References of "2003"
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See detailLes fondations belges d'utilité publique : entre permanence et changements
Pirotte, Gautier ULiege; Heuschen, Laurence

Report (2003)

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See detailErreurs de classification dans les tables de contingence
Magis, David ULiege

Scientific conference (2003, December)

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See detailSite-directed mutagenesis of conserved inverted repeat sequences in the xylanase C promoter region from Streptomyces sp EC3
Giannotta, F.; Georis, J.; Rigali, Sébastien ULiege et al

in Molecular Genetics & Genomics (2003), 270(4), 337-346

Streptomyces sp. EC3, a strain which was originally isolated from cattle manure compost, was shown to possess a strong xylanolytic activity. One of the genes responsible for this activity, xlnC, encodes a ... [more ▼]

Streptomyces sp. EC3, a strain which was originally isolated from cattle manure compost, was shown to possess a strong xylanolytic activity. One of the genes responsible for this activity, xlnC, encodes a secreted xylanase. In the native strain, as in the heterologous host S. lividans, expression of xlnC was detectable in the presence of xylan but not in the presence of glucose. Induction by xylan was shown to take place at the transcriptional level. The transcriptional start site of xlnC was mapped and likely -35 (5'-TTGACA-3') and -10 (5'-GAGAAC-3') motifs were identified. In order to localise putative conserved regulatory sequences, the promoter regions of xylanase-encoding genes from various Streptomyces species were aligned. This alignment revealed the existence of three sets of quite well conserved palindromic AT rich sequences called boxes 1, 2 and 3. Box 3 (5'-CGAAA N TTTCG-3') is the farthest away from the promoter region (150-200 bp). A shorter version of this palindrome (5'-GAAA NN TTTC-3') or (5'-CGAAA-3') constitutes box 1, which is located just upstream of the putative -35 promoter sequence. Box 2, located 5-7 bp upstream of box 1, comprises a shorter palindrome than box 3, with inverted polarity [5'-(G/C)TTTC (N) GAAA(G/C)-3']. The putative regulatory role of the conserved inverted repeats in boxes 2 and 3 in the promoter region of the xlnC gene from Streptomyces sp. EC3, was assessed. These boxes were modified by site-directed mutagenesis, and the mutant promoter regions, as well as the wild-type promoter region, were separately fused to a beta-lactamase reporter gene. Analysis of the expression patterns of these fusions in cultures grown in the presence of glucose, xylan or both carbon sources demonstrated that these motifs were cis -acting negative regulatory elements, each playing a specific role in the regulation of xlnC expression. Box 3 was shown to be critical for the establishment of repression of xlnC expression by glucose, whereas box 2 was shown to play an important role in the induction of xlnC expression by xylan. [less ▲]

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See detailAromatase (estrogen synthase) activity in the dorsal horn of the spinal cord: Functional implications
Evrard, H. C.; Balthazart, Jacques ULiege

in Annals of the New York Academy of Sciences (2003), 1007

The presence of aromatase (estrogen synthase) in neurons in the dorsal horn of the spinal cord in Japanese quail suggests that estrogens produced locally from androgens could control spinal sensory ... [more ▼]

The presence of aromatase (estrogen synthase) in neurons in the dorsal horn of the spinal cord in Japanese quail suggests that estrogens produced locally from androgens could control spinal sensory processes including nociception. We used the hot water nociceptive test (54 degreesC) to appraise the longterm effect of an inhibition of aromatization on the foot withdrawal latency in male quail. Four weeks after the ablation of their main source of testosterone (testes), castrated males displayed a significantly higher foot withdrawal latency than gonadally intact males. A prolonged treatment with subcutaneous capsules filled with testosterone or 17 beta-estradiol restored the baseline latency within 2 weeks. The effect of testosterone in castrated quail was almost completely blocked by systemic injections of Vorozole((TM)), a nonsteroidal aromatase inhibitor or tamoxifen, an estrogen receptor antagonist (one injection per day for 10 days). Taken together, these data demonstrate for the first time to our knowledge an effect of estrogens formed by aromatization of androgens on nociception. Because aromatase-immunoreactive neurons and aromatase activity are present in the dorsal horns of the spinal cord, this control of pain thresholds is presumably mediated, at least in part, by estrogens produced at the spinal level that act locally via slow, presumably genomic, mechanisms mediated by the activation of spinal nuclear estrogen receptors. [less ▲]

