Myasthenia gravis without chronic GVHD after allogeneic bone marrow transplantation.
Baron, Frédéric ; Sadzot, Bernard ; Wang, François-Charles et al
in Bone Marrow Transplantation (1998), 22(2), 197-200
A 20-year-old man with aplastic anemia developed myasthenia gravis (MG) 7 months after bone marrow transplantation (BMT) from an HLA one locus-mismatched sister. Proximal muscle weakness (predominant in ... [more ▼]
A 20-year-old man with aplastic anemia developed myasthenia gravis (MG) 7 months after bone marrow transplantation (BMT) from an HLA one locus-mismatched sister. Proximal muscle weakness (predominant in the lower limbs) and dysphagia occurred without any other sign of graft-versus-host disease (GVHD), 1 month after cessation of immunosuppression with cyclosporine. The diagnosis of MG was based on clinical symptoms and on neurophysiologic investigations showing a significant increase of the Jitter in single-fiber electromyography and a significant decremental response during repetitive stimulation at slow rates, but antibodies against the acetylcholine receptor (AchRab) were negative. All clinical and neurophysiological signs normalized within 1 month of treatment with low-dose prednisolone and pyridostigmine, and the patient is perfectly well 1 year after cessation of all therapy. All cases of BMT-associated MG previously published are reviewed in comparison with ours. The originality of this new observation is that this case is the only one not associated with chronic GVHD and negative for AchRab. Alternatively, MG may have been the sole manifestation of chronic GVHD in this patient. [less ▲]Detailed reference viewed: 42 (2 ULg)
La comparution du vendeur devant le tribunal d'un Etat dans le cadre d'un litige déterminé n'emporte pas renonciation dans le chef de celui-ci à se prévaloir dans le cadre d'une instance différente de la clause attributive de juridiction contenue dans ses conditions générales au profit des tribunaux d'un autre Etat
in Revue de Droit Commercial Belge [= RDC] = Tijdschrift voor Belgisch Handelsrecht [=TBH] (1998)Detailed reference viewed: 4 (0 ULg)
IUFRO guidelines for designing multipurpose resource inventories: a project of IUFRO Research group 4.02.02 Vienna
in LUND, H. Gyde (Ed.) IUFRO Guidelines for designing multipurpose resource inventories: a project of IUFRO Research group 4.02.02 Vienna: IUFRO World Series (1998)Detailed reference viewed: 14 (1 ULg)
Cell cycle-related changes in repopulating capacity of human mobilized peripheral blood CD34(+) cells in non-obese diabetic/severe combined immune-deficient mice.
GOTHOT, André ; ; et al
in Blood (1998), 92(8), 2641-9
Most primitive hematopoietic progenitor cells reside in vivo within the G0/G1 phase of the cell cycle. By simultaneous DNA/RNA staining it is possible to distinguish G0 and G1 states and to isolate cells ... [more ▼]
Most primitive hematopoietic progenitor cells reside in vivo within the G0/G1 phase of the cell cycle. By simultaneous DNA/RNA staining it is possible to distinguish G0 and G1 states and to isolate cells in defined phases of the cell cycle. We report here the use of cell cycle fractionation to separate human mobilized peripheral blood (MPB) CD34(+) cells capable of repopulating the bone marrow (BM) of non-obese diabetic/severe combined immune-deficient (NOD/SCID) mice. In freshly isolated MPB, repopulating cells were predominant within the G0 phase, because transplantation of CD34(+) cells residing in G0 (G0CD34(+)) resulted on average in a 16.6- +/- 3.2-fold higher BM chimerism than infusion of equal numbers of CD34(+) cells isolated in G1. We then investigated the effect of ex vivo cell cycle progression, in the absence of cell division, on engraftment capacity. Freshly isolated G0CD34(+) cells were activated by interleukin-3 (IL-3), stem cell factor (SCF), and flt3-ligand (FL) for a 36-hour incubation period during which a fraction of cells progressed from G0 into G1 but did not complete a cell cycle. The repopulating capacity of stimulated cells was markedly diminished compared with that of unmanipulated G0CD34(+) cells. Cells that remained in G0 during the 36-hour incubation period and those that traversed into G1 were sorted and assayed separately in NOD/SCID recipients. The repopulating ability of cells remaining in G0 was insignificantly reduced compared with that of unstimulated G0CD34(+) cells. On the contrary, CD34(+) cells traversing from G0 into G1 were largely depleted of repopulating capacity. Similar results were obtained when G0CD34(+) cells were activated by the combination of thrombopoietin-SCF-FL. These studies provide direct evidence of the quiescent nature of cells capable of repopulating the BM of NOD/SCID mice. Furthermore, these data also demonstrate that G0-G1 progression in vitro is associated with a decrease in engraftment capacity. [less ▲]Detailed reference viewed: 11 (0 ULg)
Compte rendu de N.-Y. TONNERRE, Naissance de la Bretagne
in Moyen Age (Le) (1998)Detailed reference viewed: 4 (0 ULg)
Development of an in vitro culture method of very immature embryos to assist wide interspecific hybridisation in Phaseolus.
