Les principales techniques de neuroimagerie fonctionnelle et leur utilité pour l’étude du substrat neuronal du développement cognitif typique et atypique
Majerus, Steve ;
in Poncelet, Martine; Majerus, Steve; Van der Linden, Martial (Eds.) Traité de Neuropsychologie de l'Enfant (2009)Detailed reference viewed: 30 (5 ULg)
Cross-reactive T cell responses in HIV CRF01_AE and B'-infected intravenous drug users: implications for superinfection and vaccines.
; Thielen, Caroline ; et al
in AIDS Research and Human Retroviruses (2009), 25(1), 73-81
Abstract We previously observed limited cross-reactive T cell responses in two HIV-1-superinfected injection drug users (IDUs) before superinfection [Ramos A, et al.: J Virol 2002;76(15):7444-7452]. To ... [more ▼]
Abstract We previously observed limited cross-reactive T cell responses in two HIV-1-superinfected injection drug users (IDUs) before superinfection [Ramos A, et al.: J Virol 2002;76(15):7444-7452]. To elucidate the role of such responses in superinfection we examined cross-reactive T cell responses in IDUs infected with a single HIV-1 subtype. In this study, IFN-gamma ELISPOT assays were performed using recombinant vaccinia constructs and peripheral blood mononuclear cells (PBMCs) from 43 IDUs singly infected with CRF01_AE or B' from the same cohort as the superinfected IDUs. PBMCs were from time points corresponding to pre- (early) or post- (late) superinfection in the superinfected IDUs. We observed that most singly infected IDUs had cross-reactivity in samples from early (84% of CRF01_AE and 78% of B'-infected IDUs) and late (96% of CRF_01AE and 77% of B'-infected IDUs) time points. Frequent homologous reactivity at early (67% of CRF-01AE and 100% of B') and late (84% of CRF01_AE-infected and 100% of B'-infected IDUs) time points was also observed. Cross-reactive responses were predominantly to Pol and were broader and higher in CRF01_AE than in B'-infected IDUs (medians of 825 vs. 90 and 585 vs. 60 spot-forming units/10(6) PBMCs at early and late time points, respectively). Our results show that cross-reactive responses were more prevalent with greater height and breadth in singly infected IDUs than previously observed in corresponding collection time points of superinfected IDU. Thus, low or absent cross-reactivity may have contributed to the previously observed superinfections. These data are relevant for understanding superinfection and improving vaccine design. [less ▲]Detailed reference viewed: 41 (3 ULg)
Influence of microstructure on the thermal conductivity of magnetoresistive La0.7Ca0.3MnO3/Mn3O4 manganite/insulating oxide polycrystalline bulk composites
; Vertruyen, Bénédicte ; et al
in Journal of Applied Physics (2009), 105(6), 063501
We report the temperature dependence of the thermal conductivity kappa(T) of bulk polycrystalline composite samples containing a magnetoresistive manganite (La0.7Ca0.3MnO3) and an electrically insulating ... [more ▼]
We report the temperature dependence of the thermal conductivity kappa(T) of bulk polycrystalline composite samples containing a magnetoresistive manganite (La0.7Ca0.3MnO3) and an electrically insulating phase (Mn3O4). The sample porosity is shown to be a significant parameter affecting the experimental data: after porosity correction the curves display the characteristics of an ideal composite. A fit of the kappa(T) curves at low temperature using the Debye model enables the mean free path of phonons scattered on "boundaries" to be determined. The values are on the order of the grain size but are influenced by the grain arrangement and the presence of twins. [less ▲]Detailed reference viewed: 87 (25 ULg)
False phylogenies on wood mice due to cryptic cytochrome-b pseudogene.