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See detailDétermination d'un rendement fromager de laboratoire en relation avec les caractéristiques chimiques de lairs de brebis.
Rondia, P.; Blecker, Christophe ULiege; Romnée, Jean-Michel et al

Poster (2003, December)

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See detailFemmes de science belges : onze vies d'enthousiasme
Faes, Emilie; Jaminon, Martine ULiege

Book published by L'harmattan (2003)

Afin de relever les défis de ce nouveau millénaire, l’Europe a un besoin crucial de scientifiques et de techniciens hautement qualifiés. Pourquoi alors ne pas chercher les compétences requises dans le ... [more ▼]

Afin de relever les défis de ce nouveau millénaire, l’Europe a un besoin crucial de scientifiques et de techniciens hautement qualifiés. Pourquoi alors ne pas chercher les compétences requises dans le réservoir humain gigantesque que les jeunes filles constituent, réservoir trop souvent négligé à ce jour ? Attirer les jeunes filles vers les sciences exactes ou appliquées ? Tout un programme ! Onze récits de vie, onze manifestations d’enthousiasme. Qu’elles soient mathématicienne, géologue, astrophysicienne, physicienne, ingénieure, physicienne, chimiste ou virologue, les sciences comblent les aspirations professionnelles de ces femmes. Mais cet enthousiasme omniprésent, n’occulte-t-il pas les difficultés que les femmes scientifiques rencontrent encore actuellement pour prendre place dans le monde académique, un des derniers bastions de la suprématie masculine ? [less ▲]

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See detail8-Methyl-6-(4-methylpiperazin-1-yl)-11H-pyrido[2,3-b][1,4]benzodiazepine
Spirlet, Marie-Rose ULiege; Graulich, Amaury ULiege; Liégeois, Jean-François ULiege

in Acta Crystallographica Section E-Structure Reports Online (2003), 59(Part 12), 1990-1991

The conformation of the title compound, C18H21N5, is very similar to that observed in other diaryldiazepine structures such as clozapine and clozapine dihydrobromide. N-H...H hydrogen-bond interactions ... [more ▼]

The conformation of the title compound, C18H21N5, is very similar to that observed in other diaryldiazepine structures such as clozapine and clozapine dihydrobromide. N-H...H hydrogen-bond interactions result in the formation of a dimer. [less ▲]

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See detailL'image du mois. Myxome de l'oreillette gauche
Tombeux, Christophe ULiege; Davin, Laurent ULiege; PIERARD, Luc ULiege et al

in Revue Médicale de Liège (2003), 58(12), 723

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See detailPrix de référence pour les honoraires médico-techniques dans les hôpitaux
Gillet, Pierre ULiege

Diverse speeche and writing (2003)

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See detailExperimental study of the thermo-hydraulic performances of humid air condensers
Cuevas, Cristian; Trebilcock, Felipe; Ngendakumana, Philippe ULiege

Report (2003)

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See detail8-Chloro-5-(4-methylpiperazin-1-yl)-11H-pyrido[2,3-b][1,5]benzoxazepine
Dupont, Léon ULiege; Liégeois, Jean-François ULiege

in Acta Crystallographica Section E-Structure Reports Online (2003), 59(Part 12), 1962-1963

The crystal structure of the title compound, C17H17ClN4O, has been undertaken as part of our studies of dopamine receptors. The oxazepine ring has a boat conformation, while the piperazine ring is in a ... [more ▼]

