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See detailLarval development of Myzostoma cirriferum (Myzostomida)
Eeckhaut, I.; Fievez, Laurence ULg; Muller, M. C.

in Journal of Morphology (2003), 258

The larval development of Myzostoma cirriferum is described by means of SEM, TEM, and cLSM. It is similar to that of other myzostomids and includes three stages: the protrochophore, the trochophore, and ... [more ▼]

The larval development of Myzostoma cirriferum is described by means of SEM, TEM, and cLSM. It is similar to that of other myzostomids and includes three stages: the protrochophore, the trochophore, and the metatrochophore. The protrochophore is a ball-shaped larva present in culture from 18-48 h after egg laying. It has no internal organs and its body is made of three cell types: covering cells and ciliated cells that are external and surrounded by a cuticle, and resting cells that fill the blastocoel. The trochophore is a pear-shaped larva that develops 20-72 h after egg laying; the body includes the same three cell types as the previous stage. The metatrochophore is a pear-shaped larva that develops between 40 h and 14 days and is characterized by the presence of two bundles of four chaetae. When fully developed, the metatrochophore has a digestive system (made of a pharynx, an esophagus, and a blind digestive pouch), two pairs of protonephridia, and a nervous system composed of a supraesophageal ganglion, circumesophageal connectives, and dorsal and ventral nerves. Metamorphosis generally occurs 7 days after egg laying. At that time, the metatrochophore loses its chaetae and becomes pleated ventrally. This ultrastructural analysis suggests that chaetae and the five ventral longitudinal nerve cords of M. cirriferum metatrochophores are homologous structures to those observed in some polychaete trochophores. Coupled with recent phylogenetic analyses, where the Myzostomida are placed outside the Annelida, homologies between myzostomid and polychaete larvae support the view that a trochophore appeared early during the spiralian evolution [less ▲]

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See detailCatabolism of hydroxyacids and biotechnological production of lactones by Yarrowia lipolytica
Waché, Y.; Aguedo, Mario ULg; Nicaud, J.-M. et al

in Applied Microbiology and Biotechnology (2003), 61(5-6), 393-404

The γ- and δ-lactones of less than 12 carbons constitute a group of compounds of great interest to the flavour industry. It is possible to produce some of these lactones through biotechnology. For ... [more ▼]

The γ- and δ-lactones of less than 12 carbons constitute a group of compounds of great interest to the flavour industry. It is possible to produce some of these lactones through biotechnology. For instance, γ-decalactone can be obtained by biotransformation of methyl ricinoleate. Among the organisms used for this bioproduction, Yarrowia lipolytica is a yeast of choice. It is well adapted to growth on hydrophobic substrates, thanks to its efficient and numerous lipases, cytochrome P450, acylCoA oxidases and its ability to produce biosurfactants. Furthermore, genetic tools have been developed for its study. This review deals with the production of lactones by Y. lipolytica with special emphasis on the biotransformarion of methyl ricinoleate to γ-decalactone. When appropriate, information from the lipid metabolism of other yeast species is presented. [less ▲]

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See detailCassini UVIS time-resolved Jupiter auroral data compared to QP radio bursts
Pryor, W.; Hospodarsky, G.; Stewart, I. et al

Poster (2003)

The Cassini Ultraviolet Imaging Spectrograph (UVIS) observed Jupiter in a 4-sec integration "high time-resolution mode" on Jan 8, 13-14, and 20-21 in 2001. In this mode Extreme-Ultraviolet and Far ... [more ▼]

The Cassini Ultraviolet Imaging Spectrograph (UVIS) observed Jupiter in a 4-sec integration "high time-resolution mode" on Jan 8, 13-14, and 20-21 in 2001. In this mode Extreme-Ultraviolet and Far-Ultraviolet spectra were obtained with reduced spectral and spatial resolution in order to study rapid variations in H2 band and H Lyman alpha emission. Previous work has shown that the region inside Jupiter's main auroral ovals contains highly variable spots of emission (auroral flares) that persist for typically 1 or 2 minutes. This duration is similar to that in Jupiter's quasi-periodic (QP) radio bursts. We compare UVIS data to simultaneous Galileo Plasma Wave Subsystem (PWS) and Cassini Radio PWS (RPWS) observations. Jan 8 was an active period for UV variability, that we associate with polar auroral flares. There is a correlation between the radio and UV bursts in this period, suggesting that they are related phenomena. We will also explore coordinated Hubble Space Telescope Imaging Spectrograph time-tagged UV images from Jan 13-14 and Jan 20-21, 2001 to study the spatial properties of the auroral flares. [less ▲]

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See detailCompte-rendu de l'ouvrage de I. Loudon (ed.) : Western Medicine. An Illustrated History
Nissen, Cécile ULg

in Archives Internationales d'Histoire des Sciences (2003)