; Michaux, Johan ; et al
in Molecular Phylogenetics & Evolution (2009), 50(3), 633-41
The phylogeny and phylogeography of the Old World wood mice (subgenus Sylvaemus, genus Apodemus, Muridae) are well-documented. Nevertheless, the distributions of species, such as A. fulvipectus and A ... [more ▼]
The phylogeny and phylogeography of the Old World wood mice (subgenus Sylvaemus, genus Apodemus, Muridae) are well-documented. Nevertheless, the distributions of species, such as A. fulvipectus and A. ponticus remain dubious, as well as their phylogenetic relationships with A. sylvaticus. We analysed samples of Apodemus spp. across Europe using the mitochondrial cytochrome-b gene (cyt-b) and compared the DNA and amino-acid compositions of previously published sequences. The main result stemming from this study is the presence of a well-differentiated lineage of Sylvaemus including samples of various species (A. sylvaticus, A. fulvipectus, A. ponticus) from distant locations, which were revealed to be nuclear copies of the mitochondrial cyt-b. The presence of this cryptic pseudogene in published sequences is supported by different pathways. This has led to important errors in previous molecular trees and hence to partial misinterpretations in the phylogeny of Apodemus. [less ▲]Detailed reference viewed: 7 (0 ULg)
Hybridization of short complementary PNAs to G-quadruplex forming oligonucleotides: An electrospray mass spectrometry study.
; ; De Pauw, Edwin et al
in Biopolymers (2009), 91(4), 244-255
We investigated the interaction of the short PNA strand [acccca]-PNA with oligodeoxynucleotides containing one, two, or four tracts of TGGGGT units. Electrospray ionization mass spectrometry allowed ... [more ▼]
We investigated the interaction of the short PNA strand [acccca]-PNA with oligodeoxynucleotides containing one, two, or four tracts of TGGGGT units. Electrospray ionization mass spectrometry allowed exploring the wide variety of complex stoichiometries that were found to coexist in solution. In water, the PNA strand forms short heteroduplexes with the complementary DNA sequences, but higher-order structures are also found, with PNA(2n)*DNA(n) triplex units, culminating in precipitation at very low ionic strength. In the presence of ammonium acetate, there is a competition between PNA*DNA heteroduplex formation and DNA G-quadruplex formation. Heteroduplex formation is favored when the PNA + DNA mixture in ammonium acetate is heated and cooled at room temperature, but not if the PNA is added at room temperature to the pre-formed G-quadruplex. We also found that the short [acccca]-PNA strand binds to G-quadruplexes. (c) 2008 Wiley Periodicals, Inc. Biopolymers, 2008. [less ▲]Detailed reference viewed: 98 (22 ULg)
Les troubles spécifiques du développement du langage.
Majerus, Steve ;
in Poncelet, Martine; Majerus, Steve; Van der Linden, Martial (Eds.) Traité de Neuropsychologie de l'Enfant (2009)Detailed reference viewed: 69 (12 ULg)
Molecular classification of T-cell lymphomas.
De Leval, Laurence ; Bisig, Bettina ; Thielen, Caroline et al
in Critical Reviews in Oncology/Hematology (2009)
T-cell neoplasms encompass a heterogeneous group of relatively rare disease entities. This review, focused on lymphoblastic tumors (T-ALL/LBL) and nodal-based peripheral T-cell lymphomas (PTCL ... [more ▼]
T-cell neoplasms encompass a heterogeneous group of relatively rare disease entities. This review, focused on lymphoblastic tumors (T-ALL/LBL) and nodal-based peripheral T-cell lymphomas (PTCL), summarizes recent advances in the molecular characterization of these diseases. In T-ALL/LBL, molecular subgroups delineated by gene expression profiling correlate with leukemic arrest at specific stages of normal thymocyte development and different oncogenic pathways, and seem to be of interest for prognosis prediction. Angioimmunoblastic T-cell lymphoma (AITL), one of the most common PTCL entities, comprises neoplastic cells with a molecular signature similar to normal follicular helper T cells, and this cellular derivation might account for several of the peculiar aspects of this disease. Except in ALK-positive anaplastic large cell lymphoma, defined by ALK gene fusions, chromosomal translocations are otherwise rare in PTCLs, but some recurrent rearrangements might be associated with distinct lymphoma subtypes. In PTCL, not otherwise specified (PTCL, NOS), novel molecular biomarkers of potential therapeutic interest have been recently identified. [less ▲]Detailed reference viewed: 52 (7 ULg)
Nuclear (scintigraphic) methods and FDG-PET in rheumatoid osteoarthritis
Ribbens, Clio ; Hustinx, Roland
in Bruno, Michael A.; Mosher, Timothy J.; Gold, Gary E. (Eds.) Arthritis in Color. Advance Imaging of Arthritis. (2009)Detailed reference viewed: 26 (7 ULg)
Invasions biologiques: un peu de vocabulaire
; ; et al
in Silva Belgica (2009)Detailed reference viewed: 84 (8 ULg)
Pertussis in adulthood: report of two cases and review of the literature.
