References of "2004"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailCardiomyocyte-restricted overexpression of endothelial nitric oxide synthase (NOS3) attenuates beta-adrenergic stimulation and reinforces vagal inhibition of cardiac contraction.
MASSION, Paul ULg; Dessy, Chantal; Desjardins, Fanny et al

in Circulation (2004), 110(17), 2666-72

BACKGROUND: In the heart, nitric oxide synthases (NOS) modulate cardiac contraction in an isoform-specific manner, which is critically dependent on their cellular and subcellular localization. Defective ... [more ▼]

BACKGROUND: In the heart, nitric oxide synthases (NOS) modulate cardiac contraction in an isoform-specific manner, which is critically dependent on their cellular and subcellular localization. Defective NO production by NOS3 (endothelial NOS [eNOS]) in the failing heart may precipitate cardiac failure, which could be reversed by overexpression of NOS3 in the myocardium. METHODS AND RESULTS: We studied the influence of NOS3 in relation to its subcellular localization on the function of cardiomyocytes isolated from transgenic mice overexpressing NOS3 under the alpha-myosin heavy chain promoter (NOS3-TG). Immunoblot analysis demonstrated moderate (5-fold) NOS3 overexpression in cardiomyocytes from NOS3-TG heterozygotes. Caveolar localization of transgenic eNOS was demonstrated by immunofluorescence, coimmunoprecipitation with caveolin-3, sucrose gradient fractionation, and immunogold staining revealed by electron microscopy. Compared with wild-type littermate, contractility of NOS3-TG cardiomyocytes analyzed by videomicroscopy revealed a lower incidence of spontaneous arrhythmic contractions (n=32, P<0.001); an attenuation of the beta-adrenergic positive inotropic response (isoproterenol, 10(-7) mol/L: 62.1+/-7.8% versus 90.8+/-8.0% of maximum Ca2+ response; n=10 to 17; P<0.05); a potentiation of the muscarinic negative chronotropic response (carbamylcholine, 3.10(-8) mol/L: -63.9+/-14% versus -27.7+/-5.6% of basal rate; n=8 to 10; P<0.05), confirmed by telemetry in vivo; and an attenuation of the accentuated antagonism of beta-adrenergically stimulated contraction (-14.6+/-1.5% versus -3.5+/-1.5; n=7 to 11; P<0.05). Cardiomyocyte NOS inhibition reversed all 4 effects (P<0.05). CONCLUSIONS: Moderate overexpression of NOS3, targeted to caveolae in murine cardiomyocytes, potentiates the postsynaptic muscarinic response and attenuates the effect of high concentrations of catecholamines. Cardiomyocyte NOS3 may represent a promising therapeutic target to restore the sympathovagal balance and protect the heart against arrhythmia. [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailConservation management for Orthoptera in the Dadia reserve, Greece
Kati, V.; Dufrêne, Marc ULg; Legakis, A. et al

in Biological Conservation (2004), 115(1), 33-44

The diversity patterns, the ecological structure and the typical species of the orthopteran assemblage in the Dadia reserve are investigated. The reserve was designed to protect the black vulture ... [more ▼]

The diversity patterns, the ecological structure and the typical species of the orthopteran assemblage in the Dadia reserve are investigated. The reserve was designed to protect the black vulture (Aegypius monachus) and other raptors. A total of 39 orthopteran species were found, including Paranocarodes chopardi, a pamphagid species with very restricted distribution. All species can be represented in a network of six complementary habitats, including open oak woodlands, agricultural fields separated with hedges, humid grasslands, as well as serpentine grasslands. The buffer zone of the reserve is far more important for Orthoptera conservation than the core areas, which host most of the black vulture nests. Management focusing on raptors is in general compatible with conservation of Orthoptera. We suggest the maintenance of forest openings in the buffer zone, the maintenance of forest heterogeneity, the enhancement of periodical livestock grazing, and the use of nine indicator species and Paranocarodes chopardi in the reserve monitoring program. © 2003 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 13 (2 ULg)
Full Text
See detailL'anémie: facteur de risque cardio-vasculaire?
Krzesinski, Jean-Marie ULg

in Journal de Cardiologie [= JDC] = Tijdschrift voor Cardiologie [= TVC] (2004), 16(2), 53-62

