References of "2003"
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See detail8-Chloro-5-(4-methylpiperazin-1-yl)-11H-pyrido[2,3-b][1,5]benzoxazepine
Dupont, Léon ULg; Liégeois, Jean-François ULg

in Acta Crystallographica Section E-Structure Reports Online (2003), 59(Part 12), 1962-1963

The crystal structure of the title compound, C17H17ClN4O, has been undertaken as part of our studies of dopamine receptors. The oxazepine ring has a boat conformation, while the piperazine ring is in a ... [more ▼]

The crystal structure of the title compound, C17H17ClN4O, has been undertaken as part of our studies of dopamine receptors. The oxazepine ring has a boat conformation, while the piperazine ring is in a normal chair conformation. The dihedral angle between the two aromatic rings that lie on the same side of the oxazepine moiety is 113.99 (7)degrees. There is no hydrogen bonding. [less ▲]

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See detailIntrahepatic synthesis of tumor necrosis factor-alpha related to cardiac surgery is inhibited by interleukin-10 via the Janus kinase (Jak)/signal transducers and activator of transcription (STAT) pathway.
Qing, Ma; Nimmesgern, Ariane; Heinrich, Peter C et al

in Critical Care Medicine (2003), 31(12), 2769-75

SUMMARY: OBJECTIVES To identify the signaling pathways involved in the anti-inflammatory shift of the cytokine balance due to hypothermia during cardiopulmonary bypass. DESIGN Experimental animal study ... [more ▼]

SUMMARY: OBJECTIVES To identify the signaling pathways involved in the anti-inflammatory shift of the cytokine balance due to hypothermia during cardiopulmonary bypass. DESIGN Experimental animal study. SETTING Department of experimental surgery of a university hospital. SUBJECTS Young pigs. INTERVENTIONS Animals underwent normothermic (37 degrees C) or hypothermic (28 degrees C) cardiopulmonary bypass (n = 6 each). Samples of liver tissue were taken before and 6 hrs after cardiopulmonary bypass. MEASUREMENTS AND MAIN RESULTS Intrahepatic expression of tumor necrosis factor-alpha, interleukin-10, inducible nitric oxide synthase, and suppressor of cytokine signaling-3 was detected by reverse transcriptase polymerase chain reaction and/or Western blotting. Concentrations of the inhibitory protein of nuclear factor-kappaB, IkappaB, and of the signal transducer and activator of transcription (STAT)-3 were measured by Western blotting. The DNA-binding activity of nuclear factor-kappaB and STAT-3 was assessed by electrophoretic mobility shift and supershift assays. Liver cell necrosis and apoptosis were assessed by histology and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay, respectively. Pigs operated on in hypothermia showed significantly higher intrahepatic concentrations of interleukin-10 and lower concentrations of tumor necrosis factor-alpha than the others. They also showed a lower percentage of hepatic cell necrosis but not of apoptosis. This anti-inflammatory reaction observed in the hypothermic group was associated with a higher expression of suppressor of cytokine signaling-3 and with increased activation of STAT-3. Activation of nuclear factor-kappaB and expression of inducible nitric oxide synthase, however, were not significantly different between both groups. CONCLUSION Our results show that hypothermia during cardiopulmonary bypass up-regulates interleukin-10 via STAT-3 activation, which in turn leads to the attenuation of tumor necrosis factor-alpha expression and to hepatic protection. [less ▲]

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See detailA capillary action test for the investigation of adhesion in repair technology
Courard, Luc ULg; Degeimbre, Robert ULg

in Canadian Journal of Civil Engineering (2003), 30(6), 1101-1110

When repair material is laid down on a concrete substrate, the porosity of concrete allows it to penetrate the substrate, increasing the probability of physical interactions, and subsequently increasing ... [more ▼]

