Further Characterization of Cytotoxic T Cells Generated by Short-Term Culture of Human Peripheral Blood Lymphocytes with Interleukin-2 and Anti-Cd3 Mab

in Cancer Immunology, Immunotherapy (1996), 42(6), 369-75

In this study we have specifically investigated the participation of T cells in the cytotoxic activity of peripheral blood lymphocytes (PBL) activated by interleukin-2 (IL-2, 50 U/ml) alone or in ... [more ▼]

In this study we have specifically investigated the participation of T cells in the cytotoxic activity of peripheral blood lymphocytes (PBL) activated by interleukin-2 (IL-2, 50 U/ml) alone or in combination with an anti-CD3 mAb (BMA030, 10 ng/ml, IgG2a). Purified CD3+ T cells, incubated in the presence of the anti-CD3 mAb for 4 days, mediated a cytotoxic activity against HL60 and U937 tumor cell lines. Several findings suggested the involvement of a redirected-cytotoxicity phenomenon, since the lytic process was restricted to target cell lines bearing the high-affinity Fc gamma receptor (Fc gamma RI) and T lymphocytes stimulated by IL-2 alone did not lyse these cell lines. Furthermore, anti-CD3 mAb F(ab')2, anti-CD3 IgG1 (UCHT1), phytohemagglutinin or staphylococcal enterotoxin A did not induce a similar cytotoxic activity in T lymphocytes. The cytotoxic process occurred in the presence of a very low level of anti-CD3 antibodies (in the nanomolar range). The cytotoxic activity of T cells stimulated by IL-2 or by IL-2 + BMA030, against OVCAR-3 cells (MOv18+ ovarian tumor cell line), was also compared in the presence of a bispecific antibody OC/TR, anti-CD3 x MOv18). The stimulation by IL-2 + BMA030 induced approximately a twofold higher cytotoxic activity than IL-2-activated T cells. This could be related to the state of activation of effector cells stimulated by IL-2 + BMA030, since the phenotypic analysis showed an increased proportion of T cells expressing several activation/differentiation markers (CD25, HLA-DR, CD45R0, adhesion molecules). These findings could be applied to the design of therapeutic protocols using anti-CD3 x antitumoral bispecific antibodies. [less ▲]

Defeminising Keats

Conference (1996, July)

Tomographie a émission de positons dans l'évaluation de l'extension ganglionnaire intrathoracique du cancer bronchique non petites cellules. Etude préliminaire chez 30 patients

in Revue des Maladies Respiratoires (1996), 13(3), 281-6

Current methods for evaluating the mediastinum include chest radiography, computed tomography (CT) or magnetic resonance (MR) imaging and mediastinoscopy. Despite advances in morphologic imaging, some ... [more ▼]

Current methods for evaluating the mediastinum include chest radiography, computed tomography (CT) or magnetic resonance (MR) imaging and mediastinoscopy. Despite advances in morphologic imaging, some lung cancer patients are found to have unresectable disease at surgery. In contrast to CT scan or MR imaging, which depend primarily on anatomic and morphological criteria, positron emission tomography (PET) with 18fluorodeoxyglucose (FDG) depends mainly of the metabolic characteristics of a tissue for the diagnosis of disease. We perform a prospective study to compare FDG-PET and CT of the thorax in the presurgical assessment of the mediastinum in patients with newly diagnosed non-small cell lung cancer. Thirty patients have been included. CT and PET-scans were interpreted separately and results were compared to surgical staging during thoracotomy. In assessing mediastinal involvement, CT scan had a sensitivity of 56% and a specificity of 64%. For diagnosis mediastinal nodal disease, FDG-PET was 87% sensitive and 78% specific. Its positive predictive value was 82%, and the negative value was 83%. In conclusion, our preliminary results show that FDG-PET appears more accurate than CT in staging of mediastinal non-small cell lung cancer. [less ▲]

Vers un apprentissage expérientiel intégré des langues germaniques

Scientific conference (1996, June 24)

Un syndrome de Cushing d'origine inexpliquée

in Actualité Médicale Belge (1996), 513

Un homme de 25 ans est hospitalisé en août 1994 au CHU de Liége en vue de l'exploration de lombolgies chroniques et de manifestations cliniques d'hypercorticisme. Il présente en effet un visage boursouflé ... [more ▼]

Un homme de 25 ans est hospitalisé en août 1994 au CHU de Liége en vue de l'exploration de lombolgies chroniques et de manifestations cliniques d'hypercorticisme. Il présente en effet un visage boursouflé avec une érythrase discrète et une augmentation de poids (prise de 10 kg en quelques mois) à localisation tronculaire prédominante. Ces symptômes s'accompagnent d'une asthénie importante et de crampes musculaires au niveau des extrémités. [less ▲]

Etude du comportement du Contenu Electronique Total et de ses irrégularités dans une région de latitude moyenne. Application aux calculs de positions relatives par le GPS

Doctoral thesis (1996)

Actualités sur les marqueurs de l'infarctus du myocarde: choix, méthodes de mesure, interprétation

Conference (1996, June 13)

Mémoire de travail et langage chez les personnes retardées mentales : Nouvelles méthodologies

Conference (1996, June 08)

Automation en microbiologie: Actualité et perspectives

Conference (1996, June 05)

Chain end effects and dewetting in thin polