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See detailLactation effect on the trace element dynamics in northern elephant seals (Mirounga angustirostris)
Habran, Sarah ULg; Debier, Cathy; Lepoint, Gilles ULg et al

Conference (2007, November 30)

Northern elephant seals, Mirounga angustirostris, are exceptional mammals: females fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Females thus lose ... [more ▼]

Northern elephant seals, Mirounga angustirostris, are exceptional mammals: females fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Females thus lose approximately a third of body mass and produce energy-rich milk with a high fat content. Stable carbon and nitrogen isotope ratios (d13C and d15N used as diet markers) and total mercury concentrations (Hg) were measured in tissues (blood cells, serum, full blood, milk and blubber) from 20 mother-pup pairs on days 4 and 21 of lactation. A systematic difference was observed between isotopic values in blood cells and serum of the mothers, linked to diet and distinct biochemical composition between blood components. d13C differed between inner and outer blubber layers and were similar between inner blubber and milk. High Hg concentrations (up to 350 ng.g-1ww at the beginning of lactation) were found in the blood of mother elephant seals linked to their high d15N values in blood cells. Hg transfer through placenta and milk was observed between mothers and pups. A significant increase of blood Hg concentration in mothers (+285 ng.g-1ww) and a decrease in pups (-93 ng.g-1ww) were observed between days 4 and 21 of lactation. Both processes were explained by a remobilization of proteins and lipids during fasting and milk production in mothers and by a dilution of Hg in pups due to their high mass increase during this period. This study confirms that lactation modifies stable nitrogen isotope ratios in tissues, as well as mercury levels in blood from mothers and pups, and highlights the existence of a transplacental and transmammary transfer of mercury in northern elephant seals. Therefore, physiological processes and body condition should be considered carefully when interpreting stable isotope ratios and Hg concentrations in the framework of biomonitoring. [less ▲]

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See detailNew insights into peripherin expression in cochlear neurons
Lallemend, François; Vandenbosch, Renaud ULg; Hadjab, S. et al

in Neuroscience (2007), 150(1), 212-222

Peripherin is an intermediate filament protein that is expressed in peripheral and enteric neurons. In the cochlear nervous system, peripherin expression has been extensively used as a differentiation ... [more ▼]

Peripherin is an intermediate filament protein that is expressed in peripheral and enteric neurons. In the cochlear nervous system, peripherin expression has been extensively used as a differentiation marker by preferentially labeling the type II neuronal population at adulthood, but yet without knowing its function. Since the expression of peripherin has been associated in time with the process of axonal extension and during regeneration of nerve fibers in other systems, it was of interest to determine whether peripherin expression in cochlear neurons was a static phenotypic trait or rather prone to modifications following nerve injury. In the present study, we first compared the expression pattern of peripherin and beta III-tubulin from late embryonic stages to the adult in rat cochlea. The staining for both proteins was seen before birth within all cochlear neurons. By birth, and for 2 or 3 days, peripherin expression was gradually restricted to the type II neuronal population and their projections. In contrast, from postnatal day (P) 10 onwards, while the expression of beta III-tubulin was still found in projections of all cochlear neurons, only the type I population had beta III-tubulin immunoreactivity in their cell bodies. We next investigated the expression of peripherin in axotomized cochlear neurons using an organotypic explant model. Peripherin expression was surprisingly re-expressed in a vast majority of neurons after axotomy. In parallel, the expression and localization of beta III-tubulin and peripherin in dissociated cultures of cochlear neurons were studied. Both proteins were distributed along the entire neuronal length but exhibited complementary distribution, especially within the projections. Moreover, peripherin immunoreactivity was still abundant in the growth cone, whereas that of beta III-tubulin was decreasing at this compartment. Our findings are consistent with a model in which peripherin plays an important structural role in cochlear neurons and their projections during both development and regenerative processes and which is compatible with the assumption that frequently developmentally regulated factors are reactivated during neuronal regeneration. [less ▲]

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See detailLes peintures du bâti dans les traités d’architecture
Gilles, Isabelle ULg

Scientific conference (2007, November 29)

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See detailThe Definition of the Concept of Tax
Bourgeois, Marc ULg

Conference (2007, November 29)

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See detailIsolation and identification of inulooligosaccharides resulting from inulin hydrolysis
Ronkart, Sebastien N; Blecker, Christophe ULg; Fourmanoir, Hélène et al

in Analytica Chimica Acta (2007), 604(1), 81-87

In this study, inulooligosaccharides (F-n-type inulin) resulting from the endo-inulinase hydrolysis of globe artichoke inulin were purified and characterized. The aim was to produce F-n oligomer standards ... [more ▼]

