A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep.
; Marcq, Fabienne ; Takeda, Haruko et al
in Nature Genetics (2006), 38(7), 813-8
Texel sheep are renowned for their exceptional meatiness. To identify the genes underlying this economically important feature, we performed a whole-genome scan in a Romanov x Texel F2 population. We ... [more ▼]
Texel sheep are renowned for their exceptional meatiness. To identify the genes underlying this economically important feature, we performed a whole-genome scan in a Romanov x Texel F2 population. We mapped a quantitative trait locus with a major effect on muscle mass to chromosome 2 and subsequently fine-mapped it to a chromosome interval encompassing the myostatin (GDF8) gene. We herein demonstrate that the GDF8 allele of Texel sheep is characterized by a G to A transition in the 3' UTR that creates a target site for mir1 and mir206, microRNAs (miRNAs) that are highly expressed in skeletal muscle. This causes translational inhibition of the myostatin gene and hence contributes to the muscular hypertrophy of Texel sheep. Analysis of SNP databases for humans and mice demonstrates that mutations creating or destroying putative miRNA target sites are abundant and might be important effectors of phenotypic variation. [less ▲]Detailed reference viewed: 65 (7 ULg)
Diagnosis of osteoporosis without prevalent fractures: are we missing our main target?
; Reginster, Jean-Yves
in Osteoporosis Action (2006), 1Detailed reference viewed: 9 (3 ULg)
De Mickey Mouse à Médiamarkt : gouvernance de la sécurité
Diverse speeche and writing (2006)Detailed reference viewed: 26 (10 ULg)
Georges Didi-Huberman: vers une intentionnalité inversée ?
in La part de l'oeil (2006), 21-22Detailed reference viewed: 57 (0 ULg)
Effets symptomatiques de la glucosamine dans l’arthrose
Reginster, Jean-Yves ; Bruyère, Olivier
in Pharma-Sphère (2006), 110Detailed reference viewed: 21 (10 ULg)
Les interventions auprès des groupes et des équipes en situation de souffrance relationnelle au travail : réflexions générales et méthodologies particulières
Report (2006)Detailed reference viewed: 34 (9 ULg)
Analytical validation of potentiometric method for cetirizinium ion
Rachidi, Mohamed ; ; Hubert, Philippe et al
in Analytical Letters (2006), 39(8), 1699-1708
Accuracy and reliability of the analytical results are crucial for ensuring quality, safety, and efficacy of pharmaceutical products. However, to ensure these criteria, analytical validation is required ... [more ▼]
Accuracy and reliability of the analytical results are crucial for ensuring quality, safety, and efficacy of pharmaceutical products. However, to ensure these criteria, analytical validation is required. In this matter, many official documents describing the criteria of validation are available. However, these concern mainly chromatography analysis and bio-analytical methods of pharmaceutical products, but they do not propose any experimental protocol for direct potentiometric methods using ion-selective electrodes applied in pharmaceutical analysis. In this work, we are proposing a validation strategy based on the normative and regulatory guidelines applied to a potentiometric method with a polymeric membrane selective to cetirizine dihydrochloride. The statistical analysis obtained from raw data is described for all steps of this protocol. Also this validated method was successfully applied to the determination of cetirizine in pharmaceutical preparations using direct potentiometry with a mean relative standard deviation of 0.57% and a mean recovery of 99.63%. [less ▲]Detailed reference viewed: 226 (5 ULg)
Adherence to anti-osteoporotic treatment: does it really matter?
Reginster, Jean-Yves ; Rabenda, Véronique
in Future Rheumatology (2006), 1Detailed reference viewed: 7 (3 ULg)
La consommation de lait et produits laitiers en Belgique et en Région wallonne
Duquesne, Brigitte ; ; Lebailly, Philippe
Poster (2006)Detailed reference viewed: 158 (10 ULg)
L'usage exclusif d'un vaccin inactivé pour la prévention médicale de la maladie d'Aujeszky du chien de chasse
Muylkens, Benoît ; Linden, Annick ; Thiry, Etienne
Article for general public (2006)Detailed reference viewed: 27 (6 ULg)
Langues et immigration dans l’enseignement en Communauté française de Belgique
in Conseil supérieur de la langue française et Service de la langue française de la Communauté française de Belgique (Ed.) Langue française et diversité linguistique (2006)Detailed reference viewed: 54 (4 ULg)
The parental experience and perception of giving birth under water for their first child and the related first parent-child interactions.
