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See detailReal-time RT-PCR quantification of mRNA encoding cytokines, CC chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneurnopathy
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Immunology and Immunopathology (2006), 110(1-2), 65-77

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been ... [more ▼]

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been discovered. A recent study, examining the relative proportion of various lymphocyte cell subsets within bronchoalveolar lavage fluid from dogs with EBP, has shown a selective increase in CD4(+) T-cells and a selective decrease in CD8(+) T-cells, suggesting that a similar Th2 immune response might occur in EBP. The aim of the present study was to determine the profile of cytokine, chemokine and CC chemokine receptor 3 (CCR3) messenger RNA (mRNA) expression in bronchial tissue from dogs with EBP. Real-time RT-PCR assays were used for the quantification of mRNA encoding for a panel of cytokines, CC chemokines and CCR3 in perendoscopic bronchial biopsies from eight dogs with EBP and seven age-matched control dogs. Messenger RNA transcribed from the housekeeping gene glyceraldehyde-3 -phosphate dehydrogenase was used for normalisation of the threshold cycle in order to determine the relative copy numbers of the transcripts. No significant difference in the expression of any cytokine, MCP-1, -2, -4 and CCR3 was found between control and EBP dogs. The expression of transcript for MCP-3, eotaxin-2 and -3 was significantly greater in bronchial biopsies from dogs with EBP than in samples from control dogs while there was significantly less mRNA encoding RANTES in the mucosa of dogs with EBP. In conclusion, the cytokine mRNA expression profile in perendoscopic bronchial biopsies is similar in dogs with EBP and dogs without respiratory disease. Further studies on the quantification of mRNA encoding cytokines in isolated T lymphocytes from bronchoalveolar lavage fluid or bronchial biopsies are needed before any conclusion on the cytokine profile in canine EBP can be drawn. Eotaxin-2, -3 and MCP-3 appear to be implicated in the pathogenesis of the disease. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailLe nez électronique, un nouvel outil diagnostique pour les maladies respiratoires du cheval ?
Salinas, Emmanuelle; Ramery, Eve ULg; Fraipont, Audrey ULg et al

in Proceedings: AVEF, Versailles, France (2006)

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See detailL'évaluation des projets de prévention des assuétudes
Absil, Gaëtan ULg; Vandoorne, Chantal ULg; Coupienne, Valérie et al

in Observatoire : Revue d'Action Sociale & Médico-Sociale (2006), (51-52), 139-145

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See detailExercise and pharmacological echocardiography stress in healthy horses
Sandersen, Charlotte ULg; Detilleux, Johann ULg; Art, Tatiana ULg et al

in Proceedings of the 7th ICEEP (Equine veterinary supplement 206, 36:159-162) (2006)

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See detailDegré d'atteinte nerveuse périphérique dans la SLA, la SLP et la maladie de Kennedy
WANG, François-Charles ULg; Le Forestier, Nadine; GERARD, Pascale ULg et al

in Neurophysiologie Clinique = Clinical Neurophysiology (2006)

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See detailRelationship between haptoglobin, serum amyloid A, and clinical status in a survey of dairy herds during a 6-month period
Humblet, Marie-France ULg; Guyot, Hugues ULg; Boudry, Benjamin et al

in Veterinary Clinical Pathology (2006), 35(2), 188-193

Haptoglobin and serum amyloid A are major acute phase proteins in cattle. Dairy cattle often develop pathologic conditions in the peripartum period; acute phase proteins may be useful in their diagnosis ... [more ▼]

