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See detailS100B protein expression in the amnion and amniotic fluid in pregnancies complicated by pre-eclampsia.
Tskitishvili, Ekaterine ULg; Komoto, Yoshiko; Temma-Asano, Kumiko et al

in Molecular Human Reproduction (2006), 12(12), 755-61

Our aim was to investigate the expression of S100B protein in the amnion and to assess the amniotic fluid concentration in pregnancies complicated by pre-eclampsia. Samples were obtained from women who ... [more ▼]

Our aim was to investigate the expression of S100B protein in the amnion and to assess the amniotic fluid concentration in pregnancies complicated by pre-eclampsia. Samples were obtained from women who developed pre-eclampsia (n = 7), pre-eclampsia with intrauterine growth retardation (IUGR) (n = 4), normotensive IUGR (n = 7) and gestational hypertension (n = 4) during pregnancy and healthy controls who delivered at term (n = 35). To determine the difference in the expression of S100B in the amnion, we performed immunohistochemistry, western blot analysis and RT-PCR. Using enzyme-linked immunosorbent assay (ELISA), we assessed the S100B concentration in amniotic fluid. The S100B mRNA expression in the amnion of pre-eclamptic patients and patients with pre-eclampsia with IUGR was significantly higher than that in the control. The amniotic fluid S100B protein concentration of the pre-eclampsia and normotensive IUGR cases was significantly higher than that of the control. This study shows that amnion could be a source responsible for the increased concentration of S100B in amniotic fluid. In pre-eclampsia, reactive oxygen species (ROS) are generated by oxidative stress. Some pathological conditions that develop during pregnancy and are related to hypoxic stress can affect the elevation of S100B concentration in the amnion. [less ▲]

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See detailLes Fonts de Liège. De l’art antique à l’art mosan : sources iconographiques et stylistiques
George, Philippe ULg

in Halleux, Robert; Xhayet, Geneviève (Eds.) Etudes sur les fonts baptismaux de Saint-Barthélemy à Liège, (2006)

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See detailDNA in chromatin: from genome-wide sequence analysis to the modelling of replication in mammals
Arbeodo, Alain; d'Aubenton-Carafa, Y.; Audit, B. et al

in Advances in Chemical Physics (2006), 135

Understanding how chromatin is spatially and dynamically organized in the nucleus of eukaryotic cells and how this affects genome functions is one of the main challenges of cell biology. In that context ... [more ▼]

Understanding how chromatin is spatially and dynamically organized in the nucleus of eukaryotic cells and how this affects genome functions is one of the main challenges of cell biology. In that context the role of the DNA sequence itself in these condensation- decondensation processes is still debated. In this chapter, we explore large-scale nucleotide compositional fluctuations along the human genome through the optics of the wavelet transform microscope. Analysis of the GC content and of the TA and GC skews re- veals the existence of rhythms with characteristic fundamental frequencies that enlighten a remarkable cooperative organization of gene location and orientation. We describe a multi-scale methodology that allows us to predict 1012 replication origins in the 22 hu- man autosomal chromosomes. We present a model of replication with well-positioned replication origins and random termination sites that accounts for the highly relaxational nature of the oscillations observed in the skew profiles. We emphasize these putative replication initiation zones as regions where the chromatin fiber is likely to be more open so that DNA be more easily accessible. We show that, in the crowded environment of the cell nucleus, the presence of these intrinsic decondensed structural defects actually pre- disposes the fiber to spontaneously form multi-looped rosette-like structures that provide an attractive description of genome organization into replication foci that are observed in interphase mammalian nuclei as stable autonomous chromatin domains favoring com- partmentalized DNA replication and gene expression. New experimental perspectives are discussed. [less ▲]

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See detailEntre science et humanisme : un criminologue dans les murs
De Coninck, Gérard; Seron, Vincent ULg

in Born, Michel; Kefer, Fabienne; Lemaître, André (Eds.) Une criminologie de la tradition à l’innovation. En hommage à Georges Kellens (2006)

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See detailRecombinant bovine herpesvirus 4 (BoHV-4) expressing glycoprotein D of BoHV-1 is immunogenic and elicits serum-neutralizing antibodies against BoHV-1 in a rabbit model.
Donofrio, Gaetano; Cavirani, Sandro; Vanderplasschen, Alain ULg et al

in Clinical and Vaccine Immunology (2006), 13(11), 1246-54

Several biological characteristics of bovine herpesvirus 4 (BoHV-4) make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity ... [more ▼]

