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See detailHistone deacetylase inhibitor trichostatin A sustains sodium pervanadate-induced NF-kappa B activation by delaying IkappaBalpha mRNA resynthesis : comparison with tumor necrosis factor alpha
Horion, Julie ULg; Gloire, Geoffrey ULg; El Mjiyad, Nadia et al

in Journal of Biological Chemistry (2007), 282(21), 15383

NF-kappaB is a crucial transcription factor tightly regulated by protein interactions and post-translational modifications, like phosphorylation and acetylation. A previous study has shown that ... [more ▼]

NF-kappaB is a crucial transcription factor tightly regulated by protein interactions and post-translational modifications, like phosphorylation and acetylation. A previous study has shown that trichostatin A (TSA), a histone deacetylase inhibitor, potentiates tumor necrosis factor (TNF) alpha-elicited NF-kappaB activation and delays IkappaBalpha cytoplasmic reappearance. Here, we demonstrated that TSA also prolongs NF-kappaB activation when induced by the insulino-mimetic pervanadate (PV), a tyrosine phosphatase inhibitor that initiates an atypical NF-kappaB signaling. This extension is similarly correlated with delayed IkappaBalpha cytoplasmic reappearance. However, whereas TSA causes a prolonged IKK activity when addedtoTNFalpha, it does notwhenaddedtoPV.Instead, quantitative reverse transcriptase-PCR revealed a decrease of ikappabalphamRNAlevel after TSA addition to PV stimulation. This synthesis deficit of the inhibitor could explain the sustained NF-kappaB residence in the nucleus. In vivo analysis by chromatin immunoprecipitation assays uncovered that, forPVinduction but not forTNFalpha, the presence of TSA provokes several impairments on the ikappabalphapromoter: (i) diminution of RNA Pol II recruitment; (ii) reduced acetylation and phosphorylation of histone H3-Lys14 and -Ser10, respectively; (iii) decreased presence of phosphorylated p65-Ser536; and (iv) reduction of IKKalphabinding. The recruitment of these proteins on the icam-1 promoter, another NF-kappaB-regulated gene, is not equally affected, suggesting a promoter specificity of PV with TSA stimulation. Taken together, these data suggest that TSA acts differently depending on the NF-kappaB pathway and the targeted promoter in question. This indicates that one overall histone deacetylase role is to inhibit NF-kappaB activation by molecular mechanisms specific of the stimulus and the promoter. [less ▲]

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See detailLe syndrome d'Ehlers-Danlos. Qu'y a-t-il sous la pointe émergée de l'iceberg?
Quatresooz, Pascale ULg; Blaise, Géraldine; Franchimont, Claudine ULg et al

in Dermatologie Actualité (2007), 105

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See detailPréface à "L'échec du livre électronique de Cytale" de D. Nauroy
Durand, Pascal ULg

in Nauroy, Dominique (Ed.) L'échec du livre électronique de Cytale au prisme des processus de traduction (2007)

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See detailLe Gravettien de Moldavie (30.000-23.000 BP)
Noiret, Pierre ULg

in Paléo. Revue d'Archéologie préhistorique (2007), 19

In Moldavia, three sequences serve as the basis for the chronological context for Gravettian (and Epigravettian) occupations. Two technological phases have been identified, showing independent development ... [more ▼]

In Moldavia, three sequences serve as the basis for the chronological context for Gravettian (and Epigravettian) occupations. Two technological phases have been identified, showing independent development, homogenous in both technological and typological attributes. The Gravettian is characterized by large retouched blades, followed by shouldered points (29,500-23,000 BP), succeeded by the Early Epigravettian (13,500-11,000 BP). [less ▲]

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See detailNavigation neurochirurgicale basée sur des modèles biomécaniques
Vigneron; Boman, Romain C.; Ponthot, Jean-Philippe et al

Conference (2007)

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See detailN-heterocyclic carbene ruthenium complexes: Synthesis and catalytic properties
Dragutan, Ileana; Dragutan, Valerian; Delaude, Lionel ULg et al

in Revue Roumaine de Chimie (2007), 52(11), 1013-1025

Synthesis of imidazol(in)-2-ylidene ruthenium complexes relevant for metathesis and related catalytic processes is described. As an attractive alternative of importance for practical applications, the in ... [more ▼]

Synthesis of imidazol(in)-2-ylidene ruthenium complexes relevant for metathesis and related catalytic processes is described. As an attractive alternative of importance for practical applications, the in situ generation of new NHC ruthenium precatalysts, starting from the easily accessible imidazolium and imidazolinium salts, ruthenium dimer [RuCl2(p-cymene)](2) and a base, is reported in detail. The new NHC family of ruthenium complexes offers great promise as metathesis precatalysts enjoying a wide application profile in organic and polymer synthesis. [less ▲]

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See detailInfantile presentation of the mitochondrial A8344G mutation.
Battisti, Oreste ULg; Scalais, E.; Nuttin, C. et al

in European Journal of Neurology (2007), 14

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See detailL'art de choisir: une question de style
Pâques, Michel ULg

in Annales de Droit de Louvain (2007), (4), 351-354

teaching of law, choice of topics to be taught, how to present them, academic freedom

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See detailDendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma
Henry, E.; Desmet, C. J.; Garzé, V. et al

Poster (2007)

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See detailRole of beta 2-receptors in the anti-inflammatory effects of formoterol in rats with cadmium-induced acute pulmonary inflammation
Zhang, W.; Zhang, F.; Fievez, Laurence ULg et al

in Proceedings: Autumn Meeting of the Belgian Society of Fundamental and Clinical Physiology and Pharmacology (2007)

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See detailPaysages patrimoniaux en Wallonie (Belgique), analyse par approche des paysages témoins
Droeven, Emilie ULg; Dubois, Catherine; Feltz, Claude ULg

in Cahiers d'Economie et Sociologie Rurales (2007), 84-85

In 2000, the Walloon Region (Belgium) has initiated a research program at the Standing Conference on Territorial Development (CPDT) in order to address the European Landscape Convention requirements. The ... [more ▼]

In 2000, the Walloon Region (Belgium) has initiated a research program at the Standing Conference on Territorial Development (CPDT) in order to address the European Landscape Convention requirements. The research concentrated at first on the identification and the typology of Walloon macrolandscapes at a regional scale. Then the research focussed on the landscapes characterisation at a scale of 1/20 000 and their patrimonial qualification. The paper traces the research progress (definition of the concepts of landscape and patrimonial landscape, the adopted procedure) and presents the elaborated method to appreciate the landscapes scientific values, called the method of the “witness landscape”. It also presents the difficulties met during its application on the field. The method is iterative and combines the observation on the field, the review of both historic and present cartographies, the interpretation of aerial photographs and the mobilisation of the available bibliography. Reading, analysing, nderstanding and documenting the landscape in order to highlight the meaningful landscapes’ configurations and components, such was one of the research team contributions to the landscape management. [less ▲]

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See detailL’accompagnement professionnel face aux enjeux de qualité de services
Pirard, Florence ULg

in Brougère, G.; Vandenbroeck, M. (Eds.) Repenser l’éducation des jeunes enfants (2007)

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See detailMitoc-Malu Galben: étude de l'industrie lithique
Otte, Marcel ULg; Noiret, Pierre ULg; Chirica, Vasile et al

in Otte, Marcel; Chirica, Vasile; Haesaerts, Paul (Eds.) L'Aurignacien et le Gravettien de Mitoc-Malu Galben (Moldavie roumaine) (2007)

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See detailTre pastiches montaliani di Fabio Pusterla
Benzoni, Pietro ULg

in Per leggere (2007), 12

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See detailGénération automatique d'une base de données de silhouettes humaines au moyen d'un avatar humain tridimensionnel
Pierard, Sébastien ULg

Master's dissertation (2007)

Pour la conception de systèmes de vidéo-surveillance, il est avantageux d’avoir un système de détection automatique d’êtres humains. Certains de ces systèmes sont basés sur la reconnaissance de la forme ... [more ▼]

Pour la conception de systèmes de vidéo-surveillance, il est avantageux d’avoir un système de détection automatique d’êtres humains. Certains de ces systèmes sont basés sur la reconnaissance de la forme de silhouettes humaines. L’utilisation de méthodes d'apprentissage pour la reconnaissance nécessite une base de données de silhouettes humaines, dont il est intéressant d’automatiser la génération. Dans ce travail, nous utilisons un avatar humain tridimensionnel, celui du projet open-source MakeHuman, afin de générer automatiquement la base de données. Le choix des configurations (positions, orientations et poses) à donner à l’avatar pour obtenir une base de données adéquate est central. Dans un premier temps, nous tentons une étude de l’impact de modifications dans le mode tridimensionnel sur la silhouette. Cet impact est quantifié par la distance de Hausdorff. Parmi les modifications envisagées se trouvent le déplacement, la rotation et le changement de pose de l’avatar. Nous espérions déterminer, à partir de ces impacts, les pas par lesquels doivent varier les différents paramètres. Les configurations que doit prendre l’avatar, pour obtenir une base de données suffisamment riche sans être trop volumineuse, seront obtenues en prenant toutes les combinaisons des valeurs des différents paramètres. Le nombre de configurations est exponentiel en le nombre de degrés de liberté, ce qui rend l’approche inutilisable en pratique, même si nous sommes indifférents à la translation et à la mise à l’échelle des silhouettes. Dans un deuxième temps, nous avons opté pour une approche radicalement différente. Cette fois, nous n’espaçons plus les silhouettes, mais les poses. La position de l’avatar est fixée et il ne peut tourner qu’autour de l’axe vertical. Les poses sont choisies en effectuant des tirages aléatoires des différents paramètres. Cette approche aléatoire du problème a mené à l’élaboration d’un nouveau logiciel, Silhouette. Il est écrit en Java et une interface écrite en C lui permet, grâce à Java Native Interface d’exploiter efficacement le moteur de pose de MakeHuman. Parmi les fonctionnalités de Silhouette, notons la possibilité de restreindre les poses à un ensemble voulu, de ne générer que des silhouettes correspondant à des poses licites et d’annoter les silhouettes. Grâce au logiciel mis en place, nous avons été en mesure de générer diverses bases de données. Nous avons étudié l'impact de l'utilisation de celles-ci sur les performances d'un système de détection de personnes existant. Nous obtenons des résultats encourageants, 73% des silhouettes humaines étant correctement détectées. Un raffinement et un paramétrage soigneux de la méthode devraient mener à des résultats excellents. [less ▲]

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See detailEpithelial-mesenchymal transition process in human embryonic stem cells cultured in feeder-free conditions.
Ullmann, U.; In'T Veld, P.; Gilles, Christine ULg et al

in Molecular Human Reproduction (2007), 13(1), 21-32

Feeder-free human embryonic stem cell (hESC) culture is associated with the presence of mesenchymal-like cells appearing at the periphery of the colonies. The aim of this study was to identify this early ... [more ▼]

Feeder-free human embryonic stem cell (hESC) culture is associated with the presence of mesenchymal-like cells appearing at the periphery of the colonies. The aim of this study was to identify this early differentiation process. Long-term feeder-free hESC cultures using matrigel and conditioned medium from mouse and from human origin revealed that the appearance of mesenchymal-like cells was similar regardless of the conditioned medium used. Standard characterization confirmed the preservation of hESC properties, but the feeder-free cultures could not be maintained longer than 37 passages. The early differentiation process was characterized in the short term after switching hESCs cultured on feeders to feeder-free conditions. Transmission electron microscopy showed an epithelium-like structure inside the hESC colonies, whereas the peripheral cells revealed the acquisition of a rather mesenchymal-like phenotype. Immunochemistry analysis showed that cells at the periphery of the colonies had a negative E-cadherin expression and a positive Vimentin expression, suggesting an epithelial–mesenchymal transition (EMT). Nuclear staining of ß-catenin, positive N-cadherin and negative Connexin 43 expression were also found in the mesenchymal-like cell population. After RT–PCR analysis, Slug and Snail, both EMT-related transcription factors, were detected as up-regulated in the mesenchymal-like cell population. Taken together, our data suggest that culturing hESCs in feeder-free conditions enhances an early differentiation process identified as an EMT. [less ▲]

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