Reproducibility of measurements of potential doubling time of tumour cells in the multicentre National Cancer Institute protocol

in The importance of cellular kinetics in human cancer (1999)

We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose ... [more ▼]

We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose was to understand and minimize the variation within the measurement. A total of 102 specimens were selected at random from patients entered into the trial. Samples were prepared, stained, run and analysed in each centre and a single set of data analysed by all three centres. Analysis of the disc data set revealed that the measurement of labelling index (LI) was robust and reproducible. The estimation of duration of S-phase ( Ts) was subject to errors of profile interpretation, particularly DNA ploidy status, and analysis. The LI dominated the variation in Tpot such that the level of final agreement, after removal of outliers and ploidy agreement, reached correlation coefficients of 0.9. The sample data showed poor agreement within each of the components of the measurement. There was some improvement when ploidy was in agreement, but correlation coefficients failed to exceed values of 0.5 for Tpot. The data suggest that observer-associated analysis of Ts and tissue processing and tumour heterogeneity were the major causes of variability in the Tpot measurement. The first two aspects can be standardized and minimized, but heterogeneity will remain a problem with biopsy techniques. [less ▲]

The Devonian-Carboniferous Boundary in South Africa and the age of the earliest episode of the Dwyka Glaciation : new palynological result.

in Episodes (1999), 22(1), 41-44

Dynamics of rRNA transcripts within the nucleolus as revealed with confocal and electron microscopy

Poster (1999)

Référent hospitalier pour la continuité des soins

Conference (1999)

Grass sickness: a Belgian reality

in Proceedings of the 38th Annual Congress of the British Equine Veterinary Association (BEVA) (1999)

Le traitement des ectasies anevrysmales de l'aorte ascendante par l'intervention de Robicsek

in Revue Médicale de Liège (1999), 54(11), 886-8

The technique and the results of the external contention of the dilated ascending aorta by the application of an external Dacron graft are reported. This technique, without replacing the usual graft ... [more ▼]

The technique and the results of the external contention of the dilated ascending aorta by the application of an external Dacron graft are reported. This technique, without replacing the usual graft interposition, can efficiently and expeditiously treat borderline dilatation of the ascending aorta, as frequently encountered in aortic valve disease. If the sinotubular junction is not dilated, this technique may stop the aneurysmal evolution of the ascending aorta. [less ▲]

Oral status and nutrition in the institutionalized elderly.

in Journal of Dentistry (1999), 27(6), 443-8

OBJECTIVES: To evaluate, in an elderly population, whether poor oral status might be a contributing factor to the development of undernutrition and might be associated with less eating pleasure, more ... [more ▼]

OBJECTIVES: To evaluate, in an elderly population, whether poor oral status might be a contributing factor to the development of undernutrition and might be associated with less eating pleasure, more subjective eating difficulty and increased mashed food consumption. METHODS: An oral examination and an evaluation of masticatory capacity were performed on 120 institutionalized elderly subjects. The nutritional assessment included serum albumin concentration, the Mini Nutritional Assessment and a questionnaire on eating habits. RESULTS: Edentulous subjects without dentures or with only one complete denture had significantly lower MNA scores than edentulous subjects with two complete dentures (p < 0.05). Edentulous subjects with two complete dentures more frequently reported taking pleasure from eating (p = 0.05), and had less frequent difficulties with hard foods (p = 0.01) than edentulous subjects without dentures or with only one complete denture. Mashed food consumption (p < 0.01) was also reported more frequently in edentulous subjects without dentures or with only one complete denture. Subjects with two complete dentures had similar or better MNA scores as dentate subjects with relatively few remaining teeth (10.4 +/- 7.8 teeth). About half of the subjects (53%) could not perform the masticatory test. These subjects had lower MNA scores (p = 0.001) and a larger proportion ate mashed food (p < 0.001) compared to those who were able to perform the test. CONCLUSIONS: Poor oral status (edentulous without dentures or with only one complete denture) increased difficulty in eating hard foods, increased mashed food consumption and decreased eating pleasure. It seemed also to put institutionalized subjects at higher risk of undernutrition. [less ▲]

Présentation des relations de l'Unité d'Economie générale avec l'Asie

Speech (1999)

Pour une politique migratoire humaine et réaliste en Belgique, dans l'Europe de demain

in Annales de Droit de Louvain (1999), 59(3), 223-244

Suspected environmental zinc poisoning in a foal

in Journal of Equine Veterinary Science (1999), 19