Poirrier, Anne-Lise ; Gillard, Noelle ; Lefèbvre, Philippe et al
in Laryngoscope (2009), 119(9), 1720-2
Whooping cough is resurgent in the developed world. Systematic vaccination has changed its epidemiology, with the majority of cases now primarily affecting adolescents and adults. A 46-year-old male ... [more ▼]
Whooping cough is resurgent in the developed world. Systematic vaccination has changed its epidemiology, with the majority of cases now primarily affecting adolescents and adults. A 46-year-old male physiotherapist presented with a 1-week history of bothersome cough and respiratory difficulties, and a 51-year-old man was admitted to the emergency department with a 4-week history of increasing cough and dyspnea. Polymerase chain reaction of nasopharyngeal swab were positive for Bordetella pertussis. These cases illustrate pertussis in adulthood. We review the clinical features, the prevalence, the diagnostic tools, and the management of the patients and their relatives to increase awareness of this highly contagious disease. [less ▲]Detailed reference viewed: 27 (3 ULg)
Les clauses exonératoires ou limitatives de responsabilité insérées dans les conditions générales: leurre ou évidence?
Kohl, Benoît ; Grisard, Delphine
in Les conditions générales - Questions particulières (2009)Detailed reference viewed: 204 (15 ULg)
Conditional knockout of nucleolin in DT40 cells reveals the functional redundancy of its RNA-binding domains.
; Thiry, Marc ;
in Biology of the Cell (2009), 101(3), 153-67
BACKGROUND INFORMATION: Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a ... [more ▼]
BACKGROUND INFORMATION: Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nucleolin influences cell proliferation remained elusive so far. RESULTS: We have generated conditional nucleolin-knockout cells using the chicken B lymphocyte cell line DT40. Our results indicate that nucleolin is absolutely required for the proliferation and for the survival of these cells. Depletion of nucleolin drastically inhibits rDNA (ribosomal DNA) transcription while only slightly affecting pre-rRNA processing. This inhibition is accompanied by modifications of the shape and the structure of the nucleolus. The analysis of mutants of nucleolin, which lack two or three RNA-binding domains, shows that these domains harbour redundant functions and that nucleolin's roles in transcription, rRNA maturation and nucleolar shape can be partially uncoupled. CONCLUSIONS: The function of nucleolin in ribosomal synthesis could account for its effect on cell division and survival, but this vital role does not seem to be linked to sequence-specific RNA binding. [less ▲]Detailed reference viewed: 29 (6 ULg)
Tomography of the cell nucleus using confocal microscopy and medium voltage electron microscopy.
; ; Thiry, Marc et al
in Critical Reviews in Oncology/Hematology (2009), 69(2), 127-43
Changes in nuclear structures are widely used by pathologists as diagnostic and prognostic indicators in cancer cells. Recent studies have demonstrated that the cell nucleus is probably the most complex ... [more ▼]
Changes in nuclear structures are widely used by pathologists as diagnostic and prognostic indicators in cancer cells. Recent studies have demonstrated that the cell nucleus is probably the most complex organelle in the cell. It contains the genome and is the site of all related activities such as DNA repair, DNA duplication, RNA synthesis, RNA processing and RNA transport. These activities take place within dynamic three-dimensional compartments. The detailed study of these compartments requires an approach termed "cell tomography" based on 3D imaging using confocal microscopy and electron tomography. In this paper, we will first summarize the most recent findings concerning the organization of the cell nucleus. We will then describe markers used to identify molecules specific for various nuclear compartments and their use in tomography of the cell nucleus by confocal microscopy and electron tomography. [less ▲]Detailed reference viewed: 32 (1 ULg)
Clinical, virological, and immunological parameters associated with superinfection of latently with FeHV-1 infected cats
; ; et al
in Veterinary Microbiology (2009)Detailed reference viewed: 30 (7 ULg)
Concentrations of pregnancy-associated glycoproteins in Water buffaloes females (Bubalus bubalis) during pregnancy and postpartum periods