Detailed reference viewed: 10 (3 ULg)
See detailÉvaluation des compétences en TIC
Vandeput, Etienne ULg

in André, Bernard; Baron, Georges-Louis; Bruillard, Éric (Eds.) Traitement de texte et production de documents, questions didactiques (2004)

Divers systèmes de certification des compétences en TIC sont décrits et comparés. L'article tente de mettre en lumière les qualités et les défauts de chacun et propose une stratégie pour la construction d ... [more ▼]

Divers systèmes de certification des compétences en TIC sont décrits et comparés. L'article tente de mettre en lumière les qualités et les défauts de chacun et propose une stratégie pour la construction d'une certification dans la durée. [less ▲]

Detailed reference viewed: 35 (5 ULg)
Full Text
See detailPropriété et union libre:vers un affaiblissement de la preuve par le titre
Dehalleux, Virginie ULg

in Revue Générale de Droit Civil Belge = Tijdschrift voor Belgisch Burgerlijk (2004)

Detailed reference viewed: 8 (1 ULg)
See detailHow dangerous is the environment for the breast?
Charlier, Corinne ULg

Conference (2004)

Detailed reference viewed: 2 (0 ULg)
Full Text
See detailDéveloppement : la course à la compétitivité
Lebailly, Philippe ULg

Article for general public (2004)

Detailed reference viewed: 32 (8 ULg)
Full Text
Peer Reviewed
See detailVraies et fausses éditions liégeoises ou rouennaises de Voltaire
Droixhe, Daniel ULg

in Revue Voltaire (2004), 4

Detailed reference viewed: 9 (0 ULg)
See detailLlandovery biostratigraphy of the Paraná Basin, East Paraguay
Mendlowicz Mauller, P.; Pereira, E.; Grahn, Y. et al

Conference (2004)

Detailed reference viewed: 4 (0 ULg)
Full Text
See detailApproche théorique et pratique du délit d'abandon de famille
Collin, Jonathan ULg

Master's dissertation (2004)

Detailed reference viewed: 36 (7 ULg)
See detailExecutive functions
Collette, Fabienne ULg; Van der Linden, Martial ULg

in Morris, R. G.; Becker, J. T. (Eds.) The cognitive Neuropsychology of Alzheimer's disease (2004)

Detailed reference viewed: 30 (8 ULg)
Full Text
Peer Reviewed
See detailCase studies in measurement of random incidence scattering coefficients
Vorländer, Michael; Embrechts, Jean-Jacques ULg; De Geetere, Lieven et al

in Acta Acustica United With Acustica (2004), 90(5, SEP-OCT), 858-867

In this work measurements of random-incidence scattering coefficients in three laboratories are compared. The sample geometry is sinusoidal. These surfaces are geometrically identical, but they were ... [more ▼]

In this work measurements of random-incidence scattering coefficients in three laboratories are compared. The sample geometry is sinusoidal. These surfaces are geometrically identical, but they were constructed in different scales. So far, measurements of this kind were performed only in scale models. Using turntables in real sample size was hardly considered possible. One result of general importance is that measurements are indeed possible in real-scale reverberation rooms with turntables of 3 m diameter. There are only small differences between the real-scale and model-scale results. Some variations of the standard procedure were tested and the uncertainties identified: mounting of the sample, connection or sealing between sample and base plate, way of rotating the sample, air absorption and time variances. The results presented can be regarded as guidelines for application of ISO/DIS 17497-1. [less ▲]

Detailed reference viewed: 47 (4 ULg)
See detailValutazione del transessualismo primario e secondario tramite il Rorschach comprehensive system
Porcelli, P.; Affatati, V.; Michel, Alexa ULg et al

in Psicologia Medica e Psicoterapia (2004), 23

Detailed reference viewed: 9 (3 ULg)
See detailTo build equity indicators in a European Context
Baye, Ariane ULg

