References of "1997"
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See detailTechniques et indications de la bronchoscopie interventionnelle.
CORHAY, Jean-Louis ULg; Bury, Thierry ULg; Radermecker, Marc ULg et al

in Revue Médicale de Liège (1997), 52(10), 657-65

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See detailRoquentin et les types sans importance sociale
Denis, Benoît ULg

in Etudes Françaises (1997), XXXIII(3), 105-119

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See detailBinding sites analysis of the major varicella-zoster virus regulatory IE62 protein
Baudoux, Laurence; Remacle, V.; Defechereux, Patricia et al

Poster (1997)

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See detailTransition probabilities for forbidden lines in Cr II
Quinet, Pascal ULg

in Physica Scripta (1997), 55

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See detailThe Penicillin sensory transducer, blar, involved in the inducibility of beta-lactamase synthesis in bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle
Hardt, Karin; Joris, Bernard ULg; Lepage, Sophie et al

in Molecular Microbiology (1997), 23(5), 935-944

Prediction studies, conformational analyses and membrane-topology mapping lead to the conclusion that the penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in ... [more ▼]

Prediction studies, conformational analyses and membrane-topology mapping lead to the conclusion that the penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis, is embedded in the plasma membrane bilayer via four transmembrane segments TM1-TM4 that forma four-alpha-helix bundle. The extracellular 262-amino-acid-residue polypeptide, S340-R601, that is fused at the carboxy end of TM4, possesses the amino acid sequence signature of a penicilloyl serine transferase. It probably functions as penicillin sensor. As an independent entity, this polypeptide behaves as a high-affinity penicillin-binding protein. As a component of the full-size BlaR, it adopts a different conformation presumably because of interactions with the extracellular 63-amino-acid-residue P53-S115 loop that connects TM2 and TM3. Reception of the penicillin-induced signal requires a precise conformation of the sensor but it does not involve penicilloylation of the serine residue S402 of motif STYK. Signal transmission through the plasma membrane by the four-alpha-helix bundle may proceed in a way comparable to that of the aspartate receptor, Tar. Signal emission in the cytosol by the intracellular 189-amino-acid-residue Y134-K322 loop that connects TM3 and TM4, may proceed via the activation of a putative metallopeptidase. [less ▲]

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See detailVaricella-zoster virus immediate early proteins 4 and 62 interact with cellular transcription factors
Defechereux, Patricia; Baudoux, Laurence; Rentier, Bernard ULg et al

Poster (1997)

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See detailFunctional heterogeneity of human CD34(+) cells isolated in subcompartments of the G0 /G1 phase of the cell cycle.
GOTHOT, André ULg; Pyatt, R.; McMahel, J. et al

in Blood (1997), 90(11), 4384-93

Using simultaneous Hoechst 33342 (Hst) and Pyronin Y (PY) staining for determination of DNA and RNA content, respectively, human CD34(+) cells were isolated in subcompartments of the G0 /G1 phase of the ... [more ▼]

Using simultaneous Hoechst 33342 (Hst) and Pyronin Y (PY) staining for determination of DNA and RNA content, respectively, human CD34(+) cells were isolated in subcompartments of the G0 /G1 phase of the cell cycle by flow cytometric cell sorting. In both bone marrow (BM) and mobilized peripheral blood (MPB) CD34(+) cells, primitive long-term hematopoietic culture-initiating cell (LTHC-IC) activity was higher in CD34(+) cells isolated in G0 (G0CD34(+) cells) than in those residing in G1 (G1CD34(+) cells). However, as MPB CD34(+) cells displayed a more homogeneous cell-cycle status within the G0 /G1 phase and a relative absence of cells in late G1 , DNA/RNA fractionation was less effective in segregating LTHC-IC in MPB than in BM. BM CD34(+) cells belonging to four subcompartments of increasing RNA content within the G0 /G1 phase were evaluated in functional assays. The persistence of CD34 expression in suspension culture was inversely correlated with the initial RNA content of test cells. Multipotential progenitors were present in G0 or early G1 subcompartments, while lineage-restricted granulomonocytic progenitors were more abundant in late G1 . In vitro hematopoiesis was maintained for up to 6 weeks with G0CD34(+) cells, whereas production of clonogenic progenitors was more limited in cultures initiated with G1CD34(+) cells. To test the hypothesis that primitive LTHC-ICs would reenter a state of relative quiescence after in vitro division, BM CD34(+) cells proliferating in ex vivo cultures were identified from their quiescent counterparts by a relative loss of membrane intercalating dye PKH2, and were further fractionated with Hst and PY. The same functional hierarchy was documented within the PKH2(dim) population whereby LTHC-IC frequency was higher for CD34(+) cells reselected in G0 after in vitro division than for CD34(+) cells reisolated in G1 or in S/G2 + M. However, the highest LTHC-IC frequency was found in quiescent PKH2(bright) CD34(+) cells. Together, these results support the concept that cells with distinct hematopoietic capabilities follow different pathways during the G0 /G1 phase of the cell cycle both in vivo and during ex vivo culture. [less ▲]

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See detailNaCl salinity and temperature effects on growth of three wood-rotting basidiomycetes from a Papua New Guinea coastal forest
Castillo Cabello, Gabriel ULg; Demoulin, Vincent ULg

in Mycological Research (1997)

The growth of three wood inhabiting basidiomycetes (Microporus xanthopus, Pycnoporus sanguineus and Schizophyllum commune) isolated from a tropical coastal forest of Papua New Guinea is characterized in ... [more ▼]

