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See detailFormer pour promouvoir un changement des comportements ? Enjeux et méthodes de l'éducation pour la santé
Mairiaux, Philippe ULg; Vandoorne, Chantal ULg

in Formation - Information des salariés (1998, October)

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See detailPrevalence and Molecular Typing of Attaching and Effacing Escherichia Coli among Calf Populations in Belgium
China, B.; Pirson, V.; Mainil, Jacques ULg

in Veterinary Microbiology (1998), 63(2-4), 249-259

Attaching and effacing Escherichia coli are involved in diarrhea in 2 to 8-week old calves. The virulence factors of these bacteria include: (i) the secretion of proteins (i.e. EspB) involved in ... [more ▼]

Attaching and effacing Escherichia coli are involved in diarrhea in 2 to 8-week old calves. The virulence factors of these bacteria include: (i) the secretion of proteins (i.e. EspB) involved in microvilli effacement, (ii) the production of the intimin, a 94 kDa outer membrane protein encoded by the eaeA gene and involved in the intimate attachment of bacteria to epithelial cell and (iii) the production of verotoxins: VT1 and/or VT2. We investigated the presence and the pathotype of these strains in several calf populations by colony hybridization or by genetic amplification. Using the colony hybridization method we showed first that only 5% of calves who died from diarrhea presented EaeA+ E. coli strains and secondly that 19% of healthy calves showed an asymptomatic carriage. However, using colony hybridization and genetic amplification, we identified EaeA+ strains in 91% of calves living in farms with recurrent diarrhea problems. In 66% of the calves, there was a correlation between the presence of AEEC and diarrhea. At the pathotype level, most of the EaeA+ isolates were negative for VT probes. In VT+ bacteria, the majority were VT1+. The number of VT positive bacteria was significantly higher in calves who died from diarrhea than in healthy or sick calves. This underlined the aggravating role of verotoxins in the disease. Moreover, only 25% of the bovine AEEC were positive with the EaeB probe. Surprisingly, the proportion of EaeB+ strains was significantly higher in healthy calves than in other populations. [less ▲]

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See detailAcromégalie : Consultation
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 49

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See detailAcromegalie : Consult
Beckers, Albert ULg; Stevenaert, Achille ULg

in Medical News (1998), 49

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See detailL’évaluation biologique des décharges en relation avec les propriétés de perméabilité
Steyer, E.; Ourth, A.; Hiligsmann, Serge ULg et al

Scientific conference (1998, September 25)

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See detailDiscours de rentrée académique 1998-1999
Legros, Willy ULg

Speech (1998)

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See detailCartilage, un organe à ne pas négliger !
Henrotin, Yves ULg

Article for general public (1998)

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See detailArthrose, une place de choix pour l'aceclofenac.
Henrotin, Yves ULg

Article for general public (1998)

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See detailInfluence of end group and molecular weight on polybutadiene fingerprint secondary ion mass spectra
Vanden Eynde, X.; Bertrand, P.; Dubois, Philippe ULg et al

in Macromolecules (1998), 31(19), 6409-6416

Polybutadiene samples of different molecular weight have been synthesized by anionic polymerization as initiated by sec-butyllithium with low polydispersity and a major content of 1,2-vinyl units. They ... [more ▼]

Polybutadiene samples of different molecular weight have been synthesized by anionic polymerization as initiated by sec-butyllithium with low polydispersity and a major content of 1,2-vinyl units. They have been analyzed by time-of-flight secondary ion mass spectrometry (ToF-SIMS) in order to investigate the sensitivity of this method toward the sec-butyl end group and toward the molecular weight. The SIMS spectra show the characteristic fragment of the end group, C4H9+ at m/z = 57, whose the peak intensity is strongly dependent on the polymer molecular weight, as is the case for almost all the fragment intensities. A model consistent with the peak intensity variations is used to give some new insights into the fragmentation mechanism at the end groups and within the main chain. Moreover, the analysis of the end group fragment allows Mn to be readily determined up to Mn = 4 × 104 from, for example, the Y(53)/Y(57) intensity ratio where Y(53) is the intensity of the deprotonated repeat unit ([M − H]+). Other Mn calibration methods have also been used and are discussed in terms of their accuracy and physical meaning. [less ▲]

