Cytoplasmic I kappa B alpha increases NF-kappa B-independent transcription through binding to histone deacetylase (HDAC) 1 and HDAC3

in Journal of Biological Chemistry (2003), 278(47), 46541-46548

IkappaBalpha is an inhibitory molecule that sequesters NF-kappaB dimers in the cytoplasm of unstimulated cells. Upon stimulation, NF-kappaB moves to the nucleus and induces the expression of a variety of ... [more ▼]

IkappaBalpha is an inhibitory molecule that sequesters NF-kappaB dimers in the cytoplasm of unstimulated cells. Upon stimulation, NF-kappaB moves to the nucleus and induces the expression of a variety of genes including IkappaBalpha. This newly synthesized IkappaBalpha also translocates to the nucleus, removes activated NF-kappaB from its target genes, and brings it back to the cytoplasm to terminate the phase of NF-kappaB activation. We show here that IkappaBalpha enhances the transactivation potential of several homeodomain-containing proteins such as HOXB7 and Pit-1 through a NF-kappaB-independent association with histone deacetylase (HDAC) 1 and HDAC3 but not with HDAC2, -4, -5, and -6. IkappaBalpha bound both HDAC proteins through its ankyrin repeats, and this interaction was disrupted by p65. Immunofluorescence experiments demonstrated further that IkappaBalpha acts by partially redirecting HDAC3 to the cytoplasm. At the same time, an IkappaBalpha mutant, which lacked a functional nuclear localization sequence, interacted very efficiently with HDAC1 and -3 and intensively enhanced the transactivation potential of Pit-1. Our results support the hypothesis that the NF-kappaB inhibitor IkappaBalpha regulates the transcriptional activity of homeodomain-containing proteins positively through cytoplasmic sequestration of HDAC1 and HDAC3, a mechanism that would assign a new and unexpected role to IkappaBalpha. [less ▲]

Chiuse poetiche e senso della fine. Spunti per una tipologia

Scientific conference (2003, November 20)

Communication of Introduction to the Workshop: « A Common European Immigration Policy : A Common Policy for Different Problems ? »

Scientific conference (2003, November 20)

VERFAHREN ZUM ERHÖHEN DES ÖLGEHALTES IN PFLANZEN

Patent (2003)

The invention relates to methods for increasing the oil content in plants, preferably in the seeds of plants, by expression of glycerol-3-phosphatdehydrogenases (G3PDH) from yeast, preferably from ... [more ▼]

The invention relates to methods for increasing the oil content in plants, preferably in the seeds of plants, by expression of glycerol-3-phosphatdehydrogenases (G3PDH) from yeast, preferably from Saccharomyces cerevisiae. The invention also relates to expression constructs for the expression of G3PDH yeast in plants, preferably in the seeds of plants, transgenic plants expressing G3PDH, and to the use of said transgenic plants in the production of foodstuffs, feed, seeds, pharmaceuticals or fine chemicals, especially in the production of oils. [less ▲]

Communication Entitled:« Engaging Politicians, the Public and Civil Society »

Scientific conference (2003, November 19)

Prise en compte du coût des infrastructures dans la gestion de l’urbanisme résidentiel

Conference (2003, November 19)

Développement d'algorithmes génétiques et application à des problèmes complexes d'optimisation

Scientific conference (2003, November 18)

Optimisation de la spécificité de l'encodage pour le diagnostic précoce de la maladie d'Alzheimer

Scientific conference (2003, November 18)

Traffic Effects of Driver Assistance Systems – The Approach within INVENT

(2003, November 16)

Within the German Research Initiative INVENT, the partial project „Verkehrliche Wirkung, Recht und Akzeptanz (VRA)“ deals with traffic effects, legal aspects, acceptance and economics of driver assistance ... [more ▼]

Within the German Research Initiative INVENT, the partial project „Verkehrliche Wirkung, Recht und Akzeptanz (VRA)“ deals with traffic effects, legal aspects, acceptance and economics of driver assistance systems. This presentation gives examples for the consulting services of VRA as well as for the early simulations of a driver assistance system. Such simulations vary in scale from basic functions over driving situations and traffic situations to traffic scenarios. Results are presented for the basic functions and driving situations for a stop-and-go extension of adaptive cruise control. [less ▲]

Palladium-silver sol-gel catalysts for selective hydrodechlorination of 1,2-dichloroethane into ethylene - IV. Deactivation mechanism and regeneration

in Journal of Catalysis (2003), 220(1), 215-225

The activity and selectivity of a 1.9% Pd-3.7% Ag/SiO2 catalyst during selective hydrodechlorination of 1,2-dichloroethane into ethylene have been followed in the course of time. The deactivation curves ... [more ▼]

The activity and selectivity of a 1.9% Pd-3.7% Ag/SiO2 catalyst during selective hydrodechlorination of 1,2-dichloroethane into ethylene have been followed in the course of time. The deactivation curves show two distinct periods: an initial period at the very beginning of catalyst operation, that is, during the first 20 operating hours, characterized by a sharp decrease of the rates of both reactions involved in the process (1,2-dichloroethane hydrodechlorination and undesired ethylene hydrogenation) and a second period, after the first 20 operating hours and investigated for more than 600 h, characterized by a clearly slower deactivation. During the initial period, the hydrodechlorination rate decreases less quickly than the hydrogenation rate and this results in a fast increase of ethylene selectivity which reaches a maximum at the end of this period. After the initial period, the hydrodechlorination rate decreases faster than the hydrogenation rate and this results in a slow decrease of ethylene selectivity. A comparison between the physico-chemical properties of the fresh catalyst and of the deactivated one suggests a deactivation mechanism by poisoning or coking of silver sites at the surface of the active Pd-Ag alloy particles whereas palladium sites would remain intact. The selectivity evolution during the second period, that is, after the very first operating hours, is in agreement with such a mechanism. However, the sharp ethylene selectivity increase during the initial operating hours suggests, in addition to the deactivation by silver sites disappearance, the presence of a further nonidentified phenomenon. The characterization of the catalyst after a regeneration treatment including an oxidation step followed by a reduction step shows that this treatment allows restoration near to the physico-chemical properties of the fresh catalyst. (C) 2003 Elsevier Inc. All rights reserved. [less ▲]