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See detail"Dies verhüllte Genießen der Musik ohne Töne" : Robert Schumanns Reflexionen über das Medium Schrift.
Viehöver, Vera ULg

in Herwig, Henriette; Kalisch, Volker; Kortländer, Bernd (Eds.) et al ^Übergänge : Zwischen Künsten und Kulturen : Akten des Kongresses zum 150. Todestag von Heinrich Heine und Robert Schumann (2006)

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See detailStudy of a bisquaternary ammonium salt by atmospheric pressure photoionization mass spectrometry
Giuliani, Alexandre; Debois, Delphine ULg; Laprévote, Olivier

in European Journal of Mass Spectrometry (Chichester, England) (2006), 12(3), 189-197

A comprehensive atmospheric pressure photoionization (APPI) mass spectrometry investigation of hexamethonium bromide is reported. This bisquaternary ammonium salt is a model system for the investigation ... [more ▼]

A comprehensive atmospheric pressure photoionization (APPI) mass spectrometry investigation of hexamethonium bromide is reported. This bisquaternary ammonium salt is a model system for the investigation of multiply charged species and elucidation of ion formation processes. It has been used to elucidate the physico-chemical phenomenon occurring when photoionization is carried out at atmospheric pressure. First, the in-source fragmentations were studied for aqueous solutions of the salt with the photoionization lamp switched off, i.e. under thermospray conditions. It is shown that, in this mode of operation, fragmentations are minor and may be classified into two classes, namely dequaternization and charge separation, arising from the two precursors, M2+ and [M+Br]+. Second, the fragmentation patterns have been monitored in dopant-assisted APPI for different dopants (toluene, toluene-d8 anisole and hexafluorobenzene) at various amounts. At low dopant flow rates, the [M+Br]+ and M2+ ions are still observed. As the flow rate is increased, these precursor ions lose intensity and are finally suppressed for all three dopants. Comparison of toluene and toluene-d8 reveals that H atoms may be transferred from the dopant to the molecular ions, very likely mediated by the solvent. The role of the solvent (water) was also investigated by using heavy water. Apart from the thermospray fragmentations, which are also observed in APPI, several fragmentation pathways appear to be specific to the photoionization process. Photoionization efficiencies are measured by determination of the relative photoionization cross sections with respect to toluene. It is found that, when the ionization efficiencies are taken into account, the depletion of the precursors as a function of the dopant flow rates is the same for all three dopant molecules. This result shows that the precursor ions are depleted by reactions with the photoelectrons released from the dopant. Three additional mechanisms are proposed to account for this effect: electron transfer or H atom transfer from negatively charged water nanodroplets and H atom transfer from the dopant. [less ▲]

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See detailQuantitative risk assessment of Campylobacter spp. in poultry based meat preparations as one of the factors to support the development of risk-based microbiological criteria in Belgium
Uyttendaele, Mieke; Baert, Katleen; Ghafir, Yasmine et al

in International Journal of Food Microbiology (2006), 111(2), 149-163

The objective of this study was to do an exercise in risk assessment on Campylobaeter spp. for poultry based meat preparations in Belgium. This risk assessment was undertaken on the demand of the ... [more ▼]

The objective of this study was to do an exercise in risk assessment on Campylobaeter spp. for poultry based meat preparations in Belgium. This risk assessment was undertaken on the demand of the competent national authorities as one of the supportive factors to define fisk-based microbiological criteria. The quantitative risk assessment model follows a retail to table approach and is divided in different modules. The contamination of raw chicken meat products (CMPs) was represented by a normal distribution of the natural logarithm of the concentration of Campylobacter spp. (In[Camp]) in raw CMPs based on data from surveillance programs in Belgium. To analyse the relative impact of reducing the risk of campylobacteriosis associated with a decrease in the Campylobacter contamination level in these types of food products, the model was run for different means and standard deviations of the normal distribution of the ln[Camp] in raw CMPs. The limitation in data for the local situation in Belgium and on this particular product and more precisely the semi-quantitative nature of concentration of Campylobacter spp. due to presence/absence testing, was identified as an important information gap. Also the knowledge on the dose-response relationship of Campylobacter spp. was limited, and therefore three different approaches of dose-response modelling were compared. Two approaches (1 and 2), derived from the same study, showed that the reduction of the mean of the distribution representing the ln[Camp] in raw CMPs is the best approach to reduce the risk of Campylobacter spp. in CMPs. However, for the simulated exposure and approach 3 it was observed that the reduction of the standard deviation is the most appropriate technique to lower the risk of campylobacteriosis. Since the dose-response models used in approach I and 2 are based on limited data and the reduction of the mean corresponds with a complete shift of the contamination level of raw CMPs, demanding high efforts from the poultry industry, it is proposed to lower the standard deviation of the concentration of Campylobacter spp. in raw CMPs. This proposal corresponds with the elimination of the products that are highly contaminated. Simulation showed that eating raw chicken meat products can give rise to exposures that are 10(10) times higher than when the product is heated, indicating that campaigns are important to inform consumers about the necessity of an appropriate heat treatment of these type of food products. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailQuantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE).
Asquith, Becca; Debacq, Christophe; Florins, Arnaud-Francois ULg et al

in Proceedings of the Royal Society B : Biological Sciences (2006), 273(1590), 1165-71

The cytoplasmic dye carboxyfluorescein diacetate succinimidyl ester (CFSE) is used to quantify cell kinetics. It is particularly important in studies of lymphocyte homeostasis where its labelling of cells ... [more ▼]

The cytoplasmic dye carboxyfluorescein diacetate succinimidyl ester (CFSE) is used to quantify cell kinetics. It is particularly important in studies of lymphocyte homeostasis where its labelling of cells irrespective of their stage in the cell cycle makes it preferable to deuterated glucose and BrdU, which only label dividing cells and thus produce unrepresentative results. In the past, experiments have been limited by the need to obtain a clear separation of CFSE peaks forcing scientists to adopt a strategy of in vitro labelling of cells followed by their injection into the host. Here we develop a framework for analysis of in vivo CFSE labelling data. This enables us to estimate the rate of proliferation and death of lymphocytes in situ, and thus represents a considerable advance over current procedures. We illustrate this approach using in vivo CFSE labelling of B lymphocytes in sheep. [less ▲]

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See detailWind tunnel protocol for spray drift assessment
Lebeau, F.; Stainier, C.; Destain, M.-F. et al

in Parasitica (2006), 62(1-2), 22

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See detailWhat did we recently learned on and from equine Doppler echocardiography ?
Amory, Hélène ULg; Pirard, L; Sandersen, Charlotte ULg

in Proceedings of the Resident Meeting of the European College of Equine Internal medicine (ECEIM) (2006)

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See detailHigh-Resolution GC Coupled to High-Resolution MS in the Analysis of Dioxins and Related Substances, Principles and Applications
Eppe, Gauthier ULg; De Pauw, Edwin ULg; Focant, Jean-François ULg

in Niessen, W. M. A. (Ed.) The Encyclopedia of Mass Spectrometry, Volume 8, Hyphenated Methods (2006)

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See detailDescriptive and spatial epidemiology of Rift valley fever outbreak in Yemen 2000-2001
Abdo-Salem, S.; Gerbier, G.; Bonnet, Pascal et al

in Annals of the New York Academy of Sciences (2006), 1081

Rift valley fever (RVF) is an arboviral disease produced by a bunyavirus belonging to the genus Phlebovirus. Several species of Aedes and Culex are the vectors of this virus that affects sheep, goats ... [more ▼]

Rift valley fever (RVF) is an arboviral disease produced by a bunyavirus belonging to the genus Phlebovirus. Several species of Aedes and Culex are the vectors of this virus that affects sheep, goats, buffalos, cattle, camels and human beings. The human disease is well known, especially during periods of intense epizootic activity. The initial description of the disease dates back to 1930, when animals and human outbreaks appeared on a farm in Lake Naivasha, in the Great Rift Valley of Kenya. Until 2000, this disease was only described in Africa, and then outbreaks were also declared in the Kingdom of Saudi Arabia (2000-2001 and 2004) and in Yemen (2000-2001). Animal and human cases were recorded. This work presents a retrospective summary of the data collected on animal RVF cases during this epidemic in Yemen. Results from several RVF surveys were gathered from the Yemeni vet services and FAO experts. Geographical data (topographic maps and data freely available on internet) were used for the location of outbreaks. After cleaning and standardization of location names, all the data were introduced into a GIS database. The spatial distribution of outbreaks was then studied at two scales: at the national level and at a local scale in the particular area of Wadi Mawr in the Tihama plain, Western coast of Yemen. [less ▲]

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See detailImpact of the invasive alien plant Solidago giganteaon primary productivity, plant nutrient content and soil mineral nutrient concentrations
Vanderhoeven, SONIA ULg; Dassonville, Nicolas; Chapuis-Lardy, Lydie et al

in Plant and Soil (2006), 286(1-2), 259-268

Invasion by alien plants can alter ecosystem processes and soil properties. In this study, we compared aboveground productivity, nutrient pools in standing biomass and topsoil (0-0.10 m) mineral nutrient ... [more ▼]

Invasion by alien plants can alter ecosystem processes and soil properties. In this study, we compared aboveground productivity, nutrient pools in standing biomass and topsoil (0-0.10 m) mineral nutrient concentrations between plots invaded by Early Goldenrod (Solidago gigantea) and adjacent, uninvaded, vegetation at five sites in Belgium. The five sites were characterised by a resident perennial herbaceous vegetation and spanned a wide range in soil fertility level and floristic composition. Invaded stands consistently had higher (2-3-fold) aboveground productivity and lower mineral element concentrations in standing phytomass. Nutrient pools (calculated as concentration x phytomass) was ca. twice higher in invaded plots, suggesting that S. gigantea might enhance nutrient cycling rates. Impacts on topsoil chemistry were surprisingly modest, with slightly higher nutrient concentrations under the invader. A noticeable exception was phosphorus, which showed higher concentrations of ammonium acetate-extractable fraction in invaded plots in four of five sites. It appears that S. gigantea does not significantly contribute to nutrient uplift from deep soil layers to topsoil, possibly because it does not root much deeper compared to resident vegetation. [less ▲]

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See detailExperimental evidences of a structural and dynamical transition in fish school
Becco, Christophe ULg; Vandewalle, Nicolas ULg; Delcourt, Johann ULg et al

in Physica A: Statistical Mechanics and its Applications (2006), 367

We have developed a video tracking system in order to determine all the trajectories of young fish (Oreochromis niloticus L.) within a school. Both individual and collective behaviours have been studied ... [more ▼]

We have developed a video tracking system in order to determine all the trajectories of young fish (Oreochromis niloticus L.) within a school. Both individual and collective behaviours have been studied as a function of the number of fish per unit area. By studying distributions of distances between fish and distributions of relative orientations, structural effects and cooperative motions have been evidenced. Signatures of a phase transition have been found, as predicted by some numerical models. This work opens new perspectives in the study of collective phenomena in biological systems since it is the first time that such measurements are possible. [less ▲]

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See detailL'érosion de la biodiversité: les carabides. Etat de l'Environnement wallon
Dufrêne, Marc ULg; Desender, Konjev

Report (2006)

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See detailDynamics of spontaneous ventricular fibrillation in acutely ischemic pigs
Desaive, Thomas ULg; Janssen, Nathalie ULg; Péters, F. et al

in Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology (2006), 8 (supplement 1)

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See detailNumerical study of the fatigue crack in welded beam-to-column connection using cohesive zone model
Lequesne, Cédric ULg; Plumier, André ULg; Degée, Hervé ULg et al

in Proceedings of the International Conference on Fracture and Damage Mechanics V (2006)

The fatigue behaviour of the welded beam-to-column connections of steel moment resisting frame in seismic area must be evaluated. The cohesive zone model is an efficient solution to study such connections ... [more ▼]

The fatigue behaviour of the welded beam-to-column connections of steel moment resisting frame in seismic area must be evaluated. The cohesive zone model is an efficient solution to study such connections by finite elements. It respects the energetic conservation and avoids numerical issues. A three-dimensional cohesive zone model element has been implemented in the home made finite element code Lagamine [1]. It is coupled with the fatigue continuum damage model of Lemaître and Chaboche [2]. The cohesive parameters are identified by the inverse method applied on a three points bending test modelling. [less ▲]

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See detailImpact of acute cadmium exposure on the trunk lateral line neuromasts and consequences on the "C-start" response behaviour of the sea bass (Dicentrarchus labrax L.; Teleostei, Moronidae).
Faucher, Karine ULg; Fichet, Denis; Miramand, Pierre et al

in Aquatic Toxicology (2006), 76(3-4), 278-94

Behavioural responses of sea bass Dicentrarchus labrax were investigated after exposure to cadmium ions in laboratory-controlled conditions. The aim of this study was to discover whether environmental ... [more ▼]

Behavioural responses of sea bass Dicentrarchus labrax were investigated after exposure to cadmium ions in laboratory-controlled conditions. The aim of this study was to discover whether environmental exposure to cadmium ions inactivates fish lateral line system neuromasts, and to determine the behavioural consequences of such a sensory blockage. For this, fish escape behaviour in response to an artificial water jet was recorded using a 25-frames s(-1) analog video camera before and after cadmium exposure. Experimental set up was tested with fish whose lateral line system was artificially inactivated by antibiotics (gentamicin and streptomycin). Histological analyses with scanning electron microscopy showed antibiotic treatment destroyed lateral line system neuromasts. In addition, these fish did not respond to stimulations provoked by the water jet after antibiotic treatment. Fish escape behaviour was then recorded before and after cadmium exposure at two different concentrations. When fish were exposed to the first concentration of cadmium tested (0.5 microg l(-1), which represents the maximal cadmium concentration encountered in contaminated estuaries), no alteration in neuromast tissue was observed. In addition, before cadmium exposure, fish responded positively in 98.41 +/- 4.95% of lateral line system stimulations (escape behaviour in response to the water jet). After cadmium exposure, no behavioural modification could be detected: the fish responded positively in 95.16 +/- 9.79% of stimulations (chi(2) = 2.464, p = 0.116). In contrast, the high cadmium concentration used (5 microg l(-1), which represents 10 times the concentration occurring in highly polluted estuarine areas) involved severe neuromast tissue damage. Just after such cadmium exposure, fish showed only 41.67 +/- 35.36% of positive responses to their lateral line system stimulations, while they responded positively in 95.93 +/- 9.10% of stimulations under control conditions (chi(2) = 24.562, p < 0.0001). Their lateral line system neuromasts seemed to regenerate about 1 month after cadmium exposure. Associated with this regeneration, from the 21st day after cadmium exposure, their escape behaviour had recovered and was not significantly different from that recorded under control conditions (86.74 +/- 20.82%, chi(2) = 2.876, p = 0.090). This study shows that although 5 microg l(-1) cadmium is able to damage lateral line system neuromasts and causes fish behavioural alterations, fish exposed to 0.5 microg l(-1) cadmium displayed neither tissue neuromast nor behavioural modification. [less ▲]

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