References of "1999"
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See detailApplication of sucrose synthase in the synthesis of nucleotide sugars and saccharides
Zervosen, Astrid ULiege; Elling, Lothar

in Bucke, Christopher (Ed.) Carbohydrate Biotechnology Protocols (1999)

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See detailEffects of Nimesulide and Sodium Diclofenac on Interleukin-6, Interleukin-8, Proteoglycans and Prostaglandin E2 Production by Human Articular Chondrocytes in Vitro
Henrotin, Yves ULiege; Labasse, A. H.; Simonis, P. E. et al

in Clinical and Experimental Rheumatology (1999), 17(2), 151-60

OBJECTIVES: The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG ... [more ▼]

OBJECTIVES: The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG), prostaglandin E2 (PGE2) and cytokines (IL-6 and IL-8) by human articular chondrocytes in vitro. METHODS: Enzymatically isolated chondrocytes were cultured under constant agitation in a well defined culture medium. Specific radioimmunoassays were used to quantify PG and PGE2 production. Cytokine production (IL-6 and IL-8) was assayed by enzyme amplified sensitivity immunoassays (EASIAs). RESULTS: At a concentration of 3 micrograms/ml, nimesulide did not affect the PG production by chondrocytes. This concentration was superior to the highest level of nimesulide found in the synovial fluid of patients with rheumatoid arthritis 3 hours after the last oral administration of nimesulide (100 mg twice daily for 7 days). At 6 micrograms/ml a significant reduction in the PG content was obtained in the cellular phase in 5 out of the 8 cultures investigated. No similar effect was observed in the culture supernatants. Above this concentration nimesulide inhibited PG production in a dose-dependent manner. At concentrations ranging from 0.005 to 1 microgram/ml diclofenac did not significantly alter PG production. At therapeutic concentrations PGE2 production was totally inhibited by nimesulide, thus suggesting that PG inhibition is not linked to PGE2 production. Nimesulide inhibited PGE2 production by unstimulated (IC50 = 6 ng/ml) and IL-1 beta-stimulated (IC50 = 6.9 ng/ml) chondrocytes. At these concentrations, PGE2 production was fully inhibited by diclofenac. Furthermore, both nimesulide and diclofenac at therapeutic concentrations significantly decreased spontaneous and IL-1 beta-stimulated IL-6 production by human chondrocytes, but did not modify IL-8 production. CONCLUSION: From the results of this study we conclude that nimesulide and diclofenac at therapeutic concentrations are potent inhibitors of PGE2 and IL-6 production while they do not modify proteoglycan or IL-8 production. [less ▲]

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See detailThe use of different dual X-ray absorptiometry brands in a multicenter clinical trial
Slosman, DO; Provedini, DM; Meunier, PJ et al

in Journal of Clinical Densitometry : The Official Journal of the International Society for Clinical Densitometry (1999), 2

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See detailFine-Mapping of Quantitative Trait Loci by Identity by Descent in Outbred Populations: Application to Milk Production in Dairy Cattle
Riquet, J.; Coppieters, Wouter ULiege; Cambisano, Nadine ULiege et al

in Proceedings of the National Academy of Sciences of the United States of America (1999), 96(16), 9252-9257

We previously mapped a quantitative trait locus (QTL) affecting milk production to bovine chromosome 14. To refine the map position of this QTL, we have increased the density of the genetic map of ... [more ▼]

We previously mapped a quantitative trait locus (QTL) affecting milk production to bovine chromosome 14. To refine the map position of this QTL, we have increased the density of the genetic map of BTA14q11-16 by addition of nine microsatellites and three single nucleotide polymorphisms. Fine-mapping of the QTL was accomplished by a two-tiered approach. In the first phase, we identified seven sires heterozygous "Qq" for the QTL by marker-assisted segregation analysis in a Holstein-Friesian pedigree comprising 1,158 individuals. In a second phase, we genotyped the seven selected sires for the newly developed high-density marker map and searched for a shared haplotype flanking an hypothetical, identical-by-descent QTL allele with large substitution effect. The seven chromosomes increasing milk fat percentage were indeed shown to carry a common chromosome segment with an estimated size of 5 cM predicted to contain the studied QTL. The same haplotype was shown to be associated with increased fat percentage in the general population as well, providing additional support in favor of the location of the QTL within the corresponding interval. [less ▲]

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See detailF. Guicciardini, Compendio della «Cronica» di Froissart.
Moreno, Paola ULiege

Book published by Commissione per i testi di Lingua (1999)

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See detailProbabilistic design of power-system special stability controls
Wehenkel, Louis ULiege; Lebrevelec, Cyril; Trotignon, Marc et al

in Control Engineering Practice (1999), 7(2), 183-194

A probabilistic approach to the design of power-system special stability controls is presented here. Using Monte-Carlo simulations, it takes into account all the potential causes of blackouts, slow and ... [more ▼]

A probabilistic approach to the design of power-system special stability controls is presented here. Using Monte-Carlo simulations, it takes into account all the potential causes of blackouts, slow and fast dynamics, and modeling uncertainties. A large number of scenarios are simulated in parallel by time-domain numerical integration, and the relevant parameters of the resulting system trajectories are stored in a database. Data-mining tools are used to identify the most important system weaknesses and possible improvements. The approach is tested on a large-scale study on the SouthÐEastern part of the extra-high-voltage system of Electricité de France. [less ▲]

