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See detailEvaluation of the long-term barrier effect of commercial resorbable guided tissue regenerative membranes : an in vitro study using human gingival fibroblasts
Grenade, Charlotte ULg; Borget, Pascal; Moniotte, Nicolas et al

Poster (2009)

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The ... [more ▼]

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The second objective of our study was to develop an in vitro device able to measure the long-term barrier effect of resorbable membranes. After the development of this new device, experiments were realized to characterize the long-term behaviour of commercially membranes with human gingival fibroblasts (HGF). Materials and methods The use of human gingival fibroblastic cells was chosen to get closer to biological conditions. Some gingival explants were removed in young and non-smoking healthy patients. From these explants, fibroblastic cells were isolated and cultivated. These cells will be able to be used between the third and the sixth passage. Resorbable membranes were chosen because they don’t require a second surgical operation. There are made of polyesters or collagen. A system based on inserts was developed in order to follow the degradation of membranes and the migration of cells across the material. The membrane was cut into 8 mm diameter punches and set in the bottom of the system. Once the whole was put together, it is laced into a 12 wells plate culture. First, the plates were put in an incubator at 37°C, during times ranging from 24 hours to several months. The barrier effect was then measured to reflect the gradual increase of permeability of each membrane. For this purpose, HGF were seeded on the different samples. The top of the bottle and the bottom of the well were then filled with culture medium. Non degradable synthetic Bioflex membranes were chosen as control samples which don't let pass cells (porosity : 0,4 µm). After 48 hours of incubation in the presence of cells, pictures of cells on membranes and in the bottom of wells were taken with an optic microscope. Viability tests (MTS) were then realized on membranes to evaluate cells proliferation and in the bottom of wells to measure barrier effect. Finally, the morphology of cells on selected membranes was characterized by Scanning Electron Microscopy. Conclusion Proliferation results correspond to data published by several authors. Furthermore, the barrier effect times found in the present study are similar to barrier effect times demonstrated in in vivo studies and announced by manufacturers. In conclusion, the finalized system is adapted to the analysis of long-term barrier effect of commercial GTR membranes. This system will be tested with synthetic bioresorbable membranes made of copolymers. [less ▲]

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See detailAnnual report 2009 of the IGCP-580 Project.
Da Silva, Anne-Christine ULg; Whalen, Michael; Hladil, Jindrich et al

Report (2009)

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See detailEvaluating the impacts of a network on healthy eating promotion practices in schools
Vandoorne, Chantal ULg; Van Hoye, Aurélie ULg

in Archives of Public Health (2009), 67(Supplement 2), 72-73

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See detailSTFT-based spectral analysis of urban waterworks leakage detection
Lay-Ekuakille, Aimé; Vendramin, Giuseppe; Trotta, Amerigo et al

in XIX IMEKO World Congress Proceedings (2009)

Water is an essential good for human being necessity. It must be protected against pollution and useless leakage. Urban waterworks is a strategic infrastructure to be managed with particular attention and ... [more ▼]

Water is an essential good for human being necessity. It must be protected against pollution and useless leakage. Urban waterworks is a strategic infrastructure to be managed with particular attention and care so that users must be satisfied. Different techniques are used to detect leaks from waterworks, specifically from pipelines carrying water. Among them, spectral analysis is very interesting to apply amid problems related to the pipeline section. This paper presents an application of STFT (short-term Fourier transform) technique for identifying urban waterworks leaks; STFT technique is usually used in processing speech. For the purposes of this research, a specific pipeline plant has been build in other to feature a real case. The pipeline section is almost one inch; the adopted section makes the research very interesting since spectral analysis, based essentially on FFT, is generally used for section greater than 20 cm. [less ▲]

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See detailLa tutelle sur les marchés publics en Région wallonne
Durviaux, Ann-Lawrence ULg

Conference given outside the academic context (2009)

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See detailEffect of membrane permeability on survival of hemodialysis patients
Locatelli, Francesco; Martin-Malo, Alejandro; Hannedouche, Thierry et al

in Journal of the American Society of Nephrology [=JASN] (2009), 20

The effect of high-flux hemodialysis membranes on patient survival has not been unequivocally determined. In this prospective, randomized clinical trial, we enrolled 738 incident hemodialysis patients ... [more ▼]

The effect of high-flux hemodialysis membranes on patient survival has not been unequivocally determined. In this prospective, randomized clinical trial, we enrolled 738 incident hemodialysis patients, stratified them by serum albumin 4 and 4 g/dl, and assigned them to either low-flux or high-flux membranes. We followed patients for 3 to 7.5 yr. Kaplan-Meier survival analysis showed no significant difference between high-flux and low-flux membranes, and a Cox proportional hazards model concurred. Patients with serum albumin 4 g/dl had significantly higher survival rates in the high-flux group compared with the low-flux group (P 0.032). In addition, a secondary analysis revealed that high-flux membranes may significantly improve survival of patients with diabetes. Among those with serum albumin 4 g/dl, slightly different effects among patients with and without diabetes suggested a potential interaction between diabetes status and low serum albumin in the reduction of risk conferred by high-flux membranes. In summary, we did not detect a significant survival benefit with either high-flux or low-flux membranes in the population overall, but the use of high-flux membranes conferred a significant survival benefit among patients with serum albumin 4 g/dl. The apparent survival benefit among patients who have diabetes and are treated with high-flux membranes requires confirmation given the post hoc nature of our analysis. [less ▲]

