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See detailPregnancy-Associated Glycoprotein 75 [Fragment] from Bubalus bubalis placenta - Access number P85048
Barbato, O.; Melo de Sousa, Noelita ULg; Debenedetti, A. et al

E-print/Working paper (2007)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailThe systemic resistance induced in tomato by a non-pathogenic Pseudomonas strain is associated with the stimulation of the lipoxygenase pathway
Adam, Akram; Duby, Franceline ULg; Ongena, MARC ULg et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2007), 30

Root treatment by the non-pathogenic Pseudomonas putida strain BTP1 reduced by 34% the disease caused by Botrytis cinerea on tomato leaves. This induced systemic resistance phenomenon is associated both ... [more ▼]

Root treatment by the non-pathogenic Pseudomonas putida strain BTP1 reduced by 34% the disease caused by Botrytis cinerea on tomato leaves. This induced systemic resistance phenomenon is associated both with the accumulation of fungitoxic material and with the stimulation of the lipoxygenase pathway in infected leaves. More precisely, we observed a consistent change in the expression of a new tomloxF gene in the leaves from BTP1-treated plants as far as the pathogen is introduced. This suggests that the roots were primed and reacted locally to colonization by bacteria and that defense-related gene expression is turned on systemically upon pathogen perception [less ▲]

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

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See detailEvolution de l'utilisation des intrants en céréaliculture : le cas des produits de protection des cultures
Dogot, Thomas ULg

in CRA-w; FUSAGx (Eds.) 40 ans Livre blanc Céréales-Gembloux "Economie de la production céréalière en Belgique : évolution en 40 ans de Politique Agricole Commune et perspectives d’avenir" (2007)

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See detailLe syndrome Piro-like chez le cheval
Sandersen, Charlotte ULg; Pittel, PH; Amory, Hélène ULg

in Proceedins of the 24th Annual Congress of the Belgian Equine Practitioners Society (BEPS) (2007)

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See detailPompe externe à perfusion continue d'insuline et lipodystrophies
RADERMECKER, Régis ULg

in Infusystemes France (2007), 24(2), 13-16

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See detailThermal and structural behavior of blends made of palm oil, anhydrous milk fat or their fractions.
Braipson, Sabine ULg; Gibon, Véronique; Deroanne, Claude

Conference (2007)

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See detailConformational and thermodynamic changes of the repressor/DNA operator complex upon monomerization shed new light an regulation mechanisms of bacterial resistance against beta-lactam antibiotics
Boudet, J.; Duval, V.; Van Melckebeke, H. et al

in Nucleic Acids Research (2007), 35(13), 4384-4395

In absence of beta-lactam antibiotics, Blal and Mecl homodimeric repressors negatively control the expression of genes involved in P-lactam resistance in Bacillus licheniformis and in Staphylococcus ... [more ▼]

In absence of beta-lactam antibiotics, Blal and Mecl homodimeric repressors negatively control the expression of genes involved in P-lactam resistance in Bacillus licheniformis and in Staphylococcus aureus. Subsequently to P-lactam presence, Blal/Mecl is inactivated by a single-point proteolysis that separates its N-terminal DNA-binding domain to its C-terminal domain responsible for its dimerization. Concomitantly to this proteolysis, the truncated repressor acquires a low affinity for its DNA target that explains the expression of the structural gene for resistance. To understand the loss of the high DNA affinity of the truncated repressor, we have determined the different dissociation constants of the system and solved the solution structure of the B. licheniformis monomeric repressor complexed to the semi-operating sequence OP1, of blaP (1/20P(1)blaP) by using a de novo docking approach based on inter-molecular nuclear Overhauser effects and chemical-shift differences measured on each macromolecular partner. Although the N-terminal domain of the repressor is not subject to internal structural rearrangements upon DNA binding, the molecules adopt a tertiary conformation different from the crystallographic operator-repressor dimer complex, leading to a 300 rotation of the monomer with respect to a central axis extended across the DNA. These results open new insights for the repression and induction mechanisms of bacterial resistance to beta-lactams. [less ▲]

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See detailComplications in living liver donor according to Clavien's classification: An European experience
De Roover, Arnaud ULg; Detry, Olivier ULg; Meurisse, Nicolas ULg et al

in Journal of Hepatology (2007), 46(Suppl. 1), 66

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See detailImprovement in HSS grade for early stands of hot strip mills - Metallurgical features and mechanical properties assessment
Ernst, Roger; Breyer, Jean-Pierre; Tchuindjang, Jérôme Tchoufack ULg et al

in Proceedings of the 43rd Seminar of Rollings (2007)

