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See detailAssociation between asthma control and bronchial hyperresponsiveness and airways inflammation: a cross-sectional study in daily practice.
Quaedvlieg, Valérie ULg; Sele, Jocelyne ULg; Henket, Monique ULg et al

in Clinical & Experimental Allergy : Journal of the British Society for Allergy & Clinical Immunology (2009), 39

Summary Background The primary end-point in the management of asthma is to obtain optimal control. The aim of this study was to assess the relationships between the markers of airway inflammation (sputum ... [more ▼]

Summary Background The primary end-point in the management of asthma is to obtain optimal control. The aim of this study was to assess the relationships between the markers of airway inflammation (sputum eosinophilia and exhaled nitric oxide), bronchial hyperresponsiveness (BHR) and asthma control. Methods One hundred and thirty-four patients were recruited from our asthma clinic between January 2004 and September 2005 [mean age: 42 years, mean forced expiratory volume in 1 s (FEV(1)): 86% predicted]. Eighty-six of them were treated by inhaled corticosteroids, 99 were atopic and 23 were current smokers. They all underwent detailed investigations including fractional-exhaled nitric oxide (FE(NO)) measurement, sputum induction and methacholine challenge when FEV(1) was >70% predicted, and filled in a validated asthma control questionnaire (ACQ6 Juniper). Results When dividing patients into the three groups according to their level of asthma control determined by ACQ [well-controlled asthma (ACQ score </=0.75), borderline (0.75<ACQ score <1.5) and uncontrolled asthma (ACQ score >/=1.5)], it appeared that uncontrolled asthmatics had a greater BHR to methacholine and sputum eosinophilia than controlled asthma (P<0.05, P<0.001, respectively). By contrast, we failed to show significant differences in the FE(NO) levels between the groups. With receiver-operating characteristic curves for differentiating uncontrolled (ACQ>/=1.5) from controlled and borderline (ACQ<1.5) asthma, sputum eosinophilia and methacholine responsiveness were found to be more accurate than FE(NO) (area under the curve: 0.72, 0.72 and 0.59, respectively). Conclusion In a broad spectrum of asthmatics encountered in clinical practice, sputum eosinophilia and methacholine bronchial hyperresponsiveness, but not FE(NO), are associated with uncontrolled asthma. [less ▲]

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See detailDam-break flow numerical modeling considering structural impacts on buildings
Erpicum, Sébastien ULg; Archambeau, Pierre ULg; Dewals, Benjamin ULg et al

in Proc. 33rd IAHR Congress: Water Engineering for a Sustainable Environment (2009)

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See detailBranching fractions and A values in singly ionized tantalum (Ta II)
Quinet, Pascal ULg; Fivet, V.; Palmeri, P. et al

in Astronomy and Astrophysics (2009), 493

Aims. We report on the theoretical and experimental lifetimes of Ta II, and calculated branching fractions for selected transitions. Methods. The theoretical data are obtained by means of a relativistic ... [more ▼]

Aims. We report on the theoretical and experimental lifetimes of Ta II, and calculated branching fractions for selected transitions. Methods. The theoretical data are obtained by means of a relativistic Hartree-Fock method with detailed attention to correlation effects. The experimental lifetimes are measured with the time-resolved, laser-induced, fluorescence technique. Results. The calculated results are in good agreement with both previously known and new experimental lifetimes. New transition probabilities have been deduced for a set of Ta II transitions of astrophysical interest. [less ▲]

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See detailUse of the “Radiosity Importance Concept” in the Error Estimation in Radiative Heat Transfer Design of Spacecraft
Vueghs, Pierre ULg; Rochus, Pierre ULg; de Koning, Hans-Peter et al

in 1st INTERNATIONAL CONFERENCE OF SPACE TECHNOLOGY - ICST 2009 (2009)

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See detailThe secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?
Mugnier, S.; Kervella, M.; Douet, C. et al

in Reproductive biology and endocrinology (2009), 7(1), 129

ABSTRACT: BACKGROUND: Oviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite ... [more ▼]

ABSTRACT: BACKGROUND: Oviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a role in fertilization. Thus, our second aim was to identify the proteins involved in fertilization in the horse. Methods & results In the first experiment, we co-incubated fresh equine spermatozoa treated with calcium ionophore and in vitro matured equine oocytes with or without porcine OEC. We showed that the presence of OEC increases the IVF rates. In the subsequent experiments, we co-incubated equine gametes with OEC and we showed that the IVF rates were not significantly different between 1) gametes co-incubated with equine vs porcine OEC, 2) intact cumulus-oocyte complexes vs denuded oocytes, 3) OEC previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone and/or oestradiol vs non stimulated OEC, 4) in vivo vs in vitro matured oocytes. In order to identify the proteins responsible for the positive effect of OEC, we first searched for the presence of the genes encoding oviductin, osteopontin and atrial natriuretic peptide A (ANP A) in the equine genome. We showed that the genes coding for osteopontin and ANP A are present. But the one for oviductin either has become a pseudogene during evolution of horse genome or has been not well annotated in horse genome sequence. We then showed that osteopontin and ANP A proteins are present in the equine oviduct using a surface plasmon resonance biosensor, and we analyzed their expression during oestrus cycle by Western blot. Finally, we co-incubated equine gametes with or without purified osteopontin or synthesized ANP A. No significant effect of osteopontin or ANP A was observed, though osteopontin slightly increased the IVF rates. CONCLUSION: Our study shows a beneficial effect of homologous and heterologous oviduct cells on equine IVF rates, though the rates remain low. Furthers studies are necessary to identify the proteins involved. We showed that the surface plasmon resonance technique is efficient and powerful to analyze molecular interactions during fertilization. [less ▲]

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See detailLa maîtrise de son corps par la personne. Concept et applications
Leleu, Yves-Henri ULg; Genicot, Gilles ULg; Langenaken, Evelyne ULg

in Les droits de la personnalité. Actes du Xe colloque de l'Association Famille et Droit organisé à Louvain-la-Neuve le 30 novembre 2007 (2009)

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See detailGuidelines for an integrated diagnostic approach of chronic lymphoproliferative disorders in the routine laboratory of haematology in Belgium.
Nollet, F.; Bakkus, M.; Meeus, P. et al

in Acta Clinica Belgica (2009), 64(6), 494-504

This paper summarizes the minimal workout of chronic lymphoproliferative disorders in a routine laboratory of haematology as recommended by a team of experienced laboratory supervisors in Belgium, taking ... [more ▼]

This paper summarizes the minimal workout of chronic lymphoproliferative disorders in a routine laboratory of haematology as recommended by a team of experienced laboratory supervisors in Belgium, taking into account the specific organisation of healthcare in Belgium, the innovations in the field of molecular analyses and related reimbursement. The starting point was essentially based upon clinical and/or haematological indications and it is emphasized that conclusions should be drawn in close dialogue with the clinician and experts in cytogenetics and histopathology. Reports made in the laboratory should be based upon an integration of cytomorphological, immunophenotypical and molecular data. These guidelines are not intended to be used as universal 'diagnostic pathways', but should be useful in developing local diagnostic pathways. It is well understood that this consensus, being valid anno 2009, may rapidly change with new technologies being introduced and new targets discovered. [less ▲]

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See detailL’aménagement du territoire et Environnement – Problématiques et impacts
Michel, Quentin ULg

Learning material (2009)

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See detailIdentification, cartographie et gestion des Forêts à Haute Valeur de Conservation : cas de la concession Pallisco, Cameroun
Julve Larrubia, C.; Sépulchre, F.; Philippart, Julien ULg et al

Conference (2009)

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See detailReseña de América Latina en la “literatura mundial”, de Ignacio Sánchez-Prado (ed.)
Willson, Patricia ULg

in Iberoamericana (2009), 9(34), 223-226

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See detailDe notre détermination à nous aveugler... (Introduction)
Strivay, Lucienne ULg

in Echanges, Le vivant en ville, Symposium International 2 (2009)

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See detailCollaboration industrielle et enseignement par projet en électronique de puissance
Plumier, Frédéric ULg; Bleus, Paul; Geuzaine, Christophe ULg

in J3eA : Journal sur l'Enseignement des Sciences et Technologies de l'Information et des Systèmes (2009), 8(Hors série 1), 1015

For the first time during year 2007-2008, a complete design of a DC/DC converter, based on specifications only, was asked to the students following the basic Power Electronics course at University of ... [more ▼]

For the first time during year 2007-2008, a complete design of a DC/DC converter, based on specifications only, was asked to the students following the basic Power Electronics course at University of Liège. This very realistic pedagogical way of teaching helped the students to be autonomous, to learn project and team work. [less ▲]

