References of "2010"
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See detailPolymer maximum drag reduction: A unique transitional state
Dubief, Yves; White, Christopher M.; Shaqfeh, Eric S. G. et al

in Annual Research Briefs (2010)

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See detailCamelid single-domain antibody fragments as structural probes to study the mechanism of human lysozyme fibrils formation
Dumont, Janice ULg; Pardon, Els; Menzer, Linda ULg et al

Poster (2010)

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidosis. These ... [more ▼]

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidosis. These proteins form extracellular amyloid fibrils that deposit in a wide range of tissues and organs such as liver, spleen and kidneys where they cause damages [1]. It was shown that the D67H and I56T mutations cause a loss in stability and more particularly a loss of global cooperativity of protein [1]. Consequently, under physiologically relevant conditions, these variants can transiently populate a partially unfolded state in which the beta-domain and the C-helix are cooperatively unfolded while the rest of the protein remains native like [1]. The formation of intermolecular interactions between the regions that are unfolded in this intermediate state is likely to be a fundamental trigger of the aggregation process that ultimately leads to the formation and deposition of fibrils in tissues. The binding of three variable domain of camelid antibodies – also named nanobodies - (cAb-HuL 6 [2], cAb-HuL 5 and cAb-HuL 22 [3]) raised against the wild type human lysozyme inhibit in vitro the formation of amyloid fibrils by the lysozyme variants. These three nanobodies bind on different regions of lysozyme and act as amyloid fibrils inhibitor through different mechanisms. On one hand, cAb-HuL 6 and cAb-HuL 22 stabilize the native state of the lysozyme variants thus restoring the global cooperativity characteristic of the wild-type protein. On the other, cAb-HuL 5 probably acts by binding soluble prefibrillar aggregates. In the present work, sixteen other nanobodies specific of human lysozyme have been generated. Competition experiments have shown that they bind to five non overlapping epitopes. The effects of the binding of these nanobodies on the stability of the D67H variant of human lysozyme and on its aggregation into amyloid fibrils will be discussed. References [1] Dumoulin et al, (2006) Acc. Chem. Res, 39, 603-610. [2] Dumoulin et al, (2003) Nature, 424, 783-788. [3] Chan et al. (2008) Biochemistry, 47,11041-11054. [less ▲]

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See detailA subcritical damage model for clay as a two-scale material
François, Bertrand ULg; Dascalu, Cristian

in CLAYS IN NATURAL & ENGINEERED BARRIERS FOR RADIOACTIVE WASTE CONFINEMENT (2010)

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See detailLEGAL CERTAINTY v LEGAL PRECISION. Some thoughts on comparative law
Gerkens, Jean-François ULg

in Fundamina : A Journal of Legal History (2010), 16-1(2010), 121-129

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See detailConférences: Les rencontres du CEA en 2009
Van Ruymbeke, Muriel ULg

Report (2010)

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See detailSimulation of electronic sensing of biomolecules in translocation through a nanopore in a semiconductor membrane
Gracheva, Maria; Leroux, Amandine ULg; Destiné, Jacques ULg et al

in Iqbal, S. M.; Bashir, R. (Eds.) Nanopores: sensing and fundamental biological interactions (2010)

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See detailFull-system RANS of the HyShot II scramjet Part 1: Numerics and non-reactive simulations
Pecnik, Rene; Terrapon, Vincent ULg; Ham, Frank et al

in Annual Research Briefs (2010)

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See detailA time-dependent damage law in solids: a homogenization approach
Dascalu, Cristian; François, Bertrand ULg

in 9th HSTAM International Congress on Mechanics (2010)

The theoretical developments and the numerical applications of a time-dependent damage law is presented. This law is deduced from considerations at the micro-scale where non-planar growth of micro-cracks ... [more ▼]

The theoretical developments and the numerical applications of a time-dependent damage law is presented. This law is deduced from considerations at the micro-scale where non-planar growth of micro-cracks, following a subcritical propagation criterion, is assumed. The passage from micro-scale to macro-scale is done through an asymptotic homogenization approach. The model is built in two steps. First, the effective coefficients are calculated at the micro-scale in finite periodical cells, with respect to the micro-cracks length and their orientation. Then, a subcritical damage law is developed in order to establish the evolution of damage. As shown by numerical simulations, the developed model enables to reproduce the long-term behavior encountering relaxation and creep effects. [less ▲]

