References of "2009"
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See detailBody dermatitis in a donkey caused by the Psoroptes ovis
Mignon, Bernard ULg; Lekimme, Mireille ULg; Huynen, K. et al

Conference (2009)

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See detailRééducation de l'apathie dans la maladie d'Alzheimer par l'exploitation des domaines d'expertise du patient
Adam, Stéphane ULg

in Revue Francophone de Gériatrie et de Gérontologie (2009), 158

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See detailUn outil d'aide à la décision pour la gestion des chablis en Région wallonne.
Riguelle, Simon ULg; Jourez, Benoît ULg; Hebert, Jacques ULg

in Silva Belgica (2009), 116(5), 34-38

Depuis 1990, la forêt wallonne a été épargnée par les violentes tempêtes qui ont dévasté les massifs forestiers de l’Europe de l’Ouest ces dernières décennies (1999, 2005, 2007, 2009). Néanmoins, il ne ... [more ▼]

Depuis 1990, la forêt wallonne a été épargnée par les violentes tempêtes qui ont dévasté les massifs forestiers de l’Europe de l’Ouest ces dernières décennies (1999, 2005, 2007, 2009). Néanmoins, il ne saurait en être éternellement ainsi, c’est pourquoi les pouvoirs publics régionaux et les professionnels de la filière bois ont décidé de se préparer dès maintenant à gérer une future catastrophe de grande ampleur. Dans cette optique, le DEMNA et la FUSAGx ont développé des procédures et des outils susceptibles d’aider les gestionnaires de crise à réagir efficacement dans l’urgence et à définir la meilleure stratégie opérationnelle pour valoriser les bois chablis. [less ▲]

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See detailA modular approach to quality of life assessment in paediatric oncology
Fonseca, Marta; Missotten, Pierre ULg; Etienne, Anne-Marie ULg et al

in Psychology & Health (2009), 24(Supp 1), 175

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See detailLes premières plantes terrestres (Embryophytes) : origine et diversification - première partie.
Gonez, Paul ULg; Prestianni, Cyrille ULg; Strullu-Derrien, Marie-Christine et al

Conference (2009)

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See detailExperimental study of a river biofilm growth on artificial cobbles in contrasted flow conditions
Moulin, F.; Peltier, Yann ULg; Pen, C. et al

in International Workshop on Environmental Hydraulics (2009)

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See detailExploration of nonlinear shunting strategies as effective vibration absorbers
Viguié, Régis ULg; Kerschen, Gaëtan ULg; Ruzzene, M.

in Smart Str. and Mat. & Nondestructive Eval. and Health Monitoring, San Diego, 2009 (2009)

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See detailIn vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection.
Gillet, Laurent ULg; May, Janet S; Stevenson, Philip G

in Journal of General Virology (The) (2009), 90(Pt 3), 602-13

Many herpesviruses bind to heparan sulfate (HS). Murid herpesvirus-4 (MuHV-4) does so via its envelope glycoproteins gp70 and gH/gL. MuHV-4 gp150 further regulates an HS-independent interaction to make ... [more ▼]

Many herpesviruses bind to heparan sulfate (HS). Murid herpesvirus-4 (MuHV-4) does so via its envelope glycoproteins gp70 and gH/gL. MuHV-4 gp150 further regulates an HS-independent interaction to make that HS-dependent too. Cell binding by MuHV-4 virions is consequently strongly HS-dependent. Gp70 and gH/gL show some in vitro redundancy: an antibody-mediated blockade of HS binding by one is well tolerated, whereas a blockade of both severely impairs infection. In order to understand the importance of HS binding for MuHV-4 in vivo, we generated mutants lacking both gL and gp70. As expected, gL(-)gp70(-) MuHV-4 showed very poor cell binding. It infected mice at high dose but not at low dose, indicating defective host entry. But once entry occurred, host colonization, which for MuHV-4 is relatively independent of the infection dose, was remarkably normal. The gL(-)gp70(-) entry deficit was much greater than that of gL(-) or gp70(-) single knockouts. And gp150 disruption, which allows HS-independent cell binding, largely rescued the gL(-)gp70(-) cell binding and host entry deficits. Thus, it appeared that MuHV-4 HS binding is important in vivo, principally for efficient host entry. [less ▲]

