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See detailFurther data on the lichen genus Bacidia s. l. in the Canary Islands and Western Europe, with descriptions of two new species
Brand, Maarten; Coppins, Brian J.; van den Boom, Pieter P. G. et al

in Bibliotheca Lichenologica (2009), 99

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See detailThe Worldwide Making of the Social Economy. Innovations and Changes
Defourny, Jacques ULg; Develtere, Patrick; Fonteneau, Bénédicte et al

Book published by Acco (2009)

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See detailSynthesis of titanium dioxide nanoparticles by reactive DC magnetron sputtering
Dreesen, Laurent ULg; Colomer, Jean-François; Limage, Hervé et al

in Thin Solid Films (2009), 518

Nanometer-sized titanium dioxide (TiO2) particles were prepared on carbon substrates by reactive direct-current magnetron sputtering. By performing measurements with high resolution electron microscopes ... [more ▼]

Nanometer-sized titanium dioxide (TiO2) particles were prepared on carbon substrates by reactive direct-current magnetron sputtering. By performing measurements with high resolution electron microscopes, the mean nanoparticle diameter and the coverage fraction of the substrate by the nanoparticles (NPs) were measured at 19 nm and 30%, respectively. Moreover, electron diffraction analysis showed that the TiO2 NPs' crystalline structure on the carbon substrate was a mixture of anatase and rutile. Finally,we provided information on the TiO2 initial growth stage: crystalline NPs were formed after deposition of amorphous nanoparticles on the substrate and heating. [less ▲]

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See detailHerpesviruses in respiratory liquids of horses : putative implication in airway inflammation and association with cytological features
Fortier, Guillaume ULg; Van Erck, Emmanuelle ULg; Fortier, Christine et al

in Veterinary Microbiology (2009), 139

The objectives of this study were to estimate the prevalence and the potential role of equine herpesviruses (EHVs) detection in both bronchoalveolar lavage (BAL) and tracheal wash (TW). The population ... [more ▼]

The objectives of this study were to estimate the prevalence and the potential role of equine herpesviruses (EHVs) detection in both bronchoalveolar lavage (BAL) and tracheal wash (TW). The population included a control group (CTL; 37 TW and 25 BAL) and a pathological group (PAT; 259 TW and 387 BAL), including horses either suffering from respiratory diseases including syndrome of tracheal inflammation, inflammatory airway disease, recurrent airway obstruction, or submitted to respiratory investigation because of exercise intolerance or poor performance. Each respiratory liquid was submitted to a standardised cytological analysis, mentioning the morphological abnormalities of exfoliated epithelial cells (ECAb) and ciliocytophthoria (CCPh) as markers of potential viral infection, as well as PCR assays including a consensus PCR and virus-specific PCR for both equine alphaherpesviruses (EHV-1; EHV-4) and gammaherpesviruses (EHV-2; EHV-5). The EHV infections were more prevalent in the TW of PAT group (P = 0.004), with the highest prevalence being for EHV-2 (P = 0.006). The EHV detection in BALs was not significantly different between groups. The EHVs detection in TW was correlated to the polymorphonuclear neutrophil (PMN) counts in the respiratory liquid but not with CCPh or ECAb. CCPh or ECAb were associated with both consensus PCR and EHV-2 and EHV-5 virus-type PCR in the BAL only. The significant detection of EHVs in the TWof PAT group in association with the PMN increased counts could lead to further investigations about their putative role in equine syndrome of tracheal inflammation [less ▲]

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See detailAttacks by a piercing-sucking insect (Myzus persicae Sultzer) or a chewing insect (Leptinotarsa decemlineata Say) on potato plants (Solanum tuberosum L.) induce differential changes in volatile compound release and oxylipin synthesis
Gosset, Virginie; Harmel, Nicolas; Goebel, Cornelia et al

in Journal of Experimental Botany (2009), 60(4), 1231-1240

Plant defensive strategies bring into play blends of compounds dependent on the type of attacker and coming from different synthesis pathways. Interest in the field is mainly focused on volatile organic ... [more ▼]

Plant defensive strategies bring into play blends of compounds dependent on the type of attacker and coming from different synthesis pathways. Interest in the field is mainly focused on volatile organic compounds (VOCs) and jasmonic acid (JA). By contrast, little is known about the oxidized polyunsaturated fatty acids (PUFAs), such as PUFA-hydroperoxides, PUFA-hydroxides, or PUFA-ketones. PUFA-hydroperoxides and their derivatives might be involved in stress response and show antimicrobial activities. Hydroperoxides are also precursors of JA and some volatile compounds. In this paper, the differential biochemical response of a plant against insects with distinct feeding behaviours is characterized not only in terms of VOC signature and JA profile but also in terms of their precursors synthesized through the lipoxygenase (LOX)-pathway at the early stage of the plant response. For this purpose, two leading pests of potato with distinct feeding behaviours were used: the Colorado Potato Beetle (Leptinotarsa decemlineata Say), a chewing herbivore, and the Green Peach Aphid (Myzus persicae Sulzer), a piercing-sucking insect. The volatile signatures identified clearly differ in function with the feeding behaviour of the attacker and the aphid, which causes the smaller damages, triggers the emission of a higher number of volatiles. In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack. Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined. Finally, the role of the insect itself on the plant response after insect infestation was highlighted. [less ▲]