; Baudoin, Jean-Pierre ; Mergeai, Guy
in 3rd European conference on grain legumes : Opportunities for high quality, healthy and added-value crops to meet European demands, 14-19 November 1998, Valladolid, Spain (1998)Detailed reference viewed: 10 (5 ULg)
Anemia in children with cancer is associated with decreased erythropoietic activity and not with inadequate erythropoietin production.
; Beguin, Yves ; et al
in Blood (1998), 92(5), 1793-8
A defect in erythropoietin (EPO) production has been advocated as being the main cause of anemia presented at time of diagnosis or during treatment by adults with solid tumors. On the basis of this defect ... [more ▼]
A defect in erythropoietin (EPO) production has been advocated as being the main cause of anemia presented at time of diagnosis or during treatment by adults with solid tumors. On the basis of this defect, anemic cancer patients, both adults and children, have been treated with recombinant human EPO (rHuEPO). To further elucidate the pathophysiology of anemia in children with cancer, we measured serum soluble transferrin receptor (sTfR), a quantitative marker of erythropoiesis, and serum EPO at time of diagnosis and during chemotherapy in children suffering from solid tumor or leukemia. We determined serum EPO in 111 children (55 leukemia, 56 solid tumors) at time of diagnosis. In the last 44 patients (23 leukemia and 21 solid tumors), sTfR levels were also measured. Serum EPO together with sTfR levels were also determined in 60 children receiving chemotherapy (29 leukemia, 31 solid tumors). These results were compared with those obtained from appropriate control groups. In all patients, we found a highly significant correlation between the logarithm of EPO (log[EPO]) and the hemoglobin (Hb) level. In all subsets of patients, sTfR levels were inappropriately low for the degree of anemia. Neither leukemic nor solid tumor groups showed a significant inverse relationship between log(sTfR) and the Hb level as would be expected in anemic patients with appropriate marrow response. Thus, in children with cancer, anemia is associated with a decreased total bone marrow erythropoietic activity which, in contrast to what has been reported in anemic cancer adults, is not related to defective EPO production. [less ▲]Detailed reference viewed: 19 (1 ULg)
Influence of marrow erythropoietic activity on serum erythropoietin levels after autologous hematopoietic stem cell transplantation.