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See detailHet Nederlands in Vlaanderen: van taalzorg naar taalbewustzijn
Vromans, Joseph ULg

Conference given outside the academic context (1998)

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See detailCommunication intitulée "La Philosophie Politique des Actions Positives"
Martiniello, Marco ULg

Scientific conference (1998, September 18)

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See detailLa Philosophie Politique des Actions Positives
Martiniello, Marco ULg

Scientific conference (1998, September 18)

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See detailDépopulation de la Ville de Liège et diffusion périurbaine : quels processus pour quelles pistes de solutions ?
Halleux, Jean-Marie ULg

Conference given outside the academic context (1998)

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See detailDecreased Protein Levels of the C-Cbl Protooncogene in Murine Aids
Trebak, Mohamed; Lambert, Charles A; Rahmouni, Souad ULg et al

in Cellular Immunology (1998), 188(2), 151-7

Murine acquired immunodeficiency syndrome (MAIDS) can be viewed as a lymphoproliferative disease which involves B cells as well as T cells from spleen and lymph nodes while thymus and Peyer's patches do ... [more ▼]

Murine acquired immunodeficiency syndrome (MAIDS) can be viewed as a lymphoproliferative disease which involves B cells as well as T cells from spleen and lymph nodes while thymus and Peyer's patches do not participate in the process. The 120-kDa protooncogene product c-Cbl was initially cloned from the murine Cas NS-1 B cell lymphoma. It is a main target of immunoreceptor (TCR and BCR)-mediated protein tyrosine kinase activity. Moreover, recent data suggest that c-Cbl might play a crucial role in the regulation of cell proliferation through regulation of GTP-binding proteins. Therefore, the involvement of c-Cbl was evaluated in the lymphoproliferative disease induced by the MAIDS virus. The expression of the c-Cbl protein was dramatically reduced in the lymph node of infected mice while it remained normal in the thymus. In contrast, the expression of actin, TCR-zeta chain, ZAP-70, and p59(fyn) remained similar in controls and infected mice. Identical results were obtained with sorted B cells and T cells. Surprisingly, a B cell lymphoma line derived from late stage MAIDS mice displayed a normal level of c-Cbl. [less ▲]

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See detailSimultaneous Determination of Methylphenobarbital Enantiomers and Phenobarbital in Human Plasma by on-Line Coupling of an Achiral Precolumn to a Chiral Liquid Chromatographic Column
Ceccato, Attilio ULg; Boulanger, Bruno ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (1998), 819(1-2), 143-53

A fully automated liquid chromatographic (LC) method for the simultaneous determination of methylphenobarbital enantiomers and phenobarbital in human plasma has been developed. The method is based on the ... [more ▼]

A fully automated liquid chromatographic (LC) method for the simultaneous determination of methylphenobarbital enantiomers and phenobarbital in human plasma has been developed. The method is based on the use of a precolumn packed with an internal-surface reversed-phase packing material (LiChrospher ADS) for sample clean-up coupled to LC analysis on a cellulose tris(4-methylbenzoate) based chiral stationary phase (Chiralcel OJ-R). A 100-microliter plasma sample was injected directly on the precolumn packed with LiChrospher RP-18 ADS using a mixture of pH 5.0 phosphate buffer-methanol (97:3, v/v) as washing liquid. The analytes were then eluted in the back-flush mode with the LC mobile phase. The enantiomeric separation of methylphenobarbital was achieved on Chiralcel OJ-R). The retention times were modelled using a D-optimal design with ten experimental points in order to optimise the LC mobile phase for the separation of phenobarbital from the enantiomers of mephobarbital. The factors selected were the acetonitrile content, the pH and the sodium perchlorate concentration in the mobile phase. A Derringer's desirability function was used to find an optimal and robust solution within the experimental domain. The mobile phase selected consisted of a mixture of pH 7.0 phosphate buffer-acetonitrile (60:40, v/v). The elution profiles of phenobarbital, methylphenobarbital and blank plasma samples on the precolumn and the time needed for analyte transfer from the precolumn to the analytical column were then determined. Finally, the method developed was validated. [less ▲]

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