References of "2004"
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See detailHormonal regulation of brain circuits mediating male sexual behavior in birds
Ball, G. F.; Balthazart, Jacques ULg

in Physiology & Behavior (2004), 83(2), 329-346

Male sexual behavior in both field and laboratory settings has been studied in birds since the 19th century. Birds are valuable for the investigation of the neuroendocrine mechanisms of sexual behavior ... [more ▼]

Male sexual behavior in both field and laboratory settings has been studied in birds since the 19th century. Birds are valuable for the investigation of the neuroendocrine mechanisms of sexual behavior, because their behavior can be studied in the context of a large amount of field data, well-defined neural circuits related to reproductive behavior have been described, and the avian neuroendocrine system exhibits many examples of marked plasticity. As is the case in other taxa, male sexual behavior in birds can be usefully divided into an appetitive phase consisting of variable behaviors (typically searching and courtship) that allow an individual to converge on a functional outcome, copulation (consummatory phase). Based primarily on experimental studies in ring doves and Japanese quail, it has been shown that testosterone of gonadal origin plays an important role in the activation of both of these aspects of male sexual behavior. Furthermore, the conversion of androgens, such as testosterone, in the brain to estrogens, such as 17beta-estradiol, is essential for the full expression of male-typical behaviors. The localization of sex steroid receptors and the enzyme aromatase in the brain, along with lesion, hormone implant and immediate early gene expression studies, has identified many neural sites related to the control of male behavior. The preoptic area (POA) is a key site for the integration of sensory inputs and the initiation of motor outputs. Furthermore, prominent connections between the POA and the periaqueductal gray (PAG) form a node that is regulated by steroid hormones, receive sensory inputs and send efferent projections to the brainstem and spinal cord that activate male sexual behaviors. The sensory inputs regulating avian male sexual responses, in contrast to most mammalian species, are primarily visual and auditory, so a future challenge will be to identify how these senses impinge on the POA-PAG circuit. Similarly, most avian species do not have an intromittent organ, so the projections from the POA-PAG to the brainstem and spinal cord that control sexual reflexes will be of particular interest to contrast with the well characterized rodent system. With this knowledge, general principles about the organization of male sexual circuits can be elucidated, and comparative studies relating known species variation in avian male sexual behaviors to variation in neural systems can be pursued. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

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See detailHarmonization of strategies for the validation of quantitative analytical procedures - A SFSTP proposal - part I
Hubert, Philippe ULg; Nguyen-Huu, J.-J.; Boulanger, Bruno ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2004), 36(3), 579-586

This paper is the first part of a summary report of a new commission of the Societe Francaise des Sciences et Techniques Pharmaceutiques (SFSTP). The main objective of this commission was the ... [more ▼]

This paper is the first part of a summary report of a new commission of the Societe Francaise des Sciences et Techniques Pharmaceutiques (SFSTP). The main objective of this commission was the harmonization of approaches for the validation of quantitative analytical procedures. Indeed, the principle of the validation of theses procedures is today widely spread in all the domains of activities where measurements are made. Nevertheless. this simple question of acceptability or not of an analytical procedure for a given application, remains incompletely determined in several cases despite the various regulations relating to the good practices (GLP, GMP,...) and other documents of normative character (ISO, ICH. FDA,...). There are many official documents describing the criteria of validation to be tested, but they do not propose any experimental protocol and limit themselves most often to the general concepts. For those reasons, two previous SFSTP commissions elaborated validation guides to concretely help the industrial scientists in charge of drug development to apply those regulatory recommendations. If these two first guides widely contributed to the use and progress of analytical validations, they present, nevertheless, weaknesses regarding the conclusions of the performed statistical tests and the decisions to be made with respect to the acceptance limits defined by the use of an analytical procedure. The present paper proposes to review even the bases of the analytical validation for developing harmonized approach, by distinguishing notably the diagnosis rules and the decision rules. This latter rule is based on the use of the accuracy profile, uses the notion of total error and allows to simplify the approach of the validation of an analytical procedure while checking the associated risk to its usage. Thanks to this novel validation approach, it is possible to unambiguously demonstrate the fitness for purpose of a new method as stated in all regulatory documents. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailPolitiques sportives pour la "non violence"
Comeron, Manuel ULg

Conference (2004, November 14)

ÁREAS TEMÁTICAS: 1. MEDIOS DE COMUNICACIÓN (Radiotelevisión y Prensa), ASOCIACIONISMO DEPORTIVO Y CLUBES DE LIGAS FEDERADAS. 2. ACADÉMICA Y EDUCATIVA (EL DEPORTE, LA VIOLENCIA Y LOS PROCESOS DE ... [more ▼]