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See detailLes linguistes et leurs graphiques. Introduction
Badir, Sémir ULg

in Cahiers Parisiens = Parisian Notebooks (2009), 5

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See detailMonitoring an Odour in the Environment with an Electronic Nose: Requirements for the Signal Processing
Romain, Anne-Claude ULg; Nicolas, Jacques ULg

in Gutierrez, Agustin; Marco, S. (Eds.) Biologically Inspired Signal Processing for Chemical Sensing (2009)

Artificial olfaction system (the so-called electronic nose) is a very promising tool to monitor the malodour in the field. Usual odour measurement techniques use human olfaction or analytical techniques ... [more ▼]

Artificial olfaction system (the so-called electronic nose) is a very promising tool to monitor the malodour in the field. Usual odour measurement techniques use human olfaction or analytical techniques. The first category represents the real odour perception but is not applicable to measure in continuous bad odours in the field. The second class of techniques gives the composition of the mixture but not the global information representative of the odour perception. The e-nose has the potentialities to combine “the odour perception” and the “monitoring in the field”. However to be able to reach this goal, the signal processing has to be adapted to work in complex environment. We have more than teen years experiments in the measure of environmental malodours in the field and the paper presents the minimal requirements that we consider essential for artificial olfaction system to become successful for this application. [less ▲]

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See detailEstimation of genetic parameters and genome scan for 15 semen characteristics traits of Holstein bulls.
Druet, Tom ULg; Fritz, S.; Sellem, E. et al

in Journal of Animal Breeding & Genetics (2009), 126(4), 269-77

A QTL detection experiment was performed in French dairy cattle to search for QTL related to male fertility. Ten families, involving a total of 515 bulls, were phenotyped for ejaculated volume and sperm ... [more ▼]

A QTL detection experiment was performed in French dairy cattle to search for QTL related to male fertility. Ten families, involving a total of 515 bulls, were phenotyped for ejaculated volume and sperm concentration, number of spermatozoa, motility, velocity, percentage of motile spermatozoa after thawing and abnormal spermatozoa. A set of 148 microsatellite markers were used to realize a genome scan. First, genetic parameters were estimated for all traits. Semen production traits were found to have moderate heritabilities (from 0.15 to 0.30) while some of the semen quality traits such as motility had high heritabilities (close to 0.60). Genetic correlations among traits showed negative relationships between volume and concentration and between volume and most quality traits such as motility or abnormal sperm while correlations between concentration and these traits were rather favourable. Percentages of abnormal sperm were negatively related to quality traits, especially with motility and velocity of spermatozoa. Three QTL related to abnormal sperm frequencies were significant at p < 0.01. In total, 11 QTL (p < 0.05) were detected. However, the number of QTL detected was within the range of expected false positives. Because of the lack of power to find QTL in this design further analyses are required to confirm these QTL. [less ▲]

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

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See detailBiopsies rectales chez le cheval : technique, innocuité, et valeur diagnostique de l'examen histopathologique.
Leroux, Aurélia ULg

Master of advanced studies dissertation (2009)

La biopsie rectale est couramment utilisée sur les chevaux présentant un amaigrissement, de la diarrhée chronique ou des coliques récidivantes. Le but de cette étude est la mise au point de cette ... [more ▼]

La biopsie rectale est couramment utilisée sur les chevaux présentant un amaigrissement, de la diarrhée chronique ou des coliques récidivantes. Le but de cette étude est la mise au point de cette technique, l’étude de son innocuité et de sa valeur diagnostique. Huit biopsies rectales sont prélevées en différents endroits, avec la pince à biopsie utérine de la clinique, sur chacun des 12 chevaux de l’étude, 7 malades et 5 témoins, sous anesthésie générale ou simple sédation. Des prélèvements de duodénum, iléon, caecum, colon et rectum sont effectués post mortem sur chaque cheval. Un examen histopathologique sur lames colorées à l’hématoxyline-éosine, est réalisé. Pour un droitier, il est plus facile de prélever dans le cadran supérieur droit. Les autopsies montrent que 91% des biopsies à 30 cm de l’anus et 48% de celles à 15 cm sont localisées en avant du cul-de-sac rétropéritonéal. Des hématomes sont fréquents (44%) surtout ventralement. Une seule perforation est observée. Au microscope, l’inflammation sur les biopsies rectales est plus marquée à 30 cm et chez les chevaux malades. Des études similaires avec la méthode proctoscopique et avec une pince à biopsie utérine de plus petit calibre, seraient intéressantes en vue de comparer les résultats. [less ▲]

