Isotope coded protein label quantification of serum proteins--comparison with the label-free LC-MS and validation using the MRM approach.

in Talanta (2010), 80(4), 1487-95

Protein quantification based upon mass spectrometry is gaining ground in diverse applications of biological and clinical relevance. The present article focuses on one of the most complex biological fluids ... [more ▼]

Protein quantification based upon mass spectrometry is gaining ground in diverse applications of biological and clinical relevance. The present article focuses on one of the most complex biological fluids - serum - and provides a novel ICPL based quantification protocol. The results are compared to a label-free (data independent alternate scanning) absolute quantification method. The validation is performed using MRM based protein quantification technique. Regarding the ICPL approach, serum samples used in this study were depleted of high abundant proteins, labeled with ICPL and fractionated according to their respective pI (3-5, 5-7 and 7-12). The samples were further subjected to tryptic digestion followed by treatment with the Glu-C enzyme. The peptides were analyzed on a 2D-nano-LC system using four different concentrations of salt injections (45, 75, 150 and 500 mM ammonium acetate). The LC system was connected on-line with the electrospray ion-trap mass spectrometer. For the label-free quantification the serum samples were depleted and digested with trypsin. A proteome-wide comparison was performed using highly reproducible LC and data independent alternate scanning in conjunction with a high mass accuracy orthogonal time-of-flight mass spectrometer. Selected proteins, found by both methods, were validated using the MRM approach. For this purpose non-depleted tryptically digested serum samples were analyzed by LC coupled with a triple-quadrupole MS. The relative protein quantification using ICPL and mass spectrometry allowed for the detection of approximately 200 proteins, whereas about 2/3 of those contained the ICPL label and could therefore be quantified. Label-free approach used no fractionation, less sample and was able to identify and quantify over 110 proteins. The identified proteins covered generally 3-4 orders of magnitude of protein concentration in human serum. Changes in relative abundance of eight proteins were validated using MRM. This study, for the first time, shows the ability of the relative protein quantification based upon ICPL and 2D-LC-MS/MS to quantify serum biomarkers. It provides two additional label-free approaches that could validate and bring additional value to the label-based results, offering a starting point for comprehensive proteomics studies aiming at revealing biomarkers of clinical relevance. [less ▲]

Gabriel Naudé entre bibliothèque docte et cabinet de curiosités

in Histoire et Civilisation du Livre : Revue Internationale (2010), VI

Nonlinear Normal Modes: Theoretical Curiosity or Practical Concept ?, KEYNOTE LECTURE,

in IMPACT 2010, Djerba, 2010 (2010)

Recollection and familiarity memory processes in probable Alzheimer's disease: an fMRI study

Scientific conference (2010)

Cerebral activity associated with recollection and familiarity in 28 patients with Alzheimer’s disease (AD) and 17 healthy controls was directly measured in an event-related fMRI experiment during ... [more ▼]

Cerebral activity associated with recollection and familiarity in 28 patients with Alzheimer’s disease (AD) and 17 healthy controls was directly measured in an event-related fMRI experiment during performance of a recognition memory task with the process dissociation procedure. Brain regions associated to recollection were evidenced by contrasting activations for inclusion and exclusion conditions whereas brain regions related to familiarity were explored with the mean effect of the two conditions (at P < .05 corrected). Twelve patients had null recollection estimates (AD-), whereas 16 patients did experience some recollection although significantly less than controls (AD+). In AD+ and controls, recollection activated the posterior cingulate cortex (PCC). In contrast, familiarity estimates were equivalent in the 3 groups and were associated with brain activations around the intraparietal sulcus (IPS). Thus, in AD, impaired recollection is related to damage of the PCC whereas preserved familiarity is supported by the IPS. [less ▲]

Réception d’un manuel scolaire d’histoire innovant : le cas de « Construire l’Histoire ». Étude de la motivation des enseignants d’histoire en Belgique francophone et au Grand-Duché de Luxembourg

in Education et Formation (2010), e-292

Néerlandais Presse Radio

Book published by De Boeck Université (2010)

method to improve the listening comprehension for the Dutch language (advanced level) - methode om de luistervaardigheid te verbeteren (de methode is gebaseerd op uitzendingen van de Vlaamse en ... [more ▼]

method to improve the listening comprehension for the Dutch language (advanced level) - methode om de luistervaardigheid te verbeteren (de methode is gebaseerd op uitzendingen van de Vlaamse en Nederlandse radio) [less ▲]

Rolnicy Warmii i Mazur w badaniach ankietowych belgijskiej studentki

in Bieżące Informacje (2010), 6(259), 38-39

Technisch rapport TR3 MP0.4 - Case study industriegebouw

Report (2010)

Ce rapport décrit le calcul de la résistance au feu d'un hall industriel sous feu naturel et contient trois parties : l'évaluation de la distribution de température dans le compartiment, l'échauffement de ... [more ▼]

Ce rapport décrit le calcul de la résistance au feu d'un hall industriel sous feu naturel et contient trois parties : l'évaluation de la distribution de température dans le compartiment, l'échauffement de la structure et sa stabilité. [less ▲]

Regards sur les industries laminaires du Paléolithique moyen en Asie centrale

Conference (2010)

Endogenous Growth and Parental Funding of Education in an OLG Model with a Fixed Factor

E-print/Working paper (2010)