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See detailIdentificación y caracterización de un nuevo elemento regulatorio que involucra al factor transcripcional E2F4 en la región U5 del LTR del virus de la leucosis bovina
Rodriguez, Sabrina ULg; Varone, C.; Cánepa, E. et al

Poster (2003, December 01)

IDENTIFICACIÓN Y CARACTERIZACIÓN DE UN NUEVO ELEMENTO REGULATORIO QUE INVOLUCRA AL FACTOR TRANSCRIPCIONAL E2F-4 EN LA REGIÓN U5 DEL LTR DEL VIRUS DE LA LEUCOSIS BOVINA S. Rodriguez1, C. Varone2, E ... [more ▼]

IDENTIFICACIÓN Y CARACTERIZACIÓN DE UN NUEVO ELEMENTO REGULATORIO QUE INVOLUCRA AL FACTOR TRANSCRIPCIONAL E2F-4 EN LA REGIÓN U5 DEL LTR DEL VIRUS DE LA LEUCOSIS BOVINA S. Rodriguez1, C. Varone2, E. Cánepa2, K. Trono1 1Instituto de Virología, CICVyA. INTA – Castelar. 2Cátedra de Biologia Molecular, Facultad de Ciencias Exactas, UBA. Buenos Aires, Argentina. El genoma del virus de la leucosis bovina (VLB), como todo retrovirus, presenta en ambos extremos zonas denominadas Long Terminal Repeats (LTRs) de importancia en la regulación de la transcripción. Estas regiones están organizadas en tres zonas llamadas U3, R y U5. La región U3 ha sido ampliamente estudiada y se sabe que contiene los principales sitios que regulan la transcripción viral. Sin embargo, poco se sabe de las regiones R y U5 aunque ha quedado demostrado que ambas zonas contienen asimismo secuencias estimulatorias. Con el objetivo de identificar y caracterizar nuevos elementos regulatorios, en este trabajo se ha llevado a cabo el estudio de secuencias de las regiones R y U5 del LTR 5´ de BLV. El análisis de patrones de reconocimiento de secuencias consenso para factores de transcripción celulares en estas regiones, reveló la presencia de 2 posibles elementos de unión para E2F, ambos en la zona U5, denominados E2F-A y E2F-B, respectivamente. Para analizar la capacidad de unión a factores celulares de las secuencias LTRs se llevaron a cabo ensayos de cambio en la movilidad electroforética (EMSA) y supershift utilizando extractos celulares obtenidos a partir de linfocitos bovinos y empleando los oligonucleótidos U51 y U52 como sondas, donde mapean los elementos E2FA y E2FB, respectivamente. Se observó retardo por la formación de complejos ribo-proteicos en ambos casos pero solo en el caso del complejo U51-E2F pudo ser identificado el factor E2F-4 utilizando anticuerpos específicos. En resumen, estos resultados permiten identificar un nuevo sitio para el factor de transcripción E2F-4 localizado en la región U5 del LTR 5´ de BLV. Este motivo constituye un nuevo elemento regulatorio involucrado en la actividad transcripcional de genes virales y celulares dirigida por los LTRs y podría jugar un rol importante en la replicación viral. [less ▲]

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See detailDistintas variantes naturales del BLV presentan patrones diferenciales de unión a factores celulares
Rodriguez, Sabrina ULg; Varone, C.; Mozgovoj, M. et al

Poster (2003, December 01)

DISTINTAS VARIANTES NATURALES DEL BLV PRESENTAN PATRONES DIFERENCIALES DE UNIÓN A FACTORES CELULARES. S. Rodriguez1, C. Varone2, M. Mozgovoj1, C. Gómez1, M.J. DusSantos1, A. Wigdorovitz1, E. Cánepa2, K ... [more ▼]

