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See detailCrystal structure of the sensor domain of the BlaR penicillin receptor from Bacillus licheniformis
Kerff, Frédéric ULg; Charlier, Paulette ULg; Colombo, Maria Louisa et al

in Biochemistry (2003), 42(44), 12835-12843

As in several staphylococci, the synthesis of the Bacillus licheniformis 749/I beta-lactamase is an inducible phenomenon regulated by a signal-transducing membrane protein BlaR. The C-terminal domain of ... [more ▼]

As in several staphylococci, the synthesis of the Bacillus licheniformis 749/I beta-lactamase is an inducible phenomenon regulated by a signal-transducing membrane protein BlaR. The C-terminal domain of this multimodular protein is an extracellular domain which specifically recognizes beta-lactam antibiotics. When it binds a beta-lactam, a signal is transmitted by the transmembrane region to the intracellular loops. In response, the hydrolytic activity of the BlaR large cytoplasmic L3 loop is induced, and a cascade of reactions is generated, leading to the transcription of the beta-lactamase gene. Here, we describe the crystal structure of the extracellular penicillin-receptor domain of BlaR (residues 346-601) at 2.5 Angstrom resolution in order to understand why this domain, whose folding is very similar to that of class D beta-lactamases, behaves as a highly sensitive penicillin-binding protein rather than a beta-lactamase. Two residues of the BlaR C-terminal domain, Thr452 and Thr542, modify the hydrophobic characteristic of the class D beta-lactamase active site. Both residues seem to be in part responsible for the lack of beta-lactamase activity of the BlaR protein due to the stability of the acyl-enzyme. Although further experimental data are needed to fully understand the transmembrane induction process, the comparison of the BlaR sensor domain structure with those of class D beta-lactamase complexes and penicillin-binding proteins provides interesting elements to hypothesize on possible signal transmission mechanisms. [less ▲]

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See detailSize heterogeneity, cannibalism and competition in cultured predatory fish larvae: biotic and abiotic influences
Kestemont, P.; Jourdan, S.; Houbart, M. et al

in Aquaculture (2003), 227(1-4), 333-356

Growth heterogeneity is a central problem in larviculture and especially in predatory species. It can be influenced by a wide range of intrinsic and environmental factors, of which the respective ... [more ▼]

Growth heterogeneity is a central problem in larviculture and especially in predatory species. It can be influenced by a wide range of intrinsic and environmental factors, of which the respective influences are largely unknown. The role of non-interactive (temperature, day length, light intensity, food availability and composition) and interactive factors (stocking density, initial size heterogeneity, hatching time) on growth, survival and size heterogeneity was measured in larvae and post-larvae of the European seabass Dicentrarchus labrax and Eurasian perch Perca fluviatilis. Embryos hatching later than others were found less competitive than those hatching earlier in both species, but to a greater extent in perch. By contrast, the final size heterogeneity in both species was independent from the initial size heterogeneity or recurrent size sorting. High stocking density had a positive effect on perch larvae, no effect on seabass larvae and a negative impact on the post-larvae of both species, owing principally to density-dependent access to food. Day length and light intensity produced contrasting results in larvae and post-larvae. Larvae of both species performed better under bright light and continuous day length. Post-larvae of seabass performed equally well at different light levels but did better under short day lengths, whereas post-larvae of perch were unaffected by day length but performed better under reduced light levels. Increasing food availability resulted in increasing performance of perch larvae, although cannibalism was higher for submaximal than for maintenance rations. The similarity between the two species at the larval stage, and differences at the post-larval stage can be accounted for by the increasing specialisation towards specific environments and niches. Regarding the impact of rearing factors on growth, survival and size heterogeneity, interactive variables impacted essentially on growth and survival, whereas size heterogeneity was chiefly influenced by non-interactive variables, either directly or indirectly via interactive mechanisms such as cannibalism and size-dependent mortality. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailTypologie floristique des cours d’eau luxembourgeois
Sossey Alaoui, Khadija ULg; Thoen, Daniel ULg; Bouxin, Guy et al

Poster (2003, November 08)

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See detailApplication of bioencapsulated proteinase and peptides for wound healing
Markvicheva, E; Dugina, T.; Grandfils, Christian ULg et al

Conference (2003, November 08)

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See detailCampagne de mesure des odeurs sur le CET réhabilité de Belderbusch (Montzen) Enquête sur les nuisances olfactives
Nicolas, Jacques ULg; Craffe, Flavien

Report (2003)

The study is made in the frame of a follow-up monitoring of all landfill sites in Wallonia, initiated by the Ministry of Environment and managed by ISSeP. The present report concerns the restored site of ... [more ▼]

