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See detailDual effects of an extra disulfide bond on the activity and stability of a cold-adapted alpha-amylase
D'Amico, Salvino ULg; Gerday, Charles ULg; Feller, Georges ULg

in Journal of Biological Chemistry (2002), 277(48), 46110-46115

Chloride-dependent alpha-amylases constitute a well conserved family of enzymes thereby allowing investigation of the characteristics of each member to understand, for example, relevant properties ... [more ▼]

Chloride-dependent alpha-amylases constitute a well conserved family of enzymes thereby allowing investigation of the characteristics of each member to understand, for example, relevant properties required for environmental adaptation. In this context, we have constructed a double mutant (Q58C/A99C) of the cold-active and heat-labile alpha-amylase from the Antarctic bacterium Pseudoalteromonas haloplanktis, defined on the basis of its strong similarity with the mesophilic enzyme from pig pancreas. This mutant was characterized to understand the role of an extra disulfide bond specific to warm-blooded animals and located near the entrance of the catalytic cleft. We show that the catalytic parameters of the mutant are drastically modified and similar to those of the mesophilic enzyme. Calorimetric studies demonstrated that the mutant is globally stabilized (DeltaDeltaG = 1.87 kcal/mol at 20 degrees C) when compared with the wild-type enzyme, although the melting point (T-m) was not increased. Moreover, fluorescence quenching experiments indicate a more compact structure for the mutated a-amylase. However, the strain imposed on the active site architecture induces a 2-fold higher thermal inactivation rate at 45 degreesC as well as the appearance of a less stable calorimetric domain. It is concluded that stabilization by the extra disulfide bond arises from an enthalpy-entropy compensation effect favoring the enthalpic contribution. [less ▲]

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See detailLifetime measurements and calculations in singly ionized ytterbium
Biémont, Emile ULg; Quinet, Pascal ULg; Dai, Z. W. et al

in Journal of Physics B-Atomic Molecular and Optical Physics (2002), 35(22), 4743-4749

New radiative lifetimes, measured by time-resolved laser-induced fluorescence spectroscopy, are reported for five Rydberg states of singly ionized ytterbium. Free Yb+ ions were produced in a laser-induced ... [more ▼]

New radiative lifetimes, measured by time-resolved laser-induced fluorescence spectroscopy, are reported for five Rydberg states of singly ionized ytterbium. Free Yb+ ions were produced in a laser-induced plasma. The experimental results have been compared with HFR calculations, taking core-polarization effects into account, and a good agreement (within 25%) between theory and experiment is observed for four levels. HFR results are also proposed for np (n less than or equal to 7) and nf (n less than or equal to 8) Rydberg states and compared with available data. [less ▲]

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See detailThe influence of Space Weather and of the ionosphere on Global Navigation Satellite Systems
Warnant, René ULg; Jodogne, Jean-Claude; Nebdi, Hamid

Conference given outside the academic context (2002)

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See detailLe poids des Églises et des sectes au Congo-Kinshasa
Melice, Anne ULg

Conference given outside the academic context (2002)

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See detailLes orientations stratégiques de la Coopération Universitaire au Développement
Marchal, Jean ULg

(2002, November 28)

Chargée, dès 1997, à préparer et à mettre en œuvre les responsabilités nouvelles des universités francophones de Belgique dans le cadre de la coopération universitaire au développement, la C.U.D. n’a ... [more ▼]

Chargée, dès 1997, à préparer et à mettre en œuvre les responsabilités nouvelles des universités francophones de Belgique dans le cadre de la coopération universitaire au développement, la C.U.D. n’a cessé d’identifier, de définir, d’adapter, d’affiner ses orientations stratégiques. Ce processus a été celui d’un apprentissage continu : de nombreuses évaluations internes à la C.U.D., et à ses groupes de travail, une concertation continue avec les autorités publiques, le relais des institutions universitaires du sud, ont permis à la C.U.D. de consolider, tout au long de l’actuel plan quinquennal 1998-2002, les orientations stratégiques qui guident ses programmes et qui constitueront la base des travaux du prochain plan quinquennal. Dans la présente contribution, sont décrites très brièvement les principales orientations stratégiques qui servent de lignes directrices à la C.U.D. [less ▲]

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See detailQuelle est la place de la mémoire dans l'apprentissage du français langue seconde
Lucchini, Silvia ULg

Conference given outside the academic context (2002)

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See detailPerinatal Group B Streptococcal Disease Prevention, Interactive session
Melin, Pierrette ULg

Conference (2002, November 23)

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See detailUne analyse exploratoire des effets des formations continues sur les salariés
Conter, Bernard; Maroy, Christian; Orianne, Jean-François ULg

Conference (2002, November 22)

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See detailUne analyse exploratoire des effets des formations continues sur les salariés
Conter, Bernard; Maroy, Christian; Orianne, Jean-François ULg

Conference (2002, November 22)

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See detailSameleven in the 21ste eeuw
Martiniello, Marco ULg

Scientific conference (2002, November 22)

