References of "1997"
     in
Bookmark and Share    
Peer Reviewed
See detailBoth Pituitary and Placental Growth Hormone Transcripts Are Expressed in Human Peripheral Blood Mononuclear Cells (Pbmc)
Melen-Lamalle, Laurence ULg; Hennen, Georges ULg; Dullaart, R. P. et al

in Clinical & Experimental Immunology (1997), 110(2), 336-40

The hGH-V gene codes for a variant of human pituitary growth hormone (hGH-N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH-N. Until now the hGH-V gene was considered ... [more ▼]

The hGH-V gene codes for a variant of human pituitary growth hormone (hGH-N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH-N. Until now the hGH-V gene was considered to be exclusively expressed in human placenta, where it replaces maternal circulating hGH-N at the end of pregnancy. In this study we investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis hGH-N, and hGH-V, gene expression in PBMC in men, women and pregnant women. We have demonstrated that hGH-N and hGH-V transcripts are simultaneously produced by PBMC in both men and women as well as pregnant women. The PBMC of a PIT-1-negative woman expressed only the hGH-V transcript, but not the hGH-N one as expected. In conclusion, hGH-V mRNA is expressed by cells other than the syncytiotrophoblast, is not regulated by PIT-1, and may be involved in immune regulation, as is pituitary GH. [less ▲]

Detailed reference viewed: 35 (4 ULg)
Peer Reviewed
See detailThe use of a food frequency questionnaire in an adolescent population.
Paulus, D.; Saint-Remy, Annie ULg; Jeanjean, Marcelle ULg et al

Conference (1997, November)

Detailed reference viewed: 10 (3 ULg)
Full Text
See detailGare aux cercles vicieux…
Durand, Pascal ULg

Article for general public (1997)

Detailed reference viewed: 4 (1 ULg)
See detailAmendements de bassins versants dans les Ardennes belges : effets sur l’eau de percolation et l’eau à l’exutoire
Carnol, Monique ULg

Conference given outside the academic context (1997)

Alors que des essais d’amendements en forêt se sont révélés peu rentables, un nouvel intérêt s’est manifesté pour ce type de traitement dans les années 1980, après les observations de jaunissement et de ... [more ▼]

Alors que des essais d’amendements en forêt se sont révélés peu rentables, un nouvel intérêt s’est manifesté pour ce type de traitement dans les années 1980, après les observations de jaunissement et de chutes d’aiguilles inhabituelles, notamment chez l’épicéa. Les causes de ce ‘nouveau dépérissement forestier’, parmi lesquelles citons, l’acidification des sols, la saturation de l’écosystème en azote et les déséquilibres nutritionnels, sont supposées agir de manière synergique. Les forêts ardennaises n’ont pas été épargnées par ce phénomène, et des symptômes de dépérissement forestier ont été rapportés en 1983 par Weissen (Weissen et al., 1983). Ce dépérissement serait dû à une augmentation de la pollution atmosphérique exacerbant la déficience en magnésium des arbres, qui se développent sur les sols ardennais naturellement pauvres en magnésium (Weissen et al., 1990). L’amendement a donc été conseillé comme mesure de protection et de correction de l’acidification des sols et des déséquilibres nutritionnels chez les arbres. Cependant, des effets secondaires potentiels, en particulier sur l’eau à l’exutoire (nappes et eau potable) et sur la solution du sol (nutrition des arbres) doivent être évalués. Dans cette présentation, je résumerai les résultats d’une étude poursuivie dans 4 bassins versants boisés, en majorité, par l’épicéa commun (Picea abies [L.] Karst.). Les deux couples de bassins versants d’une taille d’environ 80 ha sont situées à la Croix Scaille (commune de Gedinne) et dans l’Hertogenwald (Hautes Fagnes), sur sol brun acide. Un bassin versant de chaque couple a été amendé par 3 T/ha de dolomie ((Ca, Mg)CO3 55/40) et de 200 kg/ha de K2SO4 en 1992 et 1993, respectivement. La pluie à découvert, les pluviolessivats (pluie interceptée par les arbres) et la solution du sol ont été analysés mensuellement, l’eau à l’exutoire toutes les 2 semaines. L’analyse en séries temporelles a été utilisée pour détecter des changements significatifs qui surviendraient dans les concentrations en éléments minéraux dans l’eau à l’exutoire et qui seraient dus à l’amendement. Par ailleurs, les flux entrants et sortants du bassin versant ont été calculés de manière à établir le bilan entrée-sortie des éléments dans la forêt et ainsi pouvoir évaluer les pertes de l’amendement vers le ruisseau. Dans le bassin des Hautes Fagnes, la dissolution de la dolomie a influencé de manière significative les concentrations à l’exutoire. Les concentrations en magnésium ont augmenté immédiatement après l’amendement (Fig. 1), probablement à cause de l’écoulement de l’eau de pluie dans les couches supérieures du sol. Quatre années après le traitement, elles restent toujours supérieures aux concentrations observées avant l’amendement. Néanmoins, les quantités de magnésium perdues à l’exutoire sont faibles par rapport aux doses apportées par l’amendement. Dans les 2 bassins amendés, les concentrations du calcium et des autres cations et anions majeurs de la solution du sol n’ont pas été influencées significativement par le traitement. Les concentrations en magnésium dans la solution du sol ont augmenté immédiatement et un an après l’amendement respectivement à environ 5 et 15 cm de profondeur. Les concentrations en calcium dans la solution du sol ont augmenté 3 ans après l’amendement. Ces résultats indiquent que, pour les sols étudiées et la dose d’amendement apportée, une grande partie de l’amendement est retenue dans les sols, et que, jusqu’à présent, les conséquences pour l’eau à l’exutoire sont peu importantes. En effet, seule la concentration en magnésium a augmenté de manière significative alors qu’aucun changement significatif n’a été détecté, jusqu'à présent, dans les teneurs en nitrates (Fig. 2) et en métaux lourds (Cd, Pb, Zn). On peut donc conclure que, sur base de nos analyses et dans la situation actuelle, l’eau de l’exutoire qui alimente le barrage-réservoir de la Gileppe n’a pas subi de modifications importantes à la suite de l’amendement. Il faut cependant souligner que la dissolution de l’amendement peut être lente dans les sols acides, et que des effets ‘à retardement’ sont possibles. De plus, une étude récente, comparant, « in situ », l’effet d’un amendement sur la solution du sol d’une chênaie et d’une pessière de même âge a montré une augmentation de la concentration en nitrates dans la solution de sol de la chênaie. Il faut donc rester prudent lors de l’extrapolation de ces résultats vers d’autres sites. Figure 1. Influence d’un amendement dolomitique (3 T/ha) sur les concentrations en magnésium à l’exutoire d’un bassin versant. Figure 2. Influence d’un amendement dolomitique (3 T/ha) sur les concentrations en nitrate à l’exutoire d’un bassin versant. Références Weissen, F., Letocart, H. & Van Praag, H.J., Bull. Soc. Roy. Bot. Belg. 2, 1-13 (1983) Weissen, F., Hambuckers, A., Van Praag, H.J. and Remacle, J., Plant Soil 128, 59-66 (1990). [less ▲]

Detailed reference viewed: 48 (1 ULg)
Full Text
Peer Reviewed
See detailWhole-Body 18fdg Positron Emission Tomography in the Staging of Non-Small Cell Lung Cancer
Bury, Thierry ULg; Dowlati, A.; Paulus, Patrick et al

in European Respiratory Journal (1997), 10(11), 2529-34

Despite advances in morphological imaging, some patients with lung cancer are found to have nonresectable disease at surgery or die of recurrence within yr of surgery. We performed a prospective study in ... [more ▼]

Despite advances in morphological imaging, some patients with lung cancer are found to have nonresectable disease at surgery or die of recurrence within yr of surgery. We performed a prospective study in 109 patients to compare the accuracy of whole-body positron emission tomography (PET) using fluorine-18 deoxyglucose (18FDG) and conventional imaging (CI) methods for the staging of non-small cell lung cancer (NSCLC). When CI or PET study suggested metastatic disease, confirmation was obtained by biopsy or follow-up information. As compared to CI, 18FDG-PET correctly changed the N stage in 22 patients (33%) and the M stage in 15 patients (14%). For the detection of distant metastases, PET study showed five false-positive sites and no false-negative cases. Currently, the accuracy of PET in the detection of M stage is 96%. Our study shows that visual interpretation of whole-body fluorine-18 deoxyglucose-positron emission tomography images can improve the diagnostic accuracy in the staging of non-small cell lung cancer. Further experience is needed to establish if metabolic imaging would be a cost-effective tool in the future management of lung cancer. [less ▲]

Detailed reference viewed: 21 (2 ULg)
See detailQu'est-ce que le français langue seconde?
Defays, Jean-Marc ULg; Denyer, Monique

Scientific conference (1997, November)

Detailed reference viewed: 33 (9 ULg)
Full Text
Peer Reviewed
See detailEssais variétés : la chasse aux effets de competition !
Claustriaux, Jean-Jacques ULg; David, O.; Monod, H. et al

in Perspectives Agricoles (1997), 229

Detailed reference viewed: 8 (1 ULg)
See detailOceanological applications of stable isotope ratios
Dauby, Patrick ULg

Scientific conference (1997, November)

Detailed reference viewed: 4 (0 ULg)
See detailAlphonse Allais ou la poésie du rire
Defays, Jean-Marc ULg; Dufays, Jean-Louis

Conference given outside the academic context (1997)

Detailed reference viewed: 41 (1 ULg)
Full Text
Peer Reviewed
See detailThéories du texte et apprentissage des langues : les grands axes d'une approche interdisciplinaire
Defays, Jean-Marc ULg

in Travaux de linguistique : revue internationale de linguistique Française (1997), 34

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailClear Cell Sarcoma: An Extremely Rare Cause of Pleural Disease
BURY, Thierry ULg; HERMANS, Gilberte ULg; Alexis-Agnant, R. et al

in European Respiratory Journal (1997), 10(11), 2653-6

We present the case of a 36 yr old woman with a persisting complaints of left chest pain. A chest radiograph revealed multiple left pleural thickenings. Classical exploration was negative. Thoracic ... [more ▼]

We present the case of a 36 yr old woman with a persisting complaints of left chest pain. A chest radiograph revealed multiple left pleural thickenings. Classical exploration was negative. Thoracic surgery allowed the subtotal removal of a huge pleural tumour. The histological examination revealed a clear cell sarcoma. The literature on this extremely rare tumour is reviewed. [less ▲]

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailAnalysis of the Mutations Induced in the E. Coli Lac Z Gene by a Psoralen Analog
Matroule, J. Y.; Collet, M.; Boiteux, S. et al

in Journal of Photochemistry and Photobiology B : Biology (1997), 41(1-2), 36-44

A psoralen in which intracyclic oxygen atoms were replaced by sulfur (7H-thieno [3,2-g]-[1]-[benzothiopyran-7-one) [PSO(S-S)]) was recently synthesized and its photobiological properties were investigated ... [more ▼]

A psoralen in which intracyclic oxygen atoms were replaced by sulfur (7H-thieno [3,2-g]-[1]-[benzothiopyran-7-one) [PSO(S-S)]) was recently synthesized and its photobiological properties were investigated. M13mp19 DNA photosensitization mediated by PSO (S-S) followed by transfection into competent E. coli gave rise to a very low phage progeny showing the high aptitude of this compound to modify DNA. In order to characterize the role of oxidative damages in the photosensitized reaction mediated by PSO(S-S), plasmid bearing the gene encoding the formamidopyrimidine DNA glycosylase (Fpg) under the control of the inducible lac Z promoter was transfected in E. coli. Overexpression of Fpg was induced by addition of isopropyl-beta-D-thio-galactopyranoside (IPTG) to the cells and monitored by western blot analysis. Fpg overexpression did not influence the rate of M13mp19 DNA photoinactivation by PSO(S-S) neither the mutation frequency measured by the expression of beta-galactosidase encoded by the lac Z gene beared by M13mp19. Analysis of the mutation patterns recorded with or without Fpg overexpression showed that several G to T transversions due to oxidative damages were repaired by Fpg. These data show that oxidative DNA damages generated during PSO(S-S) photosensitization have only limited biological implications measured in terms of DNA photoinactivation. [less ▲]

Detailed reference viewed: 8 (3 ULg)
Full Text
Peer Reviewed
See detailIPM in Asia - A review of existing projects in the Philippines and Indonesia
Wynn, Nigel R.; Schiffers, Bruno ULg; Seghers, N. et al

in Agro-Chemicals News in Brief (1997), XX(2), 1-18

Integrated Pest Management means a pest management system that, in the context of the associated environment and the population dynamics of the pest species, utilizes ail suitable techniques and methods ... [more ▼]

