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See detailVarietal effects of barley carbohydrate composition on digestibility, fermentability and microbial ecophysiology in an in vitro model of the pig gastrointestinal tract.
Bindelle, Jérôme ULg; Pieper, Robert; Rossnagel, Brian et al

in Journal of Animal Science (2009), 87(E-Suppl. 3), 93

Carbohydrate (CHO) composition can vary markedly between barley varieties. Their influence on digestibility, intestinal fermentation and microbiota in pigs was studied in vitro. Ten hulless (HLB) and 6 ... [more ▼]

Carbohydrate (CHO) composition can vary markedly between barley varieties. Their influence on digestibility, intestinal fermentation and microbiota in pigs was studied in vitro. Ten hulless (HLB) and 6 hulled barleys (HB) differing in B-glucan, non-starch polysaccharides (NSP), starch content, and amylose/amylopectin ratio, were hydrolyzed enzymatically and subsequently fermented for 72h. CHO fermentation kinetics were modeled; microbial composition and short-chain fatty acid (SCFA) production were analyzed. In HLB, in vitro DM digestibility was positively correlated to starch and amylopectin content and CP digestibility to amylopectin (P<0.05), whereas both were negatively correlated to insoluble NSP (P<0.05). Rate of fermentation was different (P<0.01) between barley types but not correlated to the CHO composition. However, high B-glucan contents induced faster fermentation (P<0.05, HLB; P<0.10, HB). SCFA molar ratios after fermentation of HLB were higher in propionate and branchedchain fatty acids and lower in acetate compared to HB (P<0.01). With HLB, amylose content was positively correlated to butyrate production and negatively to propionate, which was positively correlated to soluble NSP content (P<0.01). In HB, no correlation between SCFA production and the carbohydrate composition was found. TRFLP analysis revealed that Bacteroides and members of Clostridium cluster XIVa were differentially affected in HLB compared to HB as well as by the type and source of CHO. Microbial profiles were also correlated (P<0.05) to SCFA and fermentation parameters but response differed significantly between HB and HLB. The strongest correlation between CHO structure, microbial abundance and fermentation parameters was evident in HLB. Hulless barleys may offer the greatest opportunity to improve gut health in pigs. [less ▲]

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See detailEvaluation of the contamination of North Sea Porpoises (Phocoena phocoena) by endocrine disrupting chemicals using CG-MS and in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Remacle, Anne-Sophie ULg et al

Poster (2009)

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be ... [more ▼]

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be very slowly degraded, decreasing the environmental quality and causing ecological risks. Marine mammals inhabiting polluted environments accumulate high quantities of these chemicals, and are good indicators of marine pollution Thirteen major organochloride pollutants (known from literature to contaminate north sea porpoise) were chosen in this study – o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. The thirteen chemicals were tested individually and in mixtures by the use of report gene expression assays. The MCF7-ERE cells used in the assays were originated from MCF7 human mammary tumor cells transfected with the ERE-luciferase reporter system. Preliminary results suggest that o,p’-DDD; p,p’-DDD; o,p’-DDT; HCB; β-HCH; δ-HCH; PCB 138 and PCB 180 are able to stimulate the luciferase expression of MCF7-ERE cells when they are present individually or in mixtures. No synergism was observed within mixtures. When MCF7-ERE cells were exposed to the chemicals and 17β-oestradiol simultaneously, p,p’-DDE; α-HCH; PCB 138 and PCB 180 could raise the luciferase expression in comparison to the exposition of 17β-oestradiol alone; on the other hand, o,p’-DDD; p,p’-DDT; HCB; β-HCH; γ-HCH and δ-HCH seem to inhibit the luciferase expression at low doses. In the next step, the pollutants will be searched and quantified in the blubber tissue of porpoises by use of gas chromatography-mass spectrometry. The blubber tissue samples will also have their endocrine disrupting effects characterized. This work is ongoing and It is expected a relevant level of these compounds to be found in the samples. Conversely, their endocrine disrupting effects and how they act in agonist, antagonist ways are subject that still needs to be studied. [less ▲]

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See detailA revised carbon budget for the North Sea
Paetsch, J.; Kuehn, W.; Borges, Alberto ULg et al

Conference (2009)

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See detailUn outil d'aide à la décision pour la gestion des chablis en Région wallonne.
Riguelle, Simon ULg; Jourez, Benoît ULg; Hebert, Jacques ULg

in Silva Belgica (2009), 116(5), 34-38

Depuis 1990, la forêt wallonne a été épargnée par les violentes tempêtes qui ont dévasté les massifs forestiers de l’Europe de l’Ouest ces dernières décennies (1999, 2005, 2007, 2009). Néanmoins, il ne ... [more ▼]

Depuis 1990, la forêt wallonne a été épargnée par les violentes tempêtes qui ont dévasté les massifs forestiers de l’Europe de l’Ouest ces dernières décennies (1999, 2005, 2007, 2009). Néanmoins, il ne saurait en être éternellement ainsi, c’est pourquoi les pouvoirs publics régionaux et les professionnels de la filière bois ont décidé de se préparer dès maintenant à gérer une future catastrophe de grande ampleur. Dans cette optique, le DEMNA et la FUSAGx ont développé des procédures et des outils susceptibles d’aider les gestionnaires de crise à réagir efficacement dans l’urgence et à définir la meilleure stratégie opérationnelle pour valoriser les bois chablis. [less ▲]