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See detailReport of the ECCO pathogenesis workshop on anti-TNF therapy failures in inflammatory bowel diseases: definitions, frequency and pharmacological aspects.
Allez, Matthieu; Karmiris, Konstantinos; Louis, Edouard ULg et al

in Journal of Crohn’s and Colitis [=JCC] (2010), 4(4), 355-66

The first ECCO pathogenesis workshop focused on anti-TNF therapy failures in inflammatory bowel diseases (IBDs). The overall objective was to better understand and explore primary non response and loss of ... [more ▼]

The first ECCO pathogenesis workshop focused on anti-TNF therapy failures in inflammatory bowel diseases (IBDs). The overall objective was to better understand and explore primary non response and loss of response to anti-TNF agents in IBD. The outcome of this workshop is presented into two parts. This first section addresses definitions, frequency and pharmacological aspects of anti-TNF therapy failure, including pharmacokinetics of anti-TNF monoclonal antibodies and immune and non-immune mediated clearance of anti-TNF mAbs. The second section concerns the biological roles of TNF and TNF antagonists, including mechanisms of action of anti-TNF agents, and discuss hypothesis regarding their failures and phenomenon of paradoxical inflammation, including the potential role of TNF independent inflammatory pathways. [less ▲]

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See detailΤο μετεωρο βημα του (νεου) αναλυτη
Naziri, Despina ULg

in Ἐκ τῶν ὑστέρων : ψυχαναλυτικὸ περιοδικό = Ek ton ysteron : Psychoanalytic Review = Ek ton ysteron : Revue Psychanalytique (2010), 21(2), 47-74

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See detailRic-8: un nuevo GEF con estructura tipo "armadillo"
Figueroa, Maximiliano ULg

Doctoral thesis (2010)

RIC-8 is a highly conserved cytosolic protein (63 KDa) initially identified in C. elegans as an essential factor in neurotransmitter release and asymmetric cell division. Two different isoforms have been ... [more ▼]

RIC-8 is a highly conserved cytosolic protein (63 KDa) initially identified in C. elegans as an essential factor in neurotransmitter release and asymmetric cell division. Two different isoforms have been described in mammals, RIC-8A and RIC-8B; each possess guanine nucleotide exchange activity (GEF) on heterotrimeric G-proteins, but with different Gα subunits specificities. To gain insight on the mechanisms involved in RIC-8 cellular functions it is essential to obtain some information about its structure. Therefore, the aim of this thesis was to study the relationship between structure and function on RIC-8, using as model RIC-8 from X. laevis. Analysis in its primary structure did not give us information about the function of xRIC-8 and RIC-8 proteins were showed as a single family without similarity with others. For this reason, to obtain a 3D model of xRIC-8, different bioinformatics approaches that include protein folding and structure prediction were used. The RIC-8 structural model is composed of 10 armadillo folding motifs, organized in a right-twisted alpha-alpha super helix. In order to validate the structural model, a His-tag fusion construct of RIC-8 was expressed in E. coli, purified by affinity and anion exchange chromatography and subjected to circular dichroism analysis (CD) and thermostability studies. This model together with the comparison among RIC-8 proteins that shows a high conservation in the carboxy region, deletion mutants that remove the last three armadillo domain were created in order to search a loss of the GEF function. The mutants could not be expressed in bacteria for in vitro assays, but their expression in HEK293T culture showed that all of them preserved the GEF activity. Furthermore, confocal microscopy showed that all the mutants could translocate to plasmatic membrane under stimulation with isoproterenol, and have the capacity to interact with Gs. The results of this thesis has allowed present the first 3D model for a RIC-8 protein, as well as classify RIC-8 as a new member of the protein family with armadillo repeats. Functionally, the carboxi terminal region, the most conserved among RIC-8, did not show has the GEF activity, and did not alter its behavior in HEK293T cell cultures while was stimulated with isoproterenol. [less ▲]

