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See detailDevelopment of a mobile compound composition tester. Phase II : Segregation of tires at retreading stage / Functional specification for a handheld prototype
Maternova, Jindriska; Nicolas, Jacques ULg

Report (2000)

The object of the second phase of the study is a technical support/advise to the construction of a portable prototype equipment for on site segregation of tires at the retreading stage. The final ... [more ▼]

The object of the second phase of the study is a technical support/advise to the construction of a portable prototype equipment for on site segregation of tires at the retreading stage. The final equipment aims at identifying the composition type of the rubber of the treads : either high natural rubber content (>60%) or high synthetic rubber content. An additional goal of the study is to elaborate the functional specifications for a handheld compound analyser to be coupled to the Goodyear viscoelasticity tester. [less ▲]

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See detailGenotypic Characterization of Enteropathogenic Escherichia Coli (Epec) Isolated in Belgium from Dogs and Cats
Goffaux, F.; China, B.; Janssen, L. et al

in Research in Microbiology (2000), 151(10), 865-71

Enteropathogenic Escherichia coli (EPEC) are isolated from man and farm animals but also from dogs and cats. They produce typical histological lesions called 'attaching and effacing' lesions. Both plasmid ... [more ▼]

Enteropathogenic Escherichia coli (EPEC) are isolated from man and farm animals but also from dogs and cats. They produce typical histological lesions called 'attaching and effacing' lesions. Both plasmid and chromosomal elements are involved in the pathogenesis of EPEC infection. The presence of these genetic elements was investigated in 14 dog and three cat EPEC isolates. A bfpA-related gene was detected in five of the 17 isolates in association with high molecular weight plasmids, and a locus of enterocyte effacement (LEE) was present in all isolates. The LEE was inserted in the selC region in only 12% of the isolates. The eae, tir, espA and espB genes were analyzed by multiplex PCR. The results indicated the presence of those genes in the tested isolates with heterogeneity in the gene subtypes present: eae gamma-tir alpha-espA alpha-espB alpha (65%), eae beta-tir beta-espA beta-espB beta (29%), eae alpha-tir alpha-espA alpha-espB alpha (6%). Moreover, the espD gene was also present in dog and cat EPEC. The DEPEC and CEPEC form a heterogeneous group and five of them are closely related to human EPEC. [less ▲]

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See detailPsychrophilic Enzymes: Revisiting the Thermodynamic Parameters of Activation May Explain Local Flexibility
Lonhienne, T.; Gerday, Charles ULg; Feller, Georges ULg

in Biochimica et Biophysica Acta (2000), 1543(1), 1-10

Basic theoretical and practical aspects of activation parameters are briefly reviewed in the context of cold-adaptation. In order to reduce the error impact inherent to the transition state theory on the ... [more ▼]

Basic theoretical and practical aspects of activation parameters are briefly reviewed in the context of cold-adaptation. In order to reduce the error impact inherent to the transition state theory on the absolute values of the free energy (DeltaG(#)), enthalpy (DeltaH(#)) and entropy (DeltaS(#)) of activation, it is proposed to compare the variation of these parameters between psychrophilic and mesophilic enzymes, namely Delta(DeltaG(#))(p-m), Delta(DeltaH(#))(p-m) and Delta(DeltaS(#))(p-m). Calculation of these parameters from the available literature shows that the main adaptation of psychrophilic enzymes lies in a significant decrease of DeltaH(#), therefore leading to a higher k(cat), especially at low temperatures. Moreover, in all cases including cold-blooded animals, DeltaS(#) exerts an opposite and negative effect on the gain in k(cat). It is argued that the magnitude of this counter-effect of DeltaS(#) can be reduced by keeping some stable domains, while increasing the flexibility of the structures required to improve catalysis at low temperature, as demonstrated in several cold-active enzymes. This enthalpic-entropic balance provides a new approach explaining the two types of conformational stability detected by recent microcalorimetric experiments on psychrophilic enzymes. [less ▲]

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See detailAdénomes cliniquement non fonctionnels et gonadotropes
Beckers, Albert ULg

Scientific conference (2000, November 30)

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See detailAdsorption of an aqueous mixture of surfactants on silica
Thibaut, Anne; Misselyn-Bauduin, Anne-Marie; Grandjean, Jean ULg et al

in Langmuir (2000), 16(24), 9192-9198

The behavior of mixtures of a nonionic surfactant, pentaethylene glycol monodecyl ether (C10E5), and an anionic surfactant, sodium dodecyl sulfate (SDS), was studied at the water−silica interface. In ... [more ▼]

The behavior of mixtures of a nonionic surfactant, pentaethylene glycol monodecyl ether (C10E5), and an anionic surfactant, sodium dodecyl sulfate (SDS), was studied at the water−silica interface. In contrast to the C10E5 surfactant, which is adsorbed at the silica−water interface at pH 6, the adsorption of the anionic surfactant is not observed. In the presence of SDS, the adsorption of C10E5 is severely restricted as result of the formation of mixed micelles in solution. This behavior is consistent with the adsorption free energy, ΔGads, of C10E5 and the free energy of mixed micellization, ΔGmix.mic, of SDS and C10E5. Flow microcalorimetry (FMC) results showed that the addition of C10E5/SDS mixtures to silica precoated with C10E5 resulted in the release of the preadsorbed nonionic surfactant; the whole phenomenon is exothermic. Combination of calorimetric data (FMC and isothermal titration calorimetry), self-diffusion coefficients, and the regular solution theory led to the conclusion that the main driving force for the C10E5 desorption was the formation of mixed micelles by unimeric SDS and the released nonionic surfactant. [less ▲]