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See detailOn modal interactions in strongly nonlinear systems
Peeters, Maxime ULg; Kerschen, Gaëtan ULg; Golinval, Jean-Claude ULg

in Euromech 503, Nonlinear Normal Modes, Dimension Reduction and Localization in Vibrating Systems, Frascati, 2009 (2009)

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See detailBehavioral Assessment in Patients with Disorders of Consciousness: Gold Standard or Fool’s Gold?
Schnakers, Caroline ULg; Giacino, Joseph; Rodriguez-Moreno et al

in Progress in Brain Research (2009), 177

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See detailUne nouvelle voie pour le matérialisme politique. Remarques sur l’anthropologie négative de Marx et l’anthropologie positive de Proudhon
Frère, Bruno ULg

in Revue du Mouvement Anti-Utilitariste dans les Sciences Sociales (2009), 34

This article aims to show that in the socialist tradition, alongside Marx, there is a position as materialist – or perhaps more so – that it would be valuable to combine with his powerful critique of ... [more ▼]

This article aims to show that in the socialist tradition, alongside Marx, there is a position as materialist – or perhaps more so – that it would be valuable to combine with his powerful critique of capitalism. This perspective is that of Proudhon. According to the author, there are possibilities of emancipation through work that are not exclusively conditioned by the “Great Day”, typical of Marxist rhetoric, and which emerge here and now thanks to this perspective. Of course Prouhon’s work is philosophically and economically less powerful than that of Marx. However, his work is interesting in that he enables us to see in the “working” man something other than a stubborn animal, de-subjectivized by capitalism and existing only as a means of production. This is why, if indeed this is anthropology, it may be called positive anthropology. By continuously and almost exclusively favoring the notion of alienation, Marx runs the risk of allowing a purely negative anthropology to emerge in his work. This even today feeds the critical theory of certain authors who see in the modern man an alienated and brainless consumer who must be stripped of his illusions. [less ▲]

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See detailActivation of equine neutrophils by phorbol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine induces a different response in reactive oxygen species production and release of active myeloperoxidase.
Franck, Thierry ULg; Kohnen, Stéphane; de la Rebière de Pouyade, Geoffroy ULg et al

in Veterinary Immunology and Immunopathology (2009)

Neutrophil (PMN) contribution to the acute inflammatory processes may lead to an excessive generation of reactive oxygen metabolites species (ROS) and secretion of granule enzymes. We compared the effects ... [more ▼]

Neutrophil (PMN) contribution to the acute inflammatory processes may lead to an excessive generation of reactive oxygen metabolites species (ROS) and secretion of granule enzymes. We compared the effects of either phorbol myristate acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanine (fMLP) in combination with a pre-treatment by cytochalasin B (CB) on the production of ROS and the release of total and active myeloperoxidase (MPO) by isolated equine PMNs. The ROS production was assessed by lucigenin dependent chemiluminescence (CL) and ethylene release by alpha-keto-gamma-methylthiobutyric acid (KMB) oxidation. In the supernatant of activated PMNs, total equine MPO was measured by ELISA and active MPO by the SIEFED (Specific Immunologic Extraction Followed by Enzymatic Detection) technique that allows for the study of the interaction of a compound directly with the enzyme. The stimulation of PMNs with CB-fMLP only modestly increased the release of MPO, but more than 70% of released MPO was active. PMA stimulation markedly increased the production of ROS and release of MPO, but more than 95% of released MPO was inactive. When PMNs were pre-incubated with superoxide dismutase (SOD) prior to PMA activation, the lucigenin enhanced CL, which is linked to the superoxide anion (O(2)(-)) production, was much more decreased than KMB oxidation, linked to the hydroxyl-like radical production. The addition of SOD prior to the activation of PMNs by PMA also limited the loss of the activity of released MPO. These results confirm the key role of O(2)(-) generation in the ROS cascade in PMN and reveal its critical role on MPO inactivation. [less ▲]