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See detailPierre-Augustin Guys à Constantinople: un regard méconnu sur le Levant à la fin du XVIIIe siècle
Famerie, Etienne ULg

in Droixhe, Daniel; Collart, Muriel (Eds.) L'"Esprit des journaux": un périodique européen au XVIIIe siècle (2009)

Guys' original approach and description of the contemporaneous Greek people, breaking a new ground in favour of the philhellenic movement in the end of 18th century

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See detailConditional knockout of nucleolin in DT40 cells reveals the functional redundancy of its RNA-binding domains.
Storck, Sebastien; Thiry, Marc ULg; Bouvet, Philippe

in Biology of the Cell (2009), 101(3), 153-67

BACKGROUND INFORMATION: Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a ... [more ▼]

BACKGROUND INFORMATION: Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nucleolin influences cell proliferation remained elusive so far. RESULTS: We have generated conditional nucleolin-knockout cells using the chicken B lymphocyte cell line DT40. Our results indicate that nucleolin is absolutely required for the proliferation and for the survival of these cells. Depletion of nucleolin drastically inhibits rDNA (ribosomal DNA) transcription while only slightly affecting pre-rRNA processing. This inhibition is accompanied by modifications of the shape and the structure of the nucleolus. The analysis of mutants of nucleolin, which lack two or three RNA-binding domains, shows that these domains harbour redundant functions and that nucleolin's roles in transcription, rRNA maturation and nucleolar shape can be partially uncoupled. CONCLUSIONS: The function of nucleolin in ribosomal synthesis could account for its effect on cell division and survival, but this vital role does not seem to be linked to sequence-specific RNA binding. [less ▲]

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See detailStrontium ranelate: new data on fracture prevention and mechanisms of action.
Reginster, Jean-Yves ULg; Deroisy, Rita ULg; Neuprez, Audrey et al

in Current Osteoporosis Reports (2009), 7(3), 96-102

Osteoporosis treatments need to combine an unequivocally demonstrated reduction of fractures, at various skeletal sites, long-term safety, and a user-friendly profile that optimizes therapeutic adherence ... [more ▼]

Osteoporosis treatments need to combine an unequivocally demonstrated reduction of fractures, at various skeletal sites, long-term safety, and a user-friendly profile that optimizes therapeutic adherence. Strontium ranelate is the first compound to simultaneously decrease bone resorption and stimulate bone formation. Its anti-fracture efficacy at various skeletal sites has been established for as long as 5 years through studies of the highest methodological standards. Increases in bone mineral density observed after 1 year of treatment are predictive of the long-term fracture efficacy, suggesting for the first time in osteoporosis that bone densitometry can be used as a monitoring tool. Due to a positive risk/benefit ratio, strontium ranelate is now considered as a first-line treatment in the management of osteoporosis. [less ▲]

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See detailModal testing of nonlinear vibrating structures based on a nonlinear extension of force appropriation
Peeters, Maxime ULg; Kerschen, Gaëtan ULg; Golinval, Jean-Claude ULg

in ASME International Design Engineering Technical Conferences, San Diego, 2009 (2009)

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See detailModal testing of non-linear vibrating structures
Peeters, Maxime ULg; Kerschen, Gaëtan ULg

in 11th European Conference on Spacecraft Structures, Materials and Mechanical Testing, Toulouse, 2009 (2009)

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See detailRelations between a computerized shopping task and cognitive tests in a group of persons diagnosed with schizophrenia compared with healthy controls
Laroi, Frank ULg; Canlaire, J.; Mourad, H. et al

in Journal of the International Neuropsychological Society (2009), 16

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See detailElectron detachment dissociation (EDD) pathways in oligonucleotides
Kinet, Catherine ULg; Gabelica, Valérie ULg; Balbeur, Dorothée ULg et al

in International Journal of Mass Spectrometry (2009), 283

Electron detachment dissociation (EDD) and electron photodetachment dissociation (EPD) are two novel fragmentation methods yielding radicals from negatively charged ions. With the goal of comparing EDD ... [more ▼]

Electron detachment dissociation (EDD) and electron photodetachment dissociation (EPD) are two novel fragmentation methods yielding radicals from negatively charged ions. With the goal of comparing EDD, EPD and the more traditional Collision-Induced Dissociation (CID) and Infrared Multiphoton Disscociation (IRMPD) fragmentation processes in oligonucleotides, we studied here the EDD fragmentation pathways of oligonucleotides of varying length. We chose polythymine oligonucleotides because these are the least prone to secondary structure formation, and found complete sequence coverage by EDD for up to dT20. We also found that the fragmentation pathways change with oligonucleotide length: electron detachment is a mandatory step in the fragmentation of larger sequences, while shorter oligonucleotides can also fragment via direct electronic or vibrational excitation by the electrons. This is supported by (1) the fact that continuous ejection of the charge reduced species does not totally prevent fragmentation of short oligonucleotides dT5 and dT6, (2) the fact that CID and EDD fragments are more similar for small oligonucleotides (although double resonance experiments show that they are not all issued from the same mechanisms), and (3) the fact that electron-induced dissociation (EID) of singly charged dT3 and dT4 gives similar fragments as EDD of doubly charged dT5 and dT6. Finally, the detachment efficiency as a function of the nature of the nucleobase was studied. The effect of base on electron detachment in EDD (G > T > A > C) is different than in EPD (G > A > C > T), indicating different electron loss mechanisms. [less ▲]