The growth of three wood inhabiting basidiomycetes (Microporus xanthopus, Pycnoporus sanguineus and Schizophyllum commune) isolated from a tropical coastal forest of Papua New Guinea is characterized in relation to temperature and NaCl salinity. P. sanguineus and M. xanthopus present a very similar response to NaCl salinity, but P. sanguineus has a wider tolerance for temperature variations. S. commune withstands wide temperature variations, but it is the only one to present a marked salt resistance. This correlates with its abundance along the sea. The presence of P. sanguineus in the same habitat is rather unexpected since it is very sensitive to NaCl salinity. Its high growth rate probably allows it to take advantage of periods of high rains that limit salt accumulation. [less ▲]

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See detailThe relationship between ovarian vascularity and the duration of stimulation in in-vitro fertilization.
Bassil, Salim; Wyns, Christine; Toussaint-Demylle, Dominique et al

in Human Reproduction (1997), 12(6), 1240-5

The role of transvaginal pulsed colour Doppler ultrasound in the assessment of ovarian vascularity was studied in 196 in-vitro fertilization (IVF) cycles. The changes in ovarian blood flow after ... [more ▼]

The role of transvaginal pulsed colour Doppler ultrasound in the assessment of ovarian vascularity was studied in 196 in-vitro fertilization (IVF) cycles. The changes in ovarian blood flow after gonadotrophin-releasing hormone agonist (GnRHa) down-regulation and human menopausal gonadotrophin (HMG) stimulation were determined. The data obtained showed that the ovarian blood flow was significantly improved by oestradiol secretion (P = 0.05) and human chorionic gonadotrophin (HCG) administration (P = 0.003). Folliculogenesis was affected by blood flow supply. The resistance index (RI) value was significantly different (P = 0.05) according to the duration of ovarian stimulation. Patients with a mean RI value >0.56 had a longer stimulation with a significantly lower mean number of oocytes retrieved (P = 0.01) despite the administration of a standard dose of HMG. The RI value is a good indicator of modifications in ovarian vascularization during stimulation. Doppler blood flow measurement could be used to determine the optimal timing for the beginning of HMG administration in patients undergoing ovarian stimulation after down-regulation for IVF treatment. [less ▲]

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See detailCombined Ring Inversion and Side Group Rotation in Geminal Diphosphoryl Substituted Pyrrolinoxyl Radicals: ESR Analysis of Chemical Exchange between Four Nonequivalent Sites
Rockenbauer, Antal; Mercier, Anne; Le Moigne, François et al

in Journal of Physical Chemistry A (1997), 101(43), 7965-7970

The ESR spectra of three diphosphorylated pyrrolidinoxyl radicals have been studied over a large temperature range. While for the trans 2,5-disubstituted compound 1 no line width alternation was found ... [more ▼]

The ESR spectra of three diphosphorylated pyrrolidinoxyl radicals have been studied over a large temperature range. While for the trans 2,5-disubstituted compound 1 no line width alternation was found, for the 2,2-disubstituted compounds 2 and 3, dramatic changes in the spectra were observed as a function of the temperature. These changes were explained by a four-site chemical exchange model including both ring inversion and hindered rotation around the carbon-phosphorus bonds. For radical 3, the presence of additional 5,5-dimethyl substitution can completely block rotations around carbon-phosphorus bonds for certain ring geometries, while for other ring conformations chemical exchange still occurs through combined inversionrotation processes. An effective two-site model composed of nonequivalent sites and a superposition model composed of a pair of exchanging conformers and a pair of nonexchanging conformers were used to simulate spectrum variations. A satisfactory fit was obtained over the entire temperature range investigated. From the temperature dependence of the exchange frequencies the potential barriers for ring inversion (27 kJ/mol), for combined inversion-rotation (11 kJ/mol), and for rotation around the carbon-phosphorus bonds (14 kJ/mol) were estimated. [less ▲]

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See detailThe intensity of emotional facial expression and decoding accuracy
Hess, U; Blairy, Sylvie ULg; Kleck, R

in Journal of Nonverbal Behavior (1997), 21

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See detailSelection of lumped parameter models to characterize vascular properties of pulmonary circulation
LAMBERMONT, Bernard ULg; GERARD, P; DETRY, Olivier ULg et al

in American Journal of Respiratory & Critical Care Medicine (1997), 2

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See detailItinéraires sylvicoles pour la production de frêne de qualité
Claessens, Hugues ULg

Book published by Faculté universitaire des sciences agronomiques de Gembloux (1997)

This paper presents ash in Wallonia (Southern Belgium) and defines its silviculture in the respect of its characteristics (light demanding species, fast juvenile growth, site fertility demanding) and its ... [more ▼]

This paper presents ash in Wallonia (Southern Belgium) and defines its silviculture in the respect of its characteristics (light demanding species, fast juvenile growth, site fertility demanding) and its wood market ( joinery wood, slicing). Two silvicultural ways are presented : “intensive silviculture” and “near-to-nature silviculture”, that are both based upon very similar guidelines (heavy and early thinnings in relation to objective-trees, short rotation, early harvesting) but differ by planting spacings and technical ways used to obtain a high quality butt log. At last, the influence of the proposed ash silviculture is evaluated from a biodiversity point of view, and some ways to preserve the floristic diversity dynamic are suggested. [less ▲]

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See detailA method to correct the shape of pressure waves obtained from pulmonary fluid-filled catheters
LAMBERMONT, Bernard ULg; GERARD, P; DETRY, Olivier ULg et al

in American Journal of Respiratory & Critical Care Medicine (1997), 2

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