In this study, inulooligosaccharides (F-n-type inulin) resulting from the endo-inulinase hydrolysis of globe artichoke inulin were purified and characterized. The aim was to produce F-n oligomer standards with the intention of identifying them in the complex inulin chromatogram. Inulin was extracted from globe artichoke and presented a high average degree of polymerization (DP) of about 80 as determined by high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). This inulin was hydrolyzed by a commercial endo-inulinase yielding a product with a very high F-n/GF(n), molecule ratio, thus limiting the interference of GF(n) during the purification process. High performance size exclusion chromatography was used to individually isolate and collect each retention peak corresponding to a specific oligomer. The purity of these fractions was checked by HPAEC-PAD and showed that relatively pure molecules were produced. Electrospray ionization mass spectrometry allowed the molecular weight determination of these purified oligomers and ascertained their DP as F-2, F-3 and F-4. These F2-4 standards were used with glucose, fructose, sucrose and GF(2-4) (commercially available) to spike commercial oligofructose products in order to determine the elution profile in the HPAEC-PAD chromatogram. [less ▲]

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See detailCombination of electrografting and layer-by-layer deposition: an efficient way to tailor polymer coatings of (semi)-conductors
Charlot, Aurélia; Gabriel, Sabine ULg; Detrembleur, Christophe ULg et al

in Chemical Communications (2007), (44), 4656-4658

This communication reports on a novel, simple and highly versatile concept, which consists in combining the advantages of two complementary and relevant techniques (i) electrografting and (ii) layer-by ... [more ▼]

This communication reports on a novel, simple and highly versatile concept, which consists in combining the advantages of two complementary and relevant techniques (i) electrografting and (ii) layer-by-layer deposition process with the goal to tailor strongly adhering coatings to (semi)-conducting surfaces imparting them with tunable specific properties. [less ▲]

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See detailPierre Bourdieu : portrait du sociologue en philosophe
Bolmain, Thomas ULg

Conference given outside the academic context (2007)

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See detailA dynamic model of an experimental bloom of coccolithophores Emiliania huxleyi
Joassin, Pascal ULg; Borges, Alberto ULg; Chou, Lei et al

Conference (2007, November 27)

A dynamic model has been developed to represent biogeochemical events observed during an experimentally induced bloom of coccolithophores Emiliania huxleyi. This bloom occurred in a mesocosm experiment ... [more ▼]

A dynamic model has been developed to represent biogeochemical events observed during an experimentally induced bloom of coccolithophores Emiliania huxleyi. This bloom occurred in a mesocosm experiment (Bergen 2001 experiment) during which ecosystem development was followed over a 23-days period through changes of the stocks of inorganic nutrients (nitrate, ammonium and phosphate), dissolved inorganic carbon and pCO2, O2 concentration, pigments, particulate organic carbon and nitrogen, dissolved organic carbon, the production of Transparent Exopolymeric Particles (TEP), primary production, alkalinity, calcification and particulate inorganic carbon. The dynamic model is based on unbalanced algal growth and balanced growth for bacteria as described in Van den Meersche et al. (2004). In addition, in order to adequately reproduce the observations, the model has been extended by including an explicit description of calcification, T.E.P production and an enhanced mortality due to viruses. This last process, based on a critical promiscuity between cellular hosts and viral agents, successfully contributed to reproduce the bloom extinction as observed in the mesocosm experiment. This model will be implemented in a coupled physical-biogeochemical model of the Black Sea ecosystem in the framework of the EU Sesame project and in the Gulf of Biscay in the frame of the Belgian PEACE project. [less ▲]

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See detailPromouvoir des relations interculturelles cohésives dans les villes européennes.
Martiniello, Marco ULg

Scientific conference (2007, November 27)

Detailed reference viewed: 7 (0 ULg)
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See detailLateral-torsional buckling of Stainless steel I-beams in case of fire
Vila Real, Paulo; Lopes, Nuno; Da Silva, Luis et al

Conference (2007, November 27)

Detailed reference viewed: 14 (0 ULg)
See detailEtude d'un cas d'aphasie progressive primaire
Adam, Stéphane ULg

Scientific conference (2007, November 27)

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See detailPrinciples of European Contract Law and Consumer Construction Law
Kohl, Benoît ULg

Conference (2007, November 27)

Detailed reference viewed: 15 (0 ULg)
See detailPromouvoir des relations interculturelles cohésives dans les villes européennes.
Martiniello, Marco ULg

Scientific conference (2007, November 27)

Detailed reference viewed: 4 (0 ULg)
See detailPolitique d’intégration et citoyenneté multiculturelle dans l’Union Européenne.
Martiniello, Marco ULg

Scientific conference (2007, November 27)

Detailed reference viewed: 7 (0 ULg)