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See detailL'assouplissement des règles relatives à la prononciation d'une décision en matière répressive
Michiels, Olivier ULg

in Revue de Droit Pénal et de Criminologie (2009)

C'est le Code judiciaire qui énonce la marche à suivre pour la prononciation d'un jugement. L'article fait le point sur la manière dont une décision répressive doit être prononcée.

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See detailThe usefulness of operant conditioning procedures to assess long-lasting deficits following transient focal ischemia in mice.
Ferrara, André ULg; El Bejaoui, Sophie; Seyen, Sandrine ULg et al

in Behavioural Brain Research (2009), 205(2), 525-34

In this study, we examined a number of short and long-term sensorimotor, behavioural and cognitive consequences of an experimental ischemia induced by a 60-min right middle cerebral artery occlusion (MCAO ... [more ▼]

In this study, we examined a number of short and long-term sensorimotor, behavioural and cognitive consequences of an experimental ischemia induced by a 60-min right middle cerebral artery occlusion (MCAO) in 129S2 mice. During 14 days after surgery, a classical sensorimotor assessment was conducted using hanging wire test, negative geotaxis test, grip strength test, accelerated rotarod test and locomotor activity-meter. In order to provide a technique for the assessment of more resistant consequences of ischemia on fine psychomotor control, the peak procedure (a modified version of the operant fixed-interval schedule of reinforcement) was used. This procedure also helped to objectify temporal perception in mice five weeks following surgery. On several sensorimotor tests, ischemic mice showed some degree of impairment which rapidly tended to improve after stroke, a profile of results substantially consistent with previous studies. Five weeks post-surgery, ischemic mice tested with the peak procedure exhibited a moderate but yet significant temporal regulation impairment along with a reduced response rate compared to control mice. The present results suggest that the peak procedure and other derived operant schedules of reinforcement may provide useful and sensitive tools for the long-term assessment of both behavioural and cognitive aspects of the consequences of an experimental ischemia. [less ▲]

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See detailMATISSE Science Cases
Wolf, S.; Lopez, B.; Jaffe, W. et al

in Moorwood, A. (Ed.) Science with the VLT in the ELT Era (2009)

MATISSE is foreseen as a mid-infrared spectro-interferometric instrument combining the beams of up to four UTs/ATs of the Very Large Telescope Interferometer (VLTI). MATISSE will measure closure phase ... [more ▼]

MATISSE is foreseen as a mid-infrared spectro-interferometric instrument combining the beams of up to four UTs/ATs of the Very Large Telescope Interferometer (VLTI). MATISSE will measure closure phase relations and thus offer an efficient capability for image reconstruction. In addition to this, MATISSE will open 2 new observing windows at the VLTI: the L and M band in addition to the N band. Furthermore, the instrument will offer the possibility to perform simultaneous observations in separate bands. MATISSE will also provide several spectroscopic modes. In summary, MATISSE can be seen as a successor of MIDI by providing imaging capabilities in the mid-infrared domain (for a more detailed description of MATISSE see Lopez et al., these proceedings). [less ▲]

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See detailOn modal interactions in strongly nonlinear systems
Peeters, Maxime ULg; Kerschen, Gaëtan ULg; Golinval, Jean-Claude ULg

in Euromech 503, Nonlinear Normal Modes, Dimension Reduction and Localization in Vibrating Systems, Frascati, 2009 (2009)

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See detailPlasmonic Ratchet Wheels: Switching Circular Dichroism by Arranging Chiral Nanostructures
Valev, V. K.; Smisdom, N.; Silhanek, Alejandro ULg et al

in Nano Letters (2009), 9(11), 3945-3948

We demonstrate circular dichroism (CD) in the second harmonic generation (SHG) signal from chiral assemblies of G-shaped nanostructures made of gold. The arrangement of the G shapes is crucial since upon ... [more ▼]

We demonstrate circular dichroism (CD) in the second harmonic generation (SHG) signal from chiral assemblies of G-shaped nanostructures made of gold. The arrangement of the G shapes is crucial since upon reordering them the SHG-CD effect disappears. Microscopy reveals SHG "hotspots" assemblies, which originate in enantiomerically sensitive plasmon modes, having the novel property of exhibiting a chiral geometry themselves in relation with the handedness of the material. These results open new frontiers in studying chirality. [less ▲]

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See detailReptile cell cultures for nucleolar studies
Lamaye, Françoise; Thiry, Marc ULg

Poster (2009)

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See detailLe Dévonien: des mousses à l'arbre.
Gerrienne, Philippe ULg

Conference (2009)

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