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See detailLes papyrus iatromagiques
de Haro Sanchez, Magali ULg

Textual, factual or bibliographical database (2009)

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See detailAlcohol consumption and the prevalence of metabolic syndrome : a meta-analysis of observational studies
Alkerwi, A; Boutsen, M; Vaillant, M et al

Poster (2009)

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See detailMethod for co-purification of equine neutrophil elastase and myeloperoxidase from a limited blood volume.
de la Rebière de Pouyade, Geoffroy ULg; Serteyn, Didier ULg; Deby, Ginette ULg et al

in Research in Veterinary Science (2009)

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities ... [more ▼]

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities. We describe the isolation of both enzymes from a same limited blood volume. Both MPO and elastase were extracted by crushing PMN isolated by centrifugation on a percoll-gradient from a 460ml blood collection. MPO and elastase were separated by an ionic exchange chromatography phase and further purified by gel filtration chromatography on Superdex 200 and 75, respectively. Enzymes were identified in the collected fractions by specific enzymatic assays. The final purity was verified by electrophoresis. Specific activity was improved to 19.92 and 34.3x for elastase (final yield: 340mug) and MPO (final yield: 130mug), respectively, during the procedure. Results show the possibility of isolating both enzymes from the same blood sample with a sufficient yield and purity for future studies on their implication and interaction during inflammatory diseases. [less ▲]

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See detailValidation of methods for the detection of new emerging pathogenic Escherichia coli
Verstraete, K; De Reu, K; Robyn, J et al

Book published by Brussels : Belgian Science Policy (2009)

Enterohaemorrhagic Escherichia coli (EHEC) are shigatoxin producing E. coli (STEC) that can cause serious disease to humans. These food-borne pathogens belong to the fifth most common zoonoses in Belgium ... [more ▼]