When repair material is laid down on a concrete substrate, the porosity of concrete allows it to penetrate the substrate, increasing the probability of physical interactions, and subsequently increasing adhesion. Water absorption by immersion or usual capillary action tests do not provide enough information to understand what happens when contact occurs between the concrete substrate and the cement slurries used as a bonding layer. An adaptation of the capillary action test is presented; this is based on a continuous measurement of mass change of the sample. Major differences according to the type of surface preparation are pointed out: absorption rates as well as mass changes are greater for polished concrete substrates than for sandblasted ones. Centrifuged solutions of cement slurries modified with plasticizers and superplasticizers are used for water replacement in the adapted capillary action test. Results clearly show the influence of these admixtures: the greater the adhesion tension, the greater the wetting of the concrete substrate. Determination of these thermodynamic characteristics and performance of an adapted capillary action test provide a better understanding of the mechanisms of interface creation and promotion of adhesion. [less ▲]

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See detailac magnetic behavior of large-grain magnetoresistive La0.78Ca0.22Mn0.90Ox materials
Vanderbemden, Philippe ULg; Vertruyen, Bénédicte ULg; Rulmont, André ULg et al

in Physical Review. B (2003), 68(22), 224418

We report a detailed set of ac magnetic measurements carried out on bulk large grain La-Ca-Mn-O samples extracted from a floating zone method-grown rod. Three samples with La0.78Ca0.22Mn0.90Ox ... [more ▼]

We report a detailed set of ac magnetic measurements carried out on bulk large grain La-Ca-Mn-O samples extracted from a floating zone method-grown rod. Three samples with La0.78Ca0.22Mn0.90Ox stoichiometry but differing in their microstructure were investigated by electrical resistivity and ac susceptibility measurements: (i) a single grain sample, (ii) a sample containing two grains, and (iii) a polycrystalline sample. We show that the superimposition of dc magnetic fields during ac magnetic susceptibility measurements is an efficient way for characterizing the magnetic transition of samples with different microstructures. Whereas both single grain and polycrystalline samples display a single susceptibility peak, an additional kink structure is observed in the case of the double grain sample. The temperature dependence of the ac susceptibility measured with superimposed dc magnetic fields is analyzed in the framework of second-order phase transition ideas. The relations between the critical exponents (beta+gamma ~ 1.5, delta ~ 2.5) are found to be close to those of the mean-field model for all samples. This is attributed to the disordering caused by unoccupied Mn sites. ©2003 The American Physical Society. [less ▲]

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See detailBiosafety of Herpesvirus Vectors
Gogev, S.; Schynts, F.; Meurens, F. et al

in Current Gene Therapy (2003), 3(6), 597-611

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in ... [more ▼]

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in which the viral genome exists as a stable episome in the nucleus. For gene therapy to become a potential future treatment option, biosafe therapeutically efficient gene transfer is a central, but more and more stringent requirement. This review highlights the progress in development of herpesvirus based vectors, describes their properties as wall as discusses the biosafety concerns that are associated with their use in gene therapy. Thought was also given to biosafety issues pertaining to design and production of herpesvirus vector systems in therapeutic gene delivery. [less ▲]

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See detailImpact du Percussionaire® et de la kinésithérapie respiratoire conventionnelle après chirurgie cardiaque : étude randomisée contrôlée
Kellens, Isabelle ULg; Fraipont, V.; Weber, T. et al

in Réanimation (2003, December), 12(suppl 3), 243

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See detailCrural artery bypass with the autogenous greater saphenous vein
Van Damme, Hendrik ULg; Zhang, Lihong ULg; Baguet, E. et al

in European Journal of Vascular and Endovascular Surgery : The Official Journal of the European Society for Vascular Surgery (2003), 26(6), 635-642

Objective. To evaluate the long-term outcome of greater saphenous vein (GSV) infrapopliteal revascularisation in a single centre over a 10 year period. Material and methods. Fourty-one variables relating ... [more ▼]