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See detailImpacts of the carbonyl group location of ester bond on interfacial properties of sugar-based surfactants: experimental and computational evidences
Razafindralambo, Hary ULg; Blecker, Christophe ULg; Mezdour, Samir et al

in Journal of Physical Chemistry B (2009), 113

Interfacial properties of surfactants are dependent on the conformation adopted by the hydrophilic headgroup or/and the hydrophobic tail at the boundary limit of two immiscible phases. Here, we ... [more ▼]

Interfacial properties of surfactants are dependent on the conformation adopted by the hydrophilic headgroup or/and the hydrophobic tail at the boundary limit of two immiscible phases. Here, we demonstrate the impacts of the carbonyl group (-CO-) location of the ester bond of sugar-based surfactants by comparing some properties of two closely related esters, octyl glucuronate and glucose octanoate, at the air-water interface. The carbonyl group location influences the rate and extent of interfacial adsorption and the rheology properties of sugar esters at the air-water interface, which were evaluated by dynamic surface tension and complex surface viscoelastic measurements. Octyl glucuronate adsorbs the fastest at the air-water interface whereas glucose octanoate reduces the dynamic surface tension at the lowest value and exhibits the highest film viscoelasticity. Differences are attributed to molecular conformation constraints inducing relevant changes to the surface coverage kinetic capacity and the interaction strengths of the octyl sugar ester adsorbed films at the air-water interface. All of the results are supported by the minimum cross-sectional area values per molecule determined by both experimental and computational approaches. [less ▲]

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See detailTraçabilité en agroalimentaire : pourquoi ? Comment ?
Duquesne, Brigitte ULg

in Origine et Traçabilité : actes du colloque (2009)

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See detailConcentrations of pregnancy-associated glycoproteins in Water buffaloes females (Bubalus bubalis) during pregnancy and postpartum periods
Barbato, O.; Melo de Sousa, Noelita ULg; Malfatti, A. et al

in O’Leary, M.; Arnett, J. (Eds.) Pregnancy Protein Research (2009)

The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708 ... [more ▼]

The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708). Samples (10 mL) were collected by jugular venipuncture from Week 0 until Week 28 of pregnancy (9 females), and from parturition until Week 10 postpartum (13 females). During pregnancy, PAG molecules were detectable at Week 6 using the three aforementioned RIA systems (3.9 ± 1.3 ng/mL, 9.7 ± 1.3 ng/mL, and 9.9 ± 0.7 ng/mL for RIA-497, RIA-706, and RIA-708, respectively). These concentrations increased gradually until Week 28, reaching values of 39.6 ± 4.0 ng/mL (RIA-497), 50.5 ± 11.9 ng/mL (RIA-706), and 68.2 ± 20.8 ng/mL (RIA-708). PAG concentrations determined by RIA-497, RIA-706, and RIA-708 were strongly correlated throughout the entire gestation period, RIA-708 giving the higher concentrations. At parturition, the mean concentrations ranged from 34.9 ± 4.0 (RIA-497) to 84.7 ± 10.6 ng/mL (RIA-708). Thereafter, the concentrations decreased steadily, reaching very low levels (< 1.0 ng/mL) at Week 8 postpartum. In conclusion, PAG concentrations measured by the above three RIA systems showed a profile similar to those previously described in bovine species, with higher concentrations being detected by RIA-706 and RIA-708. However, the patterns of PAG concentrations, studied using the three aforementioned PAG-RIA systems, differed around parturition, with very low levels being observed in the female buffaloes [less ▲]

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See detailGrain boundary effects in bulk colossal magneto resistive (CMR) manganites and manganite/insulator composites: electrical and magnetic properties
Vanderbemden, Philippe ULg; Vertruyen, Bénédicte ULg; Ausloos, Marcel ULg et al

in Journal of Optoelectronics and Advanced Materials [= JOAM] (2009), 11(9), 1115-1121

In the first part of this paper, we discuss the effects of grain boundaries on the properties of bulk colossal magnetoresistive (CMR) manganites We compare the electrical resistivity and AC magnetic ... [more ▼]

In the first part of this paper, we discuss the effects of grain boundaries on the properties of bulk colossal magnetoresistive (CMR) manganites We compare the electrical resistivity and AC magnetic susceptibility of perovskite La-Ca-Mn-O samples with the same nominal stoichiometry but differing in their microstructure (i) a single grain sample, (ii) a sample containing two grains and (iii) a polycrystalline sample Emphasis is placed on information that can be deduced from the measurements in each case In the second part of the paper, we report the data measured on composite samples containing a CMR phase (La-Ca-Mn-O) and an insulating phase (Mn3O4) The results are discussed in the framework of percolation theory We show how the grain boundaries affect the electrical properties of these materials, and we highlight the crucial role of geometric (demagnetization) effects on the resistance vs magnetic field measurements [less ▲]

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See detailA flamelet-based model for supersonic combustion
Terrapon, Vincent ULg; Ham, Frank; Pecnik, Rene et al

in Annual Research Briefs (2009)

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See detailMineralogy and petrography of phosphate minerals from Sapucaia and Boca Rica pegmatites, Minas Gerais, Brazil
Baijot, Maxime ULg; Hatert, Frédéric ULg; Philippo, Simon et al

in Estudios Geologicos (2009), 19(2), 47-51

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