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See detailImpaired respiration discloses the physiological significance of state transitions in Chlamydomonas.
Cardol, Pierre ULg; Alric, Jean; Girard-Bascou, Jacqueline et al

in Proceedings of the National Academy of Sciences of the United States of America (2009), 106(37), 15979-84

State transitions correspond to a major regulation process for photosynthesis, whereby chlorophyll protein complexes responsible for light harvesting migrate between photosystem II and photosystem I in ... [more ▼]

State transitions correspond to a major regulation process for photosynthesis, whereby chlorophyll protein complexes responsible for light harvesting migrate between photosystem II and photosystem I in response to changes in the redox poise of the intersystem electron carriers. Here we disclose their physiological significance in Chlamydomonas reinhardtii using a genetic approach. Using single and double mutants defective for state transitions and/or mitochondrial respiration, we show that photosynthetic growth, and therefore biomass production, critically depends on state transitions in respiratory-defective conditions. When extra ATP cannot be provided by respiration, enhanced photosystem I turnover elicited by transition to state 2 is required for photosynthetic activity. Concomitant impairment of state transitions and respiration decreases the overall yield of photosynthesis, ultimately leading to reduced fitness. We thus provide experimental evidence that the combined energetic contributions of state transitions and respiration are required for efficient carbon assimilation in this alga. [less ▲]

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See detailElevated GH and normal IGF-1 : Discrepant biochemical results
Beckers, Albert ULg

Scientific conference (2009)

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See detailIdentification of anionic selenium species in Se-rich yeast by electrospray QTOF MS/MS and hybrid linear ion trap/orbitrap MSn
Dernovics, M.; Far, Johann ULg; Lobinski, R.

in Metallomics (2009), 1(4), 317-329

An analytical approach allowing the identification Of unknown selenium metabolites ill selenium-rich yeast was described. Anion-exchange HPLC of the Se-metabolome fraction co-eluting with salts ill size ... [more ▼]

An analytical approach allowing the identification Of unknown selenium metabolites ill selenium-rich yeast was described. Anion-exchange HPLC of the Se-metabolome fraction co-eluting with salts ill size-exclusion chromatography allowed the separation of nine selenium species (excluding isomers and selenate) as monitored by inductively Coupled plasma mass spectrometry (ICP MS). The individual fractions were analyzed by electrospray QTOF MS/MS and hybrid linear ion trap/Orbitrap MSn after sample introduction by reversed-phase nanoHPLC and by hydrophilic interaction LC (HILIC), respectively. Out of the nine detected species. eight were identified oil the basis of accurate mass measurements and collision induced dissociation/fragmentation information. Seven Se-species (selenohomolanthionine, gamma-Glu-selenocystathionine, 2,3-DHP-selenocystathionine, N-acetyl-selenocystathionine, 2,3-DHP-selenohomolanthionine Se-methyl-selenoglutathione, and 2,3-DHP-Se-methylselenocysteine) were reported for the first time in Se-rich yeast. five of them have never been reported in any biological sample before. [less ▲]

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See detailAnalyse des coûts/bénéfices dans le cadre de la lutte contre Diahotica virgifera Le Conte
Louppe, H.; Burny, Philippe ULg; De Proft, M. et al

Report (2009)

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See detailIntegrating Succession and Gender Issues from the Perspective of the Daughter of Family Enterprise across the U.S. and EU
Constantinidis, Christina ULg; Nelson, Teresa

in Management International = International Management = Gestión Internacional (2009), 14(1), 43-54

We engage a gender analysis, including consideration of the social phenomena of separation and hierarchy, to consider the role, condition and aspirations of daughters of family enterprise as they consider ... [more ▼]

We engage a gender analysis, including consideration of the social phenomena of separation and hierarchy, to consider the role, condition and aspirations of daughters of family enterprise as they consider the option of family entrepreneurship. From this perspective we examine the extant literature and present findings from two exploratory empirical research efforts, one in Belgium and one in the U.S., to better understand internationally how daughters perceive their leadership paths in the family business. We take into consideration growing social acceptance of women’s leadership, and women’s own career-mindedness, which influence traditional views of the opportunities for daughters inside and outside of the family business. Research avenues and practical recommendations to support successful succession of daughters are discussed. [less ▲]

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