Haptoglobin and serum amyloid A are major acute phase proteins in cattle. Dairy cattle often develop pathologic conditions in the peripartum period; acute phase proteins may be useful in their diagnosis. Objectives: The purpose of this study was to compare the accuracy of serum haptoglobin (Hp) and serum amyloid A (SAA) concentrations with clinical health status for diagnosing disease during the peripartum period in dairy cattle. Methods: Dairy cows from 4 herds were evaluated every 15 days over a 6-month period. Health status was determined by thorough clinical examination. Haptoglobin and SAA concentrations were measured in serum using validated methods and the results were classifed as positive or negative based on defined cutoff points. Disease prevalence, sensitivity, and specificity were compared using clinical examination as the gold standard. Results: A total of 1896 samples from 158 cows were analyzed. Significant increases in mean Hp and SAA concentrations were observed in the week following parturition in both primiparous and multiparous cows, although high interindividual variability was observed. Both Hp and SAA had low sensitivity but higher specificity in determining disease status compared with clinical examination. Increased concentrations of Hp and SAAwere found in ,10% of samples from clinically healthy cows, except in the week after parturition. Conclusions: Haptoglobin and serum amyloid A should be used with caution as markers of inflammation in the week after calving. Poor sensitivity in other postpartum periods could be related to the higher incidence of chronic (vs acute) inflammation. Haptoglobin may be appropriate for routine screening, but further work needs to be done to assess its value as an indicator of herd health. [less ▲]

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See detailLa "compétence discursive" en néerlandais langue étrangère: prosodie et syntaxe
Rasier, Laurent ULg

Scientific conference (2006)

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See detailContribution à la mission CoRoT et à la recherche d'exoplanètes par la méthode des transits photométriques
Gillon, Michaël ULg

Doctoral thesis (2006)

In this thesis, we present our contributions to the CoRoT mission and to the search for exoplanets with the photometric transit method. We developped a spectroscopic analysis method, APASS, which allows ... [more ▼]

In this thesis, we present our contributions to the CoRoT mission and to the search for exoplanets with the photometric transit method. We developped a spectroscopic analysis method, APASS, which allows to obtain a precise determination of atmospheric physical parameters and abundances for stars with an unnegligeable rotational speed. We present APASS and the high-precision results we obtained for main target stars of the asteroseismological part of the CoRoT mission. Our results are in agreement with those obtained by other authors using different analysis methods, while beeing more precise. Furthermore, they show that the use of a spectroscopic analysis method based on equivalent widths measurements by gaussian or Voigt profiles fitting should be avoided for stars whose rotational velocity is significantly higher then the Sun’s, and that a method using synthetic spectra fitting for which the convolution is a free parameter should be prefered. Next, we investigate the potential of extrasolar planet transit surveys. We show that CoRoT has a very high potential for the detection of close-in giant planets, better than the one of the KEPLER mission. This latter reveals to be better for the detection of terrestrial planets. Nevertheless, CoRoT could detect a few giant telluric planets of short orbital period, provided that this kind of object is frequent in our galaxy. For ground-based surveys, our results based on the use of large telescopes and wide-field cameras seem to indicate that an important potential is also available from the ground. These results are nevertheless less reliable, mainly because of the lack of knowledge about the influence of systematic effects, and additional investigations using actual data will be necessary. We present then the deconvolution method MCS and the way we adapted it to planetary transit photometry. We present the results we obtained after analysis of actual data for OGLE-TR-123 (VLT/FORS2 data) and OGLE-TR-132 (VLT/FORS2 and NTT/SUSI2 data). These results are very encouraging and demonstrate the high potential of MCS for this kind of high-precision analysis. Finally, we present the future improvements we intend to install to obtain a still higher level of precision and to increase the speed of analysis. [less ▲]

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See detailManaging CSR in Complex Environments : Stakeholder Theory in Action
Xhauflair, Virginie ULg; Zune, Marc

in Allouche, José (Ed.) Corporate Social Responsibility : Performances and Stakeholders (2006)

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See detailImproving control of storage diseases on apple by combining biological and physical postharvest methods
Creemers, P.; Van Laer, S.; Vorstermans, B. et al

Poster (2006)

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See detailLe rôle pivot des variables latentes pourle raisonnement statistique conditionnel. Exemples d'applications environnementales des processus ponctuels marqués.
Parent, E.; Bernier, J.; Boreux, Jean-Jacques ULg

in Revue de Statistique Appliquée (2006), 54(4), 85-111

En statistique bayésienne, le raisonnement conditionnel probabiliste fonde la solidarité étroite entre modélisation et inférence. Dans le cadre de ce raisonnement, un rôle pivot est joué par les variables ... [more ▼]

