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See detailEffects of Perfluorocarbon Emulsions on Cultured Human Endothelial Cells
Mathy-Hartert, M.; Krafft, M. P.; Deby, Christiane ULg et al

in Artificial Cells, Blood Substitutes, & Immobilization Biotechnology (1997), 25(6), 563-75

Perfluorocarbons (PFCs) and their emulsions (PFCEs) were used in organ preservation before transplantation, but not in organ perfusion. Our purpose was to achieve organ perfusion with a PFCE at room ... [more ▼]

Perfluorocarbons (PFCs) and their emulsions (PFCEs) were used in organ preservation before transplantation, but not in organ perfusion. Our purpose was to achieve organ perfusion with a PFCE at room temperature or at 37 degrees C, i. e. with oxygenation, to prevent damages related to reoxygenation after hypoxia. Therefore, we first investigated the effect of such emulsions on endothelial cells, the first cells to be in contact with the emulsion. A stem emulsion was prepared from perfluorooctyl bromide (90% w/v), emulsified with egg yolk phospholipids (2% w/v) and stabilized with a mixed fluorocarbon-hydrocarbon "molecular dowel" (1.4% w/v) (droplets of ca 0.2 micron in diameter). This emulsion was found to be stable when diluted with cell culture media or organ preservation fluids. Endothelial cells from human umbilical vein (HUVECs) were cultured in multiwell plates in M199 medium (with growth factors, 10% foetal calf serum and 5% human serum). Confluent cells were incubated overnight with 51Cr, washed and overlayed with M199 (control) or the above PFCE diluted 2x or 4x with M199 (test). After incubation, the cytotoxicity of the PFCEs was estimated by measuring 51Cr release and observing cell morphology by electron and light microscopy. The percentages of released 51Cr were identical to those of the control cells for the 2x, 3x or 4x diluted PFCEs at 4, 25 or 37 degrees C. After return to the M199 medium, the cells grew and multiplied normally. We conclude that the diluted PFCEs were devoid of cytotoxicity. The 2x diluted PFCE was however partially taken up by the cells: by microscopy, we observed intracellular PFC droplets and by density gradient analysis we found a slight increase in cellular density. The diluted PFCEs were compared to classical organ preservation solutions : HUVECs were incubated with UW (University of Wisconsin) or EC (EuroCollins) solutions at +4 and 37 degrees C (3, 17 or 24 h of incubation). The solutions were observed to be toxic to the cells under these conditions, with cell mortality after return to the M199 medium. This cytotoxicity may be attributed to the high K+ concentration of UW and EC, since similar assays performed on HUVECs with Hank's solution adjusted to 100 mM K+ showed a similar % of 51Cr release. UW and EC are therefore not acceptable as dilution media for PFCEs. [less ▲]

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See detailMultiple Redox Regulation in Nf-Kappab Transcription Factor Activation
Piette, Jacques ULg; Piret, Bernard; Bonizzi, Giuseppina et al

in Biological Chemistry (1997), 378(11), 1237-45

The well-known Rel/NF-kappaB family of vertebrate transcription factors comprises a number of structurally related, interacting proteins that bind DNA as dimers and whose activity is regulated by ... [more ▼]

The well-known Rel/NF-kappaB family of vertebrate transcription factors comprises a number of structurally related, interacting proteins that bind DNA as dimers and whose activity is regulated by subcellular location. This family includes many members (p50, p52, RelA, RelB, c-Rel, ...), most of which can form DNA-binding homo- or hetero-dimers. All Rel proteins contain a highly conserved domain of approximately 300 amino-acids, called the Rel homology domain (RH), which contains sequences necessary for the formation of dimers, nuclear localization, DNA binding and IkappaB binding. Nuclear expression and consequent biological action of the eukaryotic NF-kappaB transcription factor complex are tightly regulated through its cytoplasmic retention by ankyrin-rich inhibitory proteins known as IkappaB. The IkappaB proteins include a group of related proteins that interact with Rel dimers and regulate their activities. The interaction of a given IkappaB protein with a Rel complex can affect the Rel complex in distinct ways. In the best characterized example, IkappaB-alpha interacts with a p50/RelA (NF-kappaB) heterodimer to retain the complex in the cytoplasm and inhibit its DNA-binding activity. The NF-kappaB/IkappaB-alpha complex is located in the cytoplasm of most resting cells, but can be rapidly induced to enter the cell nucleus. Upon receiving a variety of signals, many of which are probably mediated by the generation of reactive oxygen species (ROS), IkappaB-alpha undergoes phosphorylation at serine residues by a ubiquitin-dependent protein kinase, is then ubiquitinated at nearby lysine residues and finally degraded by the proteasome, probably while still complexed with NF-kappaB. Removal of IkappaB-alpha uncovers the nuclear localization signals on subunits of NF-kappaB, allowing the complex to enter the nucleus, bind to DNA and affect gene expression. Like proinflammatory cytokines (e.g. IL-1, TNF), various ROS (peroxides, singlet oxygen, ...) as well as UV (C to A) light are capable of mediating NF-kappaB nuclear translocation, while the sensor molecules which are sensitive to these agents and trigger IkappaB-alpha proteolysis are still unidentified. We also show that a ROS-independent mechanism is activated by IL-1beta in epithelial cells and seems to involve the acidic sphingomyelinase/ceramide transduction pathway. [less ▲]

