References of "2002"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailTight binding of the antitumor drug ditercalinium to quadruplex DNA
Carrasco, Carolina; Rosu, Frédéric ULg; Gabelica, Valérie ULg et al

in Chembiochem : A European Journal of Chemical Biology (2002), 3(12), 1235-1241

The structural selectivity of the DNA-binding antitumor drug ditercalinium was investigated by competition dialysis with a series of nineteen different DNA substrates. The 7H-pyridocarbazole dimer was ... [more ▼]

The structural selectivity of the DNA-binding antitumor drug ditercalinium was investigated by competition dialysis with a series of nineteen different DNA substrates. The 7H-pyridocarbazole dimer was found to bind to double stranded DNA with a preference for GC rich species but can in addition form stable complex with triplex and quadruplex structures. The preferential interaction of the drug with four-stranded DNA structures was independantly confirmed by electrospray mass spectrometry and a detailed analysis of the binding reaction was performed by surface plasmon resonance (SPR) spectrospray. The BIAcore SPR study showed that the kinetic parameters for the interaction of ditercalinium with the human telomeric quadruplex sequence are comparable to those measured with a duplex sequence. Slow association and dissociation were observed with both the quadruplex and duplex structures. The newly discovered preferential binding of ditercalinium to the antiparallel quadruplex sequence d(AG(3)[T(2)AG(3)](3)) provides new perspective for the design of drugs that can bind to human telomeres. [less ▲]

Detailed reference viewed: 94 (11 ULg)
Full Text
See detailRenforcement de la capacité de gestion des ressources en eau dans l'agriculture moyennant des outils de suivi-évaluation (Burkina Faso): Rapport Technique (2002)
Wellens, Joost ULg; Compaore, Nestor; Dieng, Babacar et al

Report (2002)

L’approche proposée par le projet existe dans la combinaison des besoins en formation des ingénieurs de haut niveau de l’EIER d’une part avec les besoins de la DRH-HB d’autre part. En utilisant le système ... [more ▼]

L’approche proposée par le projet existe dans la combinaison des besoins en formation des ingénieurs de haut niveau de l’EIER d’une part avec les besoins de la DRH-HB d’autre part. En utilisant le système d’information géographique de la DRH-HB le projet a comme objectif de développer des outils informatiques qui peuvent aider à gérer mieux l’eau et qui ont la capacité de formuler une estimation précise du rendement des cultures pluviales dans la région pendant la saison. Afin de garantir des effets multiplicateurs et spin-off, et de disposer d’un nombre suffisant de chercheurs qui travailleront dans le cadre du projet sans exagérer les coûts, les outils seront développés dans le cadre des mémoires de fin d’études des meilleurs étudiants africains des quatorze états subsahariens de l’EIER et des étudiants des Universités flamandes. [less ▲]

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailCompte rendu de ARDON, Vie de Benoît d'Aniane, Abbaye de Bellefontaine, 2001
George, Philippe ULg

in Moyen Age (Le) (2002)

Detailed reference viewed: 16 (2 ULg)
Full Text
Peer Reviewed
See detailMise au point d'un modèle cartographique pour la description des stations forestières en Ardenne belge.
Claessens, Hugues ULg; Lejeune, Philippe ULg; Cuvelier, M. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(4), 209-220

The paper presents an original method dealing with the forest site types delineation. The suggested method consists in integrating a typological key in a GIS aiming at producing a thematic map that ... [more ▼]

The paper presents an original method dealing with the forest site types delineation. The suggested method consists in integrating a typological key in a GIS aiming at producing a thematic map that describes forest site types. Data used are the soil map of Belgium (digitized at the scale 1:20,000) and a digital elevation model built from a topographic map (scale 1:10,000). The typological key is mainly based on the methodology used by Thill et al. (1988) in the site types system for central Ardenne, the potential vegetation map of Sougnez and Dethioux (1975) and the ecoregion map of Delvaux and Galoux (1962). In that respect, site types are closely linked to the soil map and the phytosociological classification. So, they can be connected to the afforestation guide and different phytosociological and autecological studies concerning forest species. It is then possible to map the potential habitats or the site potentialities related to tree species. The key is valid for the Ardenne ecoregion located in Southern Belgium (elevation higher than 300 m). It has to be validated through an intensive use in the field, taking into account its imprecision linked to the types of collected data, chieffly those being digitized. The integration of such a tool in a SIG can be considered as an original way in terms of integrated forest management or forest sites description in the context of the project “Natura 2000” launched by the European Union. The study has been carried out within the framework of an experimental integrated management project concerning the Saint-Hubert forest (17,000 ha). [less ▲]

