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See detailConformational and thermodynamic changes of the repressor/DNA operator complex upon monomerization shed new light an regulation mechanisms of bacterial resistance against beta-lactam antibiotics
Boudet, J.; Duval, V.; Van Melckebeke, H. et al

in Nucleic Acids Research (2007), 35(13), 4384-4395

In absence of beta-lactam antibiotics, Blal and Mecl homodimeric repressors negatively control the expression of genes involved in P-lactam resistance in Bacillus licheniformis and in Staphylococcus ... [more ▼]

In absence of beta-lactam antibiotics, Blal and Mecl homodimeric repressors negatively control the expression of genes involved in P-lactam resistance in Bacillus licheniformis and in Staphylococcus aureus. Subsequently to P-lactam presence, Blal/Mecl is inactivated by a single-point proteolysis that separates its N-terminal DNA-binding domain to its C-terminal domain responsible for its dimerization. Concomitantly to this proteolysis, the truncated repressor acquires a low affinity for its DNA target that explains the expression of the structural gene for resistance. To understand the loss of the high DNA affinity of the truncated repressor, we have determined the different dissociation constants of the system and solved the solution structure of the B. licheniformis monomeric repressor complexed to the semi-operating sequence OP1, of blaP (1/20P(1)blaP) by using a de novo docking approach based on inter-molecular nuclear Overhauser effects and chemical-shift differences measured on each macromolecular partner. Although the N-terminal domain of the repressor is not subject to internal structural rearrangements upon DNA binding, the molecules adopt a tertiary conformation different from the crystallographic operator-repressor dimer complex, leading to a 300 rotation of the monomer with respect to a central axis extended across the DNA. These results open new insights for the repression and induction mechanisms of bacterial resistance to beta-lactams. [less ▲]

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See detailComplications in living liver donor according to Clavien's classification: An European experience
De Roover, Arnaud ULg; Detry, Olivier ULg; Meurisse, Nicolas ULg et al

in Journal of Hepatology (2007), 46(Suppl. 1), 66

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See detailBelgium : Characteristics of International Administration in Crisis Area
Candela Soriano, Mercedes; Brunet, Sébastien ULg

in Korhonen, Outi (Ed.) International Administration of Crisis Area : Nine National Approaches (2007)

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See detailValidation of a new finite element for incremental forming simulation using a dynamic explicit approach
Henrard, Christophe ULg; Bouffioux, Chantal ULg; Duchene, Laurent ULg et al

in Key Engineering Materials [=KEM] (2007), 344

A new method for modeling the contact between the tool and the metal sheet for the incremental forming process was developed based on a dynamic explicit time integration scheme. The main advantage of this ... [more ▼]

A new method for modeling the contact between the tool and the metal sheet for the incremental forming process was developed based on a dynamic explicit time integration scheme. The main advantage of this method is that it uses the actual contact location instead of fixed positions, e.g. integration or nodal points. The purpose of this article is to compare the efficiency of the new method, as far as accuracy and computation time are concerned, with finite element simulations using a classic static implicit approach. In addition, a sensitivity analysis of the mesh density will show that bigger elements can be used with the new method compared to those used in classic simulations. [less ▲]

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See detailComparison of ammonia and greenhouse gas emissions during the fattening of pigs, kept either on fully slatted floor or on deep litter
Philippe, François-Xavier ULg; Laitat, Martine ULg; Canart, Bernard et al

in Livestock Science (2007), 111(1-2), 144-152

Five successive batches of fattening pigs were raised, each during a four month period, on a totally concrete slatted floor in one experimental room and on straw based deep litter in another. The rooms ... [more ▼]

Five successive batches of fattening pigs were raised, each during a four month period, on a totally concrete slatted floor in one experimental room and on straw based deep litter in another. The rooms were automatically ventilated to maintain a constant ambient temperature. Available floor space was of 0.75 in m(2) per pig kept on the slatted floor and 1.20 m(2) per pig kept on the deep litter. With this last system, about 46 kg of straw were supplied per pig throughout a fattening period. The slurry pit was emptied and the litter removed after each batch. Once a month, the emissions of ammonia (NH3) nitrous oxide (N2O), methane (CH4), carbon dioxide (CO2) and water vapour (H2O) were measured continuously for 6 consecutive days by infra-red. photoacoustic detection. The performance of the animals was not significantly different according to the floor type. Gaseous emissions from pigs raised on the slatted floor and on the deep litter were, respectively, 6.2 and 13.1 g per pig per day for NH3, 0.54 and 1.11 g per pig per day for N2O, 16.3 and 16.0 g per pig per day for CH4, 1.74 and 1.97 kg per pig per day for CO2 and 2.48 and 3.70 kg per pig per day for H2O. Except for the CH4 emissions, all the differences were significant (P<0.001). Thus, pig fattening on deep litter releases nearly 20% more greenhouse gases than on slatted floor, with 2.64 and 2.24 kg of CO2 equivalents, respectively (P<0.001). Whatever the floor type, emissions increased from the beginning to the end of the fattening periods by about 5 times for NH3, 4 times for N2O, 3 times for CH4 and 2 times for CO2 and H2O. Correlation coefficients between CO2-emissions and H2O, NH3 and CH4 emissions were, on average for both floor types, 0.82, 0.77 and 0.74, respectively. Although rearing pigs on straw generally has a good brand image for the consumer, this rearing system produces more pollutant gases than keeping pigs on slatted floors. (c) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailGround-based FTIR measurements at Ile de La Réunion: Observations, error analysis and comparisons with satellite data.
Senten, Cindy; De Mazière, Martine; Hermans, Christian et al

in Geophysical Research Abstracts (2007), 9

Ground-based Fourier-transform infrared (FTIR) spectroscopy is a powerful remote sensing technique to obtain information on the total column abundances and on the vertical distribution of various ... [more ▼]

Ground-based Fourier-transform infrared (FTIR) spectroscopy is a powerful remote sensing technique to obtain information on the total column abundances and on the vertical distribution of various constituents in the atmosphere. Many of these species are essential for the investigation of important atmospheric phenomena, such as the overall greenhouse effect or the stratospheric ozone decrease and recovery. In the frame of the Network for the Detection of Atmospheric Composition Change (NDACC), such observations have been made since many years at several measurement stations for the worldwide long-term monitoring of the atmospheric composition. In this work, we present the results from two short-term FTIR measurement campaigns in 2002 and 2004 at the Ile de La Réunion (21°S, 55°E), a complementary NDACC site in the subtropics, in the Indian Ocean. All spectra were recorded in solar absorption mode. The results discussed here concern the direct greenhouse gases methane (CH4), nitrous oxide (N2O) and ozone (O3), and the indirect greenhouse gases carbon monoxide (CO) and ethane (C2H6), as well as hydrogen cyanide (HCN) and stratospheric hydrogen chloride (HCl), hydrogen fluoride (HF) and nitric acid (HNO3). For the latter species (HCN, HCl, HF and HNO3), we show time series of total column amounts from the surface up to 60 km. For CO, CH4, N2O and O3, it is possible to derive additionally independent information on a few partial columns; these time series are discussed as well. A complete error budget of the retrieval products is given. Temporary mutually correlated enhancements of CO, C2H6and HCN have been observed. They have been traced back to biomass burning events in southern Africa and Madagascar using the FLEXPART model. Comparisons of our retrievals with correlative data from satellite experiments, such as ACE and MOPITT, and with available ozone soundings, show generally good agreements between the different data sets. [less ▲]

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See detailLa copie de la "Cène" de Léonard de Vinci conservée à l'abbaye de Tongerlo
Fagnart, Laure ULg

in Pedretti, Carlo (Ed.) Catalogue de l'exposition Leonardo. The european genius. Paintings and drawings (Bruxelles, Basilique de Koekelberg) (2007)

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See detailCold-adapted enzymes from marine antarctic microorganisms
Marx, J. C.; Collins, T.; D'Amico, Salvino ULg et al

in Marine Biotechnology (2007), 9(3, May-Jun), 293-304

The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the ... [more ▼]

The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the viscosity of the medium, which is also significantly enhanced by low temperatures, contributes to slow down reaction rates. This review analyses the different challenges and focuses on a key element of life at low temperatures: cold-adapted enzymes. The molecular characteristics of these enzymes are discussed as well as the adaptation strategies which can be inferred from the comparison of their properties and three-dimensional structures with those of their mesophilic counterparts. As these enzymes display a high specific activity at low and moderate temperatures associated with a relatively high thermosensitivity, the interest in these properties is discussed with regard to their current and possible applications in biotechnology. [less ▲]

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See detailPolyphase karst system in Cretaceous chalk and calcarenite of the Belgian-Dutch border
Willems, Luc ULg; Rodet, Joël; Fournier, Matthieu et al

in Zeitschrift für Geomorphologie (2007), 51(3), 361-376

Along the Belgian-Dutch border, underground and surface quarries dug in Cretaceous calcarenite and chalk intersect many karst features as well as deep large nodes of weathered rock. Their observation ... [more ▼]

Along the Belgian-Dutch border, underground and surface quarries dug in Cretaceous calcarenite and chalk intersect many karst features as well as deep large nodes of weathered rock. Their observation allows the reconstruction of the genesis of an original karst system resulting from the merging of initially independent endokarsts and exokarsts. Deep weathering has developed within the Cretaceous formations, creating nodes of weathered chalk and closed cavities. These phenomena are expanded over time and can form interconnected voids. Near the surface, solution pipes are generated under the coarsest deposits of a fluvial terrace capping the Cretaceous formations. These pipes develop vertically and may be related to the progressive lowering of the water table in connection with the incision of the Meuse valley. Some of these phenomena cut up the older endokarsts and organize complex systems of out-flow within the chalk. [less ▲]

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See detailRaccontare nel tempo. Narrazione epica e cronologia nelle Argonautiche di Apollonio Rodio
Caneva, Stefano ULg

in Caneva, Stefano; Tarenzi, Victoria (Eds.) Il lavoro sul mito nell’epica greca. Letture di Omero e Apollonio (2007)

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See detailMémoire de travail et vieillissement normal
Collette, Fabienne ULg

in Aubin, Ghislaine; Coyette, Françoise; Pradat-Diehl, P (Eds.) et al Neuropsychologie de la mémoire de travail (2007)

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See detailMise en place d’une session d’interrogations formatives au mois de novembre: impact sur la motivation et les performances ultérieures des étudiants
Mignon, Jacques ULg; Closset, Jean-Louis ULg

in Actes du 24e congrès de l’Association internationale de pédagogie universitaire (AIPU) (2007)

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See detailThe Aznalcóllar disaster: An in-depth PIXE study of the pirite mine spill of 1998
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Álvarez, M. A. et al

Poster (2007)

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See detailAnti-predator defence mechanisms in sawfly larvae of Arge (Hymenoptera, Argidae)
Petre, Charles-Albert ULg; Detrain, Claire; Boevé, Jean-Luc

in Journal of Insect Physiology (2007), 53

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See detailPeut-on lutter contre l'échec scolaire ? (3e éd.)
Crahay, Marcel ULg

Book published by De Boeck (2007)

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See detailCaractérisation de la protéine 9p du virus de la varicelle et du zona (VZV)
Joris-Gerards, Aline; BONTEMS, Sébastien ULg; Di Valentin et al

Poster (2007)

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See detailLa Prévoyance Sociale ou la gestion habile d'une coopérative socialiste
Geerkens, Eric ULg

in Peiren, Luc; Geerkens, Eric; Vincent, Anne (Eds.) et al Cent ans de P&V 1907-2007. L'histoire originale d'une société coopérative d'assurances, vol. 2 (2007)

Les quatre chapitres qui forment cette contribution traitent, suivant un plan chronologique distinguant quatre périodes, des aspects économiques de l'histoire de La Prévoyance Sociale, société coopérative ... [more ▼]

Les quatre chapitres qui forment cette contribution traitent, suivant un plan chronologique distinguant quatre périodes, des aspects économiques de l'histoire de La Prévoyance Sociale, société coopérative d'assurances, fondée en 1907. L'exposé des structures et de l'organisation de la société dans ses différentes composantes — organes dirigeants et organisation générale de l'activité, politique du personnel, de l'administration centrale autant que des inspections, et stratégie de promotion des activités — introduit l'examen du développement de son activité dans ses diverses branches et de sa politique de placement. [less ▲]

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See detailL'éléphant au Ranch de Gibier de Nazinga
Bouché, Philippe ULg

in Delvingt, Willy; Vermeulen, Cédric (Eds.) Nazinga (2007)

Cet article présente l'évolution des tendances des population d'éléphant au Ranch de Gibier de Nazinga ainsi que sa distribution.

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