Aurora and Kosmos grades are HSS alloys belonging to the complex Fe-Cr-C-X system, where X is a strong carbide former element of the V, Mo or W type. Both alloys were metallurgically characterised prior ... [more ▼]

Aurora and Kosmos grades are HSS alloys belonging to the complex Fe-Cr-C-X system, where X is a strong carbide former element of the V, Mo or W type. Both alloys were metallurgically characterised prior to their comparison. Metallurgical analyses involved phases identification and carbides quantification by using Scanning Electron Microscopy and Energy Dispersive X rays. Differential Thermal Analysis was performed to allow a better understanding of the solidification sequence of studied alloys while mechanical tests performed were compressive at room temperature and bulk hardness at usual service temperatures. An attempt was made in order to connect experimental results to the good behaviour in operation of Aurora grade. In fact Aurora grade appeared to exhibit strong metallurgical differences when compared to Kosmos grade, especially as concern in nature and amount of carbides. [less ▲]

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See detailValidation of a new finite element for incremental forming simulation using a dynamic explicit approach
Henrard, Christophe ULg; Bouffioux, Chantal ULg; Duchene, Laurent ULg et al

in Key Engineering Materials [=KEM] (2007), 344

A new method for modeling the contact between the tool and the metal sheet for the incremental forming process was developed based on a dynamic explicit time integration scheme. The main advantage of this ... [more ▼]

A new method for modeling the contact between the tool and the metal sheet for the incremental forming process was developed based on a dynamic explicit time integration scheme. The main advantage of this method is that it uses the actual contact location instead of fixed positions, e.g. integration or nodal points. The purpose of this article is to compare the efficiency of the new method, as far as accuracy and computation time are concerned, with finite element simulations using a classic static implicit approach. In addition, a sensitivity analysis of the mesh density will show that bigger elements can be used with the new method compared to those used in classic simulations. [less ▲]

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See detailInvestigation of the preparation and activity of gold catalysts in the total oxidation of n-hexane
Cellier, Caroline; Lambert, Stéphanie ULg; Gaigneaux, Eric M. et al

in Applied Catalysis B : Environmental (2007), 70(1-4), 406-416

The factors affecting the preparation of Au/TiO2 catalysts and their activity in the total oxidation of n-hexane were investigated. The mechanism of gold deposition-precipitation is discussed through ... [more ▼]

The factors affecting the preparation of Au/TiO2 catalysts and their activity in the total oxidation of n-hexane were investigated. The mechanism of gold deposition-precipitation is discussed through comparison of the samples prepared by this method and others prepared by anion adsorption method. The influence of the pH and of the origin of TiO2 support used are additionally addressed. The difference of gold dispersion observed between the two methods is attributed to a difference of mobility of the gold precursors during the thermal treatment rather than to a difference of dispersion over the uncalcined samples. The mechanism of gold deposition-precipitation actually involves the reactions of gold hydroxy-chloride species with the surface. Another pan of the work, thus, concerned the use of the deposition-precipitation method to prepare a Au/MnO2 catalyst. It is shown that the activity of gamma-MnO2 is directly proportional to its surface area and that the deposition-precipitation procedure decreases the surface and activity of MnO2. However, the deposition of gold allows to avoid a too deep sintering of gamma-MnO2 and, thus, helps to somehow preserve its activity. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailIntrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells
Verhaeghe, Catherine ULg; Tabruyn, Sébastien ULg; Oury, Cécile ULg et al

in Biochemical and Biophysical Research Communications (2007), 356(3), 745-749

Cystic fibrosis is a common genetic disorder characterized by a severe lung inflammation and fibrosis leading to the patient's death. Enhanced angiogenesis in cystic fibrosis (CF) tissue has been ... [more ▼]

Cystic fibrosis is a common genetic disorder characterized by a severe lung inflammation and fibrosis leading to the patient's death. Enhanced angiogenesis in cystic fibrosis (CF) tissue has been suggested, probably caused by the process of inflammation, as similarly described in asthma and chronic bronchitis. The present study demonstrates an intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells. Microarray experiments showed that CF airway epithelial cells expressed several angiogenic factors such as VEGF-A, VEGF-C, bFGF, and PLGF at higher levels than control cells. These data were confirmed by real-time quantitative PCR and, at the protein level, by ELISA. Conditioned media of these cystic fibrosis cells were able to induce proliferation, migration and sprouting of cultured primary endothelial cells. This report describes for the first time that cystic fibrosis epithelial cells have an intrinsic angiogenic activity. Since excess of angiogenesis is correlated with more severe pulmonary disease, our results could lead to the development of new therapeutic applications. (c) 2007 Elsevier Inc. All rights reserved. [less ▲]