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See detailHigh Rise Navigation Locks - Feasibility Study of 80 m and 113 m Rise Locks
Rigo, Philippe ULg; Marchal, Jean ULg; Rodriguez, S.

in VII Congreso Nacional de Hidraulica (1997, November)

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See detailEvaluation isocinétique concentrique du genou chez le patient gonarthrosique
Henrotin, Yves ULg; Croisier, Jean-Louis ULg; Dumont, R. et al

in Revue du Rhumatisme et des Maladies Osteo-Articulaires (1997, November), 11

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See detailIntérêt de l'effort excentrique dans la rééducation des tendinopathies
Croisier, Jean-Louis ULg

in Actes du colloque «La pathologie du tendon d'Achille» (1997, November)

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See detailIsokinetic testing of knee musculature in patient with unilateral gonarthrosis
Henrotin, Yves ULg; Croisier, Jean-Louis ULg; Dumont, R. et al

in Revue du Rhumatisme (English ed.) (1997, November), 11

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See detailTriblock copolymer based thermoplastic elastomeric gels of a large service temperature range :preparation and characterization
Yu, Jian Ming; Jérôme, Robert ULg; Overbergh, Noël et al

in Macromolecular Chemistry and Physics (1997), 198(11), 3719-3735

Poly(methyl methacrylate)-block-polybutadiene-block-poly(methyl methacrylate) (MBM) triblock copolymers and their hydrogenated counterparts with poly(ethylene-co-1,2-butylene) midblock (MEBM) were swollen ... [more ▼]

Poly(methyl methacrylate)-block-polybutadiene-block-poly(methyl methacrylate) (MBM) triblock copolymers and their hydrogenated counterparts with poly(ethylene-co-1,2-butylene) midblock (MEBM) were swollen by an aliphatic oil of high boiling point which is a selective solvent for the central block. Thermoreversible gels are accordingly formed by both MBM and MEBM copolymers above a critical polymer content (Cr), which depends on the nature of the midblock and not on the copolymer molecular weight, at least in the investigated range. Cr has been found to be 5 wt.-% for an MBM block copolymer and 2 wt.-% for MEBM copolymers of various molecular weights. Gels of MEBM triblock copolymers exhibit interesting mechanical properties, such as high elon-gation at break (up to 870%) and high tensile strength (32 kPa). The most interesting fea-ture of the MEBM gels is an upper service temperature as high as 170°C, thus more than 100°C higher than the value (47°C) reported for gels of an SEBS copolymer (S = polystyrene) of comparable molecular weight (100000) and composition (ca. 30 wt.-% hard block). The morphology of MEBM gels was studied by scanning electron microscopy (SEM) and found to be cocontinuous in case of a gel containing 20 wt.-% copolymer. [less ▲]

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See detailLes mutations NEM-1 dans les familles belges
Beckers, Albert ULg

Scientific conference (1997, November)

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See detailHST confirmation of the lensed quasar J03.13.
Surdej, Jean ULg; Claeskens, Jean-François ULg; Remy, M. et al

in Astronomy and Astrophysics (1997), 327

In order to definitely prove the claim by Claeskens et al. (1996A&A...305L...9C) that J03.13 is a doubly imaged quasar, WFPC2 direct imaging and FOS spectroscopy of this compact system have been obtained ... [more ▼]

