References of "2006"
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See detailStrenuous concentric and eccentric isokinetic exercises: specific fatigue patterns ?
Delvaux, François ULg; Bury, Thierry ULg; Lehance, Cédric ULg et al

in Abstract Book “Exercise and muscle physiology” de l’Ecole Doctorale en Sciences de la Motricité (2006)

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See detailMigrants’Citizenship: Legal Status, Rights and Political Participation
Martiniello, Marco ULg; Bauböck, Rainer; Kraler, Albert et al

in Penninx, Rinus; Berger, Maria; Kraal, Karen (Eds.) The Dynamics of International Migration and Settlement in Europe. A State of the Art (2006)

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See detailAnalysis of Ser-His-Glu triad in alpha-amylases
Marx, Jean Claude; Feller, Georges ULg

Poster (2006)

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See detailRecombinant bovine herpesvirus 4 (BoHV-4) expressing glycoprotein D of BoHV-1 is immunogenic and elicits serum-neutralizing antibodies against BoHV-1 in a rabbit model.
Donofrio, Gaetano; Cavirani, Sandro; Vanderplasschen, Alain ULg et al

in Clinical and Vaccine Immunology (2006), 13(11), 1246-54

Several biological characteristics of bovine herpesvirus 4 (BoHV-4) make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity ... [more ▼]

Several biological characteristics of bovine herpesvirus 4 (BoHV-4) make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types coming from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. Starting from BoHV-4 cloned as a bacterial artificial chromosome (BAC), we used MuA transposase-mediated in vitro transposition to generate recombinant BoHV-4 expressing the immunodominant glycoprotein D (gD) of BoHV-1, one of the most important pathogens of cattle. Although a cis-acting element from woodchuck hepatitis virus (the woodchuck hepatitis virus posttranscriptional regulatory element [WPRE]) in the 3' end of the gD expression cassette was required for maximal gD expression from plasmids in transient transfection assays, this element was not necessary for efficient expression of gD from recombinant BoHV-4 genomes. BoHV-4 recombinants containing gD expression cassettes with or without the WPRE expressed gD at similarly high levels. Several cell lines originating from different animal species expressed gD when infected with BoHV-4 recombinants. When rabbits were immunized with one of the recombinants, high levels of serum neutralizing antibodies against BoHV-1 were generated. This work is one of the first demonstrations of the use BoHV-4 as a vector for vaccine purposes and may provide the basis for BoHV-1 vaccination of cattle with recombinant BoHV-4. [less ▲]

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See detailDNA in chromatin: from genome-wide sequence analysis to the modelling of replication in mammals
Arbeodo, Alain; d'Aubenton-Carafa, Y.; Audit, B. et al

in Advances in Chemical Physics (2006), 135

Understanding how chromatin is spatially and dynamically organized in the nucleus of eukaryotic cells and how this affects genome functions is one of the main challenges of cell biology. In that context ... [more ▼]

Understanding how chromatin is spatially and dynamically organized in the nucleus of eukaryotic cells and how this affects genome functions is one of the main challenges of cell biology. In that context the role of the DNA sequence itself in these condensation- decondensation processes is still debated. In this chapter, we explore large-scale nucleotide compositional fluctuations along the human genome through the optics of the wavelet transform microscope. Analysis of the GC content and of the TA and GC skews re- veals the existence of rhythms with characteristic fundamental frequencies that enlighten a remarkable cooperative organization of gene location and orientation. We describe a multi-scale methodology that allows us to predict 1012 replication origins in the 22 hu- man autosomal chromosomes. We present a model of replication with well-positioned replication origins and random termination sites that accounts for the highly relaxational nature of the oscillations observed in the skew profiles. We emphasize these putative replication initiation zones as regions where the chromatin fiber is likely to be more open so that DNA be more easily accessible. We show that, in the crowded environment of the cell nucleus, the presence of these intrinsic decondensed structural defects actually pre- disposes the fiber to spontaneously form multi-looped rosette-like structures that provide an attractive description of genome organization into replication foci that are observed in interphase mammalian nuclei as stable autonomous chromatin domains favoring com- partmentalized DNA replication and gene expression. New experimental perspectives are discussed. [less ▲]

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See detailPotential Estimation of Fatty Acid Content in Cow Milk by Mid-Infrared Spectrometry
Soyeurt, Hélène ULg; Dardenne, Pierre; Lognay, Georges ULg et al

in Prooceedings of the 35th ICAR session (2006)

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See detailElectroantennogram of Culex quinquefasciatus Say (Diptera:Culicidae) to chemical compounds from fermented infusions and improvement of gravid mosquito trapping
Latte, Nicolas ULg

Master's dissertation (2006)

The urban mosquito Culex quinquefasciatus Say (Diptera: Culicidae) uses chemicals to locate suitable water pools for oviposition. These chemicals when identified can be used to trap gravid female ... [more ▼]