Detailed reference viewed: 225 (38 ULg)
Full Text
See detailÉcriture et système de prescription. L’exemple du “Lecteur du Petit Journal” (Zola, 1865)
Durand, Pascal ULg

in Jurt, J.; Einfalt, M. (Eds.) Le texte et le contexte. Analyses du champ littéraire français (XIXe et XXe siècle) (2002)

Detailed reference viewed: 55 (5 ULg)
See detailClinical and genom-wide linkage studies of a multigénérational family with acromegaly and the exclusion of requiem gene
Khoo, S. K.; Chan, S.; Pendek, R. et al

in The Endocrine Society's 84th Annual Meeting - Abstract book (2002)

Detailed reference viewed: 8 (0 ULg)
Full Text
See detailTroubles psychiques liés aux greffes d'organes
Triffaux, Jean-Marc ULg; maurette, jean louis; dozot, jean pol et al

in Encyclopédie Médico-Chirurgicale (2002)

Detailed reference viewed: 424 (6 ULg)
Full Text
See detailLa raison névrotique . Individualisme et société
Jacquemain, Marc ULg

Book published by Labor (2002)

Le développement de valeurs "post-matérialistes" dans le monde développé depuis les années 1970 présente une double face : d'une part, l'émergence de valeurs politiques plus sensibles aux finalités ... [more ▼]

Le développement de valeurs "post-matérialistes" dans le monde développé depuis les années 1970 présente une double face : d'une part, l'émergence de valeurs politiques plus sensibles aux finalités ultimes de l'action; d'autre part, le développement d'un individualisme compétitif. La tension entre ces deux faces opposées d'une même évolution est pour beaucoup dans le "malaise " des sociétés actuelles, que mettent en évidence la majorité des sociologues [less ▲]

Detailed reference viewed: 336 (23 ULg)
Full Text
Peer Reviewed
See detailExperimental Validation Of The Constant Level Method For Identification Of Non-Linear Multi-Degree-Of-Freedom Systems
Dimitriadis, Grigorios ULg

in Journal of Sound & Vibration (2002), 258(5), 829-845

System identification for non-linear dynamical systems could find use in many applications such as condition monitoring, finite element model validation and determination of stability. The effectiveness ... [more ▼]

System identification for non-linear dynamical systems could find use in many applications such as condition monitoring, finite element model validation and determination of stability. The effectiveness of existing non-linear system identification techniques is limited by various factors such as the complexity of the system under investigation and the type of non-linearities present. In this work, the constant level identification approach, which can identify multi-degree-of-freedom systems featuring any type of non-linear function, including discontinuous functions, is validated experimentally. The method is shown to identify accurately an experimental dynamical system featuring two types of stiffness non-linearity. The full equations of motion are also extracted accurately, even in the presence of a discontinuous non-linearity. [less ▲]

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailExercise stress echocardiography in healthy horses
Amory, Hélène ULg; Brihoum, M; Debrue, M et al

in Proceedings of the 41st Annual Congress of the British Equine Veterinary Association (BEVA) (2002)

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailUncoupling proteins outside the animal and plant kingdoms: functional and evolutionary aspects.
Sluse, Francis ULg; Jarmuszkiewicz, W.

in FEBS Letters (2002), 510

The appearance of intracellular oxidative phosphorylation at the time of acquisition of mitochondria in Eukarya was very soon accompanied by the emergence of uncoupling protein, a carrier specialized in ... [more ▼]

The appearance of intracellular oxidative phosphorylation at the time of acquisition of mitochondria in Eukarya was very soon accompanied by the emergence of uncoupling protein, a carrier specialized in free fatty acid-mediated H(+) recycling that can modulate the tightness of coupling between mitochondrial respiration and ATP synthesis, thereby maintaining a balance between energy supply and demand in the cell and defending cells against damaging reactive oxygen species production when electron carriers of the respiratory chain become over-reduced. The simultaneous occurrence of redox free energy-dissipating oxidase, which has the same final effect, could be related to the functional interactions between both dissipative systems [less ▲]

