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See detailClostridium difficile in farm and slaughterhouse animals: prevalence and typing
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; van Broeck, Johan et al

Scientific conference (2011, December 09)

Clostridium difficile is an anaerobic gram-positive spore-forming bacillus identified as a severe pathogenic agent in animals and humans. Its recent isolation in foods opens up the occurrence of ... [more ▼]

Clostridium difficile is an anaerobic gram-positive spore-forming bacillus identified as a severe pathogenic agent in animals and humans. Its recent isolation in foods opens up the occurrence of alimentary origin infections. The objective of the study is to establish if there is a reservoir of bacteria in animals engaged to human feed and consequent true risk of transmission to humans through the food chain. A total of 437 fecal samples were analyzed. Stools (202 from beef and 194 from pigs) were collected at the slaughterhouse and from breeding farms (18 from calves and 23 from piglets). Enrichment culture was carried out using a cycloserine cefoxitin fructose taurocholate broth. Isolates were identified by PCR detection of tpi, tcdA, tcdB and cdtA genes. Toxic activity was confirmed by a fecal cytotoxin inmmunoessay. Further characterization was performed by PCR ribotyping. Clostridium difficile was cultured from 8% (35/437) of the total samples: 14 from beef at slaughterhouse (6,9%), 17 from piglets (73,9%) and 4 from calves (22,2%) at breeding farms. Thirty-two of the total strains were toxin positive. Sixteen different PCR ribotypes were identified with type 078 predominant in breeding farms of calves (75%) and pigs (64,7%). Isolates from beef presented widest range in ribotypical variety. Present study proves the presence of Clostridium difficile in animals engaged to food in Belgium. The presence of the bacteria at the slaughterhouse and the high prevalence of pathogenic strains states a true risk of contamination to humans through the food chain. [less ▲]

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See detailCaractérisation des viandes bovines à très longue durée de conservation sous vide
Didimo Imazaki, Pedro Henrique ULg; Nezer, Carine ULg; Taminiau, Bernard ULg et al

Poster (2011, December 09)

Le but de cette étude a été d’évaluer la conservabilité de viandes bovines de différentes origines (Royaume-Uni et Irlande, Australie et Brésil) et l’influence sur celle-ci de la température de ... [more ▼]

Le but de cette étude a été d’évaluer la conservabilité de viandes bovines de différentes origines (Royaume-Uni et Irlande, Australie et Brésil) et l’influence sur celle-ci de la température de conservation (1 °C vs. +4 °C). Des paramètres physico-chimiques (pH, couleur, proportion des différentes formes redox de la myoglobine (FRMb), indice TBARS et acides organiques) et microbiologiques (flore aérobie totale, flore lactique, Enterobacteriaceae, Pseudomonas spp. et Brochothrix thermosphacta) ont été mesurés sur sept lots de contre-filet conditionnés sous vide : aux ⅔ de la DLC et à la fin de la DLC. La diversité bactérienne a été évaluée par galeries API50 CHL et par métagénomique. Le pH a diminué au cours de la conservation dans deux lots. La couleur et la proportion des FRMb sont restées stables. Une augmentation de l’indice TBARS, plus prononcée à +4 °C, a été observée. Les viandes australiennes et brésilienne ont présenté des taux en acides acétique et citrique plus élevés. Tous les lots conservés à 1 °C ont présenté une qualité microbiologique satisfaisante à la fin de leur DLC (viandes britanniques et irlandaises = 35 ~ 45 jours; australiennes = 140 jours et brésilienne = 120 jours). La conservation à +4 °C a favorisé la croissance d’entérobactéries, facteur limitant de la conservation de plusieurs lots. L’identification bactérienne a révélé la présence de bactéries connues pour leur effet bioprotecteur. La phase ultérieure de ce travail consistera à étudier la dynamique de la flore microbienne endogène en fonction des conditions environnementales appliquées (température, atmosphère). [less ▲]

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See detailKnowledge and its value
Janvier, Antoine ULg; Leclercq, Bruno ULg

Conference (2011, December 09)

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See detailLong-term biogeochemical impacts of liming the ocean
Ilyina, Tatiana; Wolf-Gladrow, Dieter; Munhoven, Guy ULg et al

Conference (2011, December 08)

Fossil fuel CO2 emissions result in large-scale long-term perturbations in seawater chemistry. Oceans take up atmospheric CO2, and several geo-engineering approaches have been suggested to mitigate ... [more ▼]

Fossil fuel CO2 emissions result in large-scale long-term perturbations in seawater chemistry. Oceans take up atmospheric CO2, and several geo-engineering approaches have been suggested to mitigate impacts of CO2 emissions and resulting ocean acidification that are based on this property. One of them is to enhance weathering processes to remove atmospheric CO2. This method involves dissolving rocks (i.e. limestone) or adding strong bases (i.e. calcium hydroxide) in the upper ocean and is termed as liming the oceans. The net effect of this approach is to increase ocean alkalinity, thereby increasing the oceanic capacity to store anthropogenic CO2. Another effect of adding alkalinity would be to drive seawater to higher pH values and thus counteract the ongoing ocean acidification. However, whereas adding bases only alter alkalinity of seawater, dissolution of carbonates perturb both, alkalinity and dissolved inorganic carbon budgets. Thus, on longer time scales, these two methods will likely have different biogeochemical effects in the ocean. Here we test enduring implications of the two approaches for marine carbon cycle using the global ocean biogeochemical model HAMOCC. In our model scenarios we add alkalinity in the amounts proportional to fossil fuel emissions. We compare the longterm effectiveness of the two geo-engineering approaches to decrease atmospheric CO2. [less ▲]