The study is made in the frame of a follow-up monitoring of all landfill sites in Wallonia, initiated by the Ministry of Environment and managed by ISSeP. The present report concerns the restored site of Belderbusch. However, as first field inspections revealed no odour, the usual measurement methodology was not applied and a short survey in the neighbourhood population was conducted. [less ▲]

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See detailCharacterization of the primary sonic muscles in Carapus acus (Carapidae): a multidisciplinary approach
Parmentier, Eric ULg; Gennotte, Vincent ULg; Focant, Bruno et al

in Proceedings of the Royal Society B : Biological Sciences (2003), 270(1530), 2301-2308

Sound production in carapid fishes results from the action of extrinsic muscles that insert into the swim bladder. Biochemical, histochemical and morphological techniques were used to examine the sonic ... [more ▼]

Sound production in carapid fishes results from the action of extrinsic muscles that insert into the swim bladder. Biochemical, histochemical and morphological techniques were used to examine the sonic muscles and compare them with epaxial muscles in Carapus acus. Sonic fibres are thicker than red and thinner than white epaxial fibres, and sonic fibres and myofibrils exhibit an unusual helicoidal organization: the myofibrils of the centre are in a straight line whereas they are more and more twisted towards the periphery. Sonic muscles have both features of red (numerous mitochondria, high glycogen content) and white (alkali-stable ATPase) fibres. They differ also in the isoforms of the light chain (LC3) and heavy chain (HC), in having T tubules at both the Z-line and the A–I junction and in a unique parvalbumin isoform (PAI) that may aid relaxation. All these features lead to the expression of two assumptions about sound generation: the sonic muscle should be able to perform fast and powerful contractions that provoke the forward movement of the forepart of the swim bladder and the stretching and ‘flapping’ of the swim bladder fenestra; the helicoidal organization allows progressive drawing of the swim bladder fenestra which emits a sound when rapidly released in a spring-like manner. [less ▲]

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See detail« Immigration, Intégration et Citoyenneté au regard de la Communication du 3 juin 2003 »
Martiniello, Marco ULg

Scientific conference (2003, November 07)

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See detailL’histoire sacrifiée sur l’autel des langues ?
Jadoulle, Jean-Louis ULg

Article for general public (2003)

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See detailAislamiento de Escherichia coli enterohemorrágico (EHEC) de una cría de guanaco en Argentina.
Mercado, E.C.; Rodriguez, Sabrina ULg; Parreño, V. et al

Poster (2003, November 05)

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See detailLe rêve pavillonnaire et la logique du développement durable
Halleux, Jean-Marie ULg

Conference (2003, November 04)

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See detailPrevalence of Echinococcus multilocularis in the red fox (Vulpes vulpes) in southern Belgium
Losson, Bertrand ULg; Kervyn, Thierry; Detry, Jacques et al

in Veterinary Parasitology (2003), 117(1-2), 23-28

Between June 1998 and February 2002,709 red foxes killed in Wallonia (south of Belgium) were available for parasitological examination of the gut. The identification of Echinococcus multilocularis was ... [more ▼]

Between June 1998 and February 2002,709 red foxes killed in Wallonia (south of Belgium) were available for parasitological examination of the gut. The identification of Echinococcus multilocularis was based on morphological data. E. multilocularis adults were observed in 20.2% of the animals. The analysis of data revealed marked differences between the geological areas of Wallonia; the highest prevalence (33%) was found in the Ardenne and the lowest (0%) on the Plateau de Herve. Host gender and the collection season had no effect on the prevalence. However, the latter was significantly higher in juveniles (<8 months of age). The geographical distribution of E. multilocularis in Belgium is much wider than originally thought. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailRemote sensing of the proton aurora characteristics from IMAGE-FUV
Bisikalo, D. V.; Shematovich, V. I.; Gérard, Jean-Claude ULg et al

in Annales Geophysicae [= ANGEO] (2003), 21

The combination of simultaneous global images of the north polar region obtained with the IMAGE-FUV imaging system makes it possible to globally map the properties of the electron and proton auroral ... [more ▼]

The combination of simultaneous global images of the north polar region obtained with the IMAGE-FUV imaging system makes it possible to globally map the properties of the electron and proton auroral precipitation. The SI12 imager, which observes the Doppler-shifted Lyman-a [less ▲]

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See detailJupiter's main auroral oval observed with HST-STIS
Grodent, Denis ULg; Clarke, J. T.; Kim, J. et al

in Journal of Geophysical Research. Space Physics (2003), 108(A11),

An extended series of FUV images obtained on 7 days during winter 2000-2001, with fixed pointing, yielded highly accurate tracking of emisson features as Jupiter rotated. They provided newly detailed ... [more ▼]