Several biological characteristics of bovine herpesvirus 4 (BoHV-4) make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types coming from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. Starting from BoHV-4 cloned as a bacterial artificial chromosome (BAC), we used MuA transposase-mediated in vitro transposition to generate recombinant BoHV-4 expressing the immunodominant glycoprotein D (gD) of BoHV-1, one of the most important pathogens of cattle. Although a cis-acting element from woodchuck hepatitis virus (the woodchuck hepatitis virus posttranscriptional regulatory element [WPRE]) in the 3' end of the gD expression cassette was required for maximal gD expression from plasmids in transient transfection assays, this element was not necessary for efficient expression of gD from recombinant BoHV-4 genomes. BoHV-4 recombinants containing gD expression cassettes with or without the WPRE expressed gD at similarly high levels. Several cell lines originating from different animal species expressed gD when infected with BoHV-4 recombinants. When rabbits were immunized with one of the recombinants, high levels of serum neutralizing antibodies against BoHV-1 were generated. This work is one of the first demonstrations of the use BoHV-4 as a vector for vaccine purposes and may provide the basis for BoHV-1 vaccination of cattle with recombinant BoHV-4. [less ▲]

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See detailValidation of a potentiometric method using azithromycin selective electrode
Rachidi, Mohamed ULg; Cherra, Y.; Digua, K. et al

Poster (2006)

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See detailPotential Estimation of Fatty Acid Content in Cow Milk by Mid-Infrared Spectrometry
Soyeurt, Hélène ULg; Dardenne, Pierre; Lognay, Georges ULg et al

in Prooceedings of the 35th ICAR session (2006)

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See detailEtude de la diversité des pucerons et des auxiliaires adiphages relative à la présence d'orties en bordures de champ
Alhmedi, A.; Francis, Frédéric ULg; Bodson, Bernard ULg et al

in Notes Fauniques de Gembloux (2006), 59(2), 121-124

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See detailHistological study of the horse stifle menisci in relation with ultrasonographic aspect: preliminary study
De Busscher, Virginie; Schreder, Anelaure; Busoni, Valeria ULg et al

in Italian Journal of Anatomy and Embryology (2006), 111

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See detailA structural, infrared, and Mossbauer spectral study of rosemaryite, NaMnFe3+Al(PO4)(3)
Hatert, Frédéric ULg; Hermann, R. P.; Fransolet, André-Mathieu ULg et al

in European Journal of Mineralogy (2006), 18(6, NOV-DEC), 775-785

Rosemaryite, ideally NaMnFe(3+)AI(PO4)(3), has been collected in the Buranga pegmatite, Rwanda. A single-crystal structure refinement was performed to R-1 = 4.01 %, in the P2(1)/n space group, with a = 12 ... [more ▼]

Rosemaryite, ideally NaMnFe(3+)AI(PO4)(3), has been collected in the Buranga pegmatite, Rwanda. A single-crystal structure refinement was performed to R-1 = 4.01 %, in the P2(1)/n space group, with a = 12.001(2), b = 12.396(1), c = 6.329(1) angstrom, beta 114.48(1)degrees, Vol. = 856.9(2) angstrom(3), Z = 4. The crystal structure and cation distributions are similar to those of ferrorosemaryite, NaFe2+Fe3+Al(PO4)(3), and qingheiite, Na2MnMgAl(PO4)(3), but aluminium predominantly occurs in the M(2a) site, not in the M(2b) site as observed in ferrowyllieite, Na2Fe22+Al(PO4)(3). The topologies of the X(1a) and X(1b) crystallographic sites are identical to those found in ferrorosemaryite, and correspond to a distorted octahedron and to a distorted cube, respectively. The [7+1]-coordinated X(2) site is a very distorted gable disphenoid, similar to the A(2)' site of the alluaudite structure. Mossbauer spectra have been obtained from 4.2 to 295 K, and fitted with a model including two Fe3+ and two Fe2+ doublets. The Fe2+ component corresponding to 2/3 of the Fe2+ spectral area and having a smaller quadrupole splitting of 2.63 mm/s at 15 K, is assigned to the Fe2+ on the M(2a) site, and the Fe2+ component with the larger quadrupole splitting of 3.17 mm/s at 15 K, is assigned to the Fe2+ on the M(1) site. Fe3+ is located only at the M(2a) and M(2b) sites, and the Fe3+ component corresponding to 3/4 of the Fe3+ and exhibiting the larger quadrupole spitting of 0.77 mm/s at 15 K, is most likely associated with Fe3+ on the M(2b) site. The infrared spectrum of rosemaryite shows absorption bands at 3450 and 1624 cm(-1), bands that arise from the vibrational modes of H2O and confirm the presence of water in the channels of the wyllieite structure. A comparison of both the Mossbauer spectra and structural data of rosemaryite with those of other phosphates of the alluaudite and wyllieite groups, is also presented. [less ▲]

