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See detailTransglutaminase-Mediated Oligomerization of Galectin-3 Modulates Human Melanoma Cell Interactions with Laminin
van den Brule, F. A.; Liu, F. T.; Castronovo, Vincenzo ULg

in Cell Adhesion and Communication (1998), 5(6), 425-35

Tumor cell adhesion and migration to laminin are important events during invasion and metastatic spread. Galectin-3, a multifunctional member of the galectin family, binds specifically the poly-N ... [more ▼]

Tumor cell adhesion and migration to laminin are important events during invasion and metastatic spread. Galectin-3, a multifunctional member of the galectin family, binds specifically the poly-N-acetyllactosamine residues of laminin and has been implicated in tumor invasion and metastasis. Galectin-3 is multimerized by transglutaminase, an enzyme that catalyzes cross-linking between glutamine and other aminoacid residues. In this study, we examined the consequences of transglutaminase-mediated galectin-3 oligomerization on the interactions between cancer cells and laminin. We first demonstrated that human galectin-3 is cross-linked by guinea pig liver transglutaminase, forms oligomers, and incorporates the marker 5-(biotinamido) pentylamine. Expression of transglutaminase activity in the A375 and A2058 human melanoma cell extracts was revealed by its ability to induce galectin-3 oligomerization and 5-(biotinamido) pentylamine incorporation. Transglutaminase-treated galectin-3 did not affect adhesion or migration of the melanoma cells to laminin but consistently induced a significant increase of the percentage of cell spreading compared to the control (23.5 +/- 2.3%, vs. 10.6 +/- 1.9% at 180 min, p < 0.05), or to untreated galectin-3 or transglutaminase alone. Our study is the first demonstration that human galectin-3 is oligomerized by transglutaminase with, as a consequence, a specific effect of melanoma cell spreading on laminin. This phenomenon could be of significance in the modulation of cancer cell interactions with laminin during tumor invasion and metastasis. [less ▲]

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See detailSystème d'Information Multimédia de Gestion Environnementale des Communes
Hauglustaine, Jean-Marie ULg; Navez, J.-C.

Report (1998)

The principal goal of the study is to equip the small ones and common averages, of the computer tools of which they miss as regards management of the environment, and in particular in the following fields ... [more ▼]

The principal goal of the study is to equip the small ones and common averages, of the computer tools of which they miss as regards management of the environment, and in particular in the following fields: - the quality of the built medium, public spaces and the landscapes; - the consumption of energy of heating in the public buildings and the management of public lighting; - draining, the cleansing, the management of the cycle of water; - the waste harvest and processing. [less ▲]

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See detailSensus communis
Spinoy, Erik ULg

in De Vlaamse Gids (1998), 82(4), 13-15

This article is a critical reflection on the literary views held by the Dutch postwar poet Rutger Kopland.

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See detailAttachment but Not Penetration of Bovine Herpesvirus 1 Is Necessary to Induce Apoptosis in Target Cells
Hanon, Emmanuel; Meyer, Gilles; Vanderplasschen, Alain ULg et al

in Journal of Virology (1998), 72(9), 7638-41

Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in bovine peripheral blood mononuclear cells and B-lymphoma cells. Using a BHV-1 glycoprotein H null mutant, we have demonstrated that although ... [more ▼]

Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in bovine peripheral blood mononuclear cells and B-lymphoma cells. Using a BHV-1 glycoprotein H null mutant, we have demonstrated that although penetration of BHV-1 is not required, attachment of BHV-1 viral particles is essential for the induction of apoptosis. [less ▲]

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See detailMixed monolayers of gluten-emulsifiers at the air-water interface.
Balla, A.; Blecker, Christophe ULg; Razafindralambo, Hary ULg et al

Poster (1998, September)

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See detailSupramolecular organization of model liquid crystalline ionomers
Gohy, Jean-François; Jérôme, Robert ULg; Van den Bossche, Guy ULg et al

in Macromolecular Chemistry and Physics (1998), 199(9), 1791-1799

A mesogenic cation was associated with low molecular weight carboxylato- and sulfonato(se-mi)telechelic polymers. These compounds were prepared by ion-exchange of the quaternary ammonium group attached to ... [more ▼]