An extended series of FUV images obtained on 7 days during winter 2000-2001, with fixed pointing, yielded highly accurate tracking of emisson features as Jupiter rotated. They provided newly detailed measurements of the degree of corotation of auroral emissions and their variations with changing central meridian longitude. This 2-month data set provides a statistical average location of the auroral emission and leads to the definition of new "reference ovals.'' The overall auroral morphology pattern is shown to be fixed in System-III longitude and unchanged over a 5-year period. When arranged in central meridian longitude ranges, the images show a significant contraction of the northern main oval as the central meridian longitude increases from 115 to 255degrees. The main auroral oval brightness is globally very stable in comparison with its terrestrial counterpart. It is shown to vary with magnetic local time, increasing from noon to dusk and then decreasing again in the magnetic evening. Hectometric emissions observed simultaneously with Galileo and Cassini reveal interplanetary shocks propagating outward from the Sun which may be related to the contraction of the main auroral oval observed in the HST images taken on 14 December 2000. In addition, we find that a brightening and a significant contraction of the main oval observed on 13 January 2001 corresponded to a time of increased solar wind dynamic pressure. [less ▲]

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See detailSimulated annealing for complex portfolio selection problems
Crama, Yves ULg; Schyns, Michael ULg

in European Journal of Operational Research (2003), 150(3), 546-571

This paper describes the application of a simulated annealing approach to the solution of a complex portfolio selection model. The model is a mixed integer quadratic programming problem which arises when ... [more ▼]

This paper describes the application of a simulated annealing approach to the solution of a complex portfolio selection model. The model is a mixed integer quadratic programming problem which arises when Markowitz' classical mean-variance model is enriched with additional realistic constraints. Exact optimization algorithms run into difficulties in this framework and this motivates the investigation of heuristic techniques. Computational experiments indicate that the approach is promising for this class of problems. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailPharmacodynamics of follicle stimulating hormone (FSH) in postmenopausal women during pulsed estrogen therapy: Evidence that FSH release and synthesis are controlled by distinct pathways
Christin-Maitre, S.; Laveille, C.; Collette, Julien ULg et al

in Journal of Clinical Endocrinology and Metabolism (2003), 88(11), 5405-5413

17beta-Estradiol (E2) exerts negative feedback effects at the hypothalamo-pituitary level on serum FSH. This study investigated the effects of repeated daily administration of intranasal E2 (S21400) on ... [more ▼]

17beta-Estradiol (E2) exerts negative feedback effects at the hypothalamo-pituitary level on serum FSH. This study investigated the effects of repeated daily administration of intranasal E2 (S21400) on the pharmacokinetics (PK) of E2 and estrone (E1) and the pharmacodynamics (PD) of FSH and assessed the PK/PD relationship between E2 and FSH using population model-dependent analysis. Postmenopausal volunteers (n = 24) received according to a balanced cross-over design, two 28-d treatments separated by a 2-month wash-out period: 300 mug E2, either alone or combined with oral dydrogesterone (20 mg/d) during the last 14 d of one of the treatments. Absorption of E2 was rapid, with maximal plasma concentrations at 10-30 min, returning to postmenopausal levels within 12 h. Over the 24-h period, FSH levels showed a U curve, with a minimum around 8 h after E2 administration. Moreover, over the treatment period, FSH basal values decreased by 17% between d 1 and 14 and an additional 5% between d 14 and 28. A PK/PD model described these short- and mid-term effects, possibly reflecting separate regulation mechanisms by E2 on FSH release and biosynthesis, respectively. The administration of progestin had no influence on E1, E2, and FSH model parameters. This study suggests that daily transient tissue exposure to E2 after pulsed estrogen therapy elicits short- and mid-term effects on the gonadotropin axis. [less ▲]

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See detailEvolution of the polarization of the optical afterglow of the γ-ray burst GRB030329
Greiner, Jochen; Klose, Sylvio; Reinsch, Klaus et al

in Nature (2003), 426

The association of a supernova with GRB030329 strongly supports the `collapsar' model of γ-ray bursts, where a relativistic jet forms after the progenitor star collapses. Such jets cannot be spatially ... [more ▼]

The association of a supernova with GRB030329 strongly supports the `collapsar' model of γ-ray bursts, where a relativistic jet forms after the progenitor star collapses. Such jets cannot be spatially resolved because γ-ray bursts lie at cosmological distances; their existence is instead inferred from `breaks' in the light curves of the afterglows, and from the theoretical desire to reduce the estimated total energy of the burst by proposing that most of it comes out in narrow beams. Temporal evolution of the polarization of the afterglows may provide independent evidence for the jet structure of the relativistic outflow. Small-level polarization (~1-3 per cent) has been reported for a few bursts, but its temporal evolution has yet to be established. Here we report polarimetric observations of the afterglow of GRB030329. We establish the polarization light curve, detect sustained polarization at the per cent level, and find significant variability. The data imply that the afterglow magnetic field has a small coherence length and is mostly random, probably generated by turbulence, in contrast with the picture arising from the high polarization detected in the prompt γ-rays from GRB021206 (ref. 18). [less ▲]