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See detailAgriculture in Belgium : main characteristics and problems
Burny, Philippe ULg

in Lorencowicz, Edmund (Ed.) International Scientific Seminar "Farm machinery management in sustainable agriculture" : Seminar Proceedings (2006)

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See detailSome comments on the Parametric Fire Model of Eurocode 1
Reitgrüber, Stefan; Pérez-Jimenez, Christian; Di Blasi, Colomba et al

Conference (2006)

In this paper, the modifications that have been recently introduced in the parametric fire model of Eurocode 1 are presented. The reasons behind these modifications are given. Some Problems that have been ... [more ▼]

In this paper, the modifications that have been recently introduced in the parametric fire model of Eurocode 1 are presented. The reasons behind these modifications are given. Some Problems that have been discovered in the present formulation are highlighted, namely the fact that the model is not continuous and the fact that the heat release of wood that has been used for the calibration of the model is not consistent anymore with the value that is now recommended in the Eurocode. A proposal is made that makes the model continuous. A new calibration of this modified model has been made on the base of the now recommended value of the heat release of wood with comparison to the results of some 50 full scale experimental fire tests. [less ▲]

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See detailSelection of bacteria to decrease in vitro growth of Campylobacter jejuni and Campylobacter coli and characterization of their antagonistic activities
Marcq, Christopher ULg; Didderen, Isabelle; Dubois Dauphin, Robin ULg et al

in Proceedings of the Eleventh Conference on Food Microbiology (2006)

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See detailLa libre circulation des personnes dans l'Union européenne (1er janvier-31 décembre 2005)
Carlier, Jean-Yves ULg

in Journal de Droit Européen [=JDE] (2006), 14(127), 75-82

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See detailLe cycle des femmes vertueuses provenant de Herkenrode et sa restauration. Histoire et iconographie du cycle
Oger, Cécile ULg

in Denhaene, Godelieve (Ed.) Lambert Lombard, Peintre de la Renaissance. Liège 1505/06-1566 :Essais interdiscipinaires et catalogue de l'exposition (2006)

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See detailSimulation of thixoforming: comparison of different constitutive equations
Pierret, Jean-Christophe ULg; Rassili, Ahmed ULg; Cezard, Pierre

in Solid State Phenomena (2006), 116-117

The behaviour of the semi-solid material is complex to simulate and many approaches are proposed in the literature to represent it. In this work, different constitutive equations are studied: the ... [more ▼]

The behaviour of the semi-solid material is complex to simulate and many approaches are proposed in the literature to represent it. In this work, different constitutive equations are studied: the classical Northon-Hoff law, Joly and Merhabian's one [1] and an innovative micromechanical model using homogenisation technique [2]. These constitutive equations have been implemented in the FE code Forge2 (R) to simulate simple forming tests, such as compression and direct extrusion. The load-displacement curves using the three constitutive equations are compared in order to estimate their capabilities. [less ▲]

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See detailEtat de la question sur l'hyperactivité et les troubles attentionnels
Catale, Corinne ULg

Conference given outside the academic context (2006)

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See detailL'émission trading à la Cour d'arbitrage, Commentaire de C.A., 92/2006
Pâques, Michel ULg

in Aménagement - Environnement (2006), 4

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See detailRobust analysis of silhouettes by morphological size distributions
Barnich, Olivier ULg; JODOGNE, Sébastien ULg; Van Droogenbroeck, Marc ULg

in Lecture Notes in Computer Science (2006), 4179

We address the topic of real-time analysis and recognition of silhouettes. The method that we propose first produces object features obtained by a new type of morphological operators, which can be seen as ... [more ▼]

We address the topic of real-time analysis and recognition of silhouettes. The method that we propose first produces object features obtained by a new type of morphological operators, which can be seen as an extension of existing granulometric filters, and then insert them into a tailored classification scheme. Intuitively, given a binary segmented image, our operator produces the set of all the largest rectangles that can be wedged inside any connected component of the image. The latter are obtained by a standard background subtraction technique and morphological filtering. To classify connected components into one of the known object categories, the rectangles of a connected component are submitted to a machine learning algorithm called EXtremely RAndomized trees (Extra-trees). The machine learning algorithm is fed with a static database of silhouettes that contains both positive and negative instances. The whole process, including image processing and rectangle classification, is carried out in real-time. Finally we evaluate our approach on one of today's hot topics: the detection of human silhouettes. We discuss experimental results and show that our method is stable and computationally effective. Therefore, we assess that algorithms like ours introduce new ways for the detection of humans in video sequences. [less ▲]

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