The urban mosquito Culex quinquefasciatus Say (Diptera: Culicidae) uses chemicals to locate suitable water pools for oviposition. These chemicals when identified can be used to trap gravid female mosquitoes. Experiments in Davis and in Sacramento (California, in the United States) were made to improve the trapping efficiency of Reiter/Cumming gravid traps baited by several attractants (or attractant blends). Unfortunately, it appeared that the number of gravid female mosquitoes collected was not sufficient to differentiate the efficacy of attractant blends tested by statistical analysis. However, a positive correlation between the total mosquito number collected daily and the highest humidity shows that the mosquito oviposition activity seems to be influenced by the humidity during the night. Observations on total number of mosquitoes and the highest temperature seem to reveal a threshold temperature which is critical for the adult mosquito survival. A coupled gas chromatography – electronantennography (GC-EAD) protocol was also developed step by step in order to find new Culex quinquefasciatus oviposition attractants. After obtaining repetitive electroantennographic (EA) response, two comparison experiments (SPME fiber type and infusion type) were made to finalize the specific GC-EAD/SPME protocol. This with the aim to prospect new attractants from fermented Bermuda grass infusion. Analyses based on all results permit to distinguish the EA responses from random perturbations (electromagnetic and vibration). GC-EAD/SPME of Bermuda grass infusion analyses confirms the presence of 10 and the activity of 9 chemical compounds as established by Du and Millar in 1999. Furthermore, experiments on Culex quinquefasciatus performed with the new protocol, brought to light the EA activity of 10 other unknown chemicals, 7 of which are low molecular weight chemical compounds. These new chemicals have now to be identified and their ability to elicit EA responses still has to be confirmed. [less ▲]

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See detailMise à forme des matériaux - Première partie
Debongnie, Jean-François ULg

Learning material (2006)

Introduction. Irons metallurgy and alloys classifications (DIN, AFNOR, SAE). Casting practice. Forging. Sheet-metal working. Welding, soldering and oxygen cutting. Polder metallurgy. Unconventional ... [more ▼]

Introduction. Irons metallurgy and alloys classifications (DIN, AFNOR, SAE). Casting practice. Forging. Sheet-metal working. Welding, soldering and oxygen cutting. Polder metallurgy. Unconventional machining processes. [less ▲]

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See detailCrystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding models.
Borrego-Diaz, Emma; Kerff, Frédéric ULg; Lee, Sung Haeng et al

in Journal of Structural Biology (2006), 155(2), 230-8

Alpha-actinin belongs to the spectrin family of actin crosslinking and bundling proteins that function as key regulators of cell motility, morphology and adhesion. The actin-binding domain (ABD) of these ... [more ▼]

Alpha-actinin belongs to the spectrin family of actin crosslinking and bundling proteins that function as key regulators of cell motility, morphology and adhesion. The actin-binding domain (ABD) of these proteins consists of two consecutive calponin homology (CH) domains. Electron microscopy studies on ABDs appear to support two competing actin-binding models, extended and compact, whereas the crystal structures typically display a compact conformation. We have determined the 1.7A resolution structure of the ABD of alpha-actinin 1, a ubiquitously expressed isoform. The structure displays the classical compact conformation. We evaluated the two binding models by surface conservation analysis. The results show a conserved surface that spans both domains and corresponds to two previously identified actin-binding sites (ABS2 and ABS3). A third, and probably less important site, ABS1, is mostly buried in the compact conformation. However, a thorough examination of existing structures suggests a weak and semi-polar binding interface between the two CHs, leaving open the possibility of domain reorientation or opening. Our results are consistent with a two-step binding mechanism in which the ABD interacts first in the compact form observed in the structures, and then transitions toward a higher affinity state, possibly through minor rearrangement of the domains. [less ▲]

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See detailBiotechnological approaches to cassava protein improvement
Stupak, M.; Vanderschuren, Hervé ULg; Gruissem, W. et al

in Trends in Food Science & Technology (2006), 17(12), 634-641

Cassava starchy storage roots are an excellent source of carbohydrates but lacking in protein. To enhance their nutritional quality, here we discuss several biotechnological strategies that might be used ... [more ▼]

Cassava starchy storage roots are an excellent source of carbohydrates but lacking in protein. To enhance their nutritional quality, here we discuss several biotechnological strategies that might be used to increase protein levels as well as improved essential amino acid content in transgenic cassava. Application of such strategies in this major crop could, in the long term, help to fight against malnutrition in those regions which depend heavily on cassava consumption. © 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailNon-genetic factors affecting the reproduction performance, lamb growth and productivity indices of Djallonke sheep
Gbangboche, A. B.; Adamou-Ndiaye, M.; Youssao, A. K. I. et al

in Small Ruminant Research (2006), 64((1-2)), 133-142

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See detailLC method for the determination of R-timolol in S-timolol maleate: Validation of its ability to quantify and uncertainty assessment.
Marini Djang'Eing'A, Roland ULg; Chiap, Patrice ULg; Boulanger, Bruno ULg et al

in Talanta (2006), 68(4), 1166-1175

This article presents the validation results of a chiral liquid chromatographic (LC) method previously developed for the quantitative determination of R-timolol in S-timolol maleate samples. A novel ... [more ▼]

This article presents the validation results of a chiral liquid chromatographic (LC) method previously developed for the quantitative determination of R-timolol in S-timolol maleate samples. A novel validation strategy based on the accuracy profiles was used to select the most appropriate regression model, to assess the method accuracy within well defined acceptance limits and to determine the limits of quantitation as well as the concentration range. The validation phase was completed by the investigation of the risk profiles of various acceptable regression models in order to ensure the risk of obtaining the future measurements outside the acceptance limits fixed a priori. On the other hand, the present paper also shows how data used in this validation approach can be used to estimate the measurement uncertainty. The uncertainty derived from beta-expectation tolerance interval (sigma(Tol)(2)), which is equal to the uncertainty of measurements as well as the expanded uncertainty (U(x)) using a coverage factor k=2 was estimated. The uncertainty estimates obtained from validation data were finally compared with those obtained from interlaboratory and robustness studies. [less ▲]

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See detailDetection of novel single-nucleotide polymorphisms (SNPs) in the CYP21 gene and association analysis of two SNPs for CYP21 and ESR2 with litter size in a commercial sow population
Buske, Bernd ULg; Sternstein, Ina; Reissmann, Monika et al

in Journal of Animal Breeding & Genetics (2006), 123(5), 343-348

Detailed reference viewed: 29 (11 ULg)