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See detailClose-packed array of gold nanoparticles and Sum Frequency Generation spectroscopy in total internal reflection: A platform for studying biomolecules and biosensors
Tourillon, Gérard; Dreesen, Laurent ULg; Volcke, Cédric et al

in Journal of Materials Science (2009), 44(24), 6805-6810

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy ... [more ▼]

An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the Sum Frequency Generation spectroscopy performed in the Total Internal Reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers. [less ▲]

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See detailIntroduction: Jacqmin dans ses poèmes
Demoulin, Laurent ULg

in Textyles : Revue des Lettres Belges de Langue Française (2009), 35

Présentation de ce numéro, qui est la première monographie universitaire consacrée au poète liégeois François Jacqmin (1929-1992).

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See detailExperimental and numerical investigations of dike-break induced flows
Roger, Sebastian; Dewals, Benjamin ULg; Erpicum, Sébastien ULg et al

in Journal of Hydraulic Research (2009), 47(3), 349-359

Experimental model data are compared with numerical computations of dike-break induced flows, focusing on the final steady state. An idealised scale model was designed reproducing the specific boundary ... [more ▼]

Experimental model data are compared with numerical computations of dike-break induced flows, focusing on the final steady state. An idealised scale model was designed reproducing the specific boundary conditions of dike breaks. Discharges, water levels and depth profiles of horizontal velocities were recorded and validated by numerical modeling. The latter was performed by two different models solving the two-dimensional depth-averaged shallow water equations, namely a total variation diminishing Runge-Kutta dis¬continuous Galerkin finite element method, and a finite volume scheme involving a flux vector splitting ap¬proach. The results confirmed convergence and general applicability of both methods for dike-break prob¬lems. As regards their accuracy, the basic flow pattern was satisfactorily reproduced yet with differences compared to the measurements. Hence, additional simulations by a finite volume model were performed con¬sidering various turbulence closures, wall-roughnesses as well as non-uniform Boussinesq coefficients. [less ▲]

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See detailPreliminary characterization of jejunocyte and colonocyte cell lines isolated by enzymatic digestion from adult and young cattle.
Loret, Suzanne; Rusu, Dorina; Moualij, Benaissa El et al

in Research in Veterinary Science (2009), 87(1), 123-32

In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]

In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages for non-transfected cells), (2) the detection of both the large T transcript and the large T antigen in transformed cells. Possible contamination and progressive substitution of bovine primocultures by non-bovine lineages available in the laboratory was excluded, as the transformed cells presented a bovine typical karyotype. Most transfected cells kept an epithelial morphology after transformation. They also maintained the expression of FABP and enterocyte specific enzymes (brush-border associated maltase and IAP). However, levels of specific activity of these enzymes were low, suggesting that cell differentiation is not completely achieved under the applied culture conditions. [less ▲]

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See detailNeed for high-standard translation methodology for the dissemination of guidelines.
Henrotin, Yves ULg

in Osteoarthritis and Cartilage (2009), 17(12), 1536-8

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See detailBetaine Adducts of N-Heterocyclic Carbenes: Synthesis, Properties, and Reactivity
Delaude, Lionel ULg

in European Journal Of Inorganic Chemistry (2009), (13), 1681-1699

N-Heterocyclic carbenes (NHCs) form stable zwitterionic adducts with a range of heteroallenes, ketenes, and allenes. Although the first representatives of this class of inner salts were first investigated ... [more ▼]

N-Heterocyclic carbenes (NHCs) form stable zwitterionic adducts with a range of heteroallenes, ketenes, and allenes. Although the first representatives of this class of inner salts were first investigated as far back as the 1960s, they have enjoyed a sustained interest from the chemical community over the years. Depending on the nature of their anionic moiety, NHC betaines display a very broad palette of reactivities and have found applications in various fields of organic synthesis and catalysis. In this Microreview, the synthesis, properties, and reactivity of NHC betaines are surveyed. The NHCs under consideration include ylidenes derived from imidazole, benzimidazole, imidazoline, thiazole, or triazole, and the heteroallenes investigated so far are carbon dioxide, carbon disulfide, isocyanates, isothiocyanates, and their selenium analogues. A historical background is provided for each type of adduct under consideration, but emphasis is placed mainly on developments that have appeared in the literature within the past few years. ((c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009) [less ▲]

