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See detailThe potential of the beta-Microprobe, an intracerebral radiosensitive probe, to monitor the [(18)F]MPPF binding in the rat dorsal raphe nucleus.
Zimmer, L.; Pain, F.; Mauger, G. et al

in European Journal of Nuclear Medicine and Molecular Imaging (2002), 29(9), 1237-47

The aim of this study was to demonstrate the ability of a recently developed beta(+)-range sensitive intracerebral probe (beta-Microprobe) to measure the binding kinetics of [(18)F]MPPF, a well-documented ... [more ▼]

The aim of this study was to demonstrate the ability of a recently developed beta(+)-range sensitive intracerebral probe (beta-Microprobe) to measure the binding kinetics of [(18)F]MPPF, a well-documented 5-HT(1A) serotoninergic receptor ligand, in the dorsal raphe nucleus (DRN) of the anaesthetised rat. This midbrain nucleus presents a high concentration of 5-HT(1A) receptors known to be implicated in the effects of antidepressants. The difficulty confronting this study lay in the fact that the dimensions of the DRN are smaller than the detection volume of the beta-Microprobe. In the first part of the study, we studied the feasibility of this measurement from a theoretical point of view by autoradiography and a Monte Carlo simulation. We determined the optimal beta-Microprobe location close to the DRN and verified that this configuration allowed accurate determination of [(18)F]MPPF specific binding in the nucleus. In the second part of our study, we measured the in vivo time-concentration curves of [(18)F]MPPF binding in the DRN in comparison with the cerebellum. The specificity of [(18)F]MPPF binding in the DRN was confirmed by its displacement after non-labelled 5-HT(1A)antagonist injection (MPPF or WAY-100635). Moreover, we verified the feasibility of using beta-Microprobe monitoring and simultaneous validation by microdialysis to study the effect of an increase in extracellular serotonin, induced by fenfluramine injection, on [(18)F]MPPF binding in the DRN. Our theoretical simulations, confirmed by our experimental results, demonstrate the ability of this new device to monitor in vivo the binding of [(18)F]MPPF in the DRN of anaesthetised rodents. [less ▲]

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See detailChansons liégeoises de conscrits. La mise en page de la voix populaire
Droixhe, Daniel ULg

in Leclerc, M.-D.; Robert, A. (Eds.) Chansons de colportage (2002)

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See detailAtteintes neuromusculaires aiguës de réanimation (ANMAR) : étude électrophysiologique prospective de 56 cas
BARTSCH, Valérie ULg; TOMASELLA, Marco ULg; Zeevaert, Bernard et al

in Neurophysiologie Clinique = Clinical Neurophysiology (2002)

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See detailLa conformation bouchère des agneaux. Etude d'après la variabilité génétique entre races
Laville, E.; Bouix, J.; Sayd, T. et al

in INRA Productions Animales (2002), 15(1), 53-66

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See detailAdvantages and drawbacks of nanospray for studying noncovalent protein-DNA complexes by mass spectrometry
Gabelica, Valérie ULg; Vreuls, Christelle ULg; Filée, Patrice ULg et al

in Rapid Communications in Mass Spectrometry : RCM (2002), 16(18), 1723-1728

The noncovalent complexes between the BlaI protein dimer (wild-type and GM2 mutant) and its double-stranded DNA operator were studied by nanospray mass spectrometry and tandem mass spectrometry (MS/MS ... [more ▼]

The noncovalent complexes between the BlaI protein dimer (wild-type and GM2 mutant) and its double-stranded DNA operator were studied by nanospray mass spectrometry and tandem mass spectrometry (MS/MS). Reproducibility problems in the nanospray single-stage mass spectra are emphasized. The relative intensities depend greatly on the shape of the capillary tip and on the capillary-cone distance. This results in difficulties in assessing the relative stabilities of the complexes simply from MS' spectra of protein-DNA mixtures. Competition experiments using MS/MS are a better approach to determine relative binding affinities. A competition between histidine-tagged BlaIWT (BlaIWTHis) and the GM2 mutant revealed that the two proteins have similar affinities for the DNA operator, and that they co-dimerize to form heterocomplexes. The low sample consumption of nanospray allows MS/MS spectra to be recorded at different collision energies for different charge states with 1 muL of sample. The MS/MS experiments on the dimers reveal that the GM2 dimer is more kinetically stable in the gas phase than the wild-type dimer. The MS/MS experiments on the complexes shows that the two proteins require the same collision energy to dissociate from the complex. This indicates that the rate-limiting step in the monomer loss from the protein-DNA complex arises from the breaking of the protein-DNA interface rather than the protein-protein interface. The dissociation of the protein-DNA complex proceeds by the loss of a highly charged monomer (carrying about two-thirds of the total charge and one-third of the total mass). MS/MS experiments on a heterocomplex also show that the two proteins BlaIWTHis and BlaIGM2 have slightly different charge distributions in the fragments. This emphasizes the need for better understanding the dissociation mechanisms of biomolecular complexes. [less ▲]