In order to definitely prove the claim by Claeskens et al. (1996A&A...305L...9C) that J03.13 is a doubly imaged quasar, WFPC2 direct imaging and FOS spectroscopy of this compact system have been obtained with HST. These textbook case observations clearly show that J03.13 consists of two point-like images separated by 0.849"+/-0.001" with a magnitude difference of 2.14+/-0.03 in V and I. We see no trace of a lensing galaxy. From the FOS observations, we find that J03.13 A and B have identical spectra within the measurement uncertainties. The data also show that spectroscopic contamination of B by A is negligible. We do confirm the redshift z=2.545 for J03.13 A and B (emission-lines due to Ly-beta lambda1025 and O VI lambdalambda1031, 1037 are also detected), first derived from an unresolved ground-based spectrum of the two components by Claeskens et al. We also find that the absorption line system at z=2.344 (CIV and Ly-alpha) is present in the spectra of A and B. On the contrary, the absorption line system at z=1.085 (MgII) is only present in the spectrum of J03.13 A. If this latter system is associated with the lens, we may expect that image A consists of two radially merging images with a very small angular separation. From the WFPC2 imagery, we can rule out the presence of a third component fainter than A by up to 5.2mag. with an angular separation >=0.13". [less ▲]

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See detailBoth Pituitary and Placental Growth Hormone Transcripts Are Expressed in Human Peripheral Blood Mononuclear Cells (Pbmc)
Melen-Lamalle, Laurence ULg; Hennen, Georges ULg; Dullaart, R. P. et al

in Clinical & Experimental Immunology (1997), 110(2), 336-40

The hGH-V gene codes for a variant of human pituitary growth hormone (hGH-N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH-N. Until now the hGH-V gene was considered ... [more ▼]

The hGH-V gene codes for a variant of human pituitary growth hormone (hGH-N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH-N. Until now the hGH-V gene was considered to be exclusively expressed in human placenta, where it replaces maternal circulating hGH-N at the end of pregnancy. In this study we investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis hGH-N, and hGH-V, gene expression in PBMC in men, women and pregnant women. We have demonstrated that hGH-N and hGH-V transcripts are simultaneously produced by PBMC in both men and women as well as pregnant women. The PBMC of a PIT-1-negative woman expressed only the hGH-V transcript, but not the hGH-N one as expected. In conclusion, hGH-V mRNA is expressed by cells other than the syncytiotrophoblast, is not regulated by PIT-1, and may be involved in immune regulation, as is pituitary GH. [less ▲]

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See detailThe use of a food frequency questionnaire in an adolescent population.
Paulus, D.; Saint-Remy, Annie ULg; Jeanjean, Marcelle ULg et al

Conference (1997, November)

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See detailGare aux cercles vicieux…
Durand, Pascal ULg

Article for general public (1997)

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See detailAmendements de bassins versants dans les Ardennes belges : effets sur l’eau de percolation et l’eau à l’exutoire
Carnol, Monique ULg

Conference given outside the academic context (1997)

Alors que des essais d’amendements en forêt se sont révélés peu rentables, un nouvel intérêt s’est manifesté pour ce type de traitement dans les années 1980, après les observations de jaunissement et de ... [more ▼]