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See detailGrain boundary effects in bulk colossal magneto resistive (CMR) manganites and manganite/insulator composites: electrical and magnetic properties
Vanderbemden, Philippe ULg; Vertruyen, Bénédicte ULg; Ausloos, Marcel ULg et al

in Journal of Optoelectronics and Advanced Materials [= JOAM] (2009), 11(9), 1115-1121

In the first part of this paper, we discuss the effects of grain boundaries on the properties of bulk colossal magnetoresistive (CMR) manganites We compare the electrical resistivity and AC magnetic ... [more ▼]

In the first part of this paper, we discuss the effects of grain boundaries on the properties of bulk colossal magnetoresistive (CMR) manganites We compare the electrical resistivity and AC magnetic susceptibility of perovskite La-Ca-Mn-O samples with the same nominal stoichiometry but differing in their microstructure (i) a single grain sample, (ii) a sample containing two grains and (iii) a polycrystalline sample Emphasis is placed on information that can be deduced from the measurements in each case In the second part of the paper, we report the data measured on composite samples containing a CMR phase (La-Ca-Mn-O) and an insulating phase (Mn3O4) The results are discussed in the framework of percolation theory We show how the grain boundaries affect the electrical properties of these materials, and we highlight the crucial role of geometric (demagnetization) effects on the resistance vs magnetic field measurements [less ▲]

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See detailSocial Enterprise: the Shaping of a New Concept in a Comparative Regional Perspective
Defourny, Jacques ULg; Nyssens, Marthe

in Defourny, Jacques; Develtere, Patrick; Fonteneau, Bénédicte (Eds.) et al The Worldwide Making of the Social Economy. Innovations and Changes (2009)

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See detailComparative analysis of painting recipes: a new contribution to the study of the texts of the Strasbourg family
Neven, Sylvie ULg

in HERMENS, Erma; TOWNSEND, Joyce (Eds.) Sources and Serendipity. Testimonies of Artists’ Practice (2009)

For many years, the Strasbourg Manuscript was believed to be the oldest German-language source for the study of Northern European painting techniques. Unfortunately, the manuscript was lost in the 1870 ... [more ▼]

For many years, the Strasbourg Manuscript was believed to be the oldest German-language source for the study of Northern European painting techniques. Unfortunately, the manuscript was lost in the 1870 fire at the Strasbourg Library, and the text only survives in a 19th-century transcription made for Eastlake (1847). Studies such as those of Ploss and more recently those of Oltrogge have already highlighted the existence of texts with very similar contents, grouping them under the name of the ‘Strasbourg family’. Since these studies, a clearly defined corpus of manuscripts has been established as belonging to this family. This paper shows the different degrees of connection between the texts and presents some suggestions concerning their compilation process. The possibility of collating different versions of an identical text and cross-checking recipes can also be exploited to learn more about the ways in which recipe books were compiled, and to provide information concerning their use and function. [less ▲]

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See detailLes codes La Charte. Droit public et administratif
Bourgeois, Marc ULg; Behrendt, Christian ULg; Lewalle, Paul ULg et al

Book published by La Charte (2009)

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See detailDielectric Multilayer Films Fabricated by Magnetron Sputtering: How Far Can the Iridescence Be Tuned?
Deparis, Olivier; Rassart, Marie; Vandenbem, Cedric et al

in Plasma Processes and Polymers (2009), 6

Periodic dielectric multilayer structures can generate interferential colours from optically transparent basic materials. Their iridescence property, i.e. the change of colour with the illumination or ... [more ▼]

