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See detailEpithelial-mesenchymal transition process in human embryonic stem cells cultured in feeder-free conditions.
Ullmann, U.; In'T Veld, P.; Gilles, Christine ULg et al

in Molecular Human Reproduction (2007), 13(1), 21-32

Feeder-free human embryonic stem cell (hESC) culture is associated with the presence of mesenchymal-like cells appearing at the periphery of the colonies. The aim of this study was to identify this early ... [more ▼]

Feeder-free human embryonic stem cell (hESC) culture is associated with the presence of mesenchymal-like cells appearing at the periphery of the colonies. The aim of this study was to identify this early differentiation process. Long-term feeder-free hESC cultures using matrigel and conditioned medium from mouse and from human origin revealed that the appearance of mesenchymal-like cells was similar regardless of the conditioned medium used. Standard characterization confirmed the preservation of hESC properties, but the feeder-free cultures could not be maintained longer than 37 passages. The early differentiation process was characterized in the short term after switching hESCs cultured on feeders to feeder-free conditions. Transmission electron microscopy showed an epithelium-like structure inside the hESC colonies, whereas the peripheral cells revealed the acquisition of a rather mesenchymal-like phenotype. Immunochemistry analysis showed that cells at the periphery of the colonies had a negative E-cadherin expression and a positive Vimentin expression, suggesting an epithelial–mesenchymal transition (EMT). Nuclear staining of ß-catenin, positive N-cadherin and negative Connexin 43 expression were also found in the mesenchymal-like cell population. After RT–PCR analysis, Slug and Snail, both EMT-related transcription factors, were detected as up-regulated in the mesenchymal-like cell population. Taken together, our data suggest that culturing hESCs in feeder-free conditions enhances an early differentiation process identified as an EMT. [less ▲]

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See detailAmputation in diabetic patients.
Van Damme, Hendrik ULg; Limet, Raymond ULg

in Clinics in Podiatric Medicine & Surgery (2007), 24(3), 569-82

In diabetes-related amputations, the risk of nonhealing or infection of a wound and the need for revision are increased. Medical treatment before amputation should optimize general and local conditions ... [more ▼]

In diabetes-related amputations, the risk of nonhealing or infection of a wound and the need for revision are increased. Medical treatment before amputation should optimize general and local conditions including the regression of edema, the control of infection, and the optimization of glucoregulation. A major argument for foot-sparing surgery is the poor functional recovery after major limb amputation. Diabetic patients are frail, with an increased postoperative morbidity and mortality after major amputation. Factors detrimental to functional outcome are advanced age, end-stage renal disease, dementia, and above-knee amputation. A multidisciplinary approach is required to optimize the results of diabetes-related amputations. The authors discuss medical and technical aspects that may reduce the failure of minor or major diabetes-related amputations. [less ▲]

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See detailCorrosion patina or intentional patina: Contribution of non-destructive analyses to the surface study of copper based archaeological objects
Mathis, François ULg; Descamps, Sophie; Delange, Elisabeth et al

in Dillmann, Philippe; Piccardo, Paolo; Matthiesen, H. (Eds.) et al Corrosion of metallic heritage artefacts: Investigation, conservation and prediction of long term behaviour (EFC 48) (2007)

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See detailLes industries aurignaciennes et "aurignacoïdes" en Moldavie après 30.000 BP
Noiret, Pierre ULg

in Annales de l'Université "Valahia" Târgoviste, Section d'Archéologie et d'Histoire (2007), IX

The lithic inventories from several sites excavated in Romania and in the Moldovan Republic suggest the existence of a Late Aurignacien around 27,500 BP, then of an Epi-Aurignacian, more recent, since 21 ... [more ▼]

The lithic inventories from several sites excavated in Romania and in the Moldovan Republic suggest the existence of a Late Aurignacien around 27,500 BP, then of an Epi-Aurignacian, more recent, since 21,000 BP. If the first seems to be a late expression of the Typical Aurignacian, the second corresponds only to a resurgence of some Aurignacian-like techniques, without any direct filiation. This phenomenon is, anyway, observable throughout Central and Eastern Europe and its connections with the Epigravettian are still barely known. [less ▲]

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See detailThe Asian Crisis Exchange Risk Exposure of U.S. Multinationals
Muller, Aline ULg; Verschoor, Willem

in Managerial Finance (2007), 33(9), 710-740

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See detailThe effect of high CO2 during the second half of incubation on the acid-base balance regulation in the chicken embryo
Everaert, Nadia ULg; Linser, P.; Decuypere, E. et al

in Avian and Poultry Biology Reviews (2007)

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See detailL'écrivain et ses doubles. Les Deux Consciences de Camille Lemonnier
Denis, Benoît ULg

in Textyles : Revue des Lettres Belges de Langue Française (2007), (31), 60-73

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See detailPerturbation technique for the finite element modelling of differential probes in non-destructive eddy-current testing
V Sabariego, Ruth ULg; Dular, Patrick ULg

in IET Science, Measurement & Technology (2007), 1(1), 25-29

The paper deals with a new finite element scheme for non-destructive eddy-current testing (ECT) problems involving multiply connected test pieces and differential probes. It concerns a perturbation ... [more ▼]

The paper deals with a new finite element scheme for non-destructive eddy-current testing (ECT) problems involving multiply connected test pieces and differential probes. It concerns a perturbation technique applied to the magnetodynamic h - phi formulation. The unperturbed field (in the absence of the flaw) is conventionally computed in the complete domain. The source of the perturbation problem is then determined by the projection of the unperturbed field in a relatively small region around the defect, the optimum size of which depends on the working frequency. The discretisation of this reduced domain is well adapted to the size of the defect and chosen independently of the dimensions of the excitation probe and the specimen under study. At a discrete level, the voltage change is efficiently computed by integration only over the defect and a layer of elements in the reduced domain that touches the defect's boundary. The accuracy of the proposed perturbation model is illustrated by comparison of the results obtained for different dimensions of the reduced domain with those achieved in the conventional way. The considered test case involves a differential probe scanning the outer surface of a metal tube for the detection of through-wall cracks. [less ▲]

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See detailSuivi 2006 du phytoplancton des eaux côtières corses : évaluation de la stratégie d’échantillonnage proposée dans le cadre des contrôles de surveillance de la DCE et première caractérisation des masses d’eau
Goffart, Anne ULg

Report (2007)

Les résultats obtenus dans le cadre du suivi DCE 2006 du phytoplancton des eaux côtières corses ont permis: − de tester et de valider une stratégie d’échantillonnage originale adaptée aux réseaux de ... [more ▼]

Les résultats obtenus dans le cadre du suivi DCE 2006 du phytoplancton des eaux côtières corses ont permis: − de tester et de valider une stratégie d’échantillonnage originale adaptée aux réseaux de surveillance et aux spécificités régionales; − de caractériser globalement les communautés phytoplanctoniques de 6 masses d’eau côtières ne présentant pas de risque NABE à l’horizon 2015 (Calvi, Cargèse, Pianottoli, Est Cap Corse, Sud Bastia et Sant’Amanza); - de démontrer le très bon état des masses d’eaux échantillonnées pour l’indicateur Phytoplancton; - d’émettre des recommandations visant à optimiser l’effort d’échantillonnage et la validité des résultats. La stratégie d’échantillonnage testée et validée propose de réaliser deux suivis « haute fréquence » au niveau des sites de référence de Calvi et de la station « Sud Bastia ». L’échantillonnage doit être réalisé en subsurface toute l’année, une fois par semaine de janvier à juin et une fois par quinzaine de juillet à décembre. Les autres masses d’eau sont échantillonnées une fois par mois, de janvier à décembre. Les résultats 2006 mettent en évidence les caractéristiques communes des communautés phytoplanctoniques des masses d’eau de Calvi, Cargèse et Pianottoli et des masses d’eau Est Cap Corse, Sud Bastia et Sant’Amanza. Ceci suggère que les suivis réalisés dans le cadre des contrôles de surveillance pourraient se limiter aux observations « haute fréquence » réalisées à Calvi et à la station « Sud Bastia ». [less ▲]

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See detailA propos de l'épistémologie personnelle : un état des recherches anglo-saxonnes
Crahay, Marcel ULg; Fagnant, Annick ULg

in Revue Française de Pédagogie (2007), 161

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See detailDry-season status, trend and distribution of Konkombouri elephants and implications for their management, Burkina Faso
Bouché, Philippe ULg

in Pachyderm (2007), 42

Konkombouri Hunting Zone is part of the transfrontier W–Arly–Pendjari ecosystem. Monthly dry-season records from wildlife monitoring show that the free-ranging elephant population increased from month to ... [more ▼]

Konkombouri Hunting Zone is part of the transfrontier W–Arly–Pendjari ecosystem. Monthly dry-season records from wildlife monitoring show that the free-ranging elephant population increased from month to month with the progression of the dry season and the reduction of water points that still contained water. However, with the arrival of the first important rains, elephant density continued to increase because water and new shoots were available everywhere. In 2005 and 2006 elephant density in the Konkombouri Hunting Zone reached the highest mean dry-season elephant density recorded in West Africa in recent decades. This situation increases the impact on habitat close to water points and heightens human–elephant conflicts. [less ▲]

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See detailL'assiette du Belge en chiffres
Duquesne, Brigitte ULg

Poster (2007)

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See detailAsian Foreign Exchange Risk Exposure
Muller, Aline ULg; Verschoor, Willem

in Journal of the Japanese & International Economies (2007), 21(1), 16-37

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See detailLe droit international humanitaire à l’Université de Liège : une expérience qui porte ses fruits…
Collienne, Fleur ULg; Jullien, Gaëlle ULg; Guerra, Vincent ULg

in Revue de la Faculté de Droit de l'Université de Liège (2007)

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See detailLe secteur de la viande bovine dans l’Union européenne : situation et perspectives dans le contexte de l’environnement
Burny, Philippe ULg

in Sudia Universitatis, Babes-Bolyai, Ambientum I/1-2, 2007 (2007)

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See detailNumerical simulation of semi-solid thixoforming
Koeune, Roxane ULg; Ponthot, Jean-Philippe ULg

in Int. Conf. on Computational Plasticity, COMPLAS IX (2007)

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See detailComment je traite...un uterus didelphe associe a un hemi-vagin borgne
Loumaye, F.; Cheruy, Charlotte ULg; Cristinelli, S. et al

in Revue Médicale de Liège (2007), 62(7-8, Jul-Aug), 480-3

Didelphys uterus with imperforated obstructed hemivagina is a rare condition. Most often, it is diagnosed a few months after the menarche. Hematocolpos, hematometria and sometimes hematosalpinx are ... [more ▼]

Didelphys uterus with imperforated obstructed hemivagina is a rare condition. Most often, it is diagnosed a few months after the menarche. Hematocolpos, hematometria and sometimes hematosalpinx are responsible for pelvic pain and dysmenorrhea. Symptoms can be delayed when a fistulisation from the hematocolpos to permeable contralateral vagina is present. Sometimes, diagnosis is not made before adulthood during an infection of the hematocolpos. Useful additional exams include pelvic ultrasound and, in some cases, MRI, which is the best exam to precisely describe the type of malformation. An ipsilateral agenesia of the kidney is always associated. Hysterography can usually demonstrate the fistulous courses. Treatment consists in a large resection of the vaginal septum in order to allow a permanent drainage of the hemiuterus. A laparoscopy should be performed to check for the presence of associated tubal or pelvic damage. [less ▲]

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