Detailed reference viewed: 10 (1 ULg)
See detailThe Slowly Pulsating B Star HD 147394
Briquet, Maryline ULg; Noels-Grötsch, Arlette ULg; Aerts, C. et al

in Aerts, Conny; Bedding, Timothy R.; Christensen-Dalsgaard, Jørgen (Eds.) RADIAL AND NONRADIAL PULSATIONS AS PROBES OF STELLAR PHYSICS (2002)

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailATP-driven, Na(+)-independent inward Cl- pumping in neuroblastoma cells
Bettendorff, Lucien ULg; Lakaye, Bernard ULg; Margineanu, Ilca et al

in Journal of Neurochemistry (2002), 81(4), 792-801

In immature neurones, the steady-state intracellular Cl- concentration [Cl-](i) is generally higher than expected for passive distribution, and this is believed to be due to Na(+)-K(+)-2Cl(-) co-transport ... [more ▼]

In immature neurones, the steady-state intracellular Cl- concentration [Cl-](i) is generally higher than expected for passive distribution, and this is believed to be due to Na(+)-K(+)-2Cl(-) co-transport. Here, we show that N2a neuroblastoma cells, incubated in HEPES-buffered NaCl medium maintain a [Cl-](i) around 60 mm, two- to threefold higher than expected for passive distribution at a membrane potential of - 49 mV. When the cells were transferred to a Cl(-) -free medium, [Cl-](i) decreased quickly (t(1/2) < 5 min), suggesting a high Cl- permeability. When the intracellular ATP concentration was reduced to less than 1 mm by metabolic inhibitors, the initial rate of (36) Cl- uptake was strongly inhibited (60-65%) while steady-state [Cl-](i) decreased to 24 mm, close to the value predicted from the Nernst equilibrium. Moreover, after reduction of [ATP](i) and [Cl-](i) by rotenone, the subsequent addition of glucose led to a reaccumulation of Cl-, in parallel with ATP recovery. Internal bicarbonate did not affect Cl- pumping, suggesting that Cl-/HCO(3)(-) exchange does not significantly contribute to active transport. Likewise, Na(+) -K(+) -2Cl(-) co-transport also appeared to play a minor role: although mRNA for the NKCC1 form of the co-transporter was detected in N2a cells, neither the initial rate of (36)Cl- uptake nor steady-state [Cl-](i) were appreciably decreased by 10 microm bumetanide or replacement of external Na(+) by choline. These results suggest that a highly active ATP-dependent mechanism, distinct from Na(+) -K(+) -2Cl(-) co-transport, is responsible for most of the inward Cl- pumping in N2a cells. [less ▲]

Detailed reference viewed: 36 (2 ULg)
Full Text
Peer Reviewed
See detailElevated atmospheric CO2 in open top chambers increases net nitrification and potential denitrification
Carnol, Monique ULg; Hogenboom, L.; Jach, M. E. et al

in Global Change Biology (2002), 8

The control of soil nitrogen (N) availability under elevated atmospheric CO2 is central to predicting changes in ecosystem carbon (C) storage and primary productivity. The effects of elevated CO2 on ... [more ▼]

The control of soil nitrogen (N) availability under elevated atmospheric CO2 is central to predicting changes in ecosystem carbon (C) storage and primary productivity. The effects of elevated CO2 on belowground processes have so far attracted limited research and they are assumed to be controlled by indirect effects through changes in plant physiology and chemistry. In this study, we investigated the effects of a 4-year exposure to elevated CO2 (ambient + 400 mumol mol(-1) ) in open top chambers under Scots pine (Pinus sylvestris L) seedlings on soil microbial processes of nitrification and denitrification. Potential denitrification (DP) and potential N-2 O emissions were significantly higher in soils from the elevated CO2 treatment, probably regulated indirectly by the changes in soil conditions (increased pH, C availability and NO3 (-) production). Net N mineralization was mainly accounted for by nitrate production. Nitrate production was significantly larger for soil from the elevated CO2 treatment in the field when incubated in the laboratory under elevated CO2 (increase of 100%), but there was no effect when incubated under ambient CO2 . Net nitrate production of the soil originating from the ambient CO2 treatment in the field was not influenced by laboratory incubation conditions. These results indicate that a direct effect of elevated atmospheric CO2 on soil microbial processes might take place. We hypothesize that physiological adaptation or selection of nitrifiers could occur under elevated CO2 through higher soil CO2 concentrations. Alternatively, lower microbial NH4 assimilation under elevated CO2 might explain the higher net nitrification. We conclude that elevated atmospheric CO2 has a major direct effect on the soil microbial processes of nitrification and denitrification despite generally higher soil CO2 concentrations compared to atmospheric concentrations. [less ▲]