DISTINTAS VARIANTES NATURALES DEL BLV PRESENTAN PATRONES DIFERENCIALES DE UNIÓN A FACTORES CELULARES. S. Rodriguez1, C. Varone2, M. Mozgovoj1, C. Gómez1, M.J. DusSantos1, A. Wigdorovitz1, E. Cánepa2, K. Trono1 1Instituto de Virología, CICVyA, INTA – Castelar. 2Cátedra de Biologia Molecular, Facultad de Ciencias Exactas, UBA. Buenos Aires, Argentina. La relación entre el genotipo y las formas de manifestación patogénica está demostrada para ciertos Retrovirus, y las zonas regulatorias Long Terminal Repeats (LTR) han sido descriptas, entre otros factores, como portadoras de determinantes de patogenia. En el caso del Virus de la Leucosis Bovina (VLB), estudios previos de secuencias llevados a cabo en nuestro laboratorio sobre las zona R-U5 de las regiones genéticas provirales LTR aisladas de 36 animales infectados naturalmente y con diferentes formas de evolución revelaron la presencia de 4 cambios fijos y característicos de provirus aislados de linfosarcoma (LS)(100%), 1 en la región R y 3 en U5. Estas modificaciones están ausentes en la mayoría (16/22) de los provirus aislados de formas asintomáticas (AL/PL). La alta asociación entre los cambios de nucleótidos y las formas de patogenia demostró la existencia de una secuencia de nucleótidos consenso y propia de los aislados de LS en la región U5 del LTR del genoma proviral de VLB. Con el objetivo de analizar si existe una capacidad diferencial de reconocimiento de factores de regulación de la transcripción celular en la célula hospedadora de las secuencias LTRs de aislamientos de distintas formas de patogenia, se utilizaron ensayos de cambio en la movilidad electroforética (EMSA). La evaluación conjunta de los resultados demuestra que existirían diferencias específicas de unión de la región U5 a proteínas del complejo de regulación de la transcripción celular denominadas E2F en relación con las diferentes presentaciones de esta patología. Estos hallazgos aportan datos en relación con la caracterización de esta co-variación de nucleótidos como marcadores de patogenia del VLB, así como también representan un adelanto en el conocimiento de la patobiología del BLV. [less ▲]

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See detailExecutive dysfunction in dementia
Collette, Fabienne ULg

Conference (2003, December)

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See detailEndometriose stade I et II: implications physiopathologiques, cliniques et therapeutiques
Nisolle, Michelle ULg; Nervo, Patricia ULg

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2003), 32(8, Pt 2), 11-4

Stage I and II endometriosis is defined by a r-AFS score respectively ranging from 1 to 5 and from 6 to 15. This mild, superficial endometriosis is a very common pathology occurring in infertile women ... [more ▼]

Stage I and II endometriosis is defined by a r-AFS score respectively ranging from 1 to 5 and from 6 to 15. This mild, superficial endometriosis is a very common pathology occurring in infertile women. Nevertheless, these women with stage I/II endometriosis have usually few pelvic pain. This review summarizes the recent literature concerning new data on the pathogenesis of peritoneal endometriosis and its clinical management. Retrograde menstruation, peritoneal adhesion of shed endometrial tissue, and outgrowth of endometrial cells, glands and stroma, are essential elements in the pathogenesis of endometriosis according to Sampson's classic implantation theory. Nevertheless, exact pathophysiology of endometriosis remains unknown. Superficial endometriotic lesions observed by laparoscopy have to be treated. Surgical procedure is not difficult for stage I and II of endometriosis. Surgical procedure remains controversial. Carbon dioxide (CO2) Laser can be used for laparoscopic destruction of endometriosis. Newer procedures, such as SurgiTouch (Lumenis), are more effective in vaporization and decrease the risk thermal damage of contiguous structures. The monopolar scissors can also be used in order to excise the peritoneal endometriotic lesions. Medical treatment may be usefull if surgical treatment is not complete or if the pelvic cavity is hypervascularized. In these cases, Gonadotropin-Releasing Hormone agonists (Gn-RHa) are the most common and effective treatment. [less ▲]

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See detailLCA et rééducation isocinétique : analyse critique
Croisier, Jean-Louis ULg

in Actes de la XXIIème Journée scientifique de l’AMISEK « Controverses en chirurgie et rééducation du genou » (2003, December)

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See detailDétermination d'un rendement fromager de laboratoire en relation avec les caractéristiques chimiques de lairs de brebis.
Rondia, P.; Blecker, Christophe ULg; Romnée, Jean-Michel et al

Poster (2003, December)

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See detailCerebellar metabolite alterations detected in vivo by proton MR spectroscopy
Macri, M. A.; Garreffa, G.; Giove, F. et al

in Magnetic Resonance Imaging (2003), 21(10), 1201-1206

The aim of our work was to evaluate the feasibility of in vivo single-voxel quantitative proton MR spectroscopy in order to identify possible alterations in the main metabolite concentrations due to some ... [more ▼]