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See detailOnderzoeksdesigns and statistiek
Rasier, Laurent ULg

Learning material (2009)

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See detailReconciling opposing views on carbon cycling in the coastal ocean: continental shelves as sinks and near-shore ecosystems as sources of atmospheric CO2
Chen, C. T. A.; Borges, Alberto ULg

in Deep-Sea Research Part II, Topical Studies in Oceanography (2009), 56(8-10), 578-590

Despite their moderately-sized surface area, continental marginal seas play a significant role in the biogeochemical cycles of carbon, as they receive huge amounts of upwelled and riverine inputs of ... [more ▼]

Despite their moderately-sized surface area, continental marginal seas play a significant role in the biogeochemical cycles of carbon, as they receive huge amounts of upwelled and riverine inputs of carbon and nutrients, sustaining a disproportionate large biological activity compared to their relative surface area. A synthesis of worldwide measurements of the partial pressure of CO2 (pCO2) indicates that most open shelves in the temperate and high latitude regions are under-saturated with respect to atmospheric CO2 during all seasons, although the low latitude shelves seem to be over-saturated. Most inner estuaries and near-shore coastal areas on the other hand are over-saturated with respect to atmospheric CO2. The scaling of air-sea CO2 fluxes based on pCO2 measurements and carbon mass balance calculations indicate that the continental shelves absorb atmospheric CO2 ranging between 0.33 to 0.36 Pg C yr-1 that corresponds to an additional sink of 27% to ~30% of the CO2 uptake by the open oceans based on the most recent pCO2 climatology (Takahashi et al., 2008; Deep-Sea Research II, this issue). Inner estuaries, salt marshes and mangroves emit up to 0.50 Pg C yr-1, although these estimates are prone to large uncertainty due to poorly constrained ecosystem surface area estimates. Nevertheless, the view of continental shelves as sinks and near-shore ecosystems as sources of atmospheric CO2 allows reconciling long-lived opposing views on carbon cycling in the coastal ocean. [less ▲]

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See detailINPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse
Jacoby, Monique; Cox, James J.; Gayral, Stéphanie et al

in Nature Genetics (2009), 41

The primary cilium is an antenna-like structure that protrudes from the cell surface of quiescent/differentiated cells and participates in extracellular signal processing1–3. Here, we report that mice ... [more ▼]

The primary cilium is an antenna-like structure that protrudes from the cell surface of quiescent/differentiated cells and participates in extracellular signal processing1–3. Here, we report that mice deficient for the lipid 5-phosphatase Inpp5e develop a multiorgan disorder associated with structural defects of the primary cilium. In ciliated mouse embryonic fibroblasts, Inpp5e is concentrated in the axoneme of the primary cilium. Inpp5e inactivation did not impair ciliary assembly but altered the stability of pre-established cilia after serum addition. Blocking phosphoinositide 3-kinase (PI3K) activity or ciliary platelet-derived growth factor receptor a (PDGFRa) restored ciliary stability. In human INPP5E, we identified a mutation affecting INPP5E ciliary localization and cilium stability in a family with MORM syndrome, a condition related to Bardet-Biedl syndrome. Together, our results show that INPP5E plays an essential role in the primary cilium by controlling ciliary growth factor and PI3K signaling and stability, and highlight the consequences of INPP5E dysfunction. [less ▲]

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See detailUse of cDNA-AFLP to study the defence-related gene expression in bananas (Musa spp.), inoculated with Colletotrichum musae responsible of crown rot
Lassois, Ludivine ULg; de Lapeyre de Bellaire, Luc; Jijakli, Haissam ULg

Conference (2009)

Crown rot disease of bananas is widespread in producing countries and is considered as the most important post-harvest disease of exported bananas. Variations of susceptibility to the disease have been ... [more ▼]

