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See detailReconstruction OSEM de tomoscintigraphies acquises avec un collimateur sténopé et une trajectoire non-circulaire.
Seret, Alain ULg; Flérès, David; Defrise, Michel

in Médecine Nucléaire : Imagerie Fonctionnelle et Métabolique (2003), 27

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See detailMinitransplants: allogeneic stem cell transplantation with reduced toxicity.
Beguin, Yves ULg; Baron, Frédéric ULg

in Acta Clinica Belgica (2003), 58(1), 37-45

Allogeneic hematopoietic stem cell transplantation (HSCT) is used for the treatment of selected hematological malignancies. Its curative potential is based on two very different mechanisms, involving the ... [more ▼]

Allogeneic hematopoietic stem cell transplantation (HSCT) is used for the treatment of selected hematological malignancies. Its curative potential is based on two very different mechanisms, involving the conditioning regimen and the graft-versus-host reactions, respectively. The high-dose chemo-radiotherapy conditioning regimen is aimed at destroying tumor cells, ablating the host immune system (to prevent rejection) and eliminating the host bone marrow (to "make space" for donor stem cells). However, the definitive eradication of tumor cells is also largely mediated by an immune-mediated destruction of malignant cells by donor lymphocytes termed graft-versus-leukemia (GVL) or graft-versus-tumor (GVT) effect. However, because of its toxicity, conventional allogeneic HSCT is restricted to younger (< 55 years) and fitter patients. These observations led several groups to set up new (less toxic) transplant protocols based on a two step approach: first the use of immunosuppressive (but nonmyeloablative) conditioning regimens providing sufficient immunosuppression to achieve engraftment of allogeneic hematopoietic stem cells and, in a second step, destruction of malignant cells by the GVL effect. These transplants are called nonmyeloablative HSCT or reduced-conditioning HSCT or minitransplants. Preliminary results show that minitransplants are feasible with a relatively low transplant-related mortality (TRM) even in patients up to 70 years. In addition, strong anti-tumor responses are observed in several hematological malignancies as well as in some patients with renal cell carcinoma. As the benefits of minitransplants over alternative forms of treatment remain to be demonstrated, this strategy should be restricted to patients included in clinical trials. [less ▲]

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See detailCent ans de géographie humaine à Liège
Schmitz, Serge ULg

in Bulletin de la Société Géographique de Liège (2003), 43

Numéro spécial édité à l'occasion du centenaire de l'enseignement et de la géographie à l'Université de Liège et comportant les contributions des responsables des divers services de géographie au sein de ... [more ▼]

Numéro spécial édité à l'occasion du centenaire de l'enseignement et de la géographie à l'Université de Liège et comportant les contributions des responsables des divers services de géographie au sein de l'Université de Liège. [less ▲]

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See detailDistinct T cell subsets and cytokine production in cultures derived from transformation zone and squamous intraepithelial lesion biopsies of the uterine cervix.
Jacobs, Nathalie ULg; Renard, Isabelle; Al-Saleh, Walid et al

in American Journal of Reproductive Immunology (2003), 49(1), 6-13

PROBLEM: The characterization of lymphocytes issued from squamous intraepithelial lesions (SIL) and from the transformation zone (TZ), where the majority of SIL occur, is important to understand the role ... [more ▼]

PROBLEM: The characterization of lymphocytes issued from squamous intraepithelial lesions (SIL) and from the transformation zone (TZ), where the majority of SIL occur, is important to understand the role of immunity in SIL development. METHOD OF STUDY: We compared lymphocyte populations of the TZ and SIL with those of normal exocervix, using a technique allowing for the isolation of lymphocytes, either from the epithelium or from the underlying stroma of small biopsies. RESULTS: The majority of cells derived from the epithelium of all biopsies were CD8+ T cells. Some SIL-derived cultures were characterized by an increased proportion of activated TCRgammadelta+. The production of the immunosuppressive cytokine IL10 was significantly higher in lymphocyte cultures from the normal TZ in comparison with the exocervix. A decreased percentage of effector T cells was observed in cultures derived from the stroma of normal TZ (TCRgammadelta+) or SIL (CD8+) in comparison with the exocervix. CONCLUSIONS: Our results suggest that a low proportion of effector T cells and IL10 production could contribute to the predisposition of the TZ to the development of SIL and to the progression of SIL to cervical cancer. [less ▲]

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See detailLiège, Ville et Région. Documents cartographiques
Merenne-Schoumaker, Bernadette ULg; Van Overbeke, Marguerite; Mérenne, Emile et al

Cartographic material (2003)

Quatrième édition d'un petit atlas de Liège destiné principalement à des étudiants et des enseignants. Edité pour la première fois en 1987, cet atlas a été réédité en 1993, 1999 et 2003

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See detailRegulation of hypoxia-inducible factor-1alpha protein level during hypoxic conditions by the phosphatidylinositol 3-kinase/Akt/glycogen synthase kinase 3beta pathway in HepG2 cells.
Mottet, Denis ULg; Dumont, Valery; Deccache, Yann et al

in Journal of Biological Chemistry (2003), 278(33), 31277-85

Hypoxia initiates an intracellular signaling pathway leading to the activation of the transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 activity is regulated through different mechanisms ... [more ▼]

Hypoxia initiates an intracellular signaling pathway leading to the activation of the transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 activity is regulated through different mechanisms involving stabilization of HIF-1alpha, phosphorylations, modifications of redox conditions, and interactions with coactivators. However, it appears that some of these steps can be cell type-specific. Among them, the involvement of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway in the regulation of HIF-1 by hypoxia remains controversial. Here, we investigated the activation state of PI3K/Akt/glycogen synthase kinase 3beta (GSK3beta) in HepG2 cells. Increasing incubation times in hypoxia dramatically decreased both the phosphorylation of Akt and the inhibiting phosphorylation of GSK3beta. The PI3K/Akt pathway was necessary for HIF-1alpha stabilization early during hypoxia. Indeed, its inhibition was sufficient to decrease HIF-1alpha protein level after 5-h incubation in hypoxia. However, longer exposure (16 h) in hypoxia resulted in a decreased HIF-1alpha protein level compared with early exposure (5 h). At that time, Akt was no longer present or active, which resulted in a decrease in the inhibiting phosphorylation of GSK3beta on Ser-9 and hence in an increased GSK3beta activity. GSK3 inhibition reverted the effect of prolonged hypoxia on HIF-1alpha protein level; more stabilized HIF-1alpha was observed as well as increased HIF-1 transcriptional activity. Thus, a prolonged hypoxia activates GSK3beta, which results in decreased HIF-1alpha accumulation. In conclusion, hypoxia induced a biphasic effect on HIF-1alpha stabilization with accumulation in early hypoxia, which depends on an active PI3K/Akt pathway and an inactive GSK3beta, whereas prolonged hypoxia results in the inactivation of Akt and activation of GSK3beta, which then down-regulates the HIF-1 activity through down-regulation of HIF-1alpha accumulation. [less ▲]

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See detailLe japonisme en Belgique à la fin du XIXe siècle. Le cas d’Armand Rassenfosse
Bawin, Julie ULg

in 6e congrès de l’Association des cercles francophones d’histoire et d’archéologie de Belgique, LIIIe congrès de la fédération des cercles d’archéologie et d’histoire de Belgique (2003)

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See detailUltradispersity of diamond at the nanoscale
Raty, Jean-Yves ULg; Galli, G.

in Nature Materials (2003), 2(12), 792-795795

Nanometre-sized diamond has been found in meteorites, protoplanetary nebulae, and interstellar dusts, as well as in residues of detonation and in diamond films. Remarkably, the size distribution of ... [more ▼]

Nanometre-sized diamond has been found in meteorites, protoplanetary nebulae, and interstellar dusts, as well as in residues of detonation and in diamond films. Remarkably, the size distribution of diamond nanoparticles seems to be peaked around 2-5 nm, and to be largely independent of preparation conditions. We have carried out ab initio calculations of the stability of nanodiamond as a function of surface hydrogen coverage and of size. We have found that at about 3 nm, and for a broad range of pressures and temperatures, particles with bare, reconstructed surfaces become thermodynamically more stable than those with hydrogenated surfaces, thus preventing the formation of larger grains. Our findings provide an explanation of the size distribution of extraterrestrial and of terrestrial nanodiamond found in ultradispersed and ultracrystalline diamond films. They also provide an atomistic structural model of these films, based on the topology and structure of 2-3 nm diamond clusters consisting of a diamond core surrounded by a fullerene-like carbon network [less ▲]

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See detailMethodology for data acquisition, storage and treatment
Aubinet, Marc ULg; Clément, R.; Elbers, J. A. et al

in Valentini, R. (Ed.) Fluxes of Carbon, Water and Energy of European Forests (2003)

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See detailEtude rétrospective de quarante et un cas d’insuffisance mitrale chez le cheval (1995-2001).
Maurin, Emmanuel; Amory, Hélène ULg; Sandersen, Charlotte ULg

in Pratique Vétérinaire Equine (2003), 35

Une étude rétrospective portant sur quarante et un chevaux qui présentent une insuffisance cardiaque mitrale a été réalisée afin de mieux décrire cette affection et de préciser les répercussions médicales ... [more ▼]

Une étude rétrospective portant sur quarante et un chevaux qui présentent une insuffisance cardiaque mitrale a été réalisée afin de mieux décrire cette affection et de préciser les répercussions médicales et sportives liées à cette maladie. L'insuffisance mitrale est diagnostiquée grâce à des examens cliniques et échographiques, puis un questionnaire (évolution clinique et sportive) est adressé aux propriétaires des animaux. L’analyse de ces dossiers permet en particulier d’évaluer, d'une part, l’existence d’une association entre les signes d’insuffisance cardiaque congestive et certaines lésions cardio-vasculaires (lésions valvulaires, dilatation de l’atrium gauche, etc.) ou d'autres symptômes (fibrillation auriculaire, etc.), d'autre part, d’apprécier la corrélation éventuelle entre ces différents symptômes ou lésions et la survie des chevaux affectés. Les résultats ainsi que les biais possibles de cette étude sont ensuite discutés. [less ▲]

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See detailRedefining the role of psr in beta-lactam resistance and cell autolysis of Enterococcus hirae.
Sapunaric, Frédéric ULg; Franssen, Christine; Stefanic, Patrick et al

in Journal of Bacteriology (2003), 185(20), 5925-35

The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain ... [more ▼]

The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain R40 of Enterococcus hirae was investigated by disruption or substitution of the corresponding pbp5 and psr genes by Campbell-type recombination. The resulting modifications were confirmed by hybridization and PCR. The low susceptibility of E. hirae to beta-lactams was confirmed to be largely dependent on the presence of PBP5. However, against all expectations, inactivation of psr in ATCC 9790 or complementation of R40 cells with psr did not modify the susceptibility to benzylpenicillin or the growth and cell autolysis rates. These results indicated that the psr gene does not seem to be involved in the regulation of PBP5 synthesis and consequently in beta-lactam resistance or in the regulation of cell autolysis in E. hirae. [less ▲]

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See detailDiarrhée virale bovine et maladie des muqueuses
Pastoret, Paul-Pierre ULg; Hamers, C.; Dehan, Pierre ULg

in Lefevre, P.-C.; Blancou, J.; Chermette, R. (Eds.) Principales maladies infectieuses et parasitaires du bétail (2003)

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See detailDiagnosis and monitoring of pregnancy in sheep: reality and perspectives
El Amiri, Bouchra; Karen, Aly; Cognié, Yves et al

in Productions Animales (2003), 16(2), 79-90

The present review aims at presenting different methods for pregnancy diagnosis. Since these methods are to be used in the herds, precocity, sensitivity, specificity, accuracy to predict pregnant and non ... [more ▼]

The present review aims at presenting different methods for pregnancy diagnosis. Since these methods are to be used in the herds, precocity, sensitivity, specificity, accuracy to predict pregnant and non-pregnant ewes, and the possibility to determine fetal numbers are carefully analysed. The progesterone assay is accurate as early as day 17th after fecundation; but the fertilization day must be precisely known. The pregnancy specific or associated glycoproteins (PSPB/PAG) assay is highly sensitive and specific at day 22 in blood and at day 32 in milk samples. However, this assay does not allow to predict the fetal number. The ultrasonography B-mode used on 30 days of gestation gives very good or excellent performance in terms of sensitivity and specificity depending on the practitioner. The method is also able to predict the real time fetal number. [less ▲]

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See detailRezension zu Silke Schlichtmann: Geschlechterdifferenz in der Literaturrezeption um 1800? Zu zeitgenössischen Goethe-Lektüren
Pontzen, Alexandra ULg

in Arbitrium: Zeitschrift fuer Rezensionen zur germanistischen Literaturwissenschaft (2003)

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See detailLa varicelle et le zona ou les mystères de la latence d'un virus et de sa réactivation
Rentier, Bernard ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (2003), 158(3-4), 169-73174-5

Varicella-zoster virus is a Herpesvirus responsible for three distinct clinical features: chicken pox (varicella), shingles (herpes zoster) and post-zosterian pain (post-herpetic neuralgia). Neurological ... [more ▼]

Varicella-zoster virus is a Herpesvirus responsible for three distinct clinical features: chicken pox (varicella), shingles (herpes zoster) and post-zosterian pain (post-herpetic neuralgia). Neurological features of these diseases such as complications of chicken pox, viral latency in sensory ganglia and reactivation as shingles with concurrent and possibly subsequent prolonged pain, are the sequels of the invasion of the peripheral nervous system during primary infection. Prevention is achieved by vaccination with a live attenuated virus strain and therapy calls for specific antiviral agents. In many respects, vzv behaves differently from close relatives. In particular, viral latency in the nervous system is basically different from that of other Herpesviridae. The recent discovery of the expression of some viral regulatory proteins during latency, although it had always been considered that vzv latency was silent, and demonstration that these proteins are immunogenic open new avenues concerning the immune control of vzv reactivation. [less ▲]

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See detailVascular endothelial growth factor expression correlates with matrix metalloproteinases MT1-MMP, MMP-2 and MMP-9 in human glioblastomas.
Munaut, Carine ULg; Noël, Agnès ULg; Hougrand, Olivier ULg et al

in International Journal of Cancer = Journal International du Cancer (2003), 106(6), 848-55

Vascular endothelial growth factor (VEGF) is the major endothelial mitogen in central nervous system neoplasms and it is expressed in 64-95% of glioblastomas (GBMs). Tumour cells are the main source of ... [more ▼]

Vascular endothelial growth factor (VEGF) is the major endothelial mitogen in central nervous system neoplasms and it is expressed in 64-95% of glioblastomas (GBMs). Tumour cells are the main source of VEGF in GBMs whereas VEGF receptors (VEGFR-1, its soluble form sVEGFR-1, VEGFR-2 and neuropilin-1) are expressed predominantly by endothelial cells. Infiltrating tumour cells and newly-formed capillaries progress through the extracellular matrix by local proteolysis involving matrix metalloproteinases (MMPs). Recent studies have shown that VEGF expression and bioavailability can be modulated by MMPs. We reported previously that the expression of MT1-MMP in human breast cancer cells was associated with an enhanced VEGF expression. We used quantitative RT-PCR, Western blot, gelatin zymography and immunohistochemistry to study the expression of VEGF, VEGFR-1, VEGFR-2, sVEGFR-1, neuropilin-1, MT1-MMP, MMP-2, MMP-9 and TIMP-2 in 20 human GBMs and 5 normal brains. The expression of these MMPs was markedly increased in most GBMs with excellent correlation between mRNA and protein levels; activated forms of MMP-2 and MMP-9 were present in 8/18 and 7/18 of GBMs. A majority of GBMs (17/20) also expressed high levels of VEGF, as previously reported, with strong correlation between VEGF and MT1-MMP gene expression levels, and double immunostaining showed that VEGF and MT1-MMP peptides co-localize in tumour and endothelial cells. Our results suggest that the interplay between metalloproteinases and VEGF previously described in experimental tumours may also be operative in human GBMs. Because of its dual ability to activate MMP-2 and to up-regulate VEGF, MT1-MMP might be of central importance in the growth of GBMs and represent an interesting target for anti-cancer treatments. [less ▲]

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See detailAutoimmunity associated with anti-tumor necrosis factor alpha treatment in Crohn's disease: A prospective cohort study
Vermeire, S.; Noman, M.; Van Assche, G. et al

in Gastroenterology (2003), 125(1), 32-39

Background & Aims: Infliximab therapy is an effective approach to treating Crohn's disease. Development of antinuclear antibodies has been described in patients treated, but the size of the problem and ... [more ▼]

Background & Aims: Infliximab therapy is an effective approach to treating Crohn's disease. Development of antinuclear antibodies has been described in patients treated, but the size of the problem and the relationship with autoimmunity have not been investigated. We investigated the occurrence of antinuclear antibodies in 125 consecutive Crohn's disease patients and studied the relationship with symptoms of autoimmunity. Methods: Autoantibodies and clinical data were investigated before and 1, 2, and 3 months after infliximab infusion. If antinuclear antibodies were greater than or equal to1:80, further study of double-stranded DNA, single-stranded DNA, histones, and ENA was performed. Results: Cumulative antinuclear antibody incidence at 24 months was 71 of 125 (56.8%). Almost half of these patients developed antinuclear antibodies after the first infusion, and >75% became antinuclear antibody positive after fewer than 3 infusions. So far, only :15 of 71 patients have become seronegative, after a median of 12 months. Of 43 antinuclear antibody-positive patients who were further subtyped, 14 of 43 (32.6%) had double-stranded DNA, 17 (39.5%) had single-stranded DNA, 9 (20.9%) had antihistone, and 0% were ENA positive. Two patients (both antihistone and double-stranded DNA positive) developed drug-induced lupus without major organ damage, and I developed autoimmune hemolytic anemia. Antinuclear antibodies were associated with the female sex (odds ratio, 3.166; 95% confidence interval, 1.167-8.585; P = 0.024) and with papulosquamous or butterfly rash (odds ratio, 10.016; 95% confidence interval, 1.708-58.725; P = 0.011). Conclusions: The cumulative incidence of antinuclear antibodies was 56.8% after 24 months in this cohort of infliximab-treated Crohn's disease patients. Antinuclear antibodies persisted up to I year after the last infusion, and only a few patients became seronegative. Two patients developed drug-induced lupus erythematosus. Antinuclear antibodies were associated with the female sex and skin manifestations. [less ▲]

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See detailCloches et plaies des pieds.
Crosset, A.; Uhoda, Emmanuelle ULg; Pierard, Gérald ULg

in Pharma-Sphère (2003), 78

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