Objective. To evaluate the long-term outcome of greater saphenous vein (GSV) infrapopliteal revascularisation in a single centre over a 10 year period. Material and methods. Fourty-one variables relating to a consecutive series of 90 crural artery GSV(76% in situ) bypasses in 81 patients (1990-2000) were analysed. The mean age of the 47 men and 34 women was 70 years. Limb-threatening ischaemia was present in 96% of cases, claudication in four patients. In 18 patients, surgery was 'redo'. Results. The perioperative mortality was 3% (n = 3). Patient survival was 54% at 4 years. Independent risk factors affecting survival were chronic renal insufficiency (p = 0.04), hypertension (p = 0.02), and ischaemic heart disease (p = 0.01). Four bypasses thrombosed within 30 days. Three of them could be successfully reopened. Mean follow-up was 39 months. The primary patency rate at 4 years was 80%. Chronic renal insufficiency revealed to be the single independent risk factor for graft thrombosis (p = 0.03, RR = 12.4). The 4-year limb salvage rate was 88%. No independent risk factor affecting the limb salvage could be identified. Conclusion. Crural artery revascularisation is a valuable option for the management of limb threatening infrapopliteal arterial occlusive disease. [less ▲]

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See detailDoes sustained ERP activity in posterior lexico-semantic processing areas during short-term memory tasks only reflect activated long-term memory?
Majerus, Steve ULg; Van der Linden, Martial ULg; Collette, Fabienne ULg et al

in Behavioral and Brain Sciences (2003), 26(6), 746-747

We challenge Ruchkin et al.'s claim in reducing short-term memory (STM) to the active part of long-term memory (LTM), by showing that their data cannot rule out the possibility that activation of ... [more ▼]

We challenge Ruchkin et al.'s claim in reducing short-term memory (STM) to the active part of long-term memory (LTM), by showing that their data cannot rule out the possibility that activation of posterior brain regions could also reflect the contribution of a verbal STM buffer. [less ▲]

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See detailEffects of increased afterload on left ventricular performance and mechanical efficiency are not baroreflex-mediated
Kolh, Philippe ULg; Ghuysen, Alexandre ULg; Tchana-Sato, Vincent ULg et al

in European Journal of Cardio - Thoracic Surgery (2003), 24(6), 912-919

Objective: To assess baroreflex intervention during increase in left ventricular afterload, we compared the effects of aortic banding on the intact cardiovascular system and under hexamethonium infusion ... [more ▼]

Objective: To assess baroreflex intervention during increase in left ventricular afterload, we compared the effects of aortic banding on the intact cardiovascular system and under hexamethonium infusion. Methods: Six open-chest pigs, instrumented for measurement of aortic pressure and flow, left ventricular pressure and volume, were studied under pentobarbital-sufentanil anesthesia. Vascular arterial properties were estimated with a four-element windkessel model. Left ventricular contractility was assessed by the slope of end-systolic pressure-volume relationship. Results: The effects of aortic banding on mechanical aortic properties were unaffected by autonomic nervous system inhibition. However, increase in peripheral arterial vascular resistance and in heart rate were prevented by hexamethonium. Aortic banding increased left ventricular contractility and stroke work. Left ventricular-arterial coupling remained unchanged, but mechanical efficiency was impaired. These ventricular changes were independent of baroreflex integrity. Conclusions: Our results demonstrate that an augmentation in afterload has a composite effect on left ventricular function. Left ventricular performance is increased, as demonstrated by increase in contractility and stroke work, but mechanical efficiency is decreased. These changes are observed independently of baroreflex integrity. Such mechanisms of autoregulation, independent of the autonomic nervous system, are of paramount importance in heart transplant patients. (C) 2003 Elsevier B.V. All fights reserved. [less ▲]

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See detailClinical outcome following combination of cutting balloon angioplasty and coronary β-radiation for in-stent restenosis: A report from the RENO registry
Coucke, Philippe ULg; Roguelov; Eeckhout

in Journal of Invasive Cardiology (The) (2003), 15(12), 706-709

At present, vascular brachytherapy is the only efficient therapy for in-stent restenosis. Nevertheless, edge restenosis often related to geographical miss has been identified as a major limitation of the ... [more ▼]

