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See detailEvaluation of organic dust components cytotoxicity on thp1 monocytes-derived-macrophages using high content analysis
Ramery, Eve ULg; O'Brien, Peter James

in Veterinary Clinical Pathology (2011, December), 40(4), 583

Background: Organic dust contains pathogen associated molecular patterns (PAMPs) which can induce, following chronic exposure, significant airway diseases. Mononuclear phagocytes are key protecting cells ... [more ▼]

Background: Organic dust contains pathogen associated molecular patterns (PAMPs) which can induce, following chronic exposure, significant airway diseases. Mononuclear phagocytes are key protecting cells of the respiratory tract. Several studies have investigated the effects of PAMPs, and mainly endotoxins, on cytokine production. However the sub-lethal cytotoxicity of organic dust components on macrophages has not been tested yet. The novel technology of high content analysis (HCA) is already used to assess subclinical drug-induced toxicity. It combines the capabilities of flow cytometry, intracellular fluorescence probes, and image analysis and enables to perform rapid multiple analysis in large numbers of samples. Objectives: The purpose of the present study was, by using HCA, to investigate the cytotoxicity of the 3 major PAMPs contained in organic dust, ie. endotoxin (LPS), peptidoglycan (PGN) and β-glucans (zymosan) on THP-1 monocyte-derived macrophages. Methods: LPS was used at concentrations of 0.005, 0.01, 0.02, 0.05, 0.1 and 1 μg/ml; PGN and zymosan were used at concentrations of 1, 5, 10, 50, 100 and 500 μg/ml. Cells were exposed to PAMPs for 24 hours. In addition, the oxidative burst and the phagocytic capabilities of the cells were tested. Results: An overlap between PGN intrinsic fluorescence and red/far-red fluorescent dyes occurred, rendering the evaluation of some parameters impossible for PGN. LPS induced sub-lethal cytotoxicity at the lowest dose (from 10 ng/ml). However, the most spectacular changes occurred with zymosan. In addition, zymosan, but not LPS, induced phagosome maturation and oxidative burst. Conclusions: Given the fact that β-glucans can be up to 100 fold more concentrated in organic dust than LPS, these results suggest that β-glucans could play a major role in macrophages impairment following heavy dust exposure and will deserve further investigation in the near future. [less ▲]

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See detailNovel amphiphilic mikto-arm star-shaped copolymers for the preparation of PLA-based nanocarriers
Cajot, Sébastien ULg; Riva, Raphaël ULg; Billiet, Leen et al

in Macromolecular Symposia (2011), 309/310(1), 111-122

Three-arm (A2B) and four-arm (A2B2) star-shaped copolymers based on biocompatible and biodegradable hydrophobic poly(ε-caprolactone) (PCL) (A arms) and biocompatible and bioeliminable hydrophilic poly ... [more ▼]

Three-arm (A2B) and four-arm (A2B2) star-shaped copolymers based on biocompatible and biodegradable hydrophobic poly(ε-caprolactone) (PCL) (A arms) and biocompatible and bioeliminable hydrophilic poly(ethylene oxide) (PEO) (B arms) were synthesized by the coupling of an ω-azide terminated PEO chains with PCL chain bearing one (A2B) or two (A2B2) alkyne functions at the middle of the chain by the copper mediated azide-alkyne cycloaddition (CuAAC). The amphiphilic behavior of these different stars was confirmed by micellization experiments in water followed by dynamic light scattering and transmission electron microscopy analyses. The efficiency to stabilize PLA nanoparticles was investigated in function of the stars structure. [less ▲]

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See detailLes cultes isiaques en Grèce. Introduction. Actes du IVe colloque international sur les études isiaques, Troisième journée, Université de Liège, 29 novembre 2008, Michel Malaise in honorem
Veymiers, Richard ULg; Bricault, Laurent

in Veymiers, Richard; Bricault, Laurent (Eds.) Bibliotheca Isiaca II (2011)

Introduction thématique à la publication des Actes de la troisième journée du IVe colloque international sur les études isiaques, Université de Liège, 29 novembre 2011, Michel Malaise in honorem.

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See detailEntretien avec David Toscana
Vanden Berghe, Kristine ULg

in Caravelle : Cahiers du Monde Hispanique et Luso-Bresilien (2011), 97(2), 241-248

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See detailLayering of Size and Type Noun Constructions in English
Brems, Lieselotte ULg

Book published by Mouton de Gruyter - 1st (2011)

On the basis of synchronic and diachronic data analysis, the volume takes a close look at the synchronic layers of binominal size noun and type noun uses (a bunch/a load of X; a sort of X; a Y type of X ... [more ▼]

On the basis of synchronic and diachronic data analysis, the volume takes a close look at the synchronic layers of binominal size noun and type noun uses (a bunch/a load of X; a sort of X; a Y type of X) and reconsiders the framework of grammaticalization in view of issues raised by the phrases under discussion. As a result, a construction grammar-approach to grammaticalization is developed which does justice to the syntagmatic lexical, or collocational, reclustering observed in the data within an eclectic cognitive-functional approach. Prices subject to change. Shipping costs will be added. Current pricing is based [less ▲]

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See detailRapport d'activité du projet Health CoP 2011
Denis, Brigitte ULg; Snoeck, Céline ULg

Report (2011)

Rapport d'activité en lien avec les actions menées en milieu hospitalier. Création et animation de communautés de pratique supportées par les TIC.

