References of "2003"
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See detailMise en place d’une Sélection Assistée par Marqueurs dans les trois principales races bovines laitières françaises
Fritz, S.; Colleau, J. J.; Druet, Tom ULg et al

in Renc. Rech. Ruminants, 2003, 10 (2003)

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See detailScreening of histone deacetylase (HDAC) expression profiles in prostate cancer tissues leads to the identification of HDAC8 as a novel marker of smooth muscle differentiation
Waltregny, David ULg; Glénisson, Wendy; Tran, Syv Li et al

Conference (2003)

Background: Histone deacetylation mediated by histone deacetylases (HDAC) plays a key role in the regulation of gene expression. Inhibition of HDAC activity is associated with profound alterations in ... [more ▼]

Background: Histone deacetylation mediated by histone deacetylases (HDAC) plays a key role in the regulation of gene expression. Inhibition of HDAC activity is associated with profound alterations in cellular biology, such as cell growth arrest, apoptosis and differentiation. It has been hypothesized that altered HDAC expression and/or activity could play a role in cancer development and progression. Objectives: To investigate this possibility, we undertook a study in which we screened HDAC expression profiles in human prostate cancer. In addition, we sought to determine the specific biological effects of selected HDACs. Methods: A variety of techniques, including immunochemistry, Q-RT-PCR, and immunobloting, were used to examine the expression of several class I and class II HDACs in human prostate cancer cell lines and matched malignant and non-malignant prostate tissues. Analysis of the specific biological effects of selected HDACs was performed after knocking down their expression with the use of specific small interfering RNAs (siRNA) and/or after forcing their overexpression by transfection of the cDNAs of interest. Results: Normal prostate epithelial and stromal cells displayed distinct HDAC expression profiles, suggesting specific functions for these enzymes in the prostate. Human prostate cancer cells did not exhibit significant alterations in the abundance of any of the HDAC enzymes analyzed. One of the HDACs tested, herein referred to as HDACX, was specifically expressed, in vivo, by cells showing smooth muscle phenotype, including vascular and prostate smooth muscle cells and myoepithelial cells. HDACX expression co-localized with the expression of the specific smooth muscle marker alpha smooth muscle actin (alpha-SMA) in all human tissues tested. Nucleo-cytoplasmic fractionation experiments showed that HDACX was mainly expressed in the cytosol of human smooth muscle cells (HSMC) obtained from umbilical cord veins. Transfection experiments leading to overexpression of HDACX in mouse fibroblasts indicated that the enzyme was detected both in the cytosol and in the nucleus. By immunocytochemistry, it was observed that HDACX pattern of expression was reminiscent of actin stress fibers, suggesting that this HDAC may be involved in the regulation of the smooth muscle cell cytoskeleton. Trichostatin A (TSA), a specific global HDAC inhibitor, as well as specific HDACX siRNAs, were able to completely prevent the TGFß1-induced differentiation of fibroblasts into myofibroblasts, as assessed by alpha-SMA abundance. Forced downregulation of HDACX by siRNAs in HSMC caused a dramatic reduction in their contraction capacity, as determined with the use of hydrated collagen lattices contraction assays. Conclusions : HDACs diplay distinct expression profiles among normal prostate epithelial and stromal cells. The abundance of the HDACs analyzed in this study is not altered in prostate cancer. Further experiments are required to determine whether the activity of these enzymes is altered in prostate cancer. We have identified for the first time an HDAC that (i) is specifically expressed in cells showing smooth muscle phenotype, (ii) is required for myofibroblastic differentiation, and (iii) is involved in smooth muscle cell contraction. This HDAC may become a target for the therapy of several pathological conditions, including chronic inflammation and cancer, in which myofibroblasts play a major role. [less ▲]

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See detailReview of James Procter's Dwelling Places: Postwar Black British Writing
Ledent, Bénédicte ULg

in Moving Worlds : A Journal of Transcultural Writings (2003), 3(2), 138-139

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See detailLe positionnement multidimensionnel : principes et applications
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (2003), (1), 1-32

This note describes some multidimensional scaling methods for two-way, one-mode tables and for three-way, two-mode tables. The methods are illustrated by an example processed by PROC MDS of SAS software.