At present, vascular brachytherapy is the only efficient therapy for in-stent restenosis. Nevertheless, edge restenosis often related to geographical miss has been identified as a major limitation of the technique. The non-slippery cutting balloon has the potential to limit vascular barotraumas, which, together with low-dose irradiation at both ends of the radioactive source, are the prerequisite for geographical miss. This prospective study aimed to examine the efficacy of combining cutting balloon angioplasty and brachytherapy for in-stent restenosis. The Radiation in Europe NOvoste (RENO) registry prospectively tracked all patients who had been treated by coronary β-radiation with the Beta-Cath System (Novoste Corporation, Brussels, Belgium) but were not included in a randomized radiation trial. A subgroup of patients with in-stent restenosis treated by cutting balloon angioplasty and coronary β-radiation (group 1, n = 166) was prospectively defined, and clinical outcomes of patients at 6 months were compared with those of patients treated by conventional angioplasty and coronary β-radiation (group 2, n = 712). At 6-month follow-up, there was a significant difference between groups 1 and 2 in target vessel revascularization (10.2% versus 16.6% respectively; p = 0.04) and in the incidence of major adverse clinical events (MACE) including death, myocardial infarction, and revascularization (10.8% versus 19.2%; p = 0.01). This observation was confirmed by a multivariate analysis indicating a lower risk for MACE at 6 months (odds ratio: 0.49; confidence intervals: 0.27-0.88; p = 0.02). Compared to conventional angioplasty, cutting balloon angioplasty prior to coronary beta-radiation with the Beta-Cath System seems to improve the 6-month clinical outcome in patients with in-stent restenosis. [less ▲]

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See detailEnteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G(2)/M transition
Marches, O.; Ledger, T. N.; Boury, M. et al

in Molecular Microbiology (2003), 50(5), 1553-1567

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement ... [more ▼]

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement' (LEE) to subvert and attach to epithelial cells through the injection of a repertoire of effector molecules. Here, we report the identification of a new TTSS translocated effector molecule called Cif, which blocks cell cycle G(2)/M transition and induces the formation of stress fibres through the recruitment of focal adhesions. Cif is not encoded by the LEE but by a lambdoid prophage present in EPEC and EHEC. A cif mutant causes localized effacement of microvilli and intimately attaches to the host cell surface, but is defective in the ability to block mitosis. When expressed in TTSS competent LEE-positive pathogens, Cif is injected into the infected epithelial cells. These cells arrested at the G(2)/M phase displayed accumulation of inactive phosphorylated Cdk1. In conclusion, Cif is a new member of a growing family of bacterial cyclomodulins that subvert the host eukaryotic cell cycle. [less ▲]

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See detailHumoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Brouta, Frédéric; Descamps, Frédéric; Vermout, Sandy et al

in Medical Mycology (2003), 41(6), 495-501

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a ... [more ▼]

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis. [less ▲]

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See detailQuadro legal da mediaçao vitima-agressor na Europa continental
Lauwaert, Katrien ULg

in Newsletter DGAE (2003), (2), 12-18

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See detailIn vivo dynamic ME-MRI reveals differential functional responses of RA- and area X-projecting neurons in the HVC of canaries exposed to conspecific song
Tindemans, I.; Verhoye, M.; Balthazart, Jacques ULg et al

in European Journal of Neuroscience (2003), 18(12), 3352-3360

HVC (nidopallial area, formerly known as hyperstriatum ventrale pars caudalis), a key centre for song control in oscines, responds in a selective manner to conspecific songs as indicated by ... [more ▼]

HVC (nidopallial area, formerly known as hyperstriatum ventrale pars caudalis), a key centre for song control in oscines, responds in a selective manner to conspecific songs as indicated by electrophysiology. However, immediate-early gene induction cannot be detected in this nucleus following song stimulation. HVC contains neurons projecting either towards the nucleus robustus archistriatalis (RA; motor pathway) or area X (anterior forebrain pathway). Both RA- and area X-projecting cells show auditory responses. The present study analysed these responses separately in the two types of HVC projection neurons of canaries by a new in vivo approach using manganese as a calcium analogue which can be transported anterogradely and used as a paramagnetic contrast agent for magnetic resonance imaging (MRI). Manganese was stereotaxically injected into HVC and taken up by HVC neurons. The anterograde axonal transport of manganese from HVC to RA and area X was then followed by MRI during approximate to 8 h and changes in signal intensity in these targets were fitted to sigmoid functions. Data comparing birds exposed or not to conspecific songs revealed that song stimulation specifically affected the activity of the two types of HVC projection neurons (increase in the sigmoid slope in RA and in its maximum signal intensity in area X). Dynamic manganese-enhanced MRI thus allows assessment of the functional state of specific neuronal populations in the song system of living canaries in a manner reminiscent of functional MRI (but with higher resolution) or of 2-deoxyglucose autoradiography (but in living subjects). [less ▲]