The aim of our work was to evaluate the feasibility of in vivo single-voxel quantitative proton MR spectroscopy in order to identify possible alterations in the main metabolite concentrations due to some metabolic dysfunctions in the cerebellum of patients suffering from a particular form of migraine called "with aura." Measurements of metabolite levels in the cerebellum disclosed reduced choline values (normalized both to N-acetyl-aspartate and creatine) in the patient group with respect to the age-matched control group. Our interest in this pathology is motivated by the fact that there are no available specific biochemical markers for migraine characterization, and the current diagnostic only takes advantage of the medical history and the clinical examination. (C) 2003 Elsevier Inc. All rights reserved. [less ▲]

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See detailSoft tissue attachment formation at titanium abutments in humans: a new model
Rompen, Eric ULg; Domken, Olivier ULg; Lamy, Marc ULg et al

in Journal of Dental Research (2003, December), 82(Sp. Iss. C), 510

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See detailComparative efficacy of eletriptan and zolmitriptan in the acute treatment of migraine
Steiner, T. J.; Diener, H. C.; MacGregor, E. A. et al

in Cephalalgia : An International Journal of Headache (2003), 23(10), 942-952

Eletriptan 40 mg and 80 mg have shown greater efficacy in acute migraine than oral sumtriptan 100 mg and naratriptan 2.5 mg. This study continues the systematic series of active comparator trials in the ... [more ▼]

Eletriptan 40 mg and 80 mg have shown greater efficacy in acute migraine than oral sumtriptan 100 mg and naratriptan 2.5 mg. This study continues the systematic series of active comparator trials in the eletriptan clinical development programme. In a multicentre double-blind, double-dummy, parallel-groups trial, 1587 outpatients with migraine by IHS criteria were randomised in a 3: 3: 3: 1 ratio to eletriptan 80 mg, eletriptan 40 mg, zolmitriptan 2.5 mg or placebo. Of these, 1312 treated a single migraine attack and recorded baseline and outcome data to be included in the intention-to-treat population. The primary analysis was between eletriptan 80 mg and zolmitriptan. For the primary efficacy end-point of 2-h headache response, rates were 74% on eletriptan 80 mg, 64% on eletriptan 40 mg, 60% on zolmitriptan (P < 0.0001 vs. eletriptan 80 mg) and 22% on placebo (P < 0.0001 vs. all active treatments). Eletriptan 80 mg was superior to zolmitriptan on all secondary end-points at 1, 2 and 24 h, in most cases with statistical significance. Eletriptan 40 mg had similar efficacy to zolmitriptan 2.5 mg in earlier end-points, and significantly (P < 0.05) lower recurrence rate and need for rescue medication over 24 h. All treatments were well tolerated; 30-42% of patients on active treatments and 40% on placebo reported all-causality adverse events that were mostly mild and transient. On patients' global ratings of treatment, both eletriptan doses scored significantly better than zolmitriptan. [less ▲]

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See detailPreventive assessment and enhancement of power system voltage stability: an integrated approach of thermal and voltage security
Capitanescu, Florin ULg

Doctoral thesis (2003)

This thesis is devoted to the preventive assessment and enhancement of voltage stability and security in electric power systems. However, in the course of deriving all the proposed methods we have paid ... [more ▼]

