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See detailPutative DNA G-quadruplex formation within the promoters of Plasmodium falciparum var genes
Smargiasso, Nicolas ULg; Gabelica, Valérie ULg; Damblon, Christian ULg et al

in BMC Genomics (2009), 10

Background. Guanine-rich nucleic acid sequences are capable of folding into an intramolecular four-stranded structure called a G-quadruplex. When found in gene promoter regions, G-quadruplexes can ... [more ▼]

Background. Guanine-rich nucleic acid sequences are capable of folding into an intramolecular four-stranded structure called a G-quadruplex. When found in gene promoter regions, G-quadruplexes can downregulate gene expression, possibly by blocking the transcriptional machinery. Here we have used a genome-wide bioinformatic approach to identify Putative G-Quadruplex Sequences (PQS) in the Plasmodium falciparum genome, along with biophysical techniques to examine the physiological stability of P. falciparum PQS in vitro. Results. We identified 63 PQS in the non-telomeric regions of the P. falciparum clone 3D7. Interestingly, 16 of these PQS occurred in the upstream region of a subset of the P. falciparum var genes (group B var genes). The var gene family encodes PfEMP1, the parasite’s major variant antigen and adhesin expressed at the surface of infected erythrocytes, that plays a key role in malaria pathogenesis and immune evasion. The ability of the PQS found in the upstream regions of group B var genes (UpsB-Q) to form stable Gquadruplex structures in vitro was confirmed using 1H NMR, circular dichroism, UV spectroscopy, and thermal denaturation experiments. Moreover, the synthetic compound BOQ1 that shows a higher affinity for DNA forming quadruplex rather than duplex structures was found to bind with high affinity to the UpsB-Q. Conclusions. This is the first demonstration of non-telomeric PQS in the genome of P. falciparum that form stable G-quadruplexes under physiological conditions in vitro. These results allow the generation of a novel hypothesis that the G-quadruplex sequences in the upstream regions of var genes have the potential to play a role in the transcriptional control of this major virulence-associated multi-gene family. [less ▲]

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See detailImpaired respiration discloses the physiological significance of state transitions in Chlamydomonas.
Cardol, Pierre ULg; Alric, Jean; Girard-Bascou, Jacqueline et al

in Proceedings of the National Academy of Sciences of the United States of America (2009), 106(37), 15979-84

State transitions correspond to a major regulation process for photosynthesis, whereby chlorophyll protein complexes responsible for light harvesting migrate between photosystem II and photosystem I in ... [more ▼]

State transitions correspond to a major regulation process for photosynthesis, whereby chlorophyll protein complexes responsible for light harvesting migrate between photosystem II and photosystem I in response to changes in the redox poise of the intersystem electron carriers. Here we disclose their physiological significance in Chlamydomonas reinhardtii using a genetic approach. Using single and double mutants defective for state transitions and/or mitochondrial respiration, we show that photosynthetic growth, and therefore biomass production, critically depends on state transitions in respiratory-defective conditions. When extra ATP cannot be provided by respiration, enhanced photosystem I turnover elicited by transition to state 2 is required for photosynthetic activity. Concomitant impairment of state transitions and respiration decreases the overall yield of photosynthesis, ultimately leading to reduced fitness. We thus provide experimental evidence that the combined energetic contributions of state transitions and respiration are required for efficient carbon assimilation in this alga. [less ▲]

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See detailThe Quixote in the Stories of Subcomandante Marcos
Vanden Berghe, Kristine ULg

in D'haen, Theo; Dhondt, Reindert (Eds.) International Don Quixote (2009)

The principal spokesman of the Zapatista Army of National Liberation (Mexico, 1994), Subcomandante Marcos, devotes himself simultaneously to armed resistance and literature. Some of the stories he wrote ... [more ▼]

The principal spokesman of the Zapatista Army of National Liberation (Mexico, 1994), Subcomandante Marcos, devotes himself simultaneously to armed resistance and literature. Some of the stories he wrote are about a speaking, anti-neoliberal beetle, Don Durito de la Lacandona, who presents himself as an emulator of Don Quixote. The plays with Cervantes’ novel allow Marcos to spread his personal model about the hierarchy in the Zapatista guerrilla war, to dismiss the importance of authorship in the ranks of the revolutionary group and to enable every reader, according to his or her own personal horizon of reading, to actualize the figure of the beetle. It has been suggested that the tales about Durito are a pastiche of the novel of chivalry. We can add to this that, in the same way as Cervantes rewrote old genres by parodying them, the texts about Durito, as far as they are a sincere homage to the Quixote, move away from the traditional engaged literature in Latin America. [less ▲]

