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See detailHistone deacetylase HDAC8 associates with smooth muscle alpha-actin and is essential for smooth muscle cell contractility
Waltregny, David ULg; Glenisson, Wendy; Tran, Syv Li et al

in FASEB Journal (2005), 19(8), 966-968

Although originally characterized as nuclear enzymes controlling the stability of nucleosomes, histone deacetylases (HDACs) may also exert their activity within the cytosol. Recently, we have demonstrated ... [more ▼]

Although originally characterized as nuclear enzymes controlling the stability of nucleosomes, histone deacetylases (HDACs) may also exert their activity within the cytosol. Recently, we have demonstrated that HDAC8, a class I HDAC, is a novel, prominently cytosolic marker of smooth muscle differentiation. As HDAC8 displays a striking stress fiber-like pattern of distribution and is coexpressed in vivo with smooth muscle alpha-actin (alpha-SMA) and smooth muscle myosin heavy chain, we have explored the possible participation of this HDAC in smooth muscle cytoskeleton regulation. Cell fractionation assays performed with primary human smooth muscle cells (HSMCs) showed that HDAC8, in contrast to HDAC1 and HDAC3, was enriched in cytoskeleton-bound protein fractions and insoluble cell pellets, suggesting an association of HDAC8 with the cystoskeleton. Coimmunoprecipitation experiments using HSMCs, NIH-3T3 cells, and human prostate tissue lysates further demonstrated that HDAC8 associates with alpha-SMA but not with beta-actin. HDAC8 silencing through RNA interference strongly reduced the capacity of HSMCs to contract collagen lattices. Mock transfections had no effect on HSMC contractily, and transfections with small interfering RNAs (siRNAs) specific for HDAC6, a cytosolic HDAC that functions as an alpha-tubulin deacetylase, resulted in a weak contraction inhibition. Although mock- and HDAC6 siRNA-transfected HSMCs showed no noticeable morphological changes, HDAC8 siRNA-transfected HSMCs displayed a size reduction with diminished cell spreading after replating. Altogether, our findings indicate that HDAC8 associates with the smooth muscle actin cytoskeleton and may regulate the contractile capacity of smooth muscle cells. [less ▲]

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See detailL'insulinotherapie dans le diabete de type 2.
Philips, Jean-Christophe ULg; Scheen, André ULg

in Revue Médicale de Liège (2005), 60(5-6), 419-23

As compared to type 1 diabetes, type 2 diabetes usually requires insulin at a late stage, after secondary failure to oral antidiabetic drugs. However, it should be pointed out that insulin therapy should ... [more ▼]

As compared to type 1 diabetes, type 2 diabetes usually requires insulin at a late stage, after secondary failure to oral antidiabetic drugs. However, it should be pointed out that insulin therapy should not be delayed in patients not well controlled on oral agents. Type 2 diabetic patients failing to oral antidiabetic medications need insulin. Nevertheless, insulin therapy in type 2 diabetic patients is less standardized than in those with type 1 diabetes. Several clinical trials tried to investigate what is the most appropriate initial insulin therapy in type 2 diabetic patients, with the main objectives of reaching almost normoglycaemia without increased risk of frequent or severe hypoglycaemic episodes. However, there is no agreement upon optimal mode of initiating insulin. The most important factors leading to adequate metabolic control are a global educational approach together with a intensified follow up, independently of the insulin regimen. [less ▲]

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See detailEvaluation des restaurations de pelouses calcicoles en région wallonne: coûts, structure écologique et diversité botanique
Piqueray, Julien ULg; Bottin, Gaëtan; Delescaille, Louis-Marie et al

in Parcs & Réserves (2005), 60(4), 22-35

L’objectif de ce travail était de réaliser une première évaluation des actions de restauration de pelouses calcicoles effectuées dans les régions de Lesse et Lhomme et du Viroin (Belgique) depuis 15 ans ... [more ▼]