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See detailContrôle des produits agroalimentaires par analyse d'images
Leemans, Vincent ULg; Kleynen, Olivier; Destain, Marie-France ULg

in Nouveaux capteurs pour la maîtrise de la qualité des produits agroalimentaires - Etat de la recherche européenne (2002, November 22)

Présentation de méthodes utilisées dans un processus de contrôle de qualité basé sur l'analyse d'image, en se focalisant sur la couleur et la forme des produits et leur classification

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See detailTraffic Effects of Driver Assistance Systems – The Approach within INVENT-VRA
Benz, T.; Christen, Fréderic ULg; Lerner, G. et al

in Telematik im Kraftfahrzeug (2002, November 21)

Within the German Research Initiative INVENT, the partial project „Verkehrliche Wirkung, Recht und Akzeptanz (VRA)“ deals with traffic effects, legal aspects, acceptance and economics of driver assistance ... [more ▼]

Within the German Research Initiative INVENT, the partial project „Verkehrliche Wirkung, Recht und Akzeptanz (VRA)“ deals with traffic effects, legal aspects, acceptance and economics of driver assistance systems. The major focus is to accompany the development of such systems rather than evaluating the systems at a later stage. This ensures that wrong development paths are avoided from the beginning. This presentation gives examples for the consulting services of VRA as well as for the early simulations of a driver assistance system. Such simulations vary in scale from basic functions to driving situations, then to traffic situations and finally to traffic scenarios. Results are presented for the basic functions and driving situations for a stop-and-go extension of adaptive cruise control. [less ▲]

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See detailAn X-ray investigation of the NGC 346 field in the Small Magellanic Cloud - I. The luminous blue variable HD 5980 and the NGC 346 cluster
Nazé, Yaël ULg; Hartwell, J. M.; Stevens, I. R. et al

in Astrophysical Journal (2002), 580(Part 1), 225-234

We present results from a Chandra observation of the NGC 346 cluster. This cluster contains numerous massive stars and is responsible for the ionization of N66, the most luminous H II region and the ... [more ▼]

We present results from a Chandra observation of the NGC 346 cluster. This cluster contains numerous massive stars and is responsible for the ionization of N66, the most luminous H II region and the largest star formation region in the SMC. In this first paper we will focus on the characteristics of the main objects of the field. The NGC 346 cluster itself shows only relatively faint X-ray emission (with L-X(unabs) similar to 1.5x10(34) ergs s(-1)), tightly correlated with the core of the cluster. In the field also lies HD 5980, a luminous blue variable star in a binary (or possibly a triple) system that is detected for the first time at X-ray energies. The star is X-ray bright, with an unabsorbed luminosity of L-X(unabs) similar to 1.7x10(34) ergs s(-1), but needs to be monitored further to investigate its X-ray variability over a complete 19 day orbital cycle. The high X-ray luminosity may be associated either with colliding winds in the binary system or with the 1994 eruption. HD 5980 is surrounded by a region of diffuse X-ray emission, which is a supernova remnant. While it may be only a chance alignment with HD 5980, such a spatial coincidence may indicate that the remnant is indeed related to this peculiar massive star. [less ▲]

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See detailAtmospheric Trace Molecule Spectroscopy (ATMOS) experiment version 3 data retrievals
Irion, Fredrick W.; Gunson, Michael R.; Toon, Geoff C. et al

in Applied Optics (2002), 41(33), 6968-6979

Version 3 of the Atmospheric Trace Molecule Spectroscopy (ATMOS) experiment data set for some 30 trace and minor gas profiles is available. From the IR solar-absorption spectra measured during four Space ... [more ▼]

Version 3 of the Atmospheric Trace Molecule Spectroscopy (ATMOS) experiment data set for some 30 trace and minor gas profiles is available. From the IR solar-absorption spectra measured during four Space Shuttle missions (in 1985, 1992, 1993, and 1994), profiles from more than 350 occultations were retrieved from the upper troposphere to the lower mesosphere. Previous results were unreliable for tropospheric retrievals, but with a new global-fitting algorithm profiles are reliably returned down to altitudes as low as 6.5 km (clouds permitting) and include notably improved retrievals of H2O, CO, and other species. Results for stratospheric water are more consistent across the ATMOS spectral filters and do not indicate a net consumption of H-2 in the upper stratosphere. A new sulfuric-acid aerosol product is described. An overview of ATMOS Version 3 processing is presented with a discussion of estimated uncertainties. Differences between these Version 3 and previously reported Version 2 ATMOS results are discussed. Retrievals are available at http://atmos.jpl.nasa.gov/atmos. (C) 2002 Optical Society of America. [less ▲]

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See detailL’engagement du chercheur en sciences sociales
Geuens, Geoffrey ULg

Scientific conference (2002, November 19)

Detailed reference viewed: 35 (17 ULg)
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See detailEffect of molecular weight of the reactive precursors in melt reactive blending
Yin, Zhihui; Koulic, Christian; Jeon, H. K. et al

in Macromolecules (2002), 35(24), 8917-8919

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See detailFormation à la rééducation vocale des dysphonies et dysodies chez l'adulte
Morsomme, Dominique ULg

Conference given outside the academic context (2002)

Detailed reference viewed: 16 (2 ULg)