A mesogenic cation was associated with low molecular weight carboxylato- and sulfonato(se-mi)telechelic polymers. These compounds were prepared by ion-exchange of the quaternary ammonium group attached to the mesogen for the metal counterion of the halato(semi)telechelic polymer. The accordingly formed liquid crystalline halato(semi)telechelic polymers (LC H(S)TP's) were analyzed by differential scanning calorimetry (DSC), polarized-light optical microscopy (POM), and small angle X-ray scattering (SAXS). On the basis of these experimental results, a model for the supramolecular organization of the LC H(S)TP's is proposed. The final morphology results from the interplay of two competitive effects: the dipolar interaction of the ion-pairs known for multiplet formation and the propensity of the mesogenic moiety to form mesophases. The outcome of this competition depends on the mobility of the mesogenic counterion, i.e., on the strength of the dipolar interactions, the mobility of the polymer backbone and the mesogen/poly-mer ratio which is controlled by the molecular weight of the polymer. [less ▲]

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See detailExploration isocinétique de la relation tension longueur musculaire
Croisier, Jean-Louis ULg; Camus, Gérard; Ledent, M. et al

in Archives of Physiology & Biochemistry (1998, September), 106(Suppl B), 128

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See detailOcclusion dentaire : retentissement sur la force musculaire
Croisier, Jean-Louis ULg; Jalali, R.; Fernandez-Moreno, S. et al

in Archives of Physiology & Biochemistry (1998, September), 106(Suppl B), 154

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See detailLa relation force vitesse étudiée en isocinétisme
Croisier, Jean-Louis ULg; Camus, Gérard; Ledent, M. et al

in Archives of Physiology & Biochemistry (1998, September), 106(Suppl B), 70

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See detailModélisation de l'articulation fémoro patellaire et calcul des contraintes lors de la contraction du quadriceps
Krajewski, L.; Croisier, Jean-Louis ULg; Collette, C. et al

in Archives of Physiology & Biochemistry (1998, September), 106(Suppl B), 76

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See detailParacrine control of cytokine secretion by human endometrial epithelium
Geenen, Vincent ULg

Conference (1998, September)

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See detailInfections à Cytomegalovirus (CMV) chez les transplantés d’organes
Detry, Olivier ULg

in Virologics : la Virologie du Sida au Zona (1998)

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See detailAmphetamine-Induced Conditioned Activity and Sensitization: The Role of Habituation to the Test Context and the Involvement of Pavlovian Processes
Tirelli, Ezio ULg; Terry, P.

in Behavioural Pharmacology (1998, September), 9(5-6), 409

Behaviours associated with drug action can sometimes be elicited, in the absence of drug, by exposure to stimuli that were present during drug administration. Such a finding is usually interpreted as a ... [more ▼]

Behaviours associated with drug action can sometimes be elicited, in the absence of drug, by exposure to stimuli that were present during drug administration. Such a finding is usually interpreted as a conditioned drug effect. Often, however, the outcome could arise if drug administration in a particular environment retarded behavioural habituation to that environment. To test the 'habituation hypothesis' of conditioned drug effects, mice received 10 daily injections of d-amphetamine ('paired' group) or saline ('unpaired') in test boxes, and the converse injections in the colony room. Another group received saline in both environments. The apparatus and procedures yielded minimal habituation of behaviours (ambulation and rearing) over sessions. Only the paired group demonstrated behavioural sensitization, indicating environment-specific sensitization. The paired group also showed more ambulation and rearing than the others on the critical test of conditioning (saline injection in test box); moreover, their conditioning test scores were higher than those of the other groups on their first exposure to the test boxes, contradicting the habituation hypothesis. Further supporting the involvement of Pavlovian conditioning, levels of ambulation and rearing measured for 10 min before each injection increased in the paired group, relative to the unpaired groups, over successive pairing sessions. Tests controlling for differential handling/injection experience produced results consistent with those previously obtained. Together, the findings are incompatible with the habituation hypothesis, and further support the role of Pavlovian conditioning. [less ▲]

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See detailVolume of Structures : Application to Classical and Harmonic Structures
Samyn, Philippe; Latteur, Pierre ULg; Van Vooren, J.

(1998, September)

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