22

Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However ... [more ▼]

Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively. [less ▲]

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See detailPotential mineral deficiencies for Ndama cattle grazing Urochloa sp. based tropical pastures in the Bas-Congo province of the Democratic Republic of Congo
Picron, Pascale ULg; Goubau, Amaury ULg; Lecomte, Thomas et al

in Advances in Animal Biosciences (2011), 2(2), 388

Artificial pastures are used to increase carrying capacity in the wet tropics by supplying higher quality forage (energy and protein) to the animals all year long. Sowing pastures is labour-intensive so ... [more ▼]

Artificial pastures are used to increase carrying capacity in the wet tropics by supplying higher quality forage (energy and protein) to the animals all year long. Sowing pastures is labour-intensive so to be profitable all other possible growth limiting factors, especially minerals, should be alleviated. We studied nutrients intake (energy, protein and minerals) in cattle grazing Urochloa sp. pastures in Western DRC. Three Urochloa ruziziensis and 3 U. decumbens pastures in Kolo-Fuma (Bas-Congo, DRC) were studied. Three Ndama steers and 3 cows grazing each pasture consecutively during the short rainy and dry seasons were followed by handplucking and samples were analysed for energy, crude protein and ash to calculate energy value (fodder units, FU) and digestible crude protein content (DCP) of the diets. NIRS was used on faeces to determine dry matter (DM) intake and ICP-AES to determine mineral content of the diets. Intake levels reached 66 ± 4.3 g kg-1LW0.75, nutritive value of forage was 0.701 ± 0.036 FU and 4.78 ± 1.04 % DCP, allowing daily weight gains > 550 g for steers and > 350 g for cows. P, Ca, Mg, K, Mn & Fe were provided above requirements by the pasture. Na, Cu and Zn were deficient, especially during the short dry season for Cu and Zn. U. ruziziensis pastures tended to provide more minerals, especially during the rainy season. A mineral supplement providing Na, Cu and Zn is required to reach the daily weight gains allowed by energy and protein supplies. The supplement could reasonably be similar for U. decumbens and U. ruziziensis pastures, but the dry season formula should provide more Cu and Zn than the rainy season formula [less ▲]

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See detailFirst retrievals of methyl chloride from ground-based high-resolution FTIR solar observations
Mahieu, Emmanuel ULg; Harrison, Jeremy; Bernath, Peter F. et al

in Geophysical Research Abstracts (2011), 13

Methyl chloride (CH3Cl) is one of the most abundant chlorine-bearing gas in the Earth’s troposphere and a significant contributor to the organic chlorine budget. Measurements by in situ networks indicate ... [more ▼]

Methyl chloride (CH3Cl) is one of the most abundant chlorine-bearing gas in the Earth’s troposphere and a significant contributor to the organic chlorine budget. Measurements by in situ networks indicate a mean volume mixing ratio of 550 pptv, with a significant seasonal cycle of about 80 pptv, peak to peak. This species also exhibits inter-annual variability, but no long-term trend. Major sources are from tropical and sub-tropical plants and dead leaves, the oceans and biomass burning. Some industrial processes and waste incineration further add to the emissions. Oxidation by the hydroxyl radical is by far the largest CH3Cl sink, followed by soil uptake. Although balanced, its atmospheric budget is still affected by large uncertainties and contributions from unidentified sources and sinks cannot be ruled out. Methyl chloride has an atmospheric lifetime of 1 year, a global warming potential of 13 (100-yr horizon) and an ozone depleting potential of 0.02. The retrieval of methyl chloride from ground-based infrared (IR) spectra is very challenging. Indeed, numerous interferences by strong water vapor and methane lines complicate the detection of small CH3Cl absorptions, close to 1%, near 3 microns. In addition, and although weak, ethane features contribute to the difficulty, in particular since a significant number of ethane branches were absent until very recently from official HITRAN compilations. Therefore, the scientific literature does not report thus far about any investigations of CH3Cl from ground-based remote sensing observations. In this contribution, we will present first CH3Cl total column retrievals, using the SFIT-2 algorithm (v3.94) and high-resolution Fourier Transform Infrared (FTIR) solar absorption observations recorded with a Bruker 120HR instrument, at the high altitude station of the Jungfraujoch (46.5°N, 8°E, 3580 m asl), within the framework of the Network for the Detection of Atmospheric Composition Change (NDACC, visit http://www.ndacc.org). In our retrievals, we use new ethane absorption cross sections recorded at the Molecular Spectroscopy Facility of the Rutherford Appleton Laboratory (Harrison et al., 2010). They were calibrated in intensity by using reference low-resolution spectra from the Pacific Northwest National Laboratory (PNNL) IR database. These new cross sections were recently released as a HITRAN update (see http://www.hitran.com). Pseudoline parameters fitted to these ethane spectra have been combined with HITRAN 2004 line parameters (including all the 2006 updates) for all other species encompassed in the selected microwindows, including our target CH3Cl. We will evaluate the improvement brought by the new ethane line parameters on the fitting residuals, and characterize the quality, the precision and the reliability of the retrieved product. If successful, a long-term CH3Cl total column time series will be produced using the Jungfraujoch observational database, and we will perform preliminary investigations of the seasonal and inter-annual variations of methyl chloride total columns at northern mid-latitudes. [less ▲]