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See detailLa valorisation de la betterave fourragère dans l'alimentation de la vache laitière et du bétail à l'engraissement
Beckers, Yves ULg

Conference given outside the academic context (2000)

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See detailPrevention of neonatal group B streptococcal disease in Belgium: hospital policy, obstetricians' practice and laboratory processing
MELIN, Pierrette ULg; Schmitz, Myriam; Heinrichs, I. et al

Poster (2000, November 25)

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See detailLe vieillissement cognitif: Au-delà des facteurs généraux
Hupet, Michel; Feyereisen, Pierre; Schelstraete, Marie-Anne et al

in Cognitiques = Cognitics (2000, November 25), 4

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See detailExploration de l’effet de simple exposition dans le vieillissement
Willems, Sylvie ULg; Adam, Stéphane ULg; Van der Linden, Martial ULg

in Revue de Neuropsychologie (2000, November 25), 9

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See detailExploration de l'effet de simple exposition dans le vieillissement
Willems, Sylvie ULg; Adam, Stéphane ULg; Van der Linden, Martial

in Cognitiques = Cognitics (2000, November 24), 4

La simple exposition répétée à un stimulus conduit les sujets à préférer ce stimulus par rapport à des stimuli non présentés. Nous avons exploré cet effet chez 22 sujets âgés (de 60 à 70 ans) et 22 sujets ... [more ▼]

La simple exposition répétée à un stimulus conduit les sujets à préférer ce stimulus par rapport à des stimuli non présentés. Nous avons exploré cet effet chez 22 sujets âgés (de 60 à 70 ans) et 22 sujets jeunes (de 20 à 30 ans) appariés quant au niveau d’éducation. Dans une première phase, on demande aux sujets d'estimer l'âge de 15 visages-cibles, non familiers. Ces 15 visages sont présentés durant 15 msec., cinq fois dans un ordre aléatoire. Ensuite, on présente aux sujets 15 paires de visages (un visage-cible et un visage non présenté) et on leur demande de choisir le visage qu'ils préfèrent. Enfin, un test de reconnaissance leur est administré. En ce qui concerne le jugement de préférence, les sujets âgés et les sujets jeunes choisissent préférentiellement le visage exposé antérieurement, avec une performance supérieure au hasard. De plus, la performance des deux groupes ne diffère pas significativement. Par contre, la performance des sujets âgés en reconnaissance est significativement inférieure à celle des sujets jeunes. En conclusion, ces résultats indiquent que les processus sous-tendant l'effet de simple exposition sont préservés par le vieillissement en dépit d'une reconnaissance explicite déficitaire. [less ▲]

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See detailAmorçage dans le vieillissement normal: Utilisation d’une approche permettant de réduire l’effet de contamination de la mémoire explicite
Adam, Stéphane ULg; Gouvars, Sébastien; Van der Linden, Martial

in Cognitiques = Cognitics (2000, November 24), 4

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See detailComment évaluer des compétences en classe d'histoire ?
Jadoulle, Jean-Louis ULg; Bouhon, Mathieu; Dambroise, Catherine

in PAQUAY, Léopold (Ed.) L'évalution des compétences chez l'apprenant. Pratiques, méthodes et fondements (2000, November 22)

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See detailLa vidéogrammétrie en milieux hostiles
Roose, Stéphane ULg; Henrist, Marc; Sarti, Bruno et al

in Actes du premier colloque francophone SFO-CMOI: Méthodes et techniques optiques pour l’industrie (2000, November 21)

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See detailResistance of Isolated Pulmonary Mitochondria During in Vitro Anoxia/Reoxygenation
Willet, K.; Detry, Olivier ULg; Sluse, Francis ULg

in Biochimica et Biophysica Acta (2000), 1460(2-3), 346-52

The aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh ... [more ▼]

The aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh pig lungs flushed with Euro-Collins solution. Mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5-45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of in vitro incubation in order to follow the in vitro aging of mitochondria during respiratory assays. Respiratory parameters measured after anoxia/reoxygenation did not show any oxidative phosphorylation dysfunction, indicating a high resistance of pulmonary mitochondria to in vitro anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage observed after in vivo ischemia (K. Willet et al., Transplantation 69 (2000) 582) but are a target of others cellular injuries leading to mitochondrial dysfunction in vivo. [less ▲]

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See detailA quinolinone derivative activating ATP-sensitive K+ channels
Becker, B.; Antoine, M. H.; Nguyen, Q. A. et al

Poster (2000, November 18)

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See detailPharmacological evaluation of 4,6-disubstituted 2,2-dimethylchromans structurally related to the KATP channel opener cromakalim
Sebille, S.; Becker, B.; Antoine, M. H. et al

Poster (2000, November 18)

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See detailHeart rate decrease induced by BPDZ 259, a diazoxide analogue
Damas, J.; Pirotte, Bernard ULg

Poster (2000, November 18)

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See detailUrinary excretion of high Mr PEG (>4000 Da) in rats
Nollevaux, G; Loret, S; Barakat, I et al

Poster (2000, November 18)

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