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See detailLa gestion des eaux par bassins hydrographiques
Pâques, Michel ULg; Leprince, Sylviane ULg

in Aménagement - Environnement (2002)

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See detailDes pratiques aux performances : la littératie chez les jeunes de 15 ans
Lafontaine, Dominique ULg

in Lettre de la DFLM (La) (2002), 30

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See detailUse of very high resolution (VHR) imagery for control and updating of legal land occupation maps in urban areas.
Binard, Marc ULg

in Final report summaries: Earth Observation by Satellite TELSAT4 (2002)

The ageing of the regional sector pla ns as also the recent changes in map content as defined by the new Walloon code on land planning of 27 November 1997 justify the analysis of new updating ... [more ▼]

The ageing of the regional sector pla ns as also the recent changes in map content as defined by the new Walloon code on land planning of 27 November 1997 justify the analysis of new updating methodologies. Most important in regard to the updating strategy to be adopted is the question of the availability of land for additional housing developments. This study therefore focuses on the assessment of available construction sites and some of their characteristics. The study area coves map sheet 50/6 of the Malmedy-Saint-Vith sector. The original method proposed in this case study is based on computer assisted photo interpretation of digital ortho-photomaps, combined with some sector map information. The method is user-friendly and leads immediately to quantitative assessments, also facilities of conceiving and editing of thematic maps. Further useful information is obtained by combining the map information with a digital terrain model of the area. Research, on the one hand, confirms the availability of land for additional housing developments, and on the other reveals the consequences of bad urbanisation policies (promotion and development of linear housing developments, insufficient control over quarrying activities, etc.) the "new code" introduces new town planning requirements (more detailed zoning analysis taking into account the environmental aspects, creation of protected areas, impact surveys, ground occupancy estimation) and strengthens the prospect of using this methodology further on. [less ▲]

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See detailHydrothermal Synthesis of Alluaudite in the Na-Mn-Fe2+-Fe3+-P-O System
Hatert, Frédéric ULg

in Journal of Conference Abstracts (2002), 7(1), 44

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See detailLes finances communales dans les années ’90 : une décennie de répit ?
Husson, Jean-François ULg

in de Callataÿ, Etienne (Ed.) La fin du déficit budgétaire. Analyse de l’évolution récente des finances publiques belges (1990-2000) – Histoire des finances publiques en Belgique t. VI (2002)

Ce chapitre retrace l'évolution des finances des communes belges au cours des années 1990.

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See detailLa bibliothèque du Prieuré du Val-Des-Ecoliers à Houffalize : 8 volumes conservés à la Bibliothèque Royale de Belgique
Adam, Renaud ULg

in Archives et Bibliothèques de Belgique = Archief en Bibliotheekwezen in Belgie (2002), 73

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See detailCompte rendu de I. Galster : La naissance du « phénomène Sartre »
Cormann, Grégory ULg

in Revue Philosophique de Louvain (2002), 1-2

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See detailRecommendations for a standardized report for adult transthoracic echocardiography: a report from the American Society of Echocardiography's Nomenclature and Standards Committee and Task Force for a Standardized Echocardiography Report.
Gardin, Julius M.; Adams, David B.; Douglas, Pamela S. et al

in Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography (2002), 15(3), 275-90

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See detailMétabolisme des lipides chez la levure: catabolisme peroxysomal des acides gras et applications biotechnologiques
Waché, Y.; Aguedo, Mario ULg; Nicaud, J.M. et al

in Regard sur la Biochimie (2002), 4

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See detailHIV resistance to antiretroviral drugs: Mechanisms, genotypic and phenotypic resistance testing in clinical practice
Blaise, Pierre ULg; Clevenbergh, P.; Vaira, Dolorès ULg et al

in Acta Clinica Belgica (2002), 57(4, Jul-Aug), 191-201

HIV resistance to antiretroviral agents is a major contributory cause of treatment failure. The dynamics of HIV replication, together with patient-, physician-, and drug-related factors, lead to emergence ... [more ▼]

HIV resistance to antiretroviral agents is a major contributory cause of treatment failure. The dynamics of HIV replication, together with patient-, physician-, and drug-related factors, lead to emergence of HIV resistant strains in most of the patients. Phenotypic assays look for an increase in the antiretroviral drug (ARV) concentration that inhibits 50% of the growth of the tested HIV strain (IC50), comparatively with a reference strain cultivated in parallel. Genotypic tests detect resistance mutations in the reverse transcriptase and protease genes by comparing the gene sequences of a resistant virus to those of a wildtype strain that has previously been described. The efficacy of each ARV class and each individual ARV is threatened by specific mutations and resistance mechanisms. In retrospective studies of genotypic or phenotypic resistance testing, baseline resistance tests results were correlated with virological outcomes. There is some evidence from prospective studies that resistance testing may have some benefits when used to choose salvage regimens. However, problems in the areas of test interpretation, patient compliance, availability of active drugs, and technical test performance limit the usefulness of resistance testing in clinical practice. This article reviews the mechanisms underlying HIV resistance, the principles of phenotypic and genotypic tests, and the use of these tests in clinical practice. [less ▲]

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