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See detailPeriodontitis as potential risk factor for coronary heart disease
Geerts, Sabine ULiege; Nys, Monique; Legrand, Victor et al

Poster (1999)

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See detailBreeding for "low-gossypol seed and high-gossypol plants" in upland cotton. Analysis of tri-species hybrids and backcross progenies using AFLPs and mapped RFLPs.
Vroh Bi, I.javascript:void(0); Maquet, A.; Baudoin, Jean-Pierre ULiege et al

in Theoretical and Applied Genetics (1999), 99(7-8),

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See detailLes agents antiplaquettaires en chirurgie vasculaire périphérique
VAN DAMME, Hendrik ULiege; DAVID, Jean-Louis ULiege; Limet, Raymond ULiege

in Revue Médicale de Liège (1999), 54(2), 109-17

Prescription of platelet inhibitors after arterial surgery is common use. The major concern of the vascular surgeon is to maintain patency of arterial reconstructions. Major causes of graft failure or ... [more ▼]

Prescription of platelet inhibitors after arterial surgery is common use. The major concern of the vascular surgeon is to maintain patency of arterial reconstructions. Major causes of graft failure or arterial thrombosis are the non-thromboresistant nature of the grafts and of the endarterectomised or balloon-dilated surfaces, restenosis due to intimal hyperplasia and progression of atherosclerotic disease in in- or outflow vessels. Platelet adhesion and intimal injury are the primary causes in both processes of graft thrombosis and intimal hyperplasia. To understand how antiplatelet drugs can interfere with these processes, a brief review of platelet function, and of the main platelet inhibitors (aspirin, dypiridamole, ticlopidine) is given. The pathophysiology of intimal hyperplasia is discussed. From clinical trials of peripheral vascular surgery or percutaneous transluminal angioplasty with or without periprocedural antiplatelet therapy, it appears that platelet inhibitors reduce early failure rate by 50% (thrombosis rate at one year reduced from 40 to 20%). There is also evidence that antiplatelet drugs allow to slow down the progression of the atherosclerotic degenerative process in the outflow vessels and in other vascular territories. For the polyvascular patients with charged passed history, platelet inhibitors reduce the risk of myocardial or cerebral infarction by 30% (secondary prevention). Today, there is a general consensus that antiplatelet drugs, started the day before the procedure, are beneficial for early and late patency of peripheral vascular reconstructions (carotid endarterectomy, infrainguinal bypass grafts or endovascular procedures). [less ▲]

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See detailHabitat de reproduction des poissons et processus géomorphologiques dans les rivières à fond caillouteux: essai de synthèse et applications à quelques rivières du bassin de la Meuse.
Parkison, D.; Petit, François ULiege; Perpinien, G. et al

in Bulletin de la Société Géographique de Liège (1999)

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See detailIn vitro models for the study of cartilage damage and repair
Henrotin, Yves ULiege; Reginster, Jean-Yves ULiege

in Reginster, Jean-Yves; Pelletier, J-P; Martel-Pelletier, J (Eds.) et al Osteoarthritis: Clinical and experimental aspects (1999)

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See detailDynamics of rRNA transcripts within the nucleolus as revealed with confocal and electron microscopy
Thiry, Marc ULiege; O'Donohue, Marie-Christine; Kaplan, Hervé et al

Poster (1999)

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See detailRéférent hospitalier pour la continuité des soins
de Froidmont, C; Gosset, Christiane ULiege

Conference (1999)

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See detailUDP-N-Acetyl-alpha-D-glucosamine as acceptor substrate of beta-1,4-galactosyltransferase. Enzymatic synthesis of UDP-N-acetyllactosamine.
Elling, Lothar; Zervosen, Astrid ULiege; Gallego, Ricardo Gallego et al

in Glycoconjugate Journal (1999), 16(7), 327-36

The capacity of UDP-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor substrate for beta-1,4-galactosyltransferase (beta4GalT1, EC 2.4.1.38) from human and bovine milk and for recombinant ... [more ▼]

The capacity of UDP-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor substrate for beta-1,4-galactosyltransferase (beta4GalT1, EC 2.4.1.38) from human and bovine milk and for recombinant human beta4GalT1, expressed in Saccharomyces cerevisiae, was evaluated. It turned out that each of the enzymes is capable to transfer Gal from UDP-alpha-D-galactose (UDP-Gal) to UDP-GlcNAc, affording Gal(beta1-4)GlcNAc(alpha1-UDP (UDP-LacNAc). Using beta4GalT1 from human milk, a preparative enzymatic synthesis of UDP-LacNAc was carried out, and the product was characterized by fast-atom bombardment mass spectrometry and 1H and 13C NMR spectroscopy. Studies with all three beta4GalTs in the presence of alpha-lactalbumin showed that the UDP-LacNAc synthesis is inhibited and that UDP-alpha-D-glucose is not an acceptor substrate. This is the first reported synthesis of a nucleotide-activated disaccharide, employing a Leloir glycosyltransferase with a nucleotide-activated monosaccharide as acceptor substrate. Interestingly, in these studies beta4GalT1 accepts an alpha-glycosidated GlcNAc derivative. The results imply that beta4GalT1 may be responsible for the biosynthesis of UDP-LacNAc, previously isolated from human milk. [less ▲]

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See detailGrass sickness: a Belgian reality
Christmann, U; Cassart, Dominique ULiege; Gabriel, Annick ULiege et al

in Proceedings of the 38th Annual Congress of the British Equine Veterinary Association (BEVA) (1999)

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