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See detailDetermination of the Enantiomers of 3-Tert.-Butylamino-1,2-Propanediol by High-Performance Liquid Chromatography Using Mass Spectrometric Detection
Toussaint, B.; Streel, B.; Ceccato, Attilio ULg et al

in Journal of Chromatography. A (2000), 896(1-2), 201-7

The chiral synthesis of beta-blockers such as (S)-timolol requires a sensitive analytical method for the enantioseparation of its intermediate, 3-tert.-butylamino-1,2-propanediol, in the ng/ml range. The ... [more ▼]

The chiral synthesis of beta-blockers such as (S)-timolol requires a sensitive analytical method for the enantioseparation of its intermediate, 3-tert.-butylamino-1,2-propanediol, in the ng/ml range. The method developed is based on on-line normal-phase LC-MS-MS using a chiral stationary phase and an atmospheric pressure chemical ionization (APCI) interface. The MS detection of 3-tert.-butylamino-1,2-propanediol was first optimized with a pneumatically-assisted electrospray interface (ionspray). The APCI interface was then selected for LC-MS-MS because of the incompatibility of electrospray with n-hexane. The method was validated for both enantiomers in the 25-500 ng/ml concentration range. [less ▲]

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See detailQuantitative Analysis of N-Acetylcysteine and Its Pharmacopeial Impurities in a Pharmaceutical Formulation by Liquid Chromatography-Uv Detection-Mass Spectrometry
Toussaint, B.; Pitti, C.; Streel, B. et al

in Journal of Chromatography. A (2000), 896(1-2), 191-9

A new method for the simultaneous determination of N-acetylcysteine and its pharmacopeial impurities, cysteine, cystine, N,N'-diacetylcystine and N,S-diacetylcysteine in an effervescent tablet has been ... [more ▼]

A new method for the simultaneous determination of N-acetylcysteine and its pharmacopeial impurities, cysteine, cystine, N,N'-diacetylcystine and N,S-diacetylcysteine in an effervescent tablet has been developed. The method is based on on-line LC-UV-MS using a pneumatically-assisted electrospray interface (ionspray). The stability of the thiol moieties of the analytes was ensured by the acidic pH of the LC mobile phase. Quantitation of N-acetylcysteine was performed with UV detection to avoid ion-source overloading effect due to its higher concentration, whereas the impurities could be easily separated and quantified in MS. The method was validated in terms of stability, linearity, precision and accuracy. [less ▲]

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See detailLa détection des signaux financiers annonciateurs de faillite en contexte PME : une approche méthodologique spécifique
Van Caillie, Didier ULg

in AIREPME (Ed.) Actes du 5ème Congrès International Francophone sur la PME (2000, October 26)

This paper proposes and discusses a structured and coherent methodology allowing to build up early warning business failure prediction models that are really able to incorporate the specificites of the ... [more ▼]

This paper proposes and discusses a structured and coherent methodology allowing to build up early warning business failure prediction models that are really able to incorporate the specificites of the financial data published by SMEs. [less ▲]

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See detailThe Extreme-Right in Western Europe : a Reevaluation
Martiniello, Marco ULg

Scientific conference (2000, October 25)

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See detailQuelques compositeurs belges à la Monnaie : le projet discographique du tricentenaire
Pirenne, Christophe ULg

Conference given outside the academic context (2000)

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See detailL’empreinte de Brecht en Belgique
Delhalle, Nancy ULg

Conference (2000, October 20)

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See detailGene activation by varicella-zoster virus IE4 protein requires its dimerization and involves both the arginine-rich sequence, the central part, and the carboxyl-terminal cysteine-rich region
Baudoux, Laurence; Defechereux, Patricia; Rentier, Bernard ULg et al

in Journal of Biological Chemistry (2000), 275(42), 32822-32831

Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for those of heterologous viruses. Since most transcription factors ... [more ▼]

Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for those of heterologous viruses. Since most transcription factors act as dimers, IE4 dimerization was studied using the mammalian two-hybrid system. Introduction of mutations in the IE4 open reading frame demonstrated that both the central region and the carboxyl-terminal cysteine-rich domain were important for efficient dimerization. Within the carboxyl-terminal domain, substitution of amino acids encompassing residues 443-447 totally abolished dimerization. Gene activation by IE4 was studied by transient transfection with an IE4 expression plasmid and a reporter gene under the control of either the human immunodeficiency virus, type 1, long terminal repeat or the VZV thymidine kinase promoter. Regions of IE4 important for dimerization were also shown to be crucial for transactivation. In addition, the arginine-rich domains Rb and Re of the amino-terminal region were also demonstrated to be important for transactivation, whereas the Ra domain as well as an acidic and bZIP-containing regions were shown to be dispensable for gene transactivation. A nucleocytoplasmic shuttling of IE4 has also been characterized, involving a nuclear localization signal identified within the Rb domain and a nuclear export mechanism partially depending on Crm-1. [less ▲]

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See detailL'hypertension artérielle systolique isolée
Krzesinski, Jean-Marie ULg

Conference (2000, October 18)

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See detailL'hypertension artérielle systolique isolée
Krzesinski, Jean-Marie ULg

Conference (2000, October 18)

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See detailColloque « Diversity in the City »
Martiniello, Marco ULg

Scientific conference (2000, October 17)

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See detailFree tropospheric CO, C2H6, and HCN above central Europe: Recent measurements from the Jungfraujoch station including the detection of elevated columns during 1998
Rinsland, C. P.; Mahieu, Emmanuel ULg; Zander, Rodolphe ULg et al

in Journal of Geophysical Research. Atmospheres (2000), 105(D19), 24235-24249

Time series of free tropospheric carbon monoxide (CO), ethane (C2H6), and hydrogen cyanide (HCN) column abundances have been derived from observations at the International Scientific Station of the ... [more ▼]

Time series of free tropospheric carbon monoxide (CO), ethane (C2H6), and hydrogen cyanide (HCN) column abundances have been derived from observations at the International Scientific Station of the Jungfraujoch (ISSJ) at 3.58-km altitude in the Swiss Alps (latitude 46.55 degreesN, 7.98 degreesE longitude). The free troposphere was assumed to extend from 3.58 to 11 km altitude, and the related columns were derived for all three molecules from high spectral resolution infrared solar spectra recorded between January 1995 and October 1999. The three molecules show distinct seasonal cycles with maxima during winter for CO and C2H6, and during spring for HCN. These seasonal changes are superimposed on interannual variations. The tropospheric columns of all three molecules were elevated during 1998. Increases were most pronounced for HCN with enhanced values throughout the year, up to a factor of 2 in January 1998 when compared to averages of the other years. The increased tropospheric columns coincide with the period of widespread wildfires during the strong El Nino warm phase of 1997-1998. The emission enhancements above ISSJ are less pronounced, and they peaked after the increases measured above Mauna Loa (19.55 degreesN, 155.6 degreesW). Tropospheric trends for CO, C2H6, and HCN of (2.40 +/- 0.49), (0.47 +/- 0.64), and (7.00 +/- 1.61)% yr(-1)(1 sigma) were derived for January 1995 to October 1999. However, if 1998 measurements are excluded from the fit, CO and HCN trends that are not statistically significant, and a statistically significant decrease in the C2H6 tropospheric column, are inferred. Comparisons of the infrared CO columns with CO in situ surface measurements suggest that the CO free tropospheric vertical Volume mixing ratio profile generally decreases with altitude throughout the year. [less ▲]

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See detailL'écobilan de l'exploitation agricole
Debouche, Charles ULg

Scientific conference (2000, October 16)

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See detailModeled and empirical approaches for retrieving columnar water vapor from solar transmittance measurements in the 0.72, 0.82, and 0.94 μm absorption bands
Ingold, T.; Schmid, B.; Mätzler, C. et al

in Journal of Geophysical Research (2000), 105(D19), 2432724343

A Sun photometer (18 channels between 300 and 1024 nm) has been used for measuring the columnar content of atmospheric water vapor (CWV) by solar transmittance measurements in absorption bands with ... [more ▼]