Beguin, Yves ; Baron, Frédéric ; Fillet, Georges
in Haematologica (1998), 83(12), 1076-81
BACKGROUND AND OBJECTIVE: Serum erythropoietin (sEpo) concentration depends primarily on the rate of renal production in response to hypoxia. However, sEpo levels increase inappropriately after ... [more ▼]
BACKGROUND AND OBJECTIVE: Serum erythropoietin (sEpo) concentration depends primarily on the rate of renal production in response to hypoxia. However, sEpo levels increase inappropriately after conditioning for autologous stem cell transplantation (ASCT) before progressively returning to adequate levels. We investigated the possible influence of erythropoietic activity on these observations. DESIGN AND METHODS: Forty patients undergoing an ASCT, 8 with bone marrow (BMT) and 32 with peripheral blood stem cells (PBSC), were separated into 3 groups. Group 1 was formed of the 8 BMT patients (median time to 1% reticulocytes: 39 days), group 2 of 16 PBSC patients with relatively slow erythroid engraftment (> or = 15 days to 1% reticulocytes, median 19 days) and group 3 of 16 PBSC patients with prompt erythroid recovery (< 15 days to 1% reticulocytes, median 13 days). Marrow erythroid activity was assessed by serum transferrin receptor levels (sTfR). Serum Epo (sEpo) levels were expressed in relation to the degree of anemia as observed/predicted (O/P) ratios of (O/P) log (sEpo). RESULTS: Serum sTfR levels decreased by more than 50% in all 3 groups after conditioning, reaching their nadir on day 7. Nadir values doubled by day 28 in group 3, day 60 in group 2, but not within 100 days in group 1. O/P sEpo ratios increased inappropriately in all 3 groups after conditioning but then declined at very differing speeds in the 3 groups. In group 1, ratios remained above 1.10 through to day 28 and above 1.00 through to day 42, before leveling off at around 1.00 thereafter. In group 2, ratios remained above 1.00 through to day 14, than decreased to a minimum of 0.89 by day 42 before returning to 1.00 by day 100. In group 3, ratios decreased to 0.84 by day 21 and remained below 0.90 thereafter. INTERPRETATION AND CONCLUSIONS: We conclude that sEpo levels are not only influenced by tissue oxygenation but also depend on the mass of erythroid precursors in the bone marrow. This may be the main explanation for the observed changes in sEpo levels during ASCT. [less ▲]Detailed reference viewed: 11 (3 ULg)
L'hospitalité, la charité et le soin aux malades à Stavelot-Malmedy au Moyen Age (VIIe-XIIe siècles)
in Revue Bénédictine (1998)Detailed reference viewed: 8 (0 ULg)
Stress coupling between earthquakes in Northwest Turkey and the North Aegean Sea
; Hubert, Aurelia ;
in Journal of Geophysical Research (1998), 103Detailed reference viewed: 10 (4 ULg)
Les aires protégées dans la tourmente. Evolution de la situation de 1990 à 1996
in Cahiers d'Ethologie (1998), 18(2), 161-174
The Akagera National Park (ANP) and the Volcano National Park (VNP) are the main protected areas in Rwanda. Following civil war that bloodstained the country since 1990, the destiny of these two parks ... [more ▼]
The Akagera National Park (ANP) and the Volcano National Park (VNP) are the main protected areas in Rwanda. Following civil war that bloodstained the country since 1990, the destiny of these two parks diverged. The VNP, dedicated to the conservation of one of the last population of mountain gorillas and of their habitat, recovered somehow the same situation as before the war despite some gorillas were lost. The ANP, on the other hand, has been invaded by large herds of domestic cattle, which resulted in serious injuries at its integrity. It seems that two-thirds of the park are on the way to be sacrificed and will be devoted to human and cattle settlement. The fate of the remaining third is still undecided. About 63 years after the park was created, the golden era of this protected area of international reputation seems close to an end... But it is the conservationists' duty to carry on. [less ▲]Detailed reference viewed: 14 (4 ULg)
Quantitative palynology of latest Famennian events in the Sauerland.
in Newsletter. Subcommission on Devonian Stratigraphy (1998), 15Detailed reference viewed: 12 (0 ULg)
Bronchiolitis obliterans organizing pneumonia and ulcerative colitis after allogeneic bone marrow transplantation.