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See detailTrou Al'Wesse : Rapport préliminaire des fouilles 2003
Miller, Rebecca ULg; Otte, Marcel ULg

in Notae Praehistoricae (2003), 23

This article presents a summary of the first field season of the second phase of excavations at the site of Trou Al'Wesse as well as proposing a series of hypotheses concerning possible contacts between ... [more ▼]

This article presents a summary of the first field season of the second phase of excavations at the site of Trou Al'Wesse as well as proposing a series of hypotheses concerning possible contacts between Mesolithic and Neolithics populations. [less ▲]

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See detailUse of Hsf1(-/-) mice reveals an essential role for HSF1 to protect lung against cadmium-induced injury
Wirth, D.; Christians, E.; Li, Xiang ULg et al

in Toxicology and Applied Pharmacology (2003), 192(1), 12-20

Cadmium (Cd) is known to activate heat shock (HS) response, which is characterized by overexpression of heat shock proteins (Hsps) under the control of heat shock factor 1 (HSF1). The potential protection ... [more ▼]

Cadmium (Cd) is known to activate heat shock (HS) response, which is characterized by overexpression of heat shock proteins (Hsps) under the control of heat shock factor 1 (HSF1). The potential protection provided by the HS response, induced by increasing the body temperature of animals before Cd exposure or by Cd itself, against pathophysiological changes occurring after Cd intranasal instillation (1 to 100 microg/mouse) was examined. HSF1-deficient mice were used to evaluate the role of this factor in lung protection. Cd instillation caused dose- and time-dependent changes in the respiratory pattern measured by plethysmography (Penh), and significant increases in lactate dehydrogenase (LDH) activity as well as macrophage and neutrophil counts in bronchoalveolar lavage fluids. HS preconditioning induced Hsp overexpression and reduced the Penh (-30%), LDH (-25%), and neutrophil (-55%) responses to subsequent administration of the highest Cd doses (50 and 100 microg) in wild-type mice. HSF1 deficiency abolished the HS response and its protective effect. In the absence of preconditioning, Hsf1(-/-) mice exhibited higher values of Penh (+70%) and LDH activity (+42%) compared with wild-type animals when exposed to the lowest Cd doses. Higher macrophage (+80%) and neutrophil counts (+115%) were recorded whatever the dose. Western blot analyses indicated that lung protection might be related to the kinetics of HSF1-dependent Hsp70 expression. Altogether, our data demonstrate that HS response elicited both by prior HS and by Cd itself moderates pulmonary injuries due to Cd instillation, and that HSF1 is a major mediator in this protection. [less ▲]

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See detailOnychomycosis under the microscope
PIERARD, Gérald ULg; ARRESE ESTRADA, Jorge ULg; PIERARD-FRANCHIMONT, Claudine ULg et al

Book published by Janssen Pharmaceutica (2003)

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See detailLes délinquants sexuels : théories, évaluation et traitements
Cornet, Jean-Philippe; Giovannangeli, Daniel ULg; Mormont, Christian ULg

Book published by Frison-Roche (2003)

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See detailContrats par voie électronique et protection des consommateurs
Biquet, Christine ULg; Decharneux, Joëlle ULg

in Biquet, Christine (Ed.) Contrats à distance et protection des consommateurs (2003)

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See detailFlow cytometric cerebrospinal fluid analysis in children
Häusler, M.; Sellhaus, B.; Schweizer, K. et al

in Pathology Research and Practice (2003), 199(10), 667-675

Flow cytometry (FC) is of increasing importance for the analysis of cerebrospinal fluid (CSF) lymphocytes because of its ability to detect a large spectrum of cellular characteristics (granularity, volume ... [more ▼]

Flow cytometry (FC) is of increasing importance for the analysis of cerebrospinal fluid (CSF) lymphocytes because of its ability to detect a large spectrum of cellular characteristics (granularity, volume, surface antigen expression) even in small amounts of cells. Data on CSF FC in children are very limited. Here, we summarize our 3-year experience of CSF FC routinely performed in pediatric patients with assumed inflammatory central nervous system (CNS) disease. Among 109 samples sent for analysis, flow cytometric detection of major leukocyte subsets was possible in 78% (85 out of 109), which exceeds the 31% rate of our retrospective microscopic pediatric control group. Apart from physiologic lymphocytes (100%) or monocytes (48%), 11 out of these 85 samples showed granulocytes, two showed proliferated monocytes, and nine displayed proliferated lymphocytes. In most children, the proliferated lymphocytes consisted of a polyclonal population of CD4+ and CD8+ T cells. Compared with literature data, eight children showed abnormally composed lymphocyte subsets (surface antigen expression) within the main lymphocyte population. However, none of these changes was specific for distinct diseases or allowed a distinction between patients with and without primary inflammatory processes. These data suggest that CSF FC may be the most effective modality to differentiate major CSF leukocyte subsets. At present, further differentiation of distinct cell populations, such as proliferated lymphocytes, is of limited clinical impact. This may, however, gain increasing interest in the future. [less ▲]

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See detailFacteurs de virulence et propriétés spécifiques des souches invasives d'Escherichia coli : I) les adhésines et facteurs de colonisation
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2003), 147(2, APR-MAY), 105-126

Escherichia coli bacterial species is subdivided into several strains that are pathogenic for man and animals, on the basis of their specific properties and factors which are responsible for their ... [more ▼]

Escherichia coli bacterial species is subdivided into several strains that are pathogenic for man and animals, on the basis of their specific properties and factors which are responsible for their pathogenic characters. The pathogenic strains are classically subdivided into strains with intestinal tropism (enterotoxigenic, enteropathogenic, enterohaemmorrhagic, verotoxigenic and enteroinvasive) and with extraintestinal tropism (uropathogenic and invasive). Invasive strains cause septicaemia and/or bacteraemia with localisations in different internal organs (systemic infections): If specific virulence properties and factors of strains with intestinal tropism are quite well known and described, those of strains with extraintestinal tropism are much less characterised, especially in animals. The purpose of this serie of review articles is to present the current knowledge on specific properties and factors of extraintestinal strains: adhesins and colonisation factors, transmucosal transfer and survival in blood and internal organs, toxicity. The fourth manuscript will deal with the invasive strains themselves, focusing on the necrotoxigenic strains. This first manuscript presents the current knowledge on the receptors, roles in pathogenesis, structures, genetic determinism and identification methods of fimbrial adhesins of P (Pap, Prs), S (Sfa, F1C, Sfr) and F17 (Vir, Att25, FY, 20K, G, Att111) families and of afimbrial adhesins of Afa family (Afa, Nfa, F1845, Bma, Dr...). [less ▲]

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See detailL’iniziativa I.E.D.P.E. sulla formazione dei coordinatori nei paesi europei : nel Belgio Francofono
Pirard, Florence ULg

in Percosi educativi di qualità per le bambine e i bambini in Italia e in Europa (2003)

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See detailDavid Malouf
Delrez, Marc ULg

in Schlager, Nigel; Lauer, Josh (Eds.) Contemporary Novelists (2003)

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See detailDégradation du cartilage et stress oxydatif chez des patients arthrosiques et souffrant d'arthrite rhumatoide
Deberg, Michelle; Labasse, A; Christgau, S et al

in Revue du Rhumatisme (2003), 70

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See detailSentence processing strategies in French-speaking children with SLI: a study of morphosyntactic cues
Maillart, Christelle ULg; Schelstraete, Marie-Anne

in Journal of Multilingual Communication Disorders (2003), 1

The sentence comprehension strategies used by children with speci®c language impairments (SLI) were examined within the framework of the Competition Model (Bates and MacWhinney, 1989). The experiment was ... [more ▼]

The sentence comprehension strategies used by children with speci®c language impairments (SLI) were examined within the framework of the Competition Model (Bates and MacWhinney, 1989). The experiment was a replication of Kail and Charvillat'experiment (1988) designed to investigate the use of different morphosyntactic cues (i.e. word order, clitic pronoun, verbal agreement) in sentence comprehension by children (4;6 ± 6;6) with normal language development. They found that French speaking children used mainly information on word order with a lesser reliance on the other cues (word order4clitic pronoun4verbal agreement). In the present study, the same experiment was replicated with 25 French speaking children with SLI. The results suggested that children with SLI had speci®c di culties in processing clitic pronouns, while verbal agreement could be processed (word order4verbal agreement4clitic pronoun). This pattern seems to be a speci®c strategy and not simply a delayed pro®le. In addition, our results were compatible with a theoretical account in terms of limitation in processing capacities. [less ▲]

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See detailMosaicism of Solid Gold supports the causality of a noncoding A-to-G transition in the determinism of the callipyge phenotype.
Smit, Maria; SEGERS, Karin ULg; Carrascosa, Laura Garcia et al

in Genetics (2003), 163(1), 453-6

To identify the callipyge mutation, we have resequenced 184 kb spanning the DLK1-, GTL2-, PEG11-, and MEG8-imprinted domain and have identified an A-to-G transition in a highly conserved dodecamer motif ... [more ▼]

To identify the callipyge mutation, we have resequenced 184 kb spanning the DLK1-, GTL2-, PEG11-, and MEG8-imprinted domain and have identified an A-to-G transition in a highly conserved dodecamer motif between DLK1 and GTL2. This was the only difference found between the callipyge (CLPG) allele and a phylogenetically closely related wild-type allele. We report that this SNP is in perfect association with the callipyge genotype. The demonstration that Solid Gold-the alleged founder ram of the callipyge flock-is mosaic for this SNP virtually proves the causality of this SNP in the determinism of the callipyge phenotype. [less ▲]

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