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See detailScreening for colorectal cancer in asymptomatic average risk patients: role of imaging.
Hock, D.; Ouhadi, R.; Materne, R. et al

in Acta Gastro-Enterologica Belgica (2011), 74(1), 70-6

Early detection of colorectal cancer or advanced adenomas is a public health priority in many industrialized countries. There are various methods of screening average risk individuals for colorectal ... [more ▼]

Early detection of colorectal cancer or advanced adenomas is a public health priority in many industrialized countries. There are various methods of screening average risk individuals for colorectal cancer, and their effectiveness may depend on subjective parameters like local expertise and patient's preferences. This paper reviews these tests with special emphasis regarding imaging techniques that aim to provide less-invasive alternatives to optical colonoscopy (OC) which is the standard of reference. Both Double-Contrast Barium Enema (DCBE) and Virtual Colonoscopy (VC) have >90% sensitivity compared to OC in the detection of clinically relevant colonic lesions. Nevertheless, VC may have an edge over DCBE for technical and reproductivity reasons, as well as greater learning opportunities. Imaging techniques criticisms regarding diminutive and flat lesions, cost, radiation exposure and effects on gastroenterological practice are addressed. [less ▲]

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See detailNonlinear Normal Modes: A Finite Element Approach
Renson, Ludovic ULg; Kerschen, Gaëtan ULg

Conference (2011)

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See detailUsing putative anti-auxins to improve somatic embryo maturation of Abies nordmanniana
Tahiri, Abdelghani ULg; Misson, Jean-Pierre; Kevers, Claire et al

Poster (2011)

In Belgium, the culture of Nordmann fir (Abies nordmanniana) is mainly located in Wallonia region. The planted area is estimated in average at 5000 ha  that annualy produce some 4 million Christmas trees ... [more ▼]

In Belgium, the culture of Nordmann fir (Abies nordmanniana) is mainly located in Wallonia region. The planted area is estimated in average at 5000 ha  that annualy produce some 4 million Christmas trees destined in priority to the export market. In vitro propagation by somatic embryogenesis is considered as an alternative method to seedlings for producing homogeneous and orthotropic trees on a large scale. Currently, the performances of the somatic embryo (SE) maturation is still insufficient to consider any commercial application of the technology. In this way , the effect of three substances  (2,3,5-triiodobenzoïque acid  as TIBA, 2 - (p-chlorophenoxy)-2-methylpropionic acids as PCIB, p-hydroxbenzoïque as PHBA)  reported as ''anti-auxins'' have been tested with three embryogenic lines on the formation and maturation of normal somatic embryos. [less ▲]

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See detailPhysico-chemical mechanisms affecting the adherence of starch granules on materials with different hydrophobicities
Detry, Jean; Sindic, Marianne ULg; Servais, Marjorie et al

in Journal of Colloid & Interface Science (2011), 355

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See detailDeterminants of high, median and low rates of caesarean deliveries in Belgium
Absil, Gaëtan ULg; Van Parys, A. S.; Bednarek, Stéphanie et al

Report (2011)

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See detailExperimental procedure and statistical data treatment for the kinetic study of selective hydrodechlorination of 1,2-dichloroethane into ethylene over a Pd-Ag sol–gel catalyst
Pirard, Sophie ULg; Pirard, Jean-Paul ULg; Heyen, Georges ULg et al

in Chemical Engineering Journal (2011), 173(3), 801-812

The kinetics of selective hydrodechlorination of 1,2-dichloroethane into ethylene over a Pd- Ag/SiO2 catalyst was studied using an a priori experimental design with five independent variables—temperature ... [more ▼]

The kinetics of selective hydrodechlorination of 1,2-dichloroethane into ethylene over a Pd- Ag/SiO2 catalyst was studied using an a priori experimental design with five independent variables—temperature and partial pressures of 1,2-dichloroethane, hydrogen, ethylene and hydrogen chloride. A Langmuir–Hinshelwood model including two types of active site and the 1,2-dichloroethane adsorption as the rate-determining step was found to fit correctly with experimental data, according to the analysis of variance and the analysis of pondered residuals. The study allowed for catalytic deactivation. The rigorous experimental and statistical approach followed to carry out such a kinetic study is explained in detail. [less ▲]

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See detailSubclinical diseases underlying poor performance in endurance horses: diagnostic methods and predictive tests.
Fraipont, Audrey ULg; Van Erck, E.; Ramery, Eve ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011), 169(6), 154

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler ... [more ▼]

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler echocardiography, impulse oscillometry, video endoscopy and collection of respiratory fluids. All of the examined poorly performing horses were affected by subclinical diseases, and most of them had multiple concomitant disorders. On the contrary, the well-performing horses were free of any subclinical disease. The most frequently diagnosed diseases were respiratory disorders, followed by musculoskeletal and cardiac problems. Poor performers exhibited lower speeds at blood lactate concentration of 4 mmol/l (VLA4) and at heart rates of 160 (V160) and 200 bpm (V200) on the treadmill and in the field, as well as slower recovery of heart rate. [less ▲]

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