Enterohaemorrhagic Escherichia coli (EHEC) are shigatoxin producing E. coli (STEC) that can cause serious disease to humans. These food-borne pathogens belong to the fifth most common zoonoses in Belgium, but due to their severe clinical symptoms in humans they are highly dreaded. They can cause a range of disease symptoms ranging from asymptomatically carriage over various diarrhoea symptoms to the life-threatening HUS (haemolytic uremic syndrome). Cattle are the main reservoir and infection of humans occurs through contact with faecal excretion material and consumption of contaminated food or water. A broad variety of serotypes is able to cause human infections, but the principal serotypes are O26, O103, O111, O145 and O157. These strains are denoted as new emerging pathogens by the WHO. The group of sorbitol non-fermenting (s-) O157:H7 strains are examined the most, because an ISO-method is available. For sorbitol fermenting (s+) O157 strains as well as for non-O157 STEC strains recently a new isolation method was developed in the Belspo project SD/AF/06A (Possé et al. 2008a). The aim of the project was the optimization and the validation of the above-mentioned detection and isolation method for STEC in different matrices. In the first place immunomagnetic separation (IMS) was evaluated for the optimization of the STEC isolation method for cattle faeces (Ghent University, UGent). Second, molecular characterization of STEC strains was performed using a newly designed 33-mPCR as an alternative tool (University of Antwerp, VIB) and pulsed field gel electrophoresis (PFGE) (Institute for Agricultural and Fisheries Research, ILVO). Also a smaller derived multiplex PCR (9-mPCR) was designed (VIB) and optimized for the screening of samples (ILVO). The third goal was the evaluation of different approaches for STEC isolation from human faecal samples (Universitair Ziekenhuis Brussel, UZ). Finally the STEC detection and isolation method was validated by an in-house and an interlaboratory study which was based on the ISO 16140 guideline for the validation of alternative methods (University of Liège; UGent; ILVO). For the optimization of the STEC isolation protocol for cattle faeces and the evaluation of the effect of IMS, cattle faecal samples were artificially inoculated with various numbers of STEC (10-100 and 100-1000 cfu/25g faeces) and isolated using the isolation protocol with 6h or 24h of enrichment followed by IMS and plating or direct plating on selective agars. Two types of IMS beads (Dynabeads and Captivate beads) were tested. Results showed that IMS (any of the two types of beads) had a highly positive effect on the isolation of serotype O157 (s- and s+), whereas only a small or even a negative effect for non-O157 serotypes was found. This was largely clarified by results on pure broth suspensions of STEC, showing that high percentages were recovered from the IMS beads used in suspensions with the serotypes O157 (s- and s+), O26 and O103, but lower percentages were recovered for O111 and O145. Non-O157 STEC were often already efficiently isolated from faeces using only direct plating, whereas O157 (s- and s+) STEC were not. For the enrichment time, 24h generally gave higher isolation efficiencies than 6h. Finally for serotypes O157 (s- and s+), O26 and O103, a level of 10-100 cfu/25g was reliably detected, whereas for serotypes O111 and O145 only 100-1000 cfu/25g was reliably detected. To accomplish the second task of the project, the Applied Molecular Genomics Group of the VIB Department of Molecular Genetics (UA-VIB) designed a proprietary 33-amplicon multiplex PCR (mPCR) assay combined with capillary electrophoresis. This mPCR assay contains the detection of 5 STEC serotypes (O26, O103, O111, O145, O157), the main virulence genes VT1 with variants (VT1ab, VT1c and VT1d), VT2 with six variants (VT2b,c,d,e,f,g) and consensus, eae with five variants (eaeα1, eaeβ1, eaeγ1; eaeγ2; eaeε and eaeζ), ehx, tir, katP, saa, espP and FliC H2, H7, H8, H11 and H28. The assay was optimized and validated on a set of test strains representative for the priority amplicons. Next, this molecular technology was validated on a collection of 334 human clinical and animal strains from the Belgian STEC Reference Center (UZ). This collection of human and animal strains was also characterized by performing the PulseNet Europe protocol for pulsed field gel electrophoresis (PFGE). This technique creates a fingerprint of a strain by means of rare cutter restriction enzyme cutting of DNA and gel electrophoresis. Analysis of the band patterns lead to clustering of strains according to similarity or relatedness. Then results of 33-mPCR and PFGE genotyping were combined to show eventual correlations between PFGE genotypes and virulence profiles. Also background information about the strains (date of isolation, human or animal source, clinical manifestation, outbreak information) was included to the analysis. Combining mPCR and PFGE genotyping results, correlations were shown. In the first place STEC strains were clustered according to their serotype. Secondly a correlation occurred between virulence profile and PFGE clustering, concerning VT genes and other genes. Particularly for STEC O157, strains had very diverse VT-profiles, and strains with the same VT-profile clustered together. Concerning the clinical manifestation, ‘asymptomatic’ cases occurred more frequently for non-O157 than for O157 STEC, but besides this no correlation was shown between the PFGE clustering and the clinical manifestation or between the VT-profile and the clinical manifestation. Finally several case studies could be appointed based on the PFGE dendrograms. In general the cases contained clones that persisted during several years, had similar virulence profiles and infected humans as well as animals. As a part of the second task, the UA-VIB also designed a derived 9-amplicon multiplex PCR (9-mPCR) for fast sample screening. Using this 9-mPCR, a combination of serotypes (O26, O103, O111, O145, O157) and virulence genes (VT1, VT2, eae and ehx) is detected in one run and can be visualized using conventional gel electrophoresis. Once the 9-mPCR was developed and tested on pure strains, an evaluation on samples was performed. Hereto ILVO (Institute for Agricultural and Fisheries Research) tested several methods to extract DNA from artificially inoculated samples. Methods were compared based on the ability to remove PCR inhibiting molecules and on the ability to isolate and purify DNA from STEC cells. Out of four methods only two methods, in which no removal of sample debris was done, were suitable for sample preparation. The method using bead beating cell lysis described by Yu and Morrison (2004), was at least 10 times more sensitive than the method using the Qiagen Stool Mini Kit according to the manufacturer’s instructions, and was therefore recommended. However, the method using bead beating cell lysis is much more time consuming than the Qiagen method and the use of a ribolyser is necessary. As ILVO used the method employing the ribolyser in all following experiments, this method was used on artificially inoculated samples to determine its detection limit. All virulence marker genes and the serotype gene of strain MB3901 (serotype O157) could be detected in enriched minced beef and cheese from raw milk artificially inoculated with 2 cfu/25g sample. For cattle fecal samples the screening test was 10 times less sensitive; 21 cfu/25g feces could be detected. Finally the influence of the volume of lysate used in the mPCR reaction mix was examined. An mPCR reaction containing 1 and 2µl of lysate DNA was performed, but no difference in detection was seen. Testing of different clinical isolates of non-O157 STEC on the newly designed selective agars, showed that growth characteristics were generally as expected. However, more standardization of the preparation of the medium is needed to obtain more reproducible results. Some O103 isolates did not grow on the media prepared at UZ and the color of the colonies of O111 was often difficult to distinguish from O26. Using artificially contaminated stool samples, the sensitivity of the STEC isolation protocol developed in a previous Belspo SPSD II project was similar to the protocol used routinely at UZ (103 and 104 cfu/5g). The sensitivity was about 10 times higher when using IMS. The method performed well on frozen STEC positive samples, but this could only be tested on 14 samples, of which 11 with O157, 2 with O111 and one O26. In-house validation of the STEC isolation protocol was performed to evaluate if the protocol is applicable for different types of food matrices. All samples used for this validation were artificially contaminated. Ten samples of minced beef, raw milk cheese and sprouted seeds were artificially inoculated with varying numbers (10-2000 cfu/25g) of non-stressed and stressed strains belonging to the serotypes O157 (s-) and (s+), O26, O103, O111 and O145. Cultured STEC strains were cold and freeze stressed by storing them for at least 5 days at respectively 2 and -18°C. Inoculated samples were pre-enriched in a weak selective medium for 6 hours followed by enrichment in a stronger selective medium for 18 hours. Direct plating on a selective medium was performed after each enrichment step. In a third pathway, an IMS (Dynabeads or Captivate beads) step was performed after 24h enrichment and prior to plating. Suspected colonies on the selective medium were purified and tentatively confirmed on a purification medium followed by a confirmation by a serotype PCR. Parallel to the classical isolation method, the 9-mPCR screening test was performed on the enrichment medium (after 24 hours enrichment). Results indicate that the isolation protocol as well as mPCR screening provide good detection of non-stressed and cold-stressed O26, O103, O157 (s+) and O145 in raw milk cheese and minced beef. Detection of the other non-stressed and cold-stressed serotypes (O111 and O157 (s+)) in raw milk cheese and minced beef and of all serotypes under freeze stressed conditions in minced beef was low or almost zero. Probably due to the high level of background flora, detection of any serotype in sprouted seeds was almost impossible even though inoculation numbers were as high as 2000 cfu/25g. Finally the optimized STEC detection and isolation methods were validated by an interlaboratory study performed by national and international laboratories (twelve laboratories in total). First, a pre-trial experiment was organized to give the collaborative laboratories the possibility to become familiar with the isolation method. Secondly, the actual interlaboratory study was performed. Products necessary to prepare all culture media (in-house-prepared: IHP) and ready-to-use selective agar culture media (ready-to-use: RTU) were sent to the participating laboratories, as well as a questionnaire and a document to report the results. For each participating laboratory, 20 samples of 25g of minced beef were prepared: one sample for the temperature measurement upon arrival, one for the enumeration of the total count, Enterobacteriaceae and E. coli, two blank samples and sixteen samples inoculated with single strains belonging to 4 serotypes at 2 levels of contamination in duplicate (30 cfu/g and 300 cfu/g). All strains were cold stressed. Samples were prepared the day of the shipment and had to be analyzed on a prefixed day. The University of Liège evaluated all results based on the recommendations of ISO 16140. Results showed no difference between RTU and IHP media. The arabinose test seemed difficult to be read, so the dulcitol test is now preferred for the confirmation of serotypes O103 and O111. Some mistakes were made during sample inoculation, like a wrong inoculation of four samples and no inoculation of one sample. If we do not take into account these mistakes, all four serotypes were detected with high sensitivity. In general it can be concluded that the laboratory performance is highly satisfactory. [less ▲]

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See detailModal testing of nonlinear vibrating structures based on a nonlinear extension of force appropriation
Peeters, Maxime ULg; Kerschen, Gaëtan ULg; Golinval, Jean-Claude ULg

in ASME International Design Engineering Technical Conferences, San Diego, 2009 (2009)

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See detailA comparison of different types of commercial FBP and OSEM SPECT reconstruction software
Seret, Alain ULg; Forthomme, Julien

in Journal of Nuclear Medicine Technology (2009), 37(3), 179-187

This study aimed at comparing the performance of filtered backprojection (FBP) and ordered subset expectation maximisation (OSEM) reconstruction algorithms available in several types of commercial SPECT ... [more ▼]

This study aimed at comparing the performance of filtered backprojection (FBP) and ordered subset expectation maximisation (OSEM) reconstruction algorithms available in several types of commercial SPECT software. Methods: Numerical simulations of SPECT acquisitions of two phantoms were used: the NEMA line used for the assessment of SPECT resolution and a phantom with uniform, hot and cold rod compartments. The investigated types of software were: General Electric Xeleris and Vision, Philips Jetstream, Segami Mirage, Siemens eSoft and Icon, and Sopha Medical Vision XT. For FBP, no filtering and filtering of the projections with either Butterworth (order 3 or 6) or Hanning filters at various cut-off frequencies were considered. For OSEM, the number of subsets was 1, 4, 8, or 16 and the number of iterations was chosen to obtain a product number of iterations times the number of subsets equal to 16, 32, 48 or 64. The line phantom enabled us to obtain the reconstructed central, radial and tangential full-widths at half-maximum. The uniform compartment of the second phantom delivered the mean reconstructed pixel values and the standard deviations from which the coefficients of variation were calculated. Hot and cold contrasts were obtained from its rod compartments. Results: For FBP, full-widths at half-maximum, mean pixel values, coefficients of variation and contrasts were almost software independent. The only exceptions were: smaller (0.5 mm) full-widths at half-maximum for Vision, larger mean pixel values for Vision and XT, and better contrasts for Vision and XT for some filtering conditions. For OSEM, full-widths at half-maximum differed between the different types of software from 0.1 to 2.5 mm but these were almost independent of the number of subsets or iterations. There was a high dependence of the mean pixel value on the type of software used and a moderate dependence of the coefficient of variation. The contrasts were almost software independent. Mean pixel value varied greatly with the number of iterations for Mirage and Vision, and the coefficient of variation increased with the number of iterations for all types of software. The mean pixel value, the coefficient of variation and the contrasts were almost constant for a fixed product number of iterations times the number of subsets, whatever the number of subsets or iterations. Conclusion: Most of the types of software were equivalent for FBP or OSEM reconstruction. However, a few differences were observed for some types of software and should be considered when using them. [less ▲]

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See detailLes contrats commerciaux
Bublot, Jean ULg

in Guide des contrats et lettres types (2009)

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