Crown rot disease of bananas is widespread in producing countries and is considered as the most important post-harvest disease of exported bananas. Variations of susceptibility to the disease have been noted between bananas but the origins still unknown. The biological responses of the fruit, including physiological change and disease susceptibility are controlled and regulated by gene expression. One way to understanding the reactions involved in variation of banana susceptibility to the disease in relation to their physiological state, is to study the expression of genes involved in these processes. To this purpose, crown sample previously inoculated with C. musae and showing 2 levels of susceptibility (very high and very low) were collected to be compared. Crown sample of each susceptibility level was collected at two different maturity stages: at harvest and 13 days after harvest (3 days after ripening). Collected crowns were immediately freeze-dried, an original method to conserve gene expression. cDNA-AFLP was applied on these 4 cell populations in order to highlight the differential transcription of genes whose function is "a priori" unknown. The cDNA-AFLP result was confirmed using quantitative real-time reverse transcription PCR. Various defence-related genes were identified and will be presented. [less ▲]

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See detailMethodological issues in primary prevention trials for neurodegenerative dementia.
Andrieu, Sandrine; Coley, Nicola; Aisen, Paul et al

in Journal of Alzheimer's Disease [=JAD] (2009), 16(2), 235-70

The prevention of neurodegenerative dementias, such as Alzheimer's disease, is a public health priority. Due to the large numbers of affected patients, even interventions bringing about a relatively small ... [more ▼]

The prevention of neurodegenerative dementias, such as Alzheimer's disease, is a public health priority. Due to the large numbers of affected patients, even interventions bringing about a relatively small delay in disease onset could have large public health effects. Randomized controlled trials (RCTs) are required to demonstrate the effectiveness of preventive interventions, but such trials raise specific methodological questions because they are new in the field of neurodegenerative diseases, and require large numbers of elderly subjects and lengthy follow-up periods. We performed a literature search to identify primary prevention RCTs for neurodegenerative dementia. The methodology of the trials was summarized and discussed during two expert meetings. Overall, 39 trials were identified that assessed dementia incidence or cognitive decline as a primary or secondary study outcome. Age was the most common selection criteria for target populations. Follow-up periods ranged from one month to nine years and were longest in studies measuring dementia incidence as an outcome. Results of RCTs have so far been generally negative and conflicting with those of observational studies, perhaps due to methodological issues. Future trials must therefore carefully consider the target population, outcomes and duration of follow-up to be used, and should assess the problem of attrition. [less ▲]

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See detailInfluence of drying temperature on functional properties of wet-milled starch granules
Malumba Kamba, Paul ULg; Massaux, Carine; Deroanne, Claude et al

in Carbohydrate Polymers (2009), 75

Relationships between swelling capacities, pasting properties, rotational flow behaviour and textural properties of hydro-thermally heated wet-milled starch granules from corn dried between 60 and 130°C ... [more ▼]

Relationships between swelling capacities, pasting properties, rotational flow behaviour and textural properties of hydro-thermally heated wet-milled starch granules from corn dried between 60 and 130°C were investigated. High-drying temperatures applied during the corn drying process conferred to the wet-milled starch granules (WSG) such a rigidity which reduced their swelling capacities, their water binding capacities and their water solubility index after gelatinization. These granules changes affected their pasting characteristics, their flow behaviour and several textural parameters of gel formed from the wet-milled starch granule after gelatinization. The rigidity of granules was a major factor determining the formation of either starch pastes or gels. [less ▲]

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See detailAgronomical and molecular factors influencing bananas (Musa acuminata, AAA, cv ‘Grande-Naine’) susceptibility to crown rot disease
Lassois, Ludivine ULg

Doctoral thesis (2009)

Crown rot affects export bananas in all producing countries and is considered to be one of the main export banana post-harvest disease. Variations are observed in the expression of crown rot symptoms. An ... [more ▼]

Crown rot affects export bananas in all producing countries and is considered to be one of the main export banana post-harvest disease. Variations are observed in the expression of crown rot symptoms. An original approach of the disease is proposed and consists on presenting the fruit quality potential at harvest as a key factor in crown rot development. This potential develops during growth of bananas in the field and depends on a physiological and a parasitical component. The physiological component refers here to the level of fruit susceptibility to crown rot and reflects the physiological state of the fruit. The aim of this study was to clarify the role of the fruit physiological component at harvest in the post-harvest crown rot development. It appears that the fruit physiological component at harvest greatly influence the postharvest disease development and thus the fruit susceptibility. Seasonal variations in disease severity were shown in two production area and are related to a variation of the fruit physiological component. In Guadeloupian conditions, the internal necrotic surface of the crown was nearly multiplied by 4 during 11 successive weeks. Two pre-harvest factors that could influence the fruit physiological component by modifying their susceptibility to crown rot, were identified: (i) hand position on the bunch and (ii) source-sink ratio of the banana plant (hand considered as sink and leaves as source). It was shown that within a bunch, there is a gradient of susceptibility to crown rot (r= -0.95), the hands initiated first (the upper ones) being more susceptible than those initiated last (the lower ones). These results also confirmed that source-sink ratio changes have a significant effect on fruit morphology and demonstrated that there is also an effect on fruit susceptibility to crown rot disease. When the sink is decreased by artificial removal of many hands, the level of fruit susceptibility to crown rot decreases. However, the molecular mechanisms underlying these quantitative host-pathogen relationships were still unknown. A study was designed to compare gene expression, by cDNA-AFLP, between crowns of bananas showing a high susceptibility (S+) and crowns of bananas showing a low susceptibility (S-) to Colletotrichum musae responsible for crown rot disease. This comparison was performed at two situation time: (i) between crowns (S+ and S-) collected one hour before infection and (ii) between crowns (S+ and S-) collected 13 days after infection. Genes implied in signaling pathway and proteolytic machinery were identified. It also appears that a cellulose synthase, a CAF1 gene, 2 glycolipid transfer protein and a dopamine-β-monooxygenase were differently expressed between bananas showing different levels of susceptibility. This is the first study of the characterization of the banana physiological component at harvest which influences the crown rot post-harvest disease development. In addition, to our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and different susceptibility levels. [less ▲]