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See detailPréface [à "Blancs et Noirs"]
Dejasse, Erwin ULg

in Blancs et Noirs (2011)

Foreword of a portfolio devoted to the jazz by Argentinian artist José Muñoz

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See detailLotze en Amérique. Le renouveau réaliste en philosophie chez Santayana
Boccaccini, Federico ULg

Conference (2011, December)

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See detailIs hepatitis E virus zoonotic?
Thiry, Damien ULg

Conference (2011, December)

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See detailDroit international privé
Pfeiff, Silvia ULg

in Droit des personnes et des familles : chronique de jurisprudence 2005-2010 (2011)

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See detailEN1198-5 training : computation examples
Cerfontaine, Benjamin ULg

Conference given outside the academic context (2011)

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See detailNightglow investigation in the Visible Range with the VIRTIS/Venus-Express instrument
Migliorini, A.; Slanger, T.; Saran, D. et al

Poster (2011, December)

In this study we report the investigation of the nightglow molecular oxygen emissions of Venus in the visible spectral range, observed by the VIRTIS instrument (Visible and InfraRed Spectral Imaging ... [more ▼]

In this study we report the investigation of the nightglow molecular oxygen emissions of Venus in the visible spectral range, observed by the VIRTIS instrument (Visible and InfraRed Spectral Imaging Spectrometer) on board the Venus Express spacecraft. The Herzberg II system was detected in the range 0.4-0.7 µm. The observed bands peak at 95-96 km altitude, with a limb mode intensity of 15-20 kR for the strongest band, similar to that seen in previous observations. Simultaneous observations in the visible and IR [O2 (a-X) 1.27 µm band] were obtained in March 2007, and the two systems are similar in peak altitude. In addition, three bands of the O2 Chamberlain system, at 0.558, 0.604, and 0.657 µm were detected. They are well detached from the Herzberg II bands, and more distinct than reported in previous observations (Garcia Munoz et al., 2009). A simulated spectrum is obtained using the DIATOM code, allowing accurate reproduction of the observed VIRTIS mean spectrum. [less ▲]

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See detailMorphological and histological studies of sheep’s brain
Salouci, Moustafa ULg; Engelen, Virginie; Jacqmot, Olivier ULg et al

Poster (2011, December)

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the ... [more ▼]

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and cerebellum cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus. [less ▲]

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See detailArchéologie et géomatique
Billen, Roland ULg

Article for general public (2011)

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See detailGolden-headed lion tamarin research in the 21st century: Recent advances and potential areas of future research
Raghunathan, Poornima ULg

in Neotropical Primates (2011), 18(2), 72-76

On 7 and 8 December 2011, students, researchers, and conservationists with a vested interest in golden-headed lion tamarins (Leontopithecus chrysomelas; GHLTs) gathered at the State University of Santa ... [more ▼]

On 7 and 8 December 2011, students, researchers, and conservationists with a vested interest in golden-headed lion tamarins (Leontopithecus chrysomelas; GHLTs) gathered at the State University of Santa Cruz (UESC; Ilhéus, Bahia, Brazil) for the symposium `Golden-Headed Lion Tamarin Research in the 21st Century: Recent Advances and Potential Areas of Future Research´ with the aim of sharing recent work and discussing potential future avenues for research. Within the last 5 years, several doctoral dissertations and masters theses were completed that focused on the biology, ecology, and/or conservation of GHLTs in addition to the ongoing work of established scientists who have devoted their professional lives to the study of this species and the Atlantic Forest. However, language barriers and the fact that many members of the GHLT community are based at institutions throughout the world have complicated widespread access to these results and collaborations among researchers. The primary goals of this symposium were to (1) promote the exchange of existing information, (2) contribute towards a better synchronization of research efforts, and (3) identify important steps for more efficient/ collaborative conservation efforts for GHLTs and their habitat. This symposium brought together 30 participants from 12 institutions in Brazil, Belgium and the USA and allowed for the dissemination of information to the global GHLT community, compilation of recent advances in research, and identification of gaps in knowledge of GHLT biology, ecology and conservation, which ultimately fostered discussions on how attendees could collaborate to fill knowledge gaps. [less ▲]

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See detailThe Future of Public Enterprises: Contributions to a new Discourse
Fecher-Bourgeois, Fabienne ULg; Florio, Massimo

in Annals of Public and Cooperative Economics = Annales de l'Economie Publique, Sociale et Coopérative (2011), 82(4), 361-516

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See detailAURALIAS: an audio-immersive system for auralizing room acoustics projects
Embrechts, Jean-Jacques ULg

Conference (2011, December)

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See detailLa place des Eglises de réveil dans les accusations de sorcellerie portées sur enfants en RDC
Demart, Sarah ULg

in Enfants accusés de sorcellerie (2011, December)

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