The crystal structure of the title compound, C17H17ClN4O, has been undertaken as part of our studies of dopamine receptors. The oxazepine ring has a boat conformation, while the piperazine ring is in a normal chair conformation. The dihedral angle between the two aromatic rings that lie on the same side of the oxazepine moiety is 113.99 (7)degrees. There is no hydrogen bonding. [less ▲]

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See detailA capillary action test for the investigation of adhesion in repair technology
Courard, Luc ULiege; Degeimbre, Robert ULiege

in Canadian Journal of Civil Engineering (2003), 30(6), 1101-1110

When repair material is laid down on a concrete substrate, the porosity of concrete allows it to penetrate the substrate, increasing the probability of physical interactions, and subsequently increasing ... [more ▼]

When repair material is laid down on a concrete substrate, the porosity of concrete allows it to penetrate the substrate, increasing the probability of physical interactions, and subsequently increasing adhesion. Water absorption by immersion or usual capillary action tests do not provide enough information to understand what happens when contact occurs between the concrete substrate and the cement slurries used as a bonding layer. An adaptation of the capillary action test is presented; this is based on a continuous measurement of mass change of the sample. Major differences according to the type of surface preparation are pointed out: absorption rates as well as mass changes are greater for polished concrete substrates than for sandblasted ones. Centrifuged solutions of cement slurries modified with plasticizers and superplasticizers are used for water replacement in the adapted capillary action test. Results clearly show the influence of these admixtures: the greater the adhesion tension, the greater the wetting of the concrete substrate. Determination of these thermodynamic characteristics and performance of an adapted capillary action test provide a better understanding of the mechanisms of interface creation and promotion of adhesion. [less ▲]

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See detailBiosafety of Herpesvirus Vectors
Gogev, S.; Schynts, F.; Meurens, F. et al

in Current Gene Therapy (2003), 3(6), 597-611

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in ... [more ▼]

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections in which the viral genome exists as a stable episome in the nucleus. For gene therapy to become a potential future treatment option, biosafe therapeutically efficient gene transfer is a central, but more and more stringent requirement. This review highlights the progress in development of herpesvirus based vectors, describes their properties as wall as discusses the biosafety concerns that are associated with their use in gene therapy. Thought was also given to biosafety issues pertaining to design and production of herpesvirus vector systems in therapeutic gene delivery. [less ▲]

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See detailDoes sustained ERP activity in posterior lexico-semantic processing areas during short-term memory tasks only reflect activated long-term memory?
Majerus, Steve ULiege; Van der Linden, Martial ULiege; Collette, Fabienne ULiege et al

in Behavioral and Brain Sciences (2003), 26(6), 746-747

We challenge Ruchkin et al.'s claim in reducing short-term memory (STM) to the active part of long-term memory (LTM), by showing that their data cannot rule out the possibility that activation of ... [more ▼]

We challenge Ruchkin et al.'s claim in reducing short-term memory (STM) to the active part of long-term memory (LTM), by showing that their data cannot rule out the possibility that activation of posterior brain regions could also reflect the contribution of a verbal STM buffer. [less ▲]

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See detailEffects of increased afterload on left ventricular performance and mechanical efficiency are not baroreflex-mediated
Kolh, Philippe ULiege; Ghuysen, Alexandre ULiege; Tchana-Sato, Vincent ULiege et al

in European Journal of Cardio - Thoracic Surgery (2003), 24(6), 912-919

Objective: To assess baroreflex intervention during increase in left ventricular afterload, we compared the effects of aortic banding on the intact cardiovascular system and under hexamethonium infusion ... [more ▼]