Periodic dielectric multilayer structures can generate interferential colours from optically transparent basic materials. Their iridescence property, i.e. the change of colour with the illumination or viewing angle, is exploited in the industry to produce structurally coloured coatings and paints. Magnetron sputtering is an efficient technique for producing multilayer films owing to its ability to deposit films on large surfaces with excellent uniformity and reproducibility in both the film thickness and composition. Based on a theoretical model of the optical response, we investigated the iridescence tuning range of the technologically important SiO2/TiO2 material system. Radically different iridescent behaviours were predicted theoretically and demonstrated experimentally by selecting appropriate combinations of period and layer thickness ratio. [less ▲]

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See detailFaire et ne pas faire dans le cadre d'une politique intégrale et intégrée sur les drogues.
De Ruyver, Brice; Lemaître, André ULg; Born, Michel ULg et al

Book published by Academia Press (2009)

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See detailVarietal effects of barley carbohydrate composition on digestibility, fermentability and microbial ecophysiology in an in vitro model of the pig gastrointestinal tract.
Bindelle, Jérôme ULg; Pieper, Robert; Rossnagel, Brian et al

in Journal of Animal Science (2009), 87(E-Suppl. 3), 93

Carbohydrate (CHO) composition can vary markedly between barley varieties. Their influence on digestibility, intestinal fermentation and microbiota in pigs was studied in vitro. Ten hulless (HLB) and 6 ... [more ▼]

Carbohydrate (CHO) composition can vary markedly between barley varieties. Their influence on digestibility, intestinal fermentation and microbiota in pigs was studied in vitro. Ten hulless (HLB) and 6 hulled barleys (HB) differing in B-glucan, non-starch polysaccharides (NSP), starch content, and amylose/amylopectin ratio, were hydrolyzed enzymatically and subsequently fermented for 72h. CHO fermentation kinetics were modeled; microbial composition and short-chain fatty acid (SCFA) production were analyzed. In HLB, in vitro DM digestibility was positively correlated to starch and amylopectin content and CP digestibility to amylopectin (P<0.05), whereas both were negatively correlated to insoluble NSP (P<0.05). Rate of fermentation was different (P<0.01) between barley types but not correlated to the CHO composition. However, high B-glucan contents induced faster fermentation (P<0.05, HLB; P<0.10, HB). SCFA molar ratios after fermentation of HLB were higher in propionate and branchedchain fatty acids and lower in acetate compared to HB (P<0.01). With HLB, amylose content was positively correlated to butyrate production and negatively to propionate, which was positively correlated to soluble NSP content (P<0.01). In HB, no correlation between SCFA production and the carbohydrate composition was found. TRFLP analysis revealed that Bacteroides and members of Clostridium cluster XIVa were differentially affected in HLB compared to HB as well as by the type and source of CHO. Microbial profiles were also correlated (P<0.05) to SCFA and fermentation parameters but response differed significantly between HB and HLB. The strongest correlation between CHO structure, microbial abundance and fermentation parameters was evident in HLB. Hulless barleys may offer the greatest opportunity to improve gut health in pigs. [less ▲]

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See detailThe impact of auditory selective attention on verbal short-term memory and vocabulary development
Majerus, Steve ULg; Heiligenstein, Lucie; Gautherot, N. et al

in Journal of Experimental Child Psychology (2009), 103

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See detailPsychostatistique descriptive et inférentielle Partim 1 - Théorie
Quertemont, Etienne ULg

Learning material (2009)

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See detailAn Extra-trees-based Automatic Target Recognition Algorithm
Pisane, Jonathan ULg; Marée, Raphaël ULg; Ries, Philippe ULg et al

in To appear in Proc. International Radar Conference (2009)

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See detailDe la codicologie à l'archéologie du savoir. Le cas particulier du carnet de notes de l'historien égyptien al-Maqrīzī (m. 845/1442)
Bauden, Frédéric ULg

in Archives et Bibliothèques de Belgique = Archief en Bibliotheekwezen in Belgie (2009), 87

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See detailPoésie et subversion. Rimbaud mauvaises manières.
Saint-Amand, Denis ULg

in Bernadet, Arnaud (Ed.) Rimbaud, l'invisible et l'inouï (2009)

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