This thesis is devoted to the preventive assessment and enhancement of voltage stability and security in electric power systems. However, in the course of deriving all the proposed methods we have paid attention to keeping them compatible with the (more traditional) handling of thermal overloads, thereby providing a unified treatment of voltage and thermal security. The approaches presented in this work apply to both deregulated environments and classical, vertically integrated ones. The heart of most methods developed in this thesis is : (i) the derivation of sensitivities indicating the relative efficiency of the various bus injections to restore voltage stability or increase an insufficient voltage security margin, and (ii) the use of these sensitivities in linearized security constraints that can be incorporated to various optimization problems. Using this formulation, we deal with three different problems of interest in preventive security analysis: 1) Congestion management. We propose two optimization-based approaches to manage congestions due to voltage instability and/or thermal overload. The control variables are either power injections (generation rescheduling and load curtailment) or power transactions; 2) Computation of Available Transfer Capabilities (ATCs). We determine the simultaneous ATCs of multiple transactions by means of a single optimization-based computation; 3) evaluation of security margins interval. To face the uncertainty affecting power transfers, we present an optimization-based computation of the minimal and maximal margins under the assumption that individual injections vary within specified bounds. Besides this main theme, the thesis offers additional reflections on the: 1) Filtering of contingencies. We propose a simple and reliable technique to filter out harmless contingencies when computing voltage security margins of a large set of contingencies; 2) Evaluation of reactive reserves with respect to a contingency, an important topic for voltage security reasons as well as within the context of a deregulated market where providing reactive reserves is an ancillary service which should be properly paid. Most of the methods proposed in this thesis were successfully tested on realistic power system models. From a practical viewpoint all the above computations have been coupled to the fast time-domain quasi steady-state simulation used in the ASTRE software developed at the University of Liège. [less ▲]

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See detailMale hypogonadism caused by isolated LH deficiency : From pathology to gene, from gene to physiology
Valdes Socin, Hernan Gonzalo ULg; Salvi, R.; Pralong, F. et al

in Meeting of the Belgian Endocrine Society : December 2003 (2003, December)

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See detailLe casier judiciaire central
Derre, Sylvie; Seron, Vincent ULg

in Jacobs, Ann; Masset, Adrien (Eds.) Actualités de droit pénal et de procédure pénale (2003)

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See detailSegmentation by adaptive prediction and region merging
Van Droogenbroeck, Marc ULg; Talbot, Hugues

in Digital Image Computing Techniques and Applications, Volume II (2003, December)

This paper presents a segmentation technique based on prediction and adaptive region merging. While many techniques for segmentation exist, few of them are suited for the segmentation of natural images ... [more ▼]

This paper presents a segmentation technique based on prediction and adaptive region merging. While many techniques for segmentation exist, few of them are suited for the segmentation of natural images containing regular textures defined on non-rectangular segments. In this paper, we propose a description of regions based on a deconvolution algorithm whose purpose is to remove the influence of the shape on region contents. The decoupling of shape and texture information is achieved either by adapting waveforms to the segment shape, which is a time-consuming task that needs to be repeated for each segment shape, or by the extrapolation of a signal to fit a rectangular window, which is the chosen path. The deconvolution algorithm is the key of a new segmentation technique that uses extrapolation as a prediction of neighbouring regions. When the prediction of a region fits the actual content of a connected region reasonably well, both regions are merged. The segmentation process starts with an over-segmented image. It progressively merges neighbouring regions whose extrapolations fit according to an energy criterion. After each merge, the algorithm updates the values of the merging criterion for regions connected to the merged region pair. It stops when no further gain is achieved in merging regions or when mean values of adjacent regions are too different. Simulation results indicate that, although our technique is tailored for natural images containing periodic signals and flat regions, it is in fact usable for a large set of natural images. [less ▲]

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See detailEffect of hemodiafiltration on pulmonary hemodynamics in endotoxic shock
Lambermont, Bernard ULg; Kolh, Philippe ULg; Ghuysen, Alexandre ULg et al

in Artificial Organs (2003), 27(12), 1128-1133

Hemofiltration can improve pulmonary hemodynamics during septic shock. The main objective of the study was to determine whether hemodiafiltration (HDF) would also have beneficial effects on pulmonary ... [more ▼]

Hemofiltration can improve pulmonary hemodynamics during septic shock. The main objective of the study was to determine whether hemodiafiltration (HDF) would also have beneficial effects on pulmonary hemodynamics during septic shock. In the Endo group, six anesthetized pigs received a 0.5 mg/kg endotoxin infusion over 30 min. In the HDF group (n = 6), HDF was started 30 min after the end of the endotoxin infusion, while in the Control group (n = 4) they received HDF but no endotoxin infusion. Pulmonary hemodynamics were analyzed in detail with a four-element windkessel model. Although in the Control group, HDF did not alter pulmonary hemodynamic parameters, in the HDF group, it was responsible for an amplification of the deleterious pulmonary vascular response to endotoxin insult. Our results show that HDF must be used cautiously in septic shock since it can precipitate right heart failure by increasing pulmonary vascular resistance. [less ▲]

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