Alors que des essais d’amendements en forêt se sont révélés peu rentables, un nouvel intérêt s’est manifesté pour ce type de traitement dans les années 1980, après les observations de jaunissement et de chutes d’aiguilles inhabituelles, notamment chez l’épicéa. Les causes de ce ‘nouveau dépérissement forestier’, parmi lesquelles citons, l’acidification des sols, la saturation de l’écosystème en azote et les déséquilibres nutritionnels, sont supposées agir de manière synergique. Les forêts ardennaises n’ont pas été épargnées par ce phénomène, et des symptômes de dépérissement forestier ont été rapportés en 1983 par Weissen (Weissen et al., 1983). Ce dépérissement serait dû à une augmentation de la pollution atmosphérique exacerbant la déficience en magnésium des arbres, qui se développent sur les sols ardennais naturellement pauvres en magnésium (Weissen et al., 1990). L’amendement a donc été conseillé comme mesure de protection et de correction de l’acidification des sols et des déséquilibres nutritionnels chez les arbres. Cependant, des effets secondaires potentiels, en particulier sur l’eau à l’exutoire (nappes et eau potable) et sur la solution du sol (nutrition des arbres) doivent être évalués. Dans cette présentation, je résumerai les résultats d’une étude poursuivie dans 4 bassins versants boisés, en majorité, par l’épicéa commun (Picea abies [L.] Karst.). Les deux couples de bassins versants d’une taille d’environ 80 ha sont situées à la Croix Scaille (commune de Gedinne) et dans l’Hertogenwald (Hautes Fagnes), sur sol brun acide. Un bassin versant de chaque couple a été amendé par 3 T/ha de dolomie ((Ca, Mg)CO3 55/40) et de 200 kg/ha de K2SO4 en 1992 et 1993, respectivement. La pluie à découvert, les pluviolessivats (pluie interceptée par les arbres) et la solution du sol ont été analysés mensuellement, l’eau à l’exutoire toutes les 2 semaines. L’analyse en séries temporelles a été utilisée pour détecter des changements significatifs qui surviendraient dans les concentrations en éléments minéraux dans l’eau à l’exutoire et qui seraient dus à l’amendement. Par ailleurs, les flux entrants et sortants du bassin versant ont été calculés de manière à établir le bilan entrée-sortie des éléments dans la forêt et ainsi pouvoir évaluer les pertes de l’amendement vers le ruisseau. Dans le bassin des Hautes Fagnes, la dissolution de la dolomie a influencé de manière significative les concentrations à l’exutoire. Les concentrations en magnésium ont augmenté immédiatement après l’amendement (Fig. 1), probablement à cause de l’écoulement de l’eau de pluie dans les couches supérieures du sol. Quatre années après le traitement, elles restent toujours supérieures aux concentrations observées avant l’amendement. Néanmoins, les quantités de magnésium perdues à l’exutoire sont faibles par rapport aux doses apportées par l’amendement. Dans les 2 bassins amendés, les concentrations du calcium et des autres cations et anions majeurs de la solution du sol n’ont pas été influencées significativement par le traitement. Les concentrations en magnésium dans la solution du sol ont augmenté immédiatement et un an après l’amendement respectivement à environ 5 et 15 cm de profondeur. Les concentrations en calcium dans la solution du sol ont augmenté 3 ans après l’amendement. Ces résultats indiquent que, pour les sols étudiées et la dose d’amendement apportée, une grande partie de l’amendement est retenue dans les sols, et que, jusqu’à présent, les conséquences pour l’eau à l’exutoire sont peu importantes. En effet, seule la concentration en magnésium a augmenté de manière significative alors qu’aucun changement significatif n’a été détecté, jusqu'à présent, dans les teneurs en nitrates (Fig. 2) et en métaux lourds (Cd, Pb, Zn). On peut donc conclure que, sur base de nos analyses et dans la situation actuelle, l’eau de l’exutoire qui alimente le barrage-réservoir de la Gileppe n’a pas subi de modifications importantes à la suite de l’amendement. Il faut cependant souligner que la dissolution de l’amendement peut être lente dans les sols acides, et que des effets ‘à retardement’ sont possibles. De plus, une étude récente, comparant, « in situ », l’effet d’un amendement sur la solution du sol d’une chênaie et d’une pessière de même âge a montré une augmentation de la concentration en nitrates dans la solution de sol de la chênaie. Il faut donc rester prudent lors de l’extrapolation de ces résultats vers d’autres sites. Figure 1. Influence d’un amendement dolomitique (3 T/ha) sur les concentrations en magnésium à l’exutoire d’un bassin versant. Figure 2. Influence d’un amendement dolomitique (3 T/ha) sur les concentrations en nitrate à l’exutoire d’un bassin versant. Références Weissen, F., Letocart, H. & Van Praag, H.J., Bull. Soc. Roy. Bot. Belg. 2, 1-13 (1983) Weissen, F., Hambuckers, A., Van Praag, H.J. and Remacle, J., Plant Soil 128, 59-66 (1990). [less ▲]

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See detailQu'est-ce que le français langue seconde?
Defays, Jean-Marc ULg; Denyer, Monique

Scientific conference (1997, November)

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See detailEssais variétés : la chasse aux effets de competition !
Claustriaux, Jean-Jacques ULg; David, O.; Monod, H. et al

in Perspectives Agricoles (1997), 229

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See detailAlphonse Allais ou la poésie du rire
Defays, Jean-Marc ULg; Dufays, Jean-Louis

Conference given outside the academic context (1997)

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See detailThéories du texte et apprentissage des langues : les grands axes d'une approche interdisciplinaire
Defays, Jean-Marc ULg

in Travaux de linguistique : revue internationale de linguistique Française (1997), 34

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See detailClear Cell Sarcoma: An Extremely Rare Cause of Pleural Disease
BURY, Thierry ULg; HERMANS, Gilberte ULg; Alexis-Agnant, R. et al

in European Respiratory Journal (1997), 10(11), 2653-6

We present the case of a 36 yr old woman with a persisting complaints of left chest pain. A chest radiograph revealed multiple left pleural thickenings. Classical exploration was negative. Thoracic ... [more ▼]

We present the case of a 36 yr old woman with a persisting complaints of left chest pain. A chest radiograph revealed multiple left pleural thickenings. Classical exploration was negative. Thoracic surgery allowed the subtotal removal of a huge pleural tumour. The histological examination revealed a clear cell sarcoma. The literature on this extremely rare tumour is reviewed. [less ▲]

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