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See detailEtude sur les pratiques de formation des "nouveaux entrants" au sein du secteur Agoria
Remy, Céline ULg

Conference (2011, December 08)

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See detailTable ronde autour de "La matrice de la race" (E. Dorlin)
Pieret, Denis ULg

Scientific conference (2011, December 08)

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See detailChitosan-based biomimetic scaffolds and methods for preparing the same
Filée, Patrice; Freichels, Astrid ULg; Jérôme, Christine ULg et al

Patent (2011)

The invention concerns chitosan biomimetic scaffolds and methods for modulating their intrinsic properties such as rigidity, elasticity, resistance to mechanical stress, porosity, biodegradation and ... [more ▼]

The invention concerns chitosan biomimetic scaffolds and methods for modulating their intrinsic properties such as rigidity, elasticity, resistance to mechanical stress, porosity, biodegradation and absorbance of exudates. Therefore, the present invention relates to a layered chitosan scaffold wherein said layered scaffold comprises at least two fused layers, wherein at least one of the fused layers comprises a chitosan nanofiber membrane and the other fused layer comprises a porous chitosan support layer. Moreover, the present invention provides a layered chitosan scaffold characterized by (i) a good adhesion between the porous and nanofiber layers, (ii) a tuneable porosity of the nanofiber layer by tuning the distance between the nanofibers, (iii) a stable nanofibers and porous morphology even when immersed in water or other solvents and a process for the preparation of such layered chitosan scaffold. Finally, the present invention provides the use of the layered electrospun chitosan scaffold of the invention or the layered electrospun chitosan scaffold produced by the process of the invention as a wound dressing, in tissue engineering or for biomedical applications. [less ▲]

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See detailThe secrets of the Kinect ... in depth!
Lejeune, Antoine ULg; Van Droogenbroeck, Marc ULg; Verly, Jacques ULg

Conference (2011, December 08)

The slides present the Kinect in depth. The major technological ideas are explained. In addition, the presentation gives an overview of all the libraries available to interface to the Kinect.

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See detailFormation à la microtomographie
Léonard, Angélique ULg; Toye, Dominique ULg; Plougonven, Erwan ULg

Learning material (2011)

This course is a two-day presentation of the basic workings of X-ray tomography, current local applications of microtomography in the applied chemistry department, typical image analysis tools for ... [more ▼]

This course is a two-day presentation of the basic workings of X-ray tomography, current local applications of microtomography in the applied chemistry department, typical image analysis tools for extracting measurements, and a practical look at a particular piece of equipment, the Skyscan 1172 desktop microtomograph, and its software. The target audience is users of such equipment, with little to no background in X-ray physics, computer science or image analysis. [less ▲]

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See detailEquivariant quantization in supergeometry
Radoux, Fabian ULg

Scientific conference (2011, December 08)

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See detailTactique et techniques du corps dans "L'An 5 de la révolution algérienne" de Frantz Fanon
Cormann, Grégory ULg

Scientific conference (2011, December 08)

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See detailAssessment of Mytilus galloprovincialis to monitor 19 trace elements in the Calvi Bay
Richir, Jonathan ULg; Vermeulen, Simon; Biondo, Renzo ULg et al

Poster (2011, December 08)

Mussel caging with Mytilus galloprovincialis has been successively used to monitor classic trace metal (Cd, Cu, Zn, Cr, Fe, Ni, Pb) pollution of Mediterranean coastal waters at spatial scales ranging from ... [more ▼]

Mussel caging with Mytilus galloprovincialis has been successively used to monitor classic trace metal (Cd, Cu, Zn, Cr, Fe, Ni, Pb) pollution of Mediterranean coastal waters at spatial scales ranging from 10 to 100km. However, its relevance as bioindicator at smaller scales (100 m - 1 km) is poorly known. Moreover, the levels of some little studied trace elements (Al, V, Mn, Sb, Sn, Ag, Mo, Se, As, Co, Be, Bi), recently identified as potential pollutants of coastal environments, have not yet been assessed in that species. In this work, M. galloprovincialis was used to monitor the 19 listed trace elements at the scale of the Calvi Bay (NW Corsica, France). Additionally, we investigated decontamination kinetics and trace element tissue speciation before and after spawning. Mytilus galloprovincialis trace element levels reflect the good water quality of the Calvi Bay, showing little spatial variations either at 100m or 1km scales. Filter feeders are only influenced by their relatively homogeneous pelagic environment (dissolved trace elements and suspended particulate matters), in contrast to organisms which inhabit typically heterogeneous benthic habitats. This bioindicator, a convincing candidate for the monitoring of the 12 little studied trace elements, effectively accumulates the 19 studied elements to 105 seawater concentrations. Mytilus galloprovincialis rapidly equilibrates (within days) with its environment, and is therefore a good indicator of chronic and stable chemical pollutions. Tissue speciation shows that the most relevant organ to monitor trace elements is the hepatopancreas. However, the important variability induced by the reproductive cycle of mussels requires using this bioindicator during its sexual dormancy. [less ▲]

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See detailProlongation de la durée de conservation de l'huile de cactus par ajout des huiles essentielles de quelques plantes aromatiques de la région du Sud marocain et d'huile d'Argan
Zine, S.; El Hadek, M.; Sindic, Marianne ULg

in 5èmes Journées Internationales d'Etude sur les Lipides - JIEL 2011 : Graisses alimentaires, Lipides marins, Nutrition et Santé (2011, December 08)

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