En statistique bayésienne, le raisonnement conditionnel probabiliste fonde la solidarité étroite entre modélisation et inférence. Dans le cadre de ce raisonnement, un rôle pivot est joué par les variables latentes. Ce mode de raisonnement peut s’avérer utile aussi en statistique fréquentiste. On présente trois séries hydrométéorologiques décrites par des modèles de processus ponctuels marqués de complexité croissante. Sur ces exemples, on montre comment récolter les fruits de la souplesse de modélisation et des facilités de calcul apportées par les variables latentes. Ces variables améliorent la conceptualisation de variables de structure dans les modèles statistiques et permettent aux calculs d’inférence de bénéficier des algorithmes MCMC en relation avec les techniques «d’augmentation de données». [less ▲]

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See detailFinite element modelling of electrostatic MEMS including the impact of fringing field effects on forces
Boutaayamou, Mohamed ULg; Nair, Harish; V Sabariego, Ruth ULg et al

in Proceedings of the 7th International Conference on Thermal, Mechanical and Multiphysics Simulation and Experiments in Micro-Electronics and Micro-Systems (EuroSimE2006) (2006)

The numerical models describing the behaviour of electrostatically actuated microsystems often disregard fringing fields. However, taking the fringing fields into account is crucial for an accurate ... [more ▼]

The numerical models describing the behaviour of electrostatically actuated microsystems often disregard fringing fields. However, taking the fringing fields into account is crucial for an accurate computation of the electrostatic forces. In this work, the finite element method is applied for modeling electrostatic actuators. The electrostatic force distribution is obtained by locally applying the virtual work method. A micro-beam and a comb drive are considered as test cases. The impact of the fringing field effects on the accuracy of electrostatic forces is shown through 2D and 3D parametric studies. [less ▲]

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See detailCombined spastic paresis of both gastrocnemius and femoral quadriceps muscles in Belgian blue calves
Janda, Jozef; Uyttenhoef, Aude; Vandeputte, Sébastien ULg et al

Conference (2006)

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See detailTRAP-6, immobilized in microspheres, accelerates tissue regeneration in gastric ulcer rat model.
Rusanova, A.; Makarova, A.; Strukova, S. et al

in Bulletin of Experimental Biology and Medicine (2006), 142(7), 42-46

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See detailA cascade optimization methodology for automatic parameter identification and shape/process optimization in metal forming simulation
Ponthot, Jean-Philippe ULg; Kleinermann, Jean-Pascal

in Computer Methods in Applied Mechanics & Engineering (2006), 195(41-43), 5472-5508

Computer simulations of metal forming processes using the finite element method (FEM) are, today, well established. This form of simulation uses an increasing number of sophisticated geometrical and ... [more ▼]

Computer simulations of metal forming processes using the finite element method (FEM) are, today, well established. This form of simulation uses an increasing number of sophisticated geometrical and material models, relying on a certain number of input data, which are not always readily available. The aim of inverse problems, which will be considered here, is to determine one or more of the input data relating to these forming process simulations, thereby leading to a desired result. In this paper, we will focus on two categories of such inverse problems. The first category consists of parameter identification inverse problems. These involve evaluating the material parameters for material constitutive models that would lead to the most accurate results with respect to physical experiments, i.e. minimizing the difference between experimental results and FEM simulations. The second category consists of shape/process optimization inverse problems. These involve determining the initial geometry of the specimen and/or the shape of the forming tools, as well as some parameters of the process itself, in order to provide the desired final geometry after the forming process. These two categories of inverse problems can be formulated as optimization problems in a similar way, i.e. by using identical optimization algorithms. In this paper, we intend firstly to solve these two types of optimization problems by using different non-linear gradient based optimization methods and secondly to compare their efficiency and robustness in a variety of numerical applications. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailOn the definition and use of landscape metrics to disentangle landscape complexity.
Bogaert, Jan ULg

in Gibelli, G.; Brancucci, G. (Eds.) Pianificare l’Incertezza. L’Ecologia del Paesaggio nella Gestione dei Sistemi Territoriali. (2006)

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See detailQuantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.
Delroisse, Jean-Marc; Boulvin, Anne-Lise; Parmentier, Isabelle et al

in Microbiological research (2006), (5),

Detailed reference viewed: 69 (4 ULg)