References of "1999"
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See detailMémoire de travail et lobes frontaux
Collette, Fabienne ULg; Andrès; Van der Linden, Martial ULg

in Van der Linden, Martial; Le Gall, Didier; Seron, Xavier (Eds.) et al Neuropsychologie des lobes frontaux (1999)

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See detailCapture for coorbital moons
Champenois, Sylvain; Henrard, Jacques; Jancart, Sylvie ULg

Poster (1999)

See detailThe free movement of persons living with HIV/AIDS
Carlier, Jean-Yves ULg

Book published by OPOCE (1999)

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See detailIntérêt d'une procédure de rappel indicé à 48 items dans le diagnostic précoce de la maladie d'Alzheimer
Ivanoiu, Adrian; Adam, Stéphane ULg; Béchet, Sophie et al

in Revue Neurologique (1999), 155

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See detailReproducibility of measurements of potential doubling time of tumour cells in the multicentre National Cancer Institute protocol
COUCKE, Philippe ULg; Paschoud, N; Pavy, J-J et al

in British Journal of Cancer (1999)

We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose ... [more ▼]

We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in the National Cancer Institute (NCI) protocol T92-0045. The primary purpose was to understand and minimize the variation within the measurement. A total of 102 specimens were selected at random from patients entered into the trial. Samples were prepared, stained, run and analysed in each centre and a single set of data analysed by all three centres. Analysis of the disc data set revealed that the measurement of labelling index (LI) was robust and reproducible. The estimation of duration of S-phase ( Ts) was subject to errors of profile interpretation, particularly DNA ploidy status, and analysis. The LI dominated the variation in Tpot such that the level of final agreement, after removal of outliers and ploidy agreement, reached correlation coefficients of 0.9. The sample data showed poor agreement within each of the components of the measurement. There was some improvement when ploidy was in agreement, but correlation coefficients failed to exceed values of 0.5 for Tpot. The data suggest that observer-associated analysis of Ts and tissue processing and tumour heterogeneity were the major causes of variability in the Tpot measurement. The first two aspects can be standardized and minimized, but heterogeneity will remain a problem with biopsy techniques. [less ▲]

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See detailElectron-microscopic tomography of silver-stained interphase and metaphase nucleolar organizer regions
Cheutin, T; O'Donohue, M-F; Kaplan, H et al

Poster (1999)

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See detailEtude d'un réseau cyclable à Liège
Hanocq, Philippe ULg; Piron, Frédéric; Occhiutto, Rita

in Croughs, Roger; Jacobs, Chantal; Guillaume, Michèle (Eds.) Communes cyclistes ? Ca existe ! ... Quelsques pistes pour promouvoir l'usage du vélo dans votre commune (1999)

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See detailInternational laws: collecting, transporting and ownership of fossils - Belgium.
Gerrienne, Philippe ULg

in Jones, T.P.; Rowe, N.P. (Eds.) Fossil Plants and Spores: Modern Techniques. (1999)

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See detailThe relationship between diversity profiles, evenness and species richness based on partial ordering
Rousseau, R; Van Hecke, P; Nijssen, D et al

in Environmental and Ecological Statistics (1999), 6(2), 211-223

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See detailSimultaneous Determination of Deoxyribonucleoside in the Presence of Ribonucleoside Triphosphates in Human Carcinoma Cells by High-Performance Liquid Chromatography
Decoster, L-A; Chen, X; Lejeune, F et al

in Analytical Biochemistry (1999), 270

Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in cells by HPLC is an analytical challenge since the concentration of dNTP present in mammalian cells is several orders ... [more ▼]

Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in cells by HPLC is an analytical challenge since the concentration of dNTP present in mammalian cells is several orders of magnitude lower than the corresponding NTP. Hence, the quantitation of dNTP in cells is generally performed after selective oxidation or removal of the major NTP. The procedures reported so far are lengthy and cumbersome and do not enable the simultaneous determination of NTP. We report the development of a simple, direct HPLC method for the simultaneous determination of dNTP and NTP in colon carcinoma WiDr cell extracts using a stepwise gradient elution ion-pairingHPLCwith uv detection at 260 nm and with a minimal chemical manipulation of cells. Exponentially growing WiDr cells were harvested by centrifugation, rinsed with phosphate- buffered saline, and carefully counted. The pellets were suspended in a known volume of ice-cold water and deproteinized with an equal volume of 6% trichloroacetic acid. The acid cell extracts (corresponding to 2.53 106 cells/100 ml) were centrifuged at 13,000g for 10 min at 4°C. The resulting supernatants were stored at 280°C prior to analysis. Aliquots (100 ml) were neutralized with 4.3 ml saturated Na2CO3 solution prior the injection of 40 ml onto the HPLC column (injection speed 250 ml/min). Chromatographic separations were performed using two Symmetry C18 3.5-mm (2 3 3.9 3 150 mm) columns (Waters), connected in series equipped with a Sentry guard column (3.9 3 20 mm i.d.) filled with the same packing material. The HPLC columns were kept at 30°C. The mobile phase was delivered at a flow rate of 0.5 ml/min, with the following stepwise gradient elution program: % solvent A/solvent B, 100/0 at 0 min 3 100/0 at 1 min 3 36/64 at 5 min 3 31/69 at 90 min 3 31/69 at 105 min 3 0/100 at 106 min 3 0/100 at 120 min; 50/50 MeOH/solvent B from 121 to 130 min; 100% solvent A from 131 to 160 min. Solvent A contained 0.01 M KH2PO4, 0.01 M tetrabutylammonium chloride, and 0.25% MeOH and was adjusted to pH 7.0 (550 ml 10 N NaOH for 1 liter solvent A). Solvent B consisted of 0.1 MKH2PO4, 0.028Mtetrabutylammonium chloride, and 30% MeOH and was neutralized to pH 7.0 (1.4 ml 10 N NaOH for 1 liter solvent B). Even though dNTPs are minor components of cell extracts, satisfactory regression coefficients were obtained for their calibration curves (r2 > 0.99) established with the addition–calibration methods up to 120 pmol/40-ml injection. The applicability of the method was demonstrated by in vitro studies of the modulation of NTP and dNTP pools in WiDr colon carcinoma cell lines exposed to various pharmacological concentrations of cytostatic drugs (i.e., FMdC, IUdR, gemcitabine). In conclusion, this optimized, simplified, analytical method enables the simultaneous quantitation of NTP and dNTP and may represent a valuable tool for the detection of minute alterations of cellular dNTP/NTP pools induced by anticancer/ antiviral drugs and diseases. © 1999 Academic Press [less ▲]

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See detailTwo new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide.
Fanuel, L; Thamm, Iris ULg; Kostanjevecki, V et al

in Cellular and Molecular Life Sciences : CMLS (1999), 55(5), 812-8

Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to homogeneity and found to be homologous to the ... [more ▼]

Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to homogeneity and found to be homologous to the Dap protein produced by O. anthropi SCRC C1-38 (ATCC49237). The second enzyme, DmpA, exhibits a similar substrate profile when tested on p-nitroanilide derivatives of glycine and L/D-alanine, but the amounts produced by the Ochrobactrum strain were not sufficient to allow complete purification. Interestingly, the DmpA preparation also exhibited an L-aminopeptidase activity on the tripeptide L-Ala-Gly-Gly but it was not possible to be certain that the same protein was responsible for both p-nitroanilide and peptide hydrolysing activities. The gene encoding the DmpA protein was cloned and sequenced. The deduced protein sequence exhibits varying degrees of similarity with those corresponding to several open reading frames found in the genomes of other prokaryotic organisms, including Mycobacteria. None of these gene products has been isolated or characterised, but a tentative relationship can be proposed with the NylC amidase from Flavobacterium sp. K172. [less ▲]

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