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See detailEarly identification of inner pillar cells during rat cochlear development.
Thelen, Nicolas ULg; Breuskin, Ingrid ULg; Malgrange, Brigitte ULg et al

in Cell & Tissue Research (2009), 337(1), 1-14

Although the structure of the auditory organ in mature mammals, the organ of Corti, is clearly established, its development is far from being elucidated. Here, we examine its spatio-temporal development ... [more ▼]

Although the structure of the auditory organ in mature mammals, the organ of Corti, is clearly established, its development is far from being elucidated. Here, we examine its spatio-temporal development in rats from embryonic day 16 (E16) to E19 by using cytochemical and immunocytochemical methods at the light- and electron-microscope levels. We demonstrate that the organ of Corti develops from a non-proliferating cell zone that is located in the junctional region between two edges of the dorsal epithelium of the cochlear duct. We also reveal that the first cells to develop in this zone are the inner pillar cells, a particular type of non-sensory supporting cell, which arise in the base of the cochlear duct at the boundary between the two ridges at E16. Cell differentiation in this prosensory region continues according to a base-to-apex gradient; the inner hair cells appear in the greater epithelial ridge at E17 and the outer hair cells in the lesser epithelial ridge at E18. At E19, the various cell types of the organ of Corti are in place. Finally, we show that unlike the development of all the supporting cell types of the organ of Corti, the development of inner pillar cells within the prosensory domain seems not to involve Notch1 activation. These results highlight the central role that the inner pillar cells probably play in the development of the organ of Corti. [less ▲]

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See detailAntigone et Manon s’invitent en droit social. Quelques propos sur la légalité de la preuve
Kefer, Fabienne ULg

in Revue Critique de Jurisprudence Belge (2009), 3

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See detailLe jeu de l'acteur: une nouvelle méthode pour aborder l'affirmation de soi. Résultats d'une première étude portant sur trois groupes et follow-up
Delvaux, Muriel ULg; Sarto, Danielle; Roodthooft, S. et al

in Revue Francophone de Clinique Comportementale et Cognitive (2009)

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See detailLa traduction dans l'antiquité
Rochette, Bruno ULg

Article for general public (2009)

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See detailQuestions sur le questionnement des pratiques
Van Beveren, Julien ULg

in La Lettre de l'AIRDF (2009), 44

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See detailPolynomial approximation of aerodynamic coefficients based on the statistical description of the wind incidence
Denoël, Vincent ULg

in Probabilistic Engineering Mechanics (2009), 24(2), 179-189

In civil engineering applications, the aerodynamic coefficients are usually measured in wind tunnels for several wind incidences. The measurement results need to be linearized in order to perform the ... [more ▼]

In civil engineering applications, the aerodynamic coefficients are usually measured in wind tunnels for several wind incidences. The measurement results need to be linearized in order to perform the design of the structure. This paper justifies the use of different linearization techniques for different assessments as divergence or buffeting analysis. In this latter context, it is proposed to linearize the aerodynamic coefficient by the least-square method, using the probability density function of the wind incidence as a weighting function. First this probability density function is computed for a 2-D wind flow, as a function of the wind intensities and their correlation. Then, the comparison of results from different linearization techniques provides surprising results indicating that what is usually performed should be considered with care. [less ▲]

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See detailHumanizing the Clinical Gaze: Movies and the Empathic Understanding of Psychosis
Raballo, A.; Laroi, Frank ULg; Bell, V.

in Family Medicine (2009), 41(6), 387-388

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See detailTesting and modeling a scroll expander integrated into an Organic Rankine Cycle
Lemort, Vincent ULg; Quoilin, Sylvain ULg; Cuevas, Cristian et al

in Applied Thermal Engineering (2009), 29

Organic Rankine Cycles (ORC’s) are particularly suitable for recovering energy from low-grade heat sources. This paper first presents the results of an experimental study carried out on a prototype of an ... [more ▼]

Organic Rankine Cycles (ORC’s) are particularly suitable for recovering energy from low-grade heat sources. This paper first presents the results of an experimental study carried out on a prototype of an open-drive oil-free scroll expander integrated into an ORC working with refrigerant HCFC-123. By exploiting the overall expander performance measurements, the eight parameters of a scroll expander semi-empirical model are then identified. The model is able to compute variables of first importance such as the mass flow rate, the delivered shaft power and the discharge temperature, and secondary variables such as the supply heating-up, the exhaust cooling-down, the ambient losses, the internal leakage and the mechanical losses. The maximum deviation between the predictions by the model and the measurements is 2% for the mass flow rate, 5% for the shaft power and 3 K for the discharge temperature. The validated model of the expander is finally used to quantify the different losses and to indicate how the design of the expander might be altered to achieve better performances. This analysis pointed out that the internal leakages and, to a lesser extent, the supply pressure drop and the mechanical losses are the main losses affecting the performance of the expander. [less ▲]

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See detailDevelopment of a strategy to study toxicodynamic of pollutants in spawning sea turtles
Dyc, Christelle ULg; Bouquegneau, Jean-Marie ULg; Gillet, Marie-Claire ULg et al

in Proceedings of the 29th Annual Symposium on Sea Turtle Biology and Conservation (2009)

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See detailL'efficacité de l'ibandronate oral mensuel est maintenue sur 5 ans : l'étude MOBILE extension long terme (ELT)
Chapurlat, R.; Felsenberg, F.; Reginster, Jean-Yves ULg

in Revue du Rhumatisme (2009)

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See detailSociologie post-critique et approche post-rawlsienne de la justice. Pour une théorie englobante du sens de la justice
Nachi, Mohamed ULg

in M. Breviglieri, Cl. Lafaye et D. Trom (Ed.) Compétences critiques et sens de la justice. Colloque de Cerisy (2009)

Il s'agit de comparer les théories de la justice à la sociologie pragmatique du juste pour montrer l'intérêt de cette dernière pour la compréhension du sens ordinaire de la justice

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See detailAlcohol consumption and the prevalence of metabolic syndrome : a meta-analysis of observational studies
Alkerwi, A; Boutsen, M; Vaillant, M et al

Poster (2009)

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See detailMap and determinants of woodlands visiting in Wallonia
Colson, Vincent ULg; Garcia, S.; Rondeux, Jacques ULg et al

in Urban Forestry and Urban Greening (2009)

The Walloon forest taken as a whole can be regarded as typical of a rural area, although its proximity to densely populated areas gives it a peri-urban character. It is visited by the local population as ... [more ▼]

The Walloon forest taken as a whole can be regarded as typical of a rural area, although its proximity to densely populated areas gives it a peri-urban character. It is visited by the local population as well as by tourists (including from neighboring countries). To provide spatial information on the level of Wallonia woodland visitation, a survey was conducted among managers of Forest Service districts (also called ‘‘cantonnements’’). The aim was to map the woodlands to show spatial patterns of visitation levels, and analyze them qualitatively to determine the influence factors. This map identifies regional hubs of recreation woodlands and, conversely, areas where the recreational function is much less important. The level of visitation was scaled in four levels ranging from low to very high. The mapping was supplemented with a statistical analysis of data collected from the forest managers and also from different GIS-layers (slope, hydrology, land use, etc.). An equation using the level of visitation as a dependent variable was fitted to a set of characteristics of the woodland with an ordered Logit model. The results show that type of ownership, type of forest, and recreational facilities significantly influence the level of woodland visitation. They also show that woodland visitors prefer any type of forest to mainly coniferous woodland. These results and the analysis of the map are particularly useful for developing forest policy and tourism as well as managing the forest. [less ▲]

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See detailInfluence of source and levels of dietary fiber on in vivo nitrogen excretion pathways in pigs and in vitro fermentation and protein synthesis by fecal bacteria
Bindelle, Jérôme ULg; Buldgen, André; Delacollette, Maud et al

in Journal of Animal Science (2009), 87

The inclusion of dietary fiber (DF) in diets has been suggested as a way to reduce ammonia emission in pig barns because it contributes to a shift in N excretion from urine to feces due to enhanced ... [more ▼]

The inclusion of dietary fiber (DF) in diets has been suggested as a way to reduce ammonia emission in pig barns because it contributes to a shift in N excretion from urine to feces due to enhanced bacterial growth in the intestines. This study compared an in vitro method to measure bacterial protein synthesis during fermentation to in vivo N excretion shift induced by diets differing in DF concentrations and solubility. The first experiment measured the effect of graded concentrations of sugar beet pulp (SBP; 0, 10, 20 and 30%) in corn-soybean meal-based diets on in vivo N excretion partitioning between urine and feces. A second experiment investigated the replacement of SBP, rich in soluble DF, by oat hulls (OH), rich in insoluble DF (20:0; 10.5:10.5; 0:22%, respectively). In parallel, the fermentation characteristics of the dietary carbohydrates not digested in the small intestine was evaluated in an in vitro gas test, based on their incubation with colonic microbiota, using a mineral buffer solution enriched with 15N. The N originating from the buffer solution incorporated into the bacterial proteins (BNI) was measured when half of the final gas volume was produced (T/2: 8.5 to 14.5 h of fermentation) and after 72 h of fermentation. Short-chain fatty acids (SCFA) were determined in the liquid phase. In the first experiment, the inclusion of SBP linearly decreased urinary N excretion from 0.285 to 0.215 g N excreted in urine per g N ingested and urinary-N:fecal–N excretion ratio from 2.171 to 1.177 (P < 0.01). In the second experiment, the substitution of SBP by OH linearly increased urinary-N:fecal–N excretion ratio (P = 0.009). Unlike SCFA production, BNI was greater at T/2 than at 72 h of fermentation. Sugar beet pulp enhanced BNI linearly (P < 0.001): 2.01, 2.06 and 2.35 mg g-1 diet with 10, 20 and 30% SBP, respectively, as compared to 1.51 mg for the control diet. The substitution of SBP by OH decreased BNI (P < 0.01). With the exception of final gas production, all in vitro kinetics characteristics and BNI were correlated to in vivo N excretion parameters and regression equations for the prediction of N excretion pathways from in vitro data were identified. Even if the presence of resistant starch in the diet might alter the composition of the fibrous residue that is fermented, it can be concluded that the in vitro method is a possible useful tool for the formulation of diets reducing the effects of pig production on the environment. [less ▲]

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See detailTraitement automatique des langues et langues anciennes
Denooz, Joseph ULg; Rosmorduc, Serge

in Traitement automatique des langues (2009), 50

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See detailImportance of steroid receptor coregulators for neuronal phenotype determination: Modulation of steroid action
Charlier, Thierry ULg

in Trabajos del Instituto Cajal (2009), LXXXII

Steroid receptors such as estrogen receptors alpha and beta and androgen receptors are transcription factors involved in the transcriptional regulation of a large number of target genes. Steroid-dependent ... [more ▼]

Steroid receptors such as estrogen receptors alpha and beta and androgen receptors are transcription factors involved in the transcriptional regulation of a large number of target genes. Steroid-dependent expression in the brain controls a large array of biological processes including spatial cognition, copulatory behavior and neuroprotection. The discovery of a competition, or squelching, between two different nuclear receptors introduced the notion that common cofactors might be involved in the modulation of transcriptional activity of nuclear receptors. These cofactors, which are now known as coactivators, are involved in chromatin remodeling and stabilization of the general transcription machinery. Since the characterization of the steroid receptor coactivator 1 or SRC-1, more than 100 different cofactors have been identified. Although an increasingly large amount of information has been collected about the in vitro function of these coregulatory proteins, relatively little is known regarding their physiological role in vivo, particularly in the brain. Our laboratory and others have demonstrated the importance of SRC-1 in the differentiation and activation of steroid-dependent sexual behaviors and the related neural genes. In Japanese quail, the inhibition of SRC-1 expression by intracerebroventricular antisense injections blocked the activating effects of exogenous testosterone on male sexual behaviors and the steroid-dependent vasotocine expression and increase of the median preoptic area volume defined by Nissl staining as well as by aromatase immunoreactivity. These data therefore strongly suggested that SRC-1 is required to modulate estrogen receptor dependent gene-expression. It is however interesting to note that steroid receptors and SRC-1 are not always colocalized. For example, both glial cells and neurons in the hippocampus express estrogen receptor alpha but SRC-1 is rarely observed in glia. It is therefore possible that estrogen receptor alpha in glial cell require another coactivator or set of coactivators to induce estrogen-dependent gene transcription. It has been suggested very recently that SRC-1 is associated with neuronal differentiation of neural stem cell derived from the ganglionic eminence of mouse embryos. These stem cells differentiating into glial cell (GFAP-positive) did not express SRC-1. The presence of a specific coactivator could therefore determine a specific cell phenotype (neuronal vs glial). Another coactivator, the coactivator-associated arginine methyl transferase 1 or CARM-1 seems to be important to keep progenitor cells in a dividing state. The inhibition of CARM-1 expression leads to neuronal differentiation. Neurogenesis can therefore offers an excellent model to define the spatio-temporal role of different coactivators. It is indeed possible to study a subset of coactivators associated to various stages phenotype determination (proliferation vs. differentiation). The study of neurogenesis in the dentate gyrus of the hippocampus in female adult rats shows that around 40 % of proliferative cells express SRC-1 or CARM-1. Interestingly, 70% of proliferative cells express SRC-1 but only a very few cells (<5%) express CARM-1. We are currently investigating the temporal pattern of expression of these two coactivators during the neurogenesis in the hilus and dentate gyrus. The expression of the coactivators CARM-1 and SRC-1 is analyzed in proliferating and differentiating cells. We expect that proliferating and differentiating cells will differentially express the two coactivators. It seems that the presence of a precise subset of coactivators could help defining the phenotype of the cell by modulating a specific downstream pathway after steroid receptor activation. The very large number of coactivators and their association into preformed complexes potentially allows the determination of hundreds of different phenotypes. The study of the expression of the coactivator and their function in vivo is required to fully understand steroid action and specificity in the brain. [less ▲]

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