; Melo de Sousa, Noelita ; et al
in O’Leary, M.; Arnett, J. (Eds.) Pregnancy Protein Research (2009)
The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708 ... [more ▼]
The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708). Samples (10 mL) were collected by jugular venipuncture from Week 0 until Week 28 of pregnancy (9 females), and from parturition until Week 10 postpartum (13 females). During pregnancy, PAG molecules were detectable at Week 6 using the three aforementioned RIA systems (3.9 ± 1.3 ng/mL, 9.7 ± 1.3 ng/mL, and 9.9 ± 0.7 ng/mL for RIA-497, RIA-706, and RIA-708, respectively). These concentrations increased gradually until Week 28, reaching values of 39.6 ± 4.0 ng/mL (RIA-497), 50.5 ± 11.9 ng/mL (RIA-706), and 68.2 ± 20.8 ng/mL (RIA-708). PAG concentrations determined by RIA-497, RIA-706, and RIA-708 were strongly correlated throughout the entire gestation period, RIA-708 giving the higher concentrations. At parturition, the mean concentrations ranged from 34.9 ± 4.0 (RIA-497) to 84.7 ± 10.6 ng/mL (RIA-708). Thereafter, the concentrations decreased steadily, reaching very low levels (< 1.0 ng/mL) at Week 8 postpartum. In conclusion, PAG concentrations measured by the above three RIA systems showed a profile similar to those previously described in bovine species, with higher concentrations being detected by RIA-706 and RIA-708. However, the patterns of PAG concentrations, studied using the three aforementioned PAG-RIA systems, differed around parturition, with very low levels being observed in the female buffaloes [less ▲]Detailed reference viewed: 71 (4 ULg)
Modelling the uptake and diffusion of innovative technology: a critical and strategic feature of budget impact analysis and risk-sharing agreements.
Ethgen, Olivier ; Hiligsmann, Mickaël ; Reginster, Jean-Yves
in Value in Health (2009)Detailed reference viewed: 41 (9 ULg)
Effect of a single i.v. infusion of zoledronic acid on bone turnover markers versus oral risedronate in patients with glucocorticoid-induced osteoporosis.
; ; Reginster, Jean-Yves et al
in Bone (2009)Detailed reference viewed: 13 (1 ULg)
La pharmacie et ses usagers : perspectives éthiques
in Tous dopés? Ethique de la médecine d'amélioration (2009)Detailed reference viewed: 17 (1 ULg)
The fluid trampoline: droplets bouncing on a soap film
Gilet, Tristan ;
in Journal of Fluid Mechanics (2009), 625
We present the results of a combined experimental and theoretical investigation of droplets falling onto a horizontal soap film. Both static and vertically vibrated soap films are considered. In the ... [more ▼]
We present the results of a combined experimental and theoretical investigation of droplets falling onto a horizontal soap film. Both static and vertically vibrated soap films are considered. In the static case, a variety of behaviours were observed, including bouncing, crossing and partial coalescence. A quasi-static description of the soap film shape yields a force–displacement relation that provides excellent agreement with experiment, and allows us to model the film as a nonlinear spring. This approach yields an accurate criterion for the transition between droplet bouncing and crossing. Moreover, it allows us to rationalize the observed constancy of the contact time and scaling for the coefficient of restitution in the bouncing states. On the vibrating film, a variety of bouncing behaviours were observed, including simple and complex periodic states, multi-periodicity and chaos. A simple theoretical model is developed that captures the essential physics of the bouncing process, reproducing all observed bouncing states. The model enables us to rationalize the observed coexistence of multiple periodic bouncing states by considering the dependence of the energy transferred to the droplet on the phase of impact. Quantitative agreement between model and experiment is deduced for simple periodic modes, and qualitative agreement for more complex periodic and chaotic bouncing states. Analytical solutions are deduced in the limit of weak forcing and dissipation, yielding insight into the contact time and periodicity of the bouncing states. [less ▲]Detailed reference viewed: 26 (1 ULg)
Causes et conséquences de la dissuasion nucléaire israélienne
in Cahiers de Sciences Politiques de l'ULg (2009)Detailed reference viewed: 52 (10 ULg)