Integrated Pest Management means a pest management system that, in the context of the associated environment and the population dynamics of the pest species, utilizes ail suitable techniques and methods in as compatible a manner as possible and maintains the pest populations at levels below those causing economically unacceptable damage or 1055. FAO-Code- of Conduct 1984. [less ▲]

Detailed reference viewed: 20 (5 ULg)
See detailLe transport intermodal en Belgique
Jacob, Damien ULg

in Le rail et la route : le commerce ou la dispute (1997)

Detailed reference viewed: 32 (0 ULg)
Full Text
Peer Reviewed
See detailThe use of a food frequency questionnaire in a adolescent population
Paulus, D.; Saint-Remy, Annie ULg; Jeanjean, Michel et al

in Gaceta Sanitaria (1997, November), 11(suppl 2), 37

Detailed reference viewed: 16 (5 ULg)
See detailLipopeptides alter Bacillus subtilis hydrophobicity by adsorbing onto cell surfaces
Ahimou, François; Razafindralambo, Hary ULg; Paquot, Michel ULg

Poster (1997, October 29)

Detailed reference viewed: 5 (2 ULg)
See detailNitrogen deposition and nitrification in coniferous forests’
Carnol, Monique ULg

Doctoral thesis (1997)

The increased inorganic nitrogen (N) deposition in the last decades has become a major concern for the health of forest ecosystems. High anthropogenic N emissions, mainly from fossil fuel combustion and ... [more ▼]

The increased inorganic nitrogen (N) deposition in the last decades has become a major concern for the health of forest ecosystems. High anthropogenic N emissions, mainly from fossil fuel combustion and livestock agriculture, have resulted in both high gaseous concentrations and high deposition in rainfall and throughfall. In forest ecosystem, where N is no longer limiting to primary production due to high inputs, the excess N is thought to be related to forest decline and a concept of ‘N saturation ‘ has been developed. In particular, N in the form of NH4, in excess to plant and microbial demands could lead to soil acidification if nitrified in the soil and leached, causing loss of base cations or mobilisation of phytotoxic aluminium. Nutrient imbalances due to high soil solution NH4/cation ratios or damaged root systems may also occur. The fate of the incoming NH4 is central to determining the effects on the ecosystem, and is closely related to the controls of nitrification. Although this process has been intensely studied in pure cultures for some nitrifying bacteria, the organisms responsible and controlling factors in acid forest soils are still poorly understood. A better comprehension of the fate of NH4 deposition is necessary to determine ‘Critical Loads for N’, the threshold deposition not damaging to the ecosystem, which is used as a political tool for quantifying pollution limits. In this thesis, I focused on a) the effects of increased (NH4)2SO4 deposition on soil solution chemistry of six coniferous forest sites the presence of live roots, b) the impacts of (NH4)2SO4 deposition on Norway spruce (Picea abies [L.] Karst) and Scots pine (Pinus sylvestris L.) fine roots, and c) the controls of nitrification in an acid forest soil. The work was part of the CEC project ‘CORE’, investigating the effects of atmospheric pollution on nutrient turnover in soils. An identical field experiment was performed in six coniferous sites, situated in five European countries. Chronically increased NH4 deposition by 75 kg N ha-1 a-1 through (NH4)2SO4 application, demonstrated the contrasting responses of the different ecosystems. Soil solution concentrations and yearly ionic fluxes were analysed. (NH4)2SO4 treatment resulted in deposition of 79 to 93 kg N ha-1 a-1 at the different sites. In the two less acidic, clay/clay loam soils, only 6% of the added NH4 was lost through leaching. The two sandy soils lost up to 75% of the added NH4, and the two remaining sites lost ca. 25%. Leaching of added NH4 was thought to be related to soil physico-chemical characteristics, such as pH, C and N content and texture. NO3 leaching was increased at three sites, only 4-9 months after starting the (NH4)2SO4 treatment, with a maximum doubling of concentrations. One sandy soil failed to nitrify under any condition, and the other sandy soil showed high NO3 leaching under all treatments, but no increase due to increased N inputs. The presence of live roots reduced NO3 leaching in two sites, delaying the increase in soil solution NO3 concentrations in response to the (NH4)2SO4 deposition in one of them. In all nitrifying soils, soil solution NO3 concentrations were related to cation concentrations, with Al being the dominant cation in the more acid soils with low base saturation. This experiment demonstrated the importance of soil N storage capacity and nitrification potential in determining the consequences of increased NH4 deposition, and the strong relationship between NO3 and cation leaching. Ionic fluxes and soil solution chemistry were further analysed in one of the six sites (Grizedale, UK). In this Norway spruce stand on clay soil, NO3 fluxes were increased by increased (NH4)2SO4 deposition, and mainly balanced by increased Al losses. This soil had a pHH2O around 3.6, and was characterised by over 90% of the exchange complex being occupied by Al. Independent of treatment, soil solution changed from Ca to Al leaching during the 18 month field experiment, with a decrease in soil solution pH from 4.9 to 3.8. At the end of the experiment, soil solution Al concentrations were higher for the (NH4)2SO4 treatments. It was suggested that nitrification had caused the pH decrease, with a further lowering of the base saturation, linked to a abrupt increase in soil solution Al concentrations. The impacts of increased (NH4)2SO4 deposition and soil characteristics on Norway spruce root biomass and vitality, and on Norway spruce and Scots pine fine root chemistry, were investigated with an ingrowth core technique. The same experiment was performed in a Norway spruce stand on clay soil (Grizedale, UK) and a Scots pine stand on sandy soil (Wekerom, NL), using soil from each of the two sites. For Norway spruce, root biomass and numbers of fine root tips were higher in the organic than in the mineral horizon of the clay and sandy soils. This was related to higher fine root Al and lower Ca contents in the mineral horizon. Root biomass and the proportion of dead roots were higher in the clay soil, compared to the sandy soil, with higher root Al contents, despite lower soil solution Al concentrations than in the sandy soil. For Norway spruce, a negative correlation between root biomass and fine root Al content was established. Enhanced N deposition caused an increase in the total number of root tips and in the proportion of dead roots in the sandy soil. Effects of increased (NH4)2SO4 deposition on root biomass were not significant for the clay soil, yet caused increased fine root N content in the organic horizon for both species. Scots pine fine roots also showed higher Al and lower Ca contents in the mineral horizon. (NH4)2SO4 treatment caused increased fine root Al content and a decreased Mg/Al ratio in the mineral layer of the sandy soil, with opposite effects in the clay soil. This (NH4)2SO4 treatment effect in the sandy soil for Scots pine was the only indication of a potential adverse effect of (NH4)2SO4 deposition on fine roots. Results demonstrated the dominant importance of inherent soil characteristics and the stratification into soil horizons on fine root growth and chemical composition. The effects of temperature, throughfall volume and NH4 deposition on soil solution NO3 concentrations, N2O emissions and numbers of NH4 oxidisers were investigated for the Grizedale soil in a controlled laboratory experiment. Multiple regression and surface response analysis revealed temperature as the most important factor, with an optimum for NO3 leaching and numbers of NH4 oxidisers in the mineral horizon at 11°C. Volume acted independently of temperature with a minimum at 870 mm throughfall 2 weeks-1. The relatively low optimum temperature compared to other studies was explained by the minimum disturbance of the soil in the current study. NO3 fluxes increased quadratically with throughfall volume. N2O fluxes increased quadratically with temperature and throughfall volume, and showed high variability. It was suggested that the temperature optimum for net nitrification depended on the physico-chemical characteristics of the soil and on the activity of decomposers, by competition for O2 and NH4. Optimum temperatures may have been overestimated in previous studies using disturbed soils. The regression model for NO3 leaching derived from the laboratory experiment was applied to data from the previous field experiment and tested with different time intervals for temperature input parameters. A model including two-monthly mean temperatures yielded the best fit between measured and simulated values, as determined by correlation and minimum sum of squared residuals. Simulated NO3 leaching was over-estimated in the second part of the field study. The good correspondence between field temperature frequency distribution and the optimum temperature determined by the regression model, as well as the high correlation between measured and simulated values, demonstrated the adequacy of a quadratic model with a relatively low temperature optimum to describe field NO3 leaching, determined for the same soil with an identical sampling design. [less ▲]

Detailed reference viewed: 36 (5 ULg)