L’objectif de ce travail était de réaliser une première évaluation des actions de restauration de pelouses calcicoles effectuées dans les régions de Lesse et Lhomme et du Viroin (Belgique) depuis 15 ans. L’évaluation en terme de structure spatiale a montré que la surface totale de pelouses avait plus que doublé dans ces régions. Cette augmentation était surtout due à l’augmentation de la surface moyenne des pelouses et dans une moindre mesure à l’augmentation du nombre de pelouses présentes sur la zone. L’indice IFM de connectivité entre les pelouses a aussi augmenté. L’évaluation en termes de coûts a permis de montrer que le prix moyens des chantiers de restauration était d’environ 7000 €/ha. Ce prix augmentait avec la pente et la technique de restauration par gyrobroyage permettait de le diminuer là où elle était applicable. L’évolution de la flore suite à la restauration a ensuite été étudiée. Au cours du temps, la végétation sur les parcelles restaurées devenait plus nitrophobe, plus héliophile et contenait proportionnellement plus d’espèces typiques des pelouses, mais dans tous les cas, on n’atteignait pas le niveau des anciennes pelouses qui étaient aussi plus riches en espèces que les pelouses restaurées. L’influence de la nature du peuplement forestier présent avant restauration sur l’évolution de la flore est marquée au début, mais après un temps de 10 à 15 ans, plus aucune différence n’a été retrouvée. La recherche des espèces indicatrices a montré que les restaurations récentes étaient caractérisées par des espèces de coupes et forestières principalement alors que les restaurations anciennes et les pelouses témoins étaient indiquées majoritairement par des espèces de pelouses. Certaines espèces typiques des pelouses calcicoles ne sont pas réapparues sur les pelouses restaurées. [less ▲]

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See detailMaladie thromboembolique périopératoire et obstétricale. Pathologie gynécologique et obstétricale
Benhamou, D.; Mignon, A.; Aya, G. et al

in Annales Françaises d'Anesthésie et de Réanimation (2005), 24

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See detailMature erythrocyte indices: new markers of iron availability.
Bovy, Christophe ULg; Gothot, André ULg; Krzesinski, Jean-Marie ULg et al

in Haematologica (2005), 90(4), 549-51

This study was aimed at evaluating mature erythrocyte indices as new markers of iron status. Contrarily to those in the whole red blood cell (RBC) population, mature erythrocyte parameters are valid ... [more ▼]

This study was aimed at evaluating mature erythrocyte indices as new markers of iron status. Contrarily to those in the whole red blood cell (RBC) population, mature erythrocyte parameters are valid markers of iron status that remain independent of erythropoietic activity. When reticulocytosis is low, these parameters are similar to whole RBC parameters. [less ▲]

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See detailA propos d'un cas d'hémiparésie aigue chez l'enfant
Daron, Aurore; Leroy, Patricia; Misson, Jean-Paul ULg

in Percentile (2005), 10

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See detailImpact de la pollution sur la qualité des légumes à Kinshasa (Pb, As, Hg, Cd).
Delcarte, Emile; Bindelle, Jérôme ULg; Kabamba, Lumpungu

Article for general public (2005)

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See detailImprovement of lupin seed valorisation by the pig with the addition of alpha-galactosidase in the feed and the choice of a suited variety.
Froidmont, E.; Wathelet, Bernard ULg; Beckers, Yves ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2005), 9(4), 225-235

Three experiments were conducted to assess whether an a-galactosidase supplement improved the utilisation of lupin seeds by growing and finishing pigs and to determine the best lupin variety in presence ... [more ▼]