Detailed reference viewed: 40 (0 ULg)
Full Text
Peer Reviewed
See detailEffects of repetitive transcranial magnetic stimulation on visual evoked potentials in migraine.
Bohotin, V.; Fumal, Arnaud ULg; Vandenheede, M. et al

in Brain : A Journal of Neurology (2002), 125(Pt 4), 912-22

Between attacks, migraine patients are characterized by potentiation instead of habituation of stimulation-evoked cortical responses. It is debated whether this is due to increased or decreased cortical ... [more ▼]

Between attacks, migraine patients are characterized by potentiation instead of habituation of stimulation-evoked cortical responses. It is debated whether this is due to increased or decreased cortical excitability. We have studied the changes in visual cortex excitability by recording pattern-reversal visual evoked potentials (PR-VEP) after low- and high-frequency repetitive transcranial magnetic stimulation (rTMS), known respectively for their inhibitory and excitatory effect on the cortex. In 30 patients (20 migraine without, 10 with aura) and 24 healthy volunteers, rTMS of the occipital cortex was performed with a focal figure-of-eight magnetic coil (Magstim). Nine hundred pulses were delivered randomly at 1 or 10 Hz in two separate sessions. Stimulus intensity was set to the phosphene threshold or to 110% of the motor threshold if no phosphenes were elicited. Before and after rTMS, PR-VEP were averaged sequentially in six blocks of 100zztieresponses during uninterrupted 3.1 Hz stimulation. In healthy volunteers, PR-VEP amplitude was significantly decreased in the first block after 1 Hz rTMS and the habituation normally found in successive blocks after sustained stimulation was significantly attenuated. In migraine patients, 10 Hz rTMS was followed by a significant increase of first block PR-VEP amplitude and by a reversal to normal habituation of the potentiation (or dishabituation) characteristic of the disorder. This effect was similar in both forms of migraine and lasted for at least 9 min. There were no significant changes of PR-VEP amplitudes after 1 Hz rTMS in migraineurs and after 10 Hz rTMS in healthy volunteers, nor after sham stimulation. The recovery of a normal PR-VEP habituation pattern after high-frequency rTMS is probably due to activation of the visual cortex and the dishabituation in healthy volunteers to cortical inhibition. We conclude, therefore, that the deficient interictal PR-VEP habituation in migraine is due to a reduced, and not to an increased, pre-activation excitability level of the visual cortex. [less ▲]

Detailed reference viewed: 36 (8 ULg)
Full Text
Peer Reviewed
See detailDe Mably à Marin. Dans le dédale ornemental de l'imprimeur parisien Quillau (XVIIIe siècle)
Droixhe, Daniel ULg

in Livre et l'Estampe (Le) (2002), 48(158), 43-69

Detailed reference viewed: 73 (1 ULg)
Full Text
Peer Reviewed
See detailThe plant pathogenesis-related PR-10 proteins
Elbez, Mounira; Kevers, Claire ULg; Hamdi, Saïd et al

in Acta Botanica Gallica : Bulletin de la Société Botanique de France (2002), 149(4), 415-444

The PR-10 protein family contains proteins related to pathogenesis and allergenic proteins. They are acidic proteins, with an intracellular localization, usually members of a multigene family and ... [more ▼]

The PR-10 protein family contains proteins related to pathogenesis and allergenic proteins. They are acidic proteins, with an intracellular localization, usually members of a multigene family and ubiquitous among plant kingdom. They contain a highly conserved motif, identified as a phosphate binding site. Even if the biological function of these proteins is still unknown, many indicators seem to favour the hypothesis of an RNAse activity. Moreover, one of them seems to be a cytokinin specific binding protein. Besides, PR-10 protein expression induction under cytokinin treatment was observed with the Catharanthus roseus CrPR-10a protein. Here, we present the evidence of a significative increase of endogenous cytokinin contents in leaves of transgenic Tobacco plants over-expressing the CrPR-10a protein. [less ▲]

Detailed reference viewed: 229 (6 ULg)