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See detailStabilization of periodic orbits in a wedge billiard
Sepulchre, Rodolphe ULg; Gerard, Manuel

in Proceedings of the 42nd IEEE Conference on Decision and Control (2003, December)

This paper introduces a stabilization problem for an elementary impact control system in the plane. The rich dynamical properties of the wedge billiard, combined to the relevance of the associated ... [more ▼]

This paper introduces a stabilization problem for an elementary impact control system in the plane. The rich dynamical properties of the wedge billiard, combined to the relevance of the associated stabilization problem for feedback control issues in legged robotics make it a valuable benchmark for energy-based stabilization of impact control systems. [less ▲]

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See detailInverse Estimates of Anthropogenic Carbon Dioxide From Ocean Interior Carbon Measurements and Ocean General Circulation Models
Mikaloff Fletcher, S. E.; Gruber, N. P.; Jacobson, A. R. et al

Conference (2003, December)

The ocean is an important sink for atmospheric carbon dioxide, and the exchange of carbon dioxide between the atmosphere and ocean plays a critical role in determining the spatial distribution of ... [more ▼]

The ocean is an important sink for atmospheric carbon dioxide, and the exchange of carbon dioxide between the atmosphere and ocean plays a critical role in determining the spatial distribution of atmospheric carbon dioxide. However, there is still a great deal of uncertainty in both magnitude and regional patterns of anthropogenic uptake associated with estimates of oceanic carbon fluxes. Using a recently developed technique, exchange of anthropogenic carbon dioxide across the air-sea interface have been estimated from observations of dissolved inorganic carbon and nutrient concentrations and an Ocean General Circulation Model (OGCM) using a Green's function inverse modeling technique. Previous sensitivity studies have shown that model circulation error is an important source of error in the ocean inversion. In order to address the role of ocean circulation biases, inverse estimates of anthropogenic carbon air-sea gas exchange are presented using basis functions from a suite of seven different OGCM's. The robustness of the ocean inversion will be quantified and the effects of differences between approaches to modeling ocean circulation on the ocean carbon cycle will be explored. These results will be discussed in the context of recent atmospheric inverse estimates. [less ▲]

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See detailNew disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Le Dall, M. T.; Gaillardin, C. et al

in Journal of Microbiological Methods (2003), 55(3), 727-737

Yarrowia lipolytica is one of the most extensively studied nonconventional yeasts. Unfortunately, few methods for gene disruption have been reported for this yeast, and all of them are time-consuming and ... [more ▼]

Yarrowia lipolytica is one of the most extensively studied nonconventional yeasts. Unfortunately, few methods for gene disruption have been reported for this yeast, and all of them are time-consuming and laborious. The functional analysis of unknown genes requires powerful disruption methods. Here, we describe such a new method for rapid gene disruption in Y lipolytica. This knockout system combines SEP method and the Cre-lox recombination system, facilitating efficient marker rescue. Versatility was increased by using both auxotrophic markers like ylURA3 and ylLEU2, as well as the antibiotic resistance marker hph. The hph marker, which confers resistance to hygromycin-B, allows gene disruption in a strain lacking any conventional auxothrophic marker. The disruption cassette was shown to integrate at the correct locus at an average frequency of 45%. Upon expression of Cre recombinase, the marker was excised at a frequency of 98%, by recombination between the two lox sites. This new method for gene disruption is an ideal tool for the functional analysis of gene families, or for creating large-scale mutant collections in general. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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