Three experiments were conducted to assess whether an a-galactosidase supplement improved the utilisation of lupin seeds by growing and finishing pigs and to determine the best lupin variety in presence of a-galactosidase. In experiment 1, three iso-nitrogen (iso-N) and iso-net energy (iso-NE) diets containing 15% of soybean meal (SBM diet) or 20% of lupin seeds (Lupinus albus var. Arès), with or without an a-galactosidase supplement (‘lupin’ and ‘lupin + a-gal’ diets, respectively), were ingested by 12 pigs fitted with a caecal canula, following a completely randomized experimental design. The ileal digestibility of a-galactosides tended to increase with the lupin + a-gal diet. Nitrogen retention of pigs eating the SBM diet was similar to that of those fed with the lupin + a-gal diet and higher than that observed for pigs given the lupin diet. The results suggested that a-galactosides are the main anti-nutritional factors in lupin seeds for pigs. The same diets were investigated in a production trial (experiment 2) involving 48 pigs with a live weight of between 30 and 105 kg. The fattening period was similar for the SBM and lupin + a-gal diets (107 d) and longer for the lupin diet (123 d). With the lupin diet there was a lower average daily gain (ADG, -16%) and a higher food conversion ratio (FCR, + 8%) than with the two other diets. The backfat of pigs fed with lupin-based diets contained fewer polyunsaturated fatty acids (PUFA) and had a lower w6/w3 ratio. In experiment 3, three iso-N and iso-NE diets containing different varieties of lupin (Lupinus albus cv Lublanc and Amiga; Lupinus angustifolius cv Boltensia) and an a-galactosidase supplement were investigated in 12 pigs fitted with a caecal canula, as in Exp. 1. The results showed that the digestible protein supply by the Boltensia diet was higher than in the others. However, the Boltensia variety led to a higher urinary N excretion than the Lublanc variety. The results obtained with Amiga were intermediate. Consequently, the utilisation of ingested and digested proteins was relatively high with the Lublanc diet, intermediate with the Amiga diet and low with the Boltensia diet. The results suggested that the Lublanc variety was better adapted for pig feed that contained an a-galactosidase supplement. [less ▲]

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See detailGestion de la qualité physique des bandes riveraines (les berges).
Leboeuf, Dimitri; Marcoen, Jean Marie ULg

Report (2005)

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See detailL’évolution du concept d’imperium
Stasse, Bauduin ULg

in Entretiens sur l’Antiquité gréco-romaine (2005), 27

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See detailFantômes d'Afrique : du "Coup de lune" au "Blanc à lunettes"
Dumortier, Jean-Louis ULg

in Traces (2005), 16

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See detailRéforme de la PAC et avenir de la viande bovine
Burny, Philippe ULg

Diverse speeche and writing (2005)

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See detailContribution à la mise au point de la cDNA-AFLP sur bananes
Lassois, Ludivine ULg

Master of advanced studies dissertation (2005)

Obtaining ARN of quality and in sufficient quantity is an essential stage in the realization of cDNA-AFLP. The effectiveness of several protocols of RNA extraction was evaluated on peel and pulp of green ... [more ▼]

Obtaining ARN of quality and in sufficient quantity is an essential stage in the realization of cDNA-AFLP. The effectiveness of several protocols of RNA extraction was evaluated on peel and pulp of green and yellow bananas. On peel and pulp of green bananas, 4 protocols were tested and two of them allowed obtaining RNA of good quality and in sufficient quantity. They acts of the protocol described by Liu and al. (1998) and that proposed by the CIRAD (not published) and which is a modified version of Wan and Wilkins (1994). However, new tests must be realized in order to choose more adapted in the case of the extractions on peel and pulp of green bananas. Seven protocols were tested and compared on peel and pulp of yellow bananas. Among those, the protocols describes by Liu and al. (1998) and that proposed by the CIRAD (not published) gave the best results. [less ▲]

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See detailDetection and epidemiological characteristics of peach latent mosaic viroid in Tunisia.
Hassen, I. F.; Roussel, S.; Kummert, J. et al

in Communications in Agricultural and Applied Biological Sciences (2005), 70(3), 115-128

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See detailEtayer plus pour remédier moins
Dumortier, Jean-Louis ULg

in Education-Formation (2005), 280

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See detailPerception of aphid infested tomato plant volatiles by the predator Episyrphus balteatus
Verheggen, François ULg; Arnaud, Ludovic; Capella, Quentin et al

in Communications in Agricultural and Applied Biological Sciences (2005)