ÁREAS TEMÁTICAS: 1. MEDIOS DE COMUNICACIÓN (Radiotelevisión y Prensa), ASOCIACIONISMO DEPORTIVO Y CLUBES DE LIGAS FEDERADAS. 2. ACADÉMICA Y EDUCATIVA (EL DEPORTE, LA VIOLENCIA Y LOS PROCESOS DE CIVILIZACIÓN : Antropología del Deporte, Psicología eEolutiva de la Actividad Física y Sociología y del Deporte) 3. LEGISLATIVA (LEGISLACIÓN EN EL ÁMBITO DE LA VIOLENCIA DEPORTIVA) 4. LAS POLÍTICAS DEPORTIVAS PARA LA “NOVIOLENCIA” (VIOLENCIA DESDE EL PUNTO DE VISTA DEL ENTORNO / MODELO INTERNACIONAL, NACIONAL Y AUTONÓMICO) [less ▲]

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See detailAnálisis cladístico de la correlación entre patogenia y marcadores moleculares en la región 5’ LTR del Virus de la Leucosis Bovina
Rodriguez, Sabrina ULg; Zwerdling, A.; Jones, L.R. et al

Poster (2004, November 14)

ANALISIS CLADISTICO DE LA CORRELACION ENTRE PATOGENIA Y MARCADORES MOLECULARES EN LA REGION 5’ LTR DEL VIRUS DE LA LEUCOSIS BOVINA. SM Rodriguez1; A Zwerdling1; LR Jones1; K Trono1 1Instituto de Virología ... [more ▼]

ANALISIS CLADISTICO DE LA CORRELACION ENTRE PATOGENIA Y MARCADORES MOLECULARES EN LA REGION 5’ LTR DEL VIRUS DE LA LEUCOSIS BOVINA. SM Rodriguez1; A Zwerdling1; LR Jones1; K Trono1 1Instituto de Virología, CICVyA, INTA-Castelar. CC 25 (1712), Castelar, Argentina. El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un miembro de la familia Retroviridae. Esta familia incluye los virus responsables de la leucemia de células T de los humanos (Human T-Cell Lymphotropic Virus-1 y -2) y de los simios (Simian T-lymhotropic Virus). BLV afecta al ganado bovino produciendo en un reducido numero de animales (2-5%) una neoplasia linfoproliferativa de células B de origen clonal conocida como Leucosis Bovina Enzootica (LBE). La mayoría de los individuos infectados con BLV son portadores asintomáticos (AL), cursando la infección sin signos clínicos aparentes. Alrededor del 30% de los animales infectados desarrolla una proliferación benigna policlonal de células B, denominada Linfocitosis Persistente (LP). En nuestro laboratorio hemos secuenciado la zona R-U5 del sector 5' LTR de aislamientos provenientes de 36 animales presentando diferentes formas clínicas. El resultado de estos estudios reveló la presencia de 4 bases covariantes para provirus aislados de linfosarcoma (LS). Estas modificaciones están ausentes en la mayoría (16/22) de los provirus aislados de formas asintomáticas (AL/PL). Si bien esta aparente correlación entre los cambios de nucleótidos y los cuadros clínicos sugiere la presencia de marcadores de patogenia, es necesario analizar dentro de un contexto histórico (cladograma) la aparición independiente de dicho marcador en conjunto con el desarrollo de LS. Para ello, se obtuvieron las secuencias correspondientes a la región genómica codificante env y pX para algunos de estos aislamientos de LS y AL/PL. Las secuencias fueron alineadas mediante el programa ClustalX y los alineamientos fueron utilizados para obtener cladogramas mediante las rutinas de máxima parsimonia del programa PAUP*. El análisis de los árboles obtenidos sugiere que la adquisición del marcador en la región LTR y el desarrollo de LS en diferentes aislamientos serían independientes, apoyando la hipótesis de la existencia de un marcador de patogenia en dicha zona regulatoria. [less ▲]

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See detailExpresión de la proteína p24 del Virus de la Leucosis Bovina en E. coli y su potencial utilización en pruebas de diagnostico
Gutiérrez, G.; Álvarez, I.; Rodriguez, Sabrina ULg et al

Poster (2004, November 14)

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See detailLe pilotage du système éducatif via des indicateurs
Baye, Ariane ULg

Conference given outside the academic context (2004)