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See detail‘De kanker van een diepgewortelde droom’. Over Marcel van Maele
Spinoy, Erik ULg

in Van Maele, Marcel (Ed.) Scherpschuttersfeest (2003)

This is a general introduction to the work of the Flemish novelist and poet Marcel van Maele.

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See detailLes facteurs de virulence des alphaherpèsvirus
Muylkens, Benoît ULg; Meurens, François; Schynts, Frédéric et al

in Virologie (2003), 7

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See detailNumerical simulations of tissue ingrowth and differentiation around a loaded titanium implant in a bone chamber
Van Oosterwyck, Hans; Geris, Liesbet ULg; Vander Sloten, Jos et al

in Proceedings of the symposium on integrated systems in dental healthcare (2003)

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See detailSpeed of Sound Measurements of the Third Metacarpal Bone in Young Exercising Thoroughbred Racehorses
Carstanjen, B.; Lepage, O. M.; Hars, O. et al

in BONE (2003), 33

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See detailThe NDWI as a drought index applied to Belgium and Heilongjiang
Tychon, Bernard ULg; Pêcheur, Céline; Ozer, Pierre ULg

in Tychon, Bernard (Ed.) Belgian and Chinese Crop Growth Monitoring Systems: comparison, adaptation and improvement (2003)

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See detailLes cours d'instruments semi-collectifs : pistes d'action et de réflexion. Fiches pédagogiques
Giot, Bernadette ULg; Baye, Ariane ULg

Book published by Service de Pédagogie expérimentale de l'Université de Liège - Ministère de la Communauté française, AGERS (2003)

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See detailHygro-thermal application conditions and adhesion
Courard, Luc ULg; Darimont, Anne ULg; Degeimbre, Robert ULg et al

in Seidler, Peter (Ed.) Industrial Floors 03 (2003)

Coatings are designed to protect concrete against environmental aggression. Water is one of the aggressive parameters able to deteriorate the concrete structure. The objectives of this paper are to ... [more ▼]

Coatings are designed to protect concrete against environmental aggression. Water is one of the aggressive parameters able to deteriorate the concrete structure. The objectives of this paper are to present the effects of water (i) during application of coating on the concrete substrate and (ii) pull-off test. Semi-permanent moisty state produced by absorption from the uncoated face, total immersion or diffusion of water from the back face of the substrate are analysed. Water vapour diffusion and influence of hydrophobic treatment are also investigated. A classification based on increasing influence of the tests and application conditions on adhesion is proposed and clearly points out the negative effect of capillary action from the uncoated face. [less ▲]

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See detailGuidelines for clinical studies assessing the efficacy of drugs for the management of acute low back pain
Devogelaer, J. P.; Dreiser, R. L.; Abadie, Eric ULg et al

in Clinical and Experimental Rheumatology (2003), 21(6, NOV-DEC), 691-694

In this paper we propose guidelines for clinical trials aimed at assessing the efficacy of drugs for acute non-specific low back pain (LBP) with or without radicular pain, preliminary to their approval ... [more ▼]

In this paper we propose guidelines for clinical trials aimed at assessing the efficacy of drugs for acute non-specific low back pain (LBP) with or without radicular pain, preliminary to their approval and registration. To this end, consensus statements were obtained from a group of experts in the fields of rheumatology, clinical medicine, public health and epidemiology. EBM resources were systematically used as references. [less ▲]

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See detailConnaissances générales en histoire, actualités, économie
Leclercq, Dieudonné ULg; Georges, François ULg

in Leclercq, Dieudonné (Ed.) Diagnostic cognitif et métacognitif au seuil de l'université : le projet Mohican menée par les 9 universités de la Communauté française Wallonie-Bruxelles (2003)

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See detailSelective adsorption of dioxins and PCBs from marine oils on activated carbon
De Meulenaer, Bruno; Maes, Jeroen; Van Heerswynghels, Peter et al

in Organohalogen Compounds (2003)

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