A Sun photometer (18 channels between 300 and 1024 nm) has been used for measuring the columnar content of atmospheric water vapor (CWV) by solar transmittance measurements in absorption bands with channels centered at 719, 817, and 946 nm. The observable is the band-weighted transmittance function defined by the spectral absorption of water vapor and the spectral features of solar irradiance and system response. The transmittance function is approximated by a three-parameter model. Its parameters are determined from MODTRAN and LBLRTM simulations or empirical approaches using CWV data of a dual-channel microwave radiometer (MWR) or a Fourier transform spectrometer (FTS). Data acquired over a 2-year period during 1996–1998 at two different sites in Switzerland, Bern (560 m above sea level (asl)) and Jungfraujoch (3580 m asl) were compared to MWR, radiosonde (RS), and FTS retrievals. At the low-altitude station with an average CWV amount of 15 mm the LBLRTM approach (based on recently corrected line intensities) leads to negligible biases at 719 and 946 nm if compared to an average of MWR, RS, and GPS retrievals. However, at 817 nm an overestimate of 2.7 to 4.3 mm (18–29%) remains. At the high-altitude station with an average CWV amount of 1.4 mm the LBLRTM approaches overestimate the CWV by 1.0, 1.4, and 0.1 mm (58, 76, and 3%) at 719, 817, and 946 nm, compared to the FTS instrument. At the low-altitude station, CWV estimates, based on empirical approaches, agree with the MWR within 0.4 mm (2.5% of the mean); at the high-altitude site with a factor of 10 less water vapor the agreement of the SPM with the FTS is 0.0 to 0.2 mm (1 to 9% of the mean CWV there). Sensitivity analyses show that for the conditions met at the two stations with CWV ranging from 0.2 to 30 mm, the retrieval errors are smallest if the 946 nm channel is used. [less ▲]

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See detailAn Active Layered Multicast Adaptation Protocol
Yamamoto, Lidia; Leduc, Guy ULg

in Lecture Notes in Computer Science (2000, October 16), 1942

We describe an active application in the field of multicast congestion control for real-time traffic. Our Active Layered Multicast Adaptation Protocol is a layered multicast congestion control scheme ... [more ▼]

We describe an active application in the field of multicast congestion control for real-time traffic. Our Active Layered Multicast Adaptation Protocol is a layered multicast congestion control scheme built on top of an Active Network infrastructure. It benefits from router support in order to obtain information about resources available and to perform the adaptation tasks at the places where shortage of resources occur. It supports heterogeneous receivers through the combination of layered multicast transmission with selective filtering and pruning of layers within the active nodes. Market-based resource management ideas are applied to achieve a resource utilisation level that represents an equilibrium between the user goals and the node operator goals. Our simulation results show that the protocol is feasible and provides adequate reactions to short term and persistent congestion, while keeping the amount of state and processing in the active nodes limited. [less ▲]

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See detailNuclear Factor-Kappa B, Cancer, and Apoptosis
Bours, Vincent ULg; Bentires-Alj, M.; Hellin, A. C. et al

in Biochemical Pharmacology (2000), 60(8), 1085-9

The role of nuclear factor (NF)-kappa B in the regulation of apoptosis in normal and cancer cells has been extensively studied in recent years. Constitutive NF-kappa B activity in B lymphocytes as well as ... [more ▼]

The role of nuclear factor (NF)-kappa B in the regulation of apoptosis in normal and cancer cells has been extensively studied in recent years. Constitutive NF-kappa B activity in B lymphocytes as well as in Hodgkin's disease and breast cancer cells protects these cells against apoptosis. It has also been reported that NF-kappa B activation by tumor necrosis factor (TNF)-alpha, chemotherapeutic drugs, or ionizing radiations can protect several cell types against apoptosis, suggesting that NF-kappa B could participate in resistance to cancer treatment. These observations were explained by the regulation of antiapoptotic gene expression by NF-kappa B. However, in our experience, inhibition of NF-kappa B activity in several cancer cell lines has a very variable effect on cell mortality, depending on the cell type, the stimulus, and the level of NF-kappa B inhibition. Moreover, in some experimental systems, NF-kappa B activation is required for the onset of apoptosis. Therefore, it is likely that the NF-kappa B antiapoptotic role in response to chemotherapy is cell type- and signal-dependent and that the level of NF-kappa B inhibition is important. These issues will have to be carefully investigated before considering NF-kappa B as a target for genetic or pharmacological anticancer therapies. [less ▲]

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See detailA propos de la contemporanéité de l'hôpital: histoire d'un devenir sans histoire
Havelange, Carl ULg

Conference given outside the academic context (2000)

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See detailLocal buckling in case of fire
Franssen, Jean-Marc ULg; Talamona, Didier

Conference (2000, October 13)

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See detailL'institution du regard. De l'invention de la perspective à celle de la photographie
Havelange, Carl ULg

Scientific conference (2000, October 10)

Detailed reference viewed: 8 (1 ULg)