Naziri, Despina ; ;
Poster (2006)Detailed reference viewed: 7 (2 ULg)
Proteomic analysis of ovine muscle hypertrophy.
; ; et al
in Journal of Animal Science (2006), 84(12), 3266-76
Two-dimensional electrophoresis was used to investigate the effects of a QTL for muscle hypertrophy on sarcoplasmic protein expression in ovine muscles. In the Belgian Texel breed, the QTL for muscle ... [more ▼]
Two-dimensional electrophoresis was used to investigate the effects of a QTL for muscle hypertrophy on sarcoplasmic protein expression in ovine muscles. In the Belgian Texel breed, the QTL for muscle hypertrophy is localized in the myostatin-encoding gene. Based on microsatellite markers flanking the myostatin gene, we compared the hypertrophied genotype with the normal genotype. The average age of the sheep was 3 mo. Among the 4 muscles studied, in the hypertrophied genotype only the vastus medialis was normal, whereas the semimembranosus, tensor fasciae latae, and LM were hypertrophied. In the hypertrophied genotype, these muscles showed upregulation of enzymes involved in glycolytic metabolism together with oxidative metabolism in LM. Certain chaperone proteins, including glutathione S-transferase-Pi, heat shock protein-27, and heat shock cognate-70, were also more highly expressed, probably due to increased use of energetic pathways. Expression of the iron transport protein transferrin was increased. Alpha-1-antitrypsin was the only protein showing a similar pattern of expression (i.e., less expressed) in all 4 muscles of the hypertrophied genotype. It is suggested that transferrin and alpha-1-antitrypsin may interact to reinforce myogenic proliferative signaling. [less ▲]Detailed reference viewed: 18 (1 ULg)
Preliminary development of real time PCR for quantification of Erwinia species infecting potato tubers.
Massart, Sébastien ; ; et al
in Communications in agricultural and applied biological sciences (2006), 71(3 Pt B), 1171-1173Detailed reference viewed: 22 (1 ULg)
Inclusion of a stress-dependent Preisach model in 2D FE calculations
; ; et al
in Compel-the International Journal for Computation and Mathematics in Electrical and Electronic Engineering (2006), 25(1), 81-90
Purpose - Mechanical stress can heavily affect the magnetic behaviour law in ferromagnetic materials. This paper, aims to take into account the effect of mechanical stress into a hystreresis model. This ... [more ▼]
Purpose - Mechanical stress can heavily affect the magnetic behaviour law in ferromagnetic materials. This paper, aims to take into account the effect of mechanical stress into a hystreresis model. This model is implemented in a finite element analysis code and tested in the case of a simple system. Design/methodology/approach - A simple extension of the classical Preisach model is proposed, in which a function linked to the Preisach density is parameterized using the mechanical stress as a supplementary parameter. The methodology is based on experimental measurements for identifying the required function. As a first approach, a linear interpolation is used between the measurements in order to have a continuous evolution of the magneto-mechanical behaviour. This model has been tested in the case of a steel sheet in which width is not constant in order to obtain a non-uniform distribution of stress and magnetic flux density. Findings - The model can predict the magneto-mechanical behaviour with a good accuracy in the case of tensile stress. Implementation of the model in finite element analysis has shown that the model can predict the behaviour of steel sheet subject to a non-uniform stress distribution. Originality/value - This paper shows that a classical hysteresis model can be extended to take into account the magneto-mechanical behaviour. This is useful for the design of electrical machines which are subject to non-negligible mechanical stress. [less ▲]Detailed reference viewed: 18 (0 ULg)
Mögliches und Unmögliches zwischen Dialekt und Standard. Kookkurrenzrestriktionen als Zugang zur Struktur regionaler Umgangssprache im Rheinland
in Osnabrücker Beiträge zur Sprachtheorie (2006), 71Detailed reference viewed: 41 (3 ULg)
Etude en temps réel du processus d'extraction de la tagitinine C en fonction des caractéristiques physico-chimiques du CO2 supercritique à l'aide de fibres optiques couplant un spectrophotomètre IRTF à un extracteur à fluide supercritique
Ziemons, Eric ; Lejeune, Robert ; Angenot, Luc et al
Poster (2006)Detailed reference viewed: 29 (3 ULg)