Objective: To assess baroreflex intervention during increase in left ventricular afterload, we compared the effects of aortic banding on the intact cardiovascular system and under hexamethonium infusion. Methods: Six open-chest pigs, instrumented for measurement of aortic pressure and flow, left ventricular pressure and volume, were studied under pentobarbital-sufentanil anesthesia. Vascular arterial properties were estimated with a four-element windkessel model. Left ventricular contractility was assessed by the slope of end-systolic pressure-volume relationship. Results: The effects of aortic banding on mechanical aortic properties were unaffected by autonomic nervous system inhibition. However, increase in peripheral arterial vascular resistance and in heart rate were prevented by hexamethonium. Aortic banding increased left ventricular contractility and stroke work. Left ventricular-arterial coupling remained unchanged, but mechanical efficiency was impaired. These ventricular changes were independent of baroreflex integrity. Conclusions: Our results demonstrate that an augmentation in afterload has a composite effect on left ventricular function. Left ventricular performance is increased, as demonstrated by increase in contractility and stroke work, but mechanical efficiency is decreased. These changes are observed independently of baroreflex integrity. Such mechanisms of autoregulation, independent of the autonomic nervous system, are of paramount importance in heart transplant patients. (C) 2003 Elsevier B.V. All fights reserved. [less ▲]

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See detailEnteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G(2)/M transition
Marches, O.; Ledger, T. N.; Boury, M. et al

in Molecular Microbiology (2003), 50(5), 1553-1567

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement ... [more ▼]

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement' (LEE) to subvert and attach to epithelial cells through the injection of a repertoire of effector molecules. Here, we report the identification of a new TTSS translocated effector molecule called Cif, which blocks cell cycle G(2)/M transition and induces the formation of stress fibres through the recruitment of focal adhesions. Cif is not encoded by the LEE but by a lambdoid prophage present in EPEC and EHEC. A cif mutant causes localized effacement of microvilli and intimately attaches to the host cell surface, but is defective in the ability to block mitosis. When expressed in TTSS competent LEE-positive pathogens, Cif is injected into the infected epithelial cells. These cells arrested at the G(2)/M phase displayed accumulation of inactive phosphorylated Cdk1. In conclusion, Cif is a new member of a growing family of bacterial cyclomodulins that subvert the host eukaryotic cell cycle. [less ▲]

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See detailHumoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Brouta, Frédéric; Descamps, Frédéric; Vermout, Sandy et al

in Medical Mycology (2003), 41(6), 495-501

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a ... [more ▼]

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis. [less ▲]

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See detailIn vivo dynamic ME-MRI reveals differential functional responses of RA- and area X-projecting neurons in the HVC of canaries exposed to conspecific song
Tindemans, I.; Verhoye, M.; Balthazart, Jacques ULiege et al

in European Journal of Neuroscience (2003), 18(12), 3352-3360

HVC (nidopallial area, formerly known as hyperstriatum ventrale pars caudalis), a key centre for song control in oscines, responds in a selective manner to conspecific songs as indicated by ... [more ▼]

HVC (nidopallial area, formerly known as hyperstriatum ventrale pars caudalis), a key centre for song control in oscines, responds in a selective manner to conspecific songs as indicated by electrophysiology. However, immediate-early gene induction cannot be detected in this nucleus following song stimulation. HVC contains neurons projecting either towards the nucleus robustus archistriatalis (RA; motor pathway) or area X (anterior forebrain pathway). Both RA- and area X-projecting cells show auditory responses. The present study analysed these responses separately in the two types of HVC projection neurons of canaries by a new in vivo approach using manganese as a calcium analogue which can be transported anterogradely and used as a paramagnetic contrast agent for magnetic resonance imaging (MRI). Manganese was stereotaxically injected into HVC and taken up by HVC neurons. The anterograde axonal transport of manganese from HVC to RA and area X was then followed by MRI during approximate to 8 h and changes in signal intensity in these targets were fitted to sigmoid functions. Data comparing birds exposed or not to conspecific songs revealed that song stimulation specifically affected the activity of the two types of HVC projection neurons (increase in the sigmoid slope in RA and in its maximum signal intensity in area X). Dynamic manganese-enhanced MRI thus allows assessment of the functional state of specific neuronal populations in the song system of living canaries in a manner reminiscent of functional MRI (but with higher resolution) or of 2-deoxyglucose autoradiography (but in living subjects). [less ▲]

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See detailLa médecine dans l'Empire romain au premier siècle de notre ère
Marganne, Marie-Hélène ULiege

in Biblia (2003), 24

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