In a tritrophic interaction including tomato plant (Lycopersicon esculentum Miller), the herbivore Myzus persicae (Sulzer) and the predator Episyrphus balteatus (De Geer), the perception of the tomato ... [more ▼]

In a tritrophic interaction including tomato plant (Lycopersicon esculentum Miller), the herbivore Myzus persicae (Sulzer) and the predator Episyrphus balteatus (De Geer), the perception of the tomato plants produced volatile organic compounds (VOC) by Episyrphus balteatus is investigated. In a first step, an odour sampling device has been set up aiming the headspace collection of the tomato plant VOC and their adsorption on Tenax adsorbent cartridges (Supelco®). Following desorption is held using a thermodesorption injector (Gerstel®) coupled with GC-MS. Intact and aphid infested plants are studied for their VOC emissions, as well as the comparison of the VOC emission of different tomato cultivars. These VOC consist mainly in mono- and sesquiterpenes (such as - and -pinene ; -humulene ; …) as well as in C6-volatiles like hexenal in case of infestation by herbivores Once the tomato plants VOC identified and quantified, they are tested for their perception by Episyrphus balteatus using electroantennography (EAG). Accordingly, an EAG device has been installed and configured for the study of VOC using Diptera antennas. The monoterpenes limonene and linalool showed high EAG activity whereas other terpenes like cymene seem to be inactive. [less ▲]

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See detailRéponse à : Chirurgie de l'incontinence urinaire a l'effort feminine par voie transobturatrice: dehors dedans ou dedans dehors? etude anatomique comparative.
de Leval, Jean ULg; Bonnet, Pierre ULg; Waltregny, David ULg et al

in Progrès en Urologie : Journal de l'Association Française d'Urologie et de la Société Française d'Urologie (2005), 15(6), 1161-2

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See detailL’organisation de la formation agricole au benin : quels nouveaux roles pour les differents acteurs dans la mise en oeuvre de nouvelles politiques de formation des producteurs ruraux
Tossou, Cocou Rigobert; Assogba, Sonagnon Claude-Gervais ULg; Vodouhê, Davo Simplice

in Annales des Sciences Agronomiques du Bénin (2005), 7(1),

L'objectif de cet article est d'analyser les dispositifs actuels de formation des producteurs ruraux au Bénin afin de comprendre la manière dont les programmes de formation sont conçus et mis en oeuvre ... [more ▼]

L'objectif de cet article est d'analyser les dispositifs actuels de formation des producteurs ruraux au Bénin afin de comprendre la manière dont les programmes de formation sont conçus et mis en oeuvre pour en dégager des enseignements pour de nouvelles politiques de formation dans le domaine. Trois structures ont servi de cas d'étude : le Centre Régional pour la Promotion Agricole de l’Ouémé (CeRPA-O), le Programme de Technologie Agricole et Alimentaire (PTAA) et l'Union Départementale des Producteurs de l’Ouémé (UDP-O). Au niveau de ces structures, 90 acteurs de la formation paysanne ont été sélectionnés. Les analyses se sont basées aussi bien sur les principes d'andragogie que sur la démarche d'Ingénierie des Dispositifs de Formation (DIF). Des investigations et analyses, il ressort que les formations offertes aux ruraux sont compatibles avec leurs besoins en formation mais l'élaboration de ces programmes est caractérisée par une forte implication des intervenants extérieurs au détriment d’une participation active des producteurs. Les méthodes d'apprentissage utilisées ne sont pas toujours compatibles aux principes de l'éducation des adultes. Par ailleurs, les formateurs disposent des compétences techniques mais ne maîtrisent pas souvent les capacités andragogiques requises. Le financement des formations repose pour l’essentiel sur l’extérieur, ce qui rend peu viables ces dispositifs de formation. Enfin, les politiques d'accompagnement des formations mises en oeuvre demeurent insuffisantes pour une mise en pratique effective et durable des acquis de la formation. D'où la nécessité de concevoir des programmes de formation qui tiennent compte du contexte politico-économique, de l'andragogie et de la Démarche Ingénierie de Formation. [less ▲]

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