Conference given outside the academic context (2004)

Full Text
Peer Reviewed
See detailFemmes qui rêvent avec les doigts, les voies de la peinture aborigènes d'Australie
Strivay, Lucienne ULg

in Cahiers Internationaux de Symbolisme (2004), 107-108-109

Detailed reference viewed: 27 (0 ULg)
Full Text
Peer Reviewed
See detailPartial Atomic Charges Of Amino Acids In Proteins
Thomas, Annick ULg; Milon, A.; Brasseur, Robert ULg

in Proteins-Structure Function and Bioinformatics (2004), 56(1), 102-9

Using a semiempirical quantum mechanical procedure (FCPAC) we have calculated the partial atomic charges of amino acids from 494 high-resolution protein structures. To analyze the influence of the protein ... [more ▼]

Using a semiempirical quantum mechanical procedure (FCPAC) we have calculated the partial atomic charges of amino acids from 494 high-resolution protein structures. To analyze the influence of the protein's environment, we considered each residue under two conditions: either as the center of a tripeptide with PDB structure geometry (free) or as the center of 13-16 amino acid clusters extracted from the PDB structure (buried). The partial atomic charges from residues in helices and in sheets were separated. The FCPAC partial atomic charges of the Cbeta and Calpha of most residues correlate with their helix propensity, positively for Cbeta and negatively for Calpha (r2 = 0.76 and 0.6, respectively). The main consequence of burying residues in proteins is the polarization of the backbone C=O bond, which is more pronounced in helices than in sheets. The average shift of the oxygen partial charges that results from burying is -0.120 in helix and -0.084 in sheet with the charge of the proton as unit. Linear correlations are found between the average NMR chemical shifts and the average FCPAC partial charges of Calpha (r2 = 0.8-0.85), N (r3 = 0.67-0.72), and Cbeta (r2 = 0.62) atoms. Correlations for helix and beta-sheet FCPAC partial charges show parallel regressions, suggesting that the charge variations due to burying in proteins differentiate between the dihedral angle effects and the polarization of backbone atoms. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailIntrogression in tetraploid cotton of the resistance to reniform nematode Rotylenchulus reniformis Lind. & Oliveira from the Gossypium hirsutum L. x G. longicalyx Hutch & Lee x G. thurberi Tod trispecific hybrid
Konan, N'Guessan ULg; Ruano, O.; Prud'Homme, S. et al

in World Cotton Research Conference - 3. Cotton production for the new Millenium (2004)

In order to develop upland cotton plants resistant to Rotylenchulus reniformis Lind. & Oliveira reniform nematode, a three-species hybrids including Gossypium hirsutum L. (2n = 4 x = 52, (AD)1 genome) was ... [more ▼]

In order to develop upland cotton plants resistant to Rotylenchulus reniformis Lind. & Oliveira reniform nematode, a three-species hybrids including Gossypium hirsutum L. (2n = 4 x = 52, (AD)1 genome) was created using G. longicalyx Hutch & Lee as donor parent (2n = 2x = 26, F1 genome) and G. thurberi Tod. (2n = 2x = 26, D1 genome) as bridge species. The morphology and the resistance to reniform nematode of the parents and of thirty plants belonging to the selfed progeny of G. hirsutum x G. longicalyx x G. thurberi (HLT) trispecific hybrid were assessed. G. longicalyx, G. hirsutum x G. longicalyx hexaploid and all the thirty plants issued from the selfing of HLT hybrid were very resistant to reniform nematode unlike G. hirsutum which was very sensitive to it. Cytogenetic studies showed that HLT hybrid and its direct progeny have 52 chromosomes as the main cotton cultivated species. HLT hybrid shows a good pollen fertility and a very high level of chromosome pairing at metaphase I. It is self fertile but presents important incompatibility barriers when crossed as female parent with G. hirsutum. The prospects to develop upland cotton commercial cultivars resistant to reniform nématodes from the available interspecific genetic stocks are discussed. [less ▲]

Detailed reference viewed: 59 (2 ULg)