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See detailFully automated method for the liquid chromatographic-tandem mass spectrometric determination of cyproterone acetate in human plasma using restricted access material for on-line sample clean-up
Christiaens, B.; Fillet, Marianne ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (2004), 1056(1-2), 105-110

A new automated method for the quantitative analysis of cyproterone acetate (CPA) in human plasma has been developed using on-line solid phase extraction (SPE) prior to the LC-MS/MS determination. The ... [more ▼]

A new automated method for the quantitative analysis of cyproterone acetate (CPA) in human plasma has been developed using on-line solid phase extraction (SPE) prior to the LC-MS/MS determination. The method was based on the use of a pre-column packed with internal-surface reversed-phase material (LiChrospher RP-4 ADS, 25 mm x 2 mm) for sample clean-up coupled to LC separation on an octadecyl silica stationary phase by means of a column switching system. A 30 microl plasma sample volume was injected directly onto the pre-column using a mixture of water, acetonitrile and formic acid (90:10:0.1 (v/v/v)) adjusted to pH 4.0 with diluted ammonia as washing liquid. The analyte was then eluted in the back-flush mode with the LC mobile phase consisting of water, methanol and formic acid (10:90:0.1 (v/v/v)). The dispensing flow rates of the washing liquid and the LC mobile phase were 300 microl min(-1). Medroxyprogesterone acetate (MPA) was used as internal standard. The MS ionization of the analytes was achieved using electrospray (ESI) in the positive ion mode. The pseudomolecular ionic species of CPA and MPA (417.4 and 387.5) were selected to generate daughter ions at 357.4 and 327.5, respectively. Finally, the developed method was validated according to a new approach using accuracy profiles as a decision tool. Very good results with respect to accuracy, detectability, repeatability, intermediate precision and selectivity were obtained. The LOQ of cyproterone acetate was 300 pg ml(-1). [less ▲]

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See detailGestion globale du traitement de l'hypertension artérielle
Krzesinski, Jean-Marie ULg

Conference (2004, November 12)

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See detailNumerical simulation of springback using enhanced assumed strain elements
Bui, Q. V.; Papeleux, Luc ULg; Ponthot, Jean-Philippe ULg

in Journal of Materials Processing Technology (2004), 153(1), 314-318

The quality of springback prediction for a sheet metal forming process depends on a precise estimate of the elasto-plastic stress distribution throughout the metal sheet. The use of low-order conventional ... [more ▼]

The quality of springback prediction for a sheet metal forming process depends on a precise estimate of the elasto-plastic stress distribution throughout the metal sheet. The use of low-order conventional finite elements may be, without any proper treatment, responsible for low quality prediction because of volumetric and shear lockings. In this study, the enhanced assumed strain technique will be exploited for locking removal. The quality of the numerical simulation is evaluated through a comparison with other popular techniques like selective and uniform reduced integration. In contrast to the latter, and thanks to a full numerical integration scheme, the enhanced assumed strain element is very efficient in accurately capturing the development of plastic flow. This enables a reliable prediction of springback even with a rather coarse mesh. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailEfficient algorithmic approach for mixed-lubrication in cold rolling
Stephany, A.; Ponthot, Jean-Philippe ULg; Collette, C. et al

in Journal of Materials Processing Technology (2004), 153(1), 307-313

A model of cold rolling taking into account lubrication in mixed mode was developed. The main objective is to obtain a numerical tool that permits us to optimise a mill configuration from the lubrication ... [more ▼]

A model of cold rolling taking into account lubrication in mixed mode was developed. The main objective is to obtain a numerical tool that permits us to optimise a mill configuration from the lubrication point of view. It means that lubricant viscosity but also roll diameter and roughness, rolling diagrams, etc could be modified to improve stability and efficiency of the mill. A modified slab method is solved iteratively and is coupled with a sophisticated tribological model. A general calculation of the modified Reynolds equation is presented in order to evaluate the fluid pressure for any piezoviscosity law. The coupling is made through the calculation of fluid thickness and friction along the roll bite. The evolution of the cylinder/strip contact area due to the asperity crushing along the roll bite is also considered. A parametric study has been undertaken in order to determine the influence of various process parameters on friction and lubricant flow. Applications to industrial cases obtained from Cockerill Sambre plant will show the applicability of the method. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailParticle size analysis : a multidimensional approach
Grandfils, Christian ULg

Conference (2004, November 09)

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See detailEquity indicators in the field of continuous education and training
Baye, Ariane ULg

Scientific conference (2004, November 08)

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See detailLe syndrome de Parsonage et Turner
Kaux, Jean-François ULg; Wang, François ULg