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See detailGeneva photometry of HD 129929 (Aerts+, 2004)
Aerts, C.; Waelkens, C.; Daszynska-Daszkiewicz, J. et al

in SIMBAD (2003), 341

We have gathered and analysed a timeseries of 1493 high-quality multicolour Geneva photometric data of the B3V {beta} Cep star Hd 129929. The dataset has a time base of 21.2 years. The occurrence of a ... [more ▼]

We have gathered and analysed a timeseries of 1493 high-quality multicolour Geneva photometric data of the B3V {beta} Cep star Hd 129929. The dataset has a time base of 21.2 years. The occurrence of a beating phenomenon is evident from the data. We find evidence for the presence of at least six frequencies, among which we see components of two frequency multiplets with an average spacing of ~0.0121c/d which points towards very slow rotation. This result is in agreement with new spectroscopic data of the star and also with previously taken UV spectra. We provide the amplitudes of the six frequencies in all seven photometric filters. The metal content of the star is Z=0.018+/-0.004. All these observational results will be used to perform detailed seismic modelling of this massive star in a subsequent paper. (1 data file). [less ▲]

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See detailOptical and mechanical design of a straylight rejection baffle for CoRoT
Plesseria, Jean-Yves ULg; Mazy, Emmanuel ULg; Defise, Jean-Marc ULg et al

in Society of Photo-Optical Instrumentation Engineers (SPIE) Conference Series (2003, November 01)

The COROT mission is part of the program "Petites Missions" of CNES (French space agency). It implies international cooperation between France, Belgium, Germany, Austria, Spain and the European Space ... [more ▼]

The COROT mission is part of the program "Petites Missions" of CNES (French space agency). It implies international cooperation between France, Belgium, Germany, Austria, Spain and the European Space Agency (ESA). COROT aims to perform astroseismology measurements and to detect exoplanets. Long duration observations of stars will be used to detect periodic variations with an afocal telescope followed by a dioptric objective and 4 CCDs. Due to the orbit of the spacecraft (low altitude polar orbit) and even if the observation are performed in a direction perpendicular to orbit plane, the measurements can be disturbed by the straylight reflected on the earth (albedo) that can generate periodic perturbation. CSL is in charge of the design and procurement, with the help of Belgian industries, of a baffle and its protective cover that will be mounted on top of the afocal entrance telescope. The requirements are very stringent from the optical point of view as well as from the mechanical point of view. The rejection of the baffle must be of the order of 10[SUP]13[/SUP] for field angles above 20 degrees while the allocated mass is 19 kilograms. [less ▲]

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See detailOverexpression of the soluble vascular endothelial growth factor receptor in preeclamptic patients: Pathophysiological consequences
Tsatsaris, V.; Goffin, Frédéric ULg; Munaut, Carine ULg et al

in Journal of Clinical Endocrinology and Metabolism (2003), 88(11), 5555-5563

Several growth factors such as vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF) are involved in the placental vascular development. We investigated whether dysregulation in ... [more ▼]

Several growth factors such as vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF) are involved in the placental vascular development. We investigated whether dysregulation in the VEGF family may explain the defective uteroplacental vascularization characterizing preeclampsia. We compared pregnancies complicated by early onset severe preeclampsia or intrauterine growth retardation to normal pregnancies. Maternal plasma, placentas, and placental bed biopsies were collected. The mRNA levels of VEGF-A, PlGF, and their receptors were quantified in placentas and placental beds. Levels of VEGF-A, PlGF, and soluble VEGF receptor (VEGFR) were assessed in maternal plasma. In compromised pregnancies, elevated levels of VEGF-A and VEGFR-1 mRNAs may reflect the hypoxic status of the placenta. On contrast, the membrane-bound VEGFR-1 was decreased in the placental bed of preeclamptic patients. Preeclampsia was associated with low levels of circulating PlGF and increased levels of total VEGF-A and soluble VEGFR-1. Free VEGF-A was undetectable in maternal blood. Immunohistochemical studies revealed that VEGF-A and PlGF were localized in trophoblastic cells. Altogether, our results suggest two different pathophysiological mechanisms associated with preeclampsia. The first one is related to an overproduction of competitive soluble VEGFR-1 that may lead to suppression of VEGF-A and PlGF effects. The second one is the down-regulation of its membrane bound form (VEGFR-1) in the placental bed, which may result in the defective uteroplacental development. [less ▲]

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