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See detailEstrus synchronization, artificial insemination, and pregnancy diagnosis in water buffaloes (Bubalus bubalis)
Hanh, NV; Huu, QX; Uoc, NT et al

in Reproduction, Fertility and Development (2007), 19(1), 198

One of the big obstacles in management of reproductive activity in water buffaloes is their silent estrus. This characteristic prevents the determination of time for artificial insemination (AI ... [more ▼]

One of the big obstacles in management of reproductive activity in water buffaloes is their silent estrus. This characteristic prevents the determination of time for artificial insemination (AI), resulting in lengthening of the calving interval (Nguyen et al. 1990 Proc. FAO Workshop, Bulgaria, 210–216). Pregnancy-associated glycoprotein (PAG) measurement in maternal blood is an effective method of early pregnancy diagnosis in ruminant species (Zoli et al. 1992 Biol. Reprod. 46, 83–92). The present study describes a preliminary attempt to improve buffalo reproductive performance by association of three different approaches: synchronization of estrus by hormonal treatment, followed by two AI, and finally pregnancy diagnosis based on PAG measurements. A total of 33 buffalo females (4 to 15 years old), with no apparent estrus during a period of 9 to 36 months after last calving, were used. Each female received 2 injections of 5 mL of PGF2α (Intervet, France) 10 days apart (Day -12 and Day -2), one injection of 500 IU of PMSG (SABC, Vietnam) at Day -5, and 1000 IU of hCG (Intervet, France) on the day of AI (Day 0). A second AI was performed 8 h after hCG injection. Blood samples (9 mL) were collected from each female at Days 20, 25, 40, and 45 after AI. Blood samples were withdrawn by jugular venipuncture (heparinized Vacutainer tubes) and kept in a cool box until centrifugation (500g, 10 min). Plasma samples were stored at -20°C until assayed for PAG. Pregnancy diagnosis was considered negative, doubtful, or positive according to the PAG concentrations (<0.6 ng mL-1, 0.6 to 0.8 ng mL-1, and >0.8 ng mL-1, respectively). Clinical observation of females after insemination showed that the total rate of animals that exhibited signs of estrus until Day 45 was 9.1% (3/33). These 3 females were not considered for pregnancy diagnosis. Table 1 shows the results of pregnancy diagnosis in 30 water buffalo females. These results show that fertilization is easily obtained after estrus synchronization in buffaloes and that measurement of PAG concentrations in maternal plasma can be used for pregnancy diagnosis from Day 40 after AI. [less ▲]

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See detailAsian Foreign Exchange Risk Exposure
Muller, Aline ULg; Verschoor, Willem

in Journal of the Japanese & International Economies (2007), 21(1), 16-37

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See detailEffect of base mutations in GC rich binding site on recognition by minor groove binding dications
Munde, M; Ismail, M; Peixoto, Paul ULg et al

Poster (2007)

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See detailThe Computation of Equating Errors in International Surveys in Education
Monseur, Christian ULg; Berezner, Alla

in Journal of Applied Measurement (2007), 8(3), 323-35

Since the IEA's Third International Mathematics and Science Study, one of the major objectives of international surveys in education has been to report trends in achievement. The names of the two current ... [more ▼]

Since the IEA's Third International Mathematics and Science Study, one of the major objectives of international surveys in education has been to report trends in achievement. The names of the two current IEA surveys reflect this growing interest: Trends in International Mathematics and Science Study (TIMSS) and Progress in International Reading Literacy Study (PIRLS). Similarly a central concern of the OECD's PISA is with trends in outcomes over time. To facilitate trend analyses these studies link their tests using common item equating in conjunction with item response modelling methods. IEA and PISA policies differ in terms of reporting the error associated with trends. In IEA surveys, the standard errors of the trend estimates do not include the uncertainty associated with the linking step while PISA does include a linking error component in the standard errors of trend estimates. In other words, PISA implicitly acknowledges that trend estimates partly depend on the selected common items, while the IEA's surveys do not recognise this source of error. Failing to recognise the linking error leads to an underestimation of the standard errors and thus increases the Type I error rate, thereby resulting in reporting of significant changes in achievement when in fact these are not significant. The growing interest of policy makers in trend indicators and the impact of the evaluation of educational reforms appear to be incompatible with such underestimation. However, the procedure implemented by PISA raises a few issues about the underlying assumptions for the computation of the equating error. After a brief introduction, this paper will describe the procedure PISA implemented to compute the linking error. The underlying assumptions of this procedure will then be discussed. Finally an alternative method based on replication techniques will be presented, based on a simulation study and then applied to the PISA 2000 data. [less ▲]

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See detailMechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human.
Gillet, Nicolas ULg; Florins, Arnaud-Francois ULg; Boxus, Mathieu ULg et al

in Retrovirology (2007), 4(1), 18

In 1871, the observation of yellowish nodules in the enlarged spleen of a cow was considered to be the first reported case of bovine leukemia. The etiological agent of this lymphoproliferative disease ... [more ▼]

In 1871, the observation of yellowish nodules in the enlarged spleen of a cow was considered to be the first reported case of bovine leukemia. The etiological agent of this lymphoproliferative disease, bovine leukemia virus (BLV), belongs to the deltaretrovirus genus which also includes the related human T-lymphotropic virus type 1 (HTLV-1). This review summarizes current knowledge of this viral system, which is important as a model for leukemogenesis. Recently, the BLV model has also cast light onto novel prospects for therapies of HTLV induced diseases, for which no satisfactory treatment exists so far. [less ▲]

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See detailThe Grolier Codex: A PIXE & RBS study of the possible Maya document
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Calderón, Tomás et al

Poster (2007)

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See detailNotions d'histoire de l'art et archéologie: Orient ancien
Van der Stede, Véronique ULg

Learning material (2007)

 Syllabus destiné aux étudiants de 2e et 3e année de BA. Il reprend la matière présentée dans le cadre du cours de « Notions d’histoire de l’art et d’archéologie : orient ancien », soit une sélection des ... [more ▼]

 Syllabus destiné aux étudiants de 2e et 3e année de BA. Il reprend la matière présentée dans le cadre du cours de « Notions d’histoire de l’art et d’archéologie : orient ancien », soit une sélection des artefacts et des vestiges les plus significatifs de la période comprise entre le Natoufien et le milieu du 1er millénaire av. n. è. Cette documentation archéologique est replacée dans son contexte historique et géographique. [less ▲]

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See detailWhole body vibration in the treatment of fibromyalgia: Influence on muscle performances
Maquet, Didier ULg; Helene, Loic; Demoulin, Christophe ULg et al

in Isokinetics & Exercise Science (2007), 15

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See detailLa confrontation de l’offre et de la demande mondiales de céréales fixe désormais le prix payé au producteur européen
Burny, Philippe ULg

in Faculté universitaire des Sciences agronomiques de Gembloux; Centre wallon de Recherches agronomiques (Eds.) 40 ans Livre blanc Céréales-Gembloux février 2007 (2007)

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See detailHow does the age of hoverfly females affect their reproduction?
Almohamad, Raki; Verheggen, François ULg; Francis, Frédéric ULg et al

in Communications in Agricultural and Applied Biological Sciences (2007), 72(3),

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See detailFabrication, characterization, and modeling of strained SOI MOSFETs with very large effective mobility
Driussi, F.; Esseni, D.; Selmi, L. et al

in IEEE (Ed.) 37th European Solid State Device Research Conference (ESSDERC) (2007)

Strained silicon on insulators (sSOI) wafers with a supercritical thickness of 58 nm were produced using thin strain relaxed SiGe buffer layers, wafer bonding, selective etch back and epitaxial overgrowth ... [more ▼]

Strained silicon on insulators (sSOI) wafers with a supercritical thickness of 58 nm were produced using thin strain relaxed SiGe buffer layers, wafer bonding, selective etch back and epitaxial overgrowth. Raman spectroscopy revealed an homogeneous strain of 0.63 plusmn 0.03 % in the strained Si layer. Long channel n-type SOI-MOSFETs showed very large electron mobilities up to 1200 cm2/Vs in the strained Si devices. These values are more than two times larger than those of reference SOI n-MOSFETs. Mobility simulations with state of the art scattering models are then used to interpret the experiments. [less ▲]

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See detailMigration et environnement : brève introduction à une relation méconnue et souvent négligée
Gemenne, François ULg

in Etopia : Revue d'Economie Politique (2007), 3

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See detailCrédit à la consommation et assurance: l'embrouillamini législatif, note sous J.P. Courtrai, 1e mars 2005
Maisetti, Perrine ULg

in Journal des Juges de Paix = Tijdschrift van de Vrederecters (2007)

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See detailDirecting the lipopeptide Mycosubtilin biosynthesis toward C17:0 branched isoform influences the expression of cspB and cspC in Bacillus subtilis
Guez, Jean-Sébastien; Drucbert, A.; Müller, C. et al

Poster (2007)

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See detailL’érythropoïèse tardive : une mort avortée ?
Courtois, Geneviève; Vandekerchove, julie; Dussiot, Michael et al

in Hématologie (2007), 13(6), 400-408

La survie et la prolifération des progéniteurs et précurseurs érythroïdes se trouvent sous le contrôle de l’érythropoïétine (Epo), qui est le principal régulateur de l’érythropoïèse. La différenciation ... [more ▼]

La survie et la prolifération des progéniteurs et précurseurs érythroïdes se trouvent sous le contrôle de l’érythropoïétine (Epo), qui est le principal régulateur de l’érythropoïèse. La différenciation érythroïde s’effectue sous la dépendance du facteur de transcription GATA-1 qui active l’expression des gènes de différenciation et de survie. La production des globules rouges est finement régulée par l’inhibition ou au contraire par l’induction de l’apoptose des progéniteurs et des précurseurs érythroïdes. La baisse du taux d’Epo circulante ou l’induction de la voie Fas aboutissent à l’activation de la caspase-3 qui entraîne la protéolyse de GATA-1, l’arrêt de maturation et l’apoptose des érythroblastes immatures. Nous avons montré qu’une activation transitoire de la caspase-3 par la voie mitochondriale est indispensable à la maturation érythroïde. Dans ce contexte, la protéine chaperonne hsp70 joue un rôle majeur en protégeant GATA-1 du clivage par la caspase-3. La différenciation terminale est caractérisée par une réduction progressive du volume cellulaire et du noyau associée à une condensation de la chromatine. Ces changements morphologiques présentent certaines similitudes avec ceux observés dans les cellules en cours d’apoptose. L’énucléation s’effectue ensuite au sein d’îlots érythroblastiques constitués d’un macrophage central étroitement associé par des molécules d’adhérence à des érythroblastes en cours de maturation. La membrane de l’érythroblaste perd progressivement son affinité pour le macrophage alors que le noyau reste fixé, ce qui permet l’énucléation et la phagocytose du noyau par le macrophage. Les réticulocytes ainsi formés vont compléter leur maturation en perdant leurs organelles et en remodelant leur membrane. Nos études suggèrent que le devenir des précurseurs érythroïdes (apoptose versus différenciation) est déterminé par le choix des cibles clivées par les caspases. Si la fonction du clivage de certaines protéines telles que la lamine B ou acinus est bien connue, l’identité et la fonction des autres cibles clivées par les caspases au cours de la maturation terminale restent à déterminer. [less ▲]

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See detailDefensis induce the recruitment of dendritic cells in cervical human papillomavirus-associated (pre)neoplastic lesions formed in vitro and transplanted in vivo
Hubert, Pascale ULg; Herman, Ludivine ULg; Maillard, Catherine ULg et al

in FASEB Journal (2007), 21(11), 2765-75

In addition to their direct antimicrobial activity, defensins might also influence adaptive immunity by attracting immature dendritic cells (DC). As these cells have been shown to be deficient in uterine ... [more ▼]

In addition to their direct antimicrobial activity, defensins might also influence adaptive immunity by attracting immature dendritic cells (DC). As these cells have been shown to be deficient in uterine cervix carcinogenesis, we evaluated the ability of -defensin (HNP-2, human neutrophil defensin 2) and ß-defensin (HßD2, human beta defensin 2) to stimulate their migration in human papillomavirus (HPV)-associated (pre)cancers. We first observed, using RT-PCR and immunohistology, that HßD2 is absent in HPV-transformed keratinocytes and that it is weakly expressed in cervical (pre)neoplastic lesions in comparison with normal keratinocytes. We next demonstrated that defensins exert a chemotactic activity for DC in a Boyden Chamber assay and stimulate their infiltration in an in vitro-formed (pre)neoplastic epithelium (organotypic culture of HPV-transformed keratinocytes). To evaluate the ability of defensins also to recruit DC in vivo, we developed a model of immunodeficient mice grafted with organotypic cultures of HPV+ keratinocytes, which form an epithelium similar to a high-grade neoplastic lesion, with tumoral invasion and neovascularization. Intravenously injected human DC were able to infiltrate grafts of HPV+ keratinocytes after administration of HNP-2 in the transplantation chamber. Taken together, these results suggest that defensins could reverse a frequent immune alteration observed in cancer development. [less ▲]

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