Baron, Frédéric ; ; et al
in Bone Marrow Transplantation (1998), 21(9), 951-4
A 37-year-old man with acute myeloblastic leukemia in first remission developed ulcerative colitis and bronchiolitis obliterans organizing pneumonia (BOOP) 7 months after bone marrow transplantation (BMT ... [more ▼]
A 37-year-old man with acute myeloblastic leukemia in first remission developed ulcerative colitis and bronchiolitis obliterans organizing pneumonia (BOOP) 7 months after bone marrow transplantation (BMT) from an HLA-matched brother who suffered from severe Crohn's disease. BOOP occurred 20 days after idiopathic interstitial pneumonia, in the context of severe ulcerative colitis. Lung and colon biopsies showed no signs of CMV infection or GVHD. The patient was treated with oral methylprednisolone 1 mg/kg/day and his clinical status and chest X-ray improved slowly. Remarkably, the symptoms of colitis also resolved with prednisone therapy and he is now symptom-free. We hypothesize that ulcerative colitis may have been transmitted from donor to recipient (adoptive autoimmunity) and that it was complicated by BOOP. However, other factors such as CMV may have contributed to the occurrence of BOOP. [less ▲]Detailed reference viewed: 67 (2 ULg)
Hépatite à virus G: mythe ou réalité? VHG/GBV-C: diagnostic, épidémiologie, risque transfusionnel et pathogénicité
Gerard, Christiane ; Vaira, Dolorès ; Delwaide, Jean et al
in Revue Médicale de Liège (1998), 53(9), 524-528
The recently discovered G virus (also called either GBV-C or HGV) is transmitted by blood transfusion as well as by sexual intercourse. The global prevalence of GBV-C is high, not only in those groups ... [more ▼]
The recently discovered G virus (also called either GBV-C or HGV) is transmitted by blood transfusion as well as by sexual intercourse. The global prevalence of GBV-C is high, not only in those groups classically known to be exposed to parenteral risks (i.v. drug users, polytransfused patients), but also in the blood donors population. The diagnosis of active infection lies on the search of GBV-C RNA by Polymerase Chain Reaction whereas that of resolved (past) infection lies on the presence of specific antibodies. Till now, it has not been possible to correlate convincingly the presence of GBV-C RNA with any acute or chronic hepatopathy. On the contrary, a lot of arguments tend to suggest that the GBV-C is not pathogenic for the liver, although some modes of transmission are common with those of other (known and probably not known) hepatotropic viruses. According to the actual knowledge of the consequences of GBV-C infection, it appears as non relevant to instaure a systematic screening of this new virus in blood donors. [less ▲]Detailed reference viewed: 26 (2 ULg)
Haematopoietic stem cell transplantation for sickle cell anaemia: the first 50 patients transplanted in Belgium.
; ; et al
in Bone Marrow Transplantation (1998), 22(1), 1-6
Fifty patients affected by sickle cell anaemia underwent transplantation of HLA-identical haematopoietic stem cells (bone marrow, 48; cord blood, 2). Two groups of patients were considered for ... [more ▼]
Fifty patients affected by sickle cell anaemia underwent transplantation of HLA-identical haematopoietic stem cells (bone marrow, 48; cord blood, 2). Two groups of patients were considered for transplantation. Group 1 included 36 permanent residents of a European country who, retrospectively, met the inclusion criteria accepted at a consensus conference held in Seattle in 1990, wherein children were selected because they already had evidence of a morbid course. Group 2 included 14 patients who were transplanted earlier, had not received more than three blood transfusions and were transplanted because they had decided to return to their country of origin. Kaplan-Meier estimates of overall survival, event-free survival and disease-free survival at 11 years of the whole grafted population are 93, 82 and 85%, respectively. In group 1, overall survival, EFS and DFS were 88, 76 and 80% and in group 2, 100, 93 and 93%, respectively. Clinical manifestations of the disease, as well as disease associated haemolytic anaemia, disappeared in all successfully treated patients. Recovery of spleen function was present in seven out of 10 evaluated patients. Adverse events (death, absence of engraftment, mixed chimerism and relapse) occurred more frequently in group 1 than in group 2 (25% vs 7%, P< 0.001). Acute graft-versus-host disease (GVHD) was present in 20 patients (grade I or II, 19; grade III, 1), chronic GVHD in 10 (limited, 7; extensive, 3). One patient developed an acute myeloid leukaemia. Gonadal dysfunction was present in all patients (six boys and eight girls) transplanted close to or after puberty, although transient in one adolescent girl. [less ▲]Detailed reference viewed: 611 (0 ULg)
Protective effect of Ginkgo biloba extract (Egb 761) on functional impairments of mitochondria induced by anoxia-reoxygenation in situ and in vitro
Sluse, Francis ; ; et al
in Packer, L.; Christen, Y. (Eds.) Gingko biloba extract (EGb 761) : lessons from cell biology (1998)Detailed reference viewed: 18 (6 ULg)
Compte rendu de Le culte des saints aux IXe-XIIIe siècles. Actes du colloque de Poitiers
in Moyen Age (Le) (1998)Detailed reference viewed: 2 (0 ULg)