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See detailCerebral folate deficiency and CNS inflammatory markers in Alpers disease.
Hasselmann, Oswald; Blau, Nenad; RAMAEKERS, Vincent ULg et al

in Molecular Genetics & Metabolism (2010), 99(1), 58-61

We describe a 3.5-year-old female with Alpers disease with a POLG genotype of p.A467T/p.G848S and with a lethal outcome. Laboratory investigation revealed elevated CSF neopterin, IL-6, IL-8, IFN-gamma ... [more ▼]

We describe a 3.5-year-old female with Alpers disease with a POLG genotype of p.A467T/p.G848S and with a lethal outcome. Laboratory investigation revealed elevated CSF neopterin, IL-6, IL-8, IFN-gamma, reduced CSF 5-methyltetrahydrofolate (5MTHF), and increased serum as well as CSF folate receptor blocking autoantibodies. Treatment with oral Leucovorine (5-formyl-tetrahydrofolate) was initiated at 0.25mg/kg bid, and later increased to 4mg/kg bid. Under treatment CSF levels of 5MTHF, seizure frequency and communicative abilities improved. Over a time span of 17months, CSF levels of IL-6 and IFN-gamma decreased, levels of folate receptor blocking autoantibodies continued to raise, whereas CSF IL-8 remained elevated 1500-fold above normal. The child died without apparent stress at the age of 5.5years. Alpers disease, a neurodegenerative disease usually presents in the first years of life as a progressive encephalopathy with multifocal myoclonic seizures, developmental regression, cortical blindness and early death. The underlying genetic defect has been attributed to mutations of the catalytic subunit of the mitochondrial DNA polymerase-gamma leading to an organ-specific mitochondrial DNA depletion syndrome with reduced activity of respiratory chain enzyme complexes in the brain and the liver. A curative therapy is not available. This case report of Alpers disease provides new insights into the pathophysiology of Alpers disease, where mitochondrial dysfunction in conjunction with inflammatory cytokines and blocking folate receptor autoantibodies may lead to a secondary cerebral folate deficiency syndrome. The treatment of the latter provides relief to the patient without stopping the underlying disease. [less ▲]

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See detailHans von Reutlingen. Lambertus-Büstenreliquiar 1508-1512
George, Philippe ULg

in Renaissance am Rhein (2010)

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See detailMechanics of unsaturated soils
Laloui, Lyesse; Nuth, Mathieu; François, Bertrand ULg

in Laloui, Lyesse (Ed.) Mechanics of unsaturated materials (2010)

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See detailInfluence de la densité de la population sur la structure spatiale d’un paysage forestier dans le Bassin du Congo en R. D. Congo.
Bamba, I; Barima, Y S S; Bogaert, Jan ULg

in Tropical Conservation Science (2010), 3(1), 43-56

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See detailLe cas clinique du mois. Le lymphangiome kystique du mesentere
Verdin, Vanessa ULg; Seydel, Benoît ULg; Detry, Olivier ULg et al

in Revue Médicale de Liège (2010), 65(11), 615-8

Cystic lymphangioma of the mesentery is a benign condition, probably of malformative origin, and frequently appearing in infancy. Its symptomatology can be very polymorphic. Its diagnosis is suspected by ... [more ▼]

Cystic lymphangioma of the mesentery is a benign condition, probably of malformative origin, and frequently appearing in infancy. Its symptomatology can be very polymorphic. Its diagnosis is suspected by ultrasonography and computed tomography, and definitely confirmed by pathology. About a recent case of cystic lymphangioma of the mesentery diagnosed and operated on at the university hospital of Liege in an adult patient, the authors review its classification and its therapeutic strategy. Surgical resection is indicated in symptomatic cystic lymphangioma. [less ▲]

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See detailDéveloppement et utilisation d'un système 4D-SIG au support de la conservation du site de Calakmul (Mexique)
Van Ruymbeke, Muriel ULg; Cervera - Xicotencatl, Ariadna; Garcia - Moreno, Renata et al

Report (2010)

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