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See detailBioéthique
Palermini, Patricia ULg

in Durand, Pascal (Ed.) Les Nouveaux Mots du Pouvoir. Abécédaire critique (2007)

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See detailCold-adapted enzymes from marine antarctic microorganisms
Marx, J. C.; Collins, T.; D'Amico, Salvino ULg et al

in Marine Biotechnology (2007), 9(3, May-Jun), 293-304

The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the ... [more ▼]

The Antarctic marine environment is characterized by challenging conditions for the survival of native microorganisms. Indeed, next to the temperature effect represented by the Arrhenius law, the viscosity of the medium, which is also significantly enhanced by low temperatures, contributes to slow down reaction rates. This review analyses the different challenges and focuses on a key element of life at low temperatures: cold-adapted enzymes. The molecular characteristics of these enzymes are discussed as well as the adaptation strategies which can be inferred from the comparison of their properties and three-dimensional structures with those of their mesophilic counterparts. As these enzymes display a high specific activity at low and moderate temperatures associated with a relatively high thermosensitivity, the interest in these properties is discussed with regard to their current and possible applications in biotechnology. [less ▲]

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See detailLevel of S100B protein expression in the amnion at various gestational ages in the third trimester of normal pregnancies.
Tskitishvili, Ekaterine ULg; Komoto, Yoshiko; Kinugasa, Yukiko et al

in Acta Obstetricia et Gynecologica Scandinavica (2007), 86(8), 915-20

BACKGROUND: S100B protein is a unique calcium-binding protein. Its biological role within the cell populations is not completely defined. Some pathological conditions that develop during pregnancy could ... [more ▼]

BACKGROUND: S100B protein is a unique calcium-binding protein. Its biological role within the cell populations is not completely defined. Some pathological conditions that develop during pregnancy could affect S100B concentrations in the amniotic fluid, cord blood, and maternal serum. The aim of our study was to assess the correlation between S100B protein expression in the amnion, amniotic fluid and gestational age in the third trimester of uncomplicated pregnancies. METHODS: Amnion, amniotic fluid, maternal peripheral and umbilical cord blood samples were collected from healthy women who delivered at 31-36 weeks (n=17), 37-40 weeks (n=22), and 41-42 weeks (n=21). The expression of S100B in the amnion was assessed by immunohistochemistry and real-time (RT)-PCR, and its concentrations in amniotic fluid, maternal and cord blood sera were determined by ELISA. RESULTS: The S100B protein expression in the amnion and its concentrations in amniotic fluid, maternal and cord blood sera of patients in the third trimester were not significantly different at various gestational ages. CONCLUSIONS: The S100B protein expression in the amnion and the S100B protein concentrations in amniotic fluid, maternal and cord blood do not vary significantly in the third trimester of uncomplicated pregnancies. [less ▲]

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See detailPublic Pension Reform: A Primer
Jousten, Alain ULg

E-print/Working paper (2007)

The present paper reviews key issues in pension design and pension reform encountered all <br />across the world. The paper heavily refers to the recent U.S. Social Security reform debate in <br />general ... [more ▼]

The present paper reviews key issues in pension design and pension reform encountered all <br />across the world. The paper heavily refers to the recent U.S. Social Security reform debate in <br />general and to the Personal Retirement Accounts proposal in particular. A particular <br />emphasis is put on annuitization and risk-taking in the economy. Our discussion signals some <br />inadequacy of the proposed measures with respect to the goals of viability of the system and <br />individual financial security during retirement. [less ▲]

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See detailApplication of Laser Induced Breakdown Spectroscopy (LIBS) to the determination of transition probabilities of laser lines in Au II spectrum
Ortiz, M.; Mayo, R.; Biémont, Emile ULg et al

Conference (2007)

Experimental branching fractions for laser lines arising from excited states of 5d97s configuration of Au II have been determined by application of Laser Induced Breakdown Spectroscopy (LIBS). The ... [more ▼]

Experimental branching fractions for laser lines arising from excited states of 5d97s configuration of Au II have been determined by application of Laser Induced Breakdown Spectroscopy (LIBS). The experimental relative transition probabilities were converted into an absolute scale using theoretical values for the radiative lifetimes of the corresponding states, calculated in this work. Transition probabilities and radiative lifetimes have been determined by a relativistic Hartree - Fock method taking configuration interaction and core-polarization effects into account. A comparison of the present results with the available theoretical values has been made and shows a reasonable agreement although discrepancies are observed between theory and experiment for some transitions. [less ▲]

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