Conference (2004, November 02)

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See detailOptical polarization of 203 QSOs (Sluse+, 2005)
Sluse, Dominique ULg; Hutsemekers, Damien ULg; Lamy, H. et al

Textual, factual or bibliographical database (2004)

This Table contains linear polarization measurements (mainly in the V band) of 203 QSOs. First time measurements have been obtained for 184 objects. Among them 109 known radio emitters, 42 BAL and 1 ... [more ▼]

This Table contains linear polarization measurements (mainly in the V band) of 203 QSOs. First time measurements have been obtained for 184 objects. Among them 109 known radio emitters, 42 BAL and 1 gravitationally lensed QSO. (1 data file). [less ▲]

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See detailStructural organization of mitochondrial human complex I: role of the ND4 and ND5 mitochondria-encoded subunits and interaction with prohibitin
Bourges, I.; Ramus, C.; de Camaret, B. M. et al

in Biochemical Journal (2004), 383(Part 3), 491-499

Mitochondria-encoded ND (NADH dehydrogenase) subunits, as components of the hydrophobic part of complex I, are essential for NADH:ubiquinone oxidoreductase activity. Mutations or lack of expression of ... [more ▼]

Mitochondria-encoded ND (NADH dehydrogenase) subunits, as components of the hydrophobic part of complex I, are essential for NADH:ubiquinone oxidoreductase activity. Mutations or lack of expression of these subunits have significant pathogenic consequences in humans. However, the way these events affect complex I assembly is poorly documented. To understand the effects of particular mutations in ND subunits on complex I assembly, we studied four human cell lines: ND4 non-expressing cells, ND5 non-expressing cells, and rhodegrees cells that do not express any ND subunits, in comparison with normal complex I control cells. In control cells. all the seven analysed nuclear-encoded complex I subunits Were found to be attached to the mitochondrial inner membrane, except for the 24 kDa subunit, which was nearly equally partitioned between the membranes and the matrix. Absence of a single ND subunit, or even all the seven ND subunits, caused no major changes in the nuclear-encoded complex I subunit content of mitochondria. However, in cells lacking ND4 or ND5, very low amounts of 24 kDa subunit were found associated with the membranes, whereas most of the other nuclear-encoded subunits remained attached. In contrast, membrane association of most of the nuclear subunits was significantly reduced in the absence of all seven ND proteins. Immunopurification detected several subcomplexes. One of these, containing the 23, 30 and 49 kDa subunits, also contained prohibitin. This is the first description of prohibitin interaction with complex I subunits and suggests that this protein might play a role in the assembly or degradation of mitochondrial complex I. [less ▲]

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See detailMeasurement of selected polybrominated diphenyl ethers, polybrominated and polychlorinated biphenyls, and organochlorine pesticides in human serum and milk using comprehensive two-dimensional gas chromatography isotope dilution time-of-flight mass spectrometry
Focant, Jean-François ULg; Sjodin, Andreas; Turner, Wayman E. et al

in Analytical Chemistry (2004), 76(21), 6313-6320

A new method using comprehensive two-dimensional gas chromatography and isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) for the simultaneous measurement of selected polychlorinated ... [more ▼]

A new method using comprehensive two-dimensional gas chromatography and isotope dilution time-of-flight mass spectrometry (GC x GC-IDTOFMS) for the simultaneous measurement of selected polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and brominated flame retardants is presented. In contrast to the reference methods based on classical GC/MS, a single injection of the extract containing all compounds of interest results in accurate identification and quantification. Using GC x GC ensures the chromatographic separation of most compounds, and TOFMS allows mass spectral deconvolution of coeluting compounds as well as the use of C-13-labeled internal standards for quantification. Isotope ratio measurements of the most intense ions for both native and labels ensure the required specificity. The use of this new method with an automated sample preparation procedure developed at the Centers for Disease Control and Prevention (CDC) for the analysis of human serum and milk compared favorably to conventional isotope-dilution one-dimensional gas chromatography-high-resolution mass spectrometty (GC-IDHRMS) for the different human serum and milk pools tested. The instrumental detection limits ranged between 0.5 pg/muL and 10 pg/muL and the method detection limits ranged between I and 15 pg/muL (N = 59 analytes). The reproducibility of the method was almost as good as with GC-IDHRMS, the relative standard deviations ranging between 1 and 11% for OCPs measured in human serum. OCP, PBDE, and PCB levels measured using the two methods were highly correlated, and the deviations between the two methods were below 20% for most analytes with concentrations above 1 ng/g milk lipids. [less ▲]

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