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See detailGeneralized Cloaking and Optical Polyjuice
Nicolet, A.; Zolla, F.; Geuzaine, Christophe ULg

in Proceedings of the 8th International Symposium on Electric and Magnetic Fields (EMF'2009) (2009)

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See detailMagnetic susceptibility evolution and sedimentary environments on carbonate platform sediments and atolls, comparison of the Frasnian from Belgium and Alberta, Canada
Da Silva, Anne-Christine ULg; Potma, Ken; Weissenberger, John A. W. et al

in Sedimentary Geology (2009), 214

Magnetic susceptibility (MS) measurements on carbonate rocks are considered as a proxy for impurities delivered to the carbonate environments. In the absence of strong climatic or tectonic variations ... [more ▼]

Magnetic susceptibility (MS) measurements on carbonate rocks are considered as a proxy for impurities delivered to the carbonate environments. In the absence of strong climatic or tectonic variations, bulk MS values have been linked to sea level variations, because sea-level fall increases clastic supply and therefore increases magnetic mineral deposition. In this paper we explore the relationship between the average magnitude of bulk MS, with shallowing-up sequences and facies evolution in different Devonian carbonate complexes. Similarities and differences between these parameters have been scrutinized in carbonate attached platform and detached platforms (mounds and/or atolls) from Belgium and Canada. In the carbonate attached platforms from Belgium and Canada, the MS patterns are directly related to depositional environment. Mean MS values increase from the most distal towards the most proximal facies and towards the top of the majority of fourth-order shallowing-up sequences. These trends are in agreement with theoretical background (MS increases with regression). In the Belgian detached platform, the average MS pattern generally shows an opposite behaviour of that observed in the attached carbonate platforms. Average MS decreases towards the most proximal facies and towards the top of a majority of the fourth-order shallowing-up sequences. This behaviour can be explained by the influence of sedimentary rate and water agitation during deposition. A high sedimentary rate will dilute the magnetic minerals in the atoll facies and the highwater agitation during deposition may be expected to have prevented the deposition of the magnetic grains. So, the combination of these two effects will result in the observed low values in the atoll crown and lagoonal facies. In the Canadian detached platform, MS is mainly negative. This means that the limestones are very pure. The technique does not appear to be appropriate in these rocks. The variations of average MS behaviour by platform type can imply difficulties in correlating carbonates from different settings. A comparison of time equivalent mound and platform deposits shows that after an important regressive surface, the MS values are increasing for the platform deposits and decreasing for the mound. So MS evolution can be in complete opposition (caused by highly different sedimentary rates) in different depositional settings. The MS signal preserved in carbonate rocks is probably mainly related to 1) varying clastic supplies; 2) varying carbonate accumulation rates (dilution of the magnetic minerals by high carbonate production) and 3) potentially diagenesis. [less ▲]

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See detail"Metaboliquement obeses" sans exces de poids: un phenotype interpellant.
Beck, Emmanuel ULg; Scheen, André ULg

in Revue Médicale de Liège (2009), 64(1), 14-22

Obesity, especially abdominal obesity, is the main risk factor of metabolic syndrome. However, there are obviously non obese individuals who are metabolically abnormal and therefore exposed to an ... [more ▼]

Obesity, especially abdominal obesity, is the main risk factor of metabolic syndrome. However, there are obviously non obese individuals who are metabolically abnormal and therefore exposed to an increased risk of cardiovascular complications. Unfortunately, those persons fail to be detected because of a falsely reassuring body weight. The present paper aims at better understanding the etiopathogenesis and pathophysiology of this particular phenotype, at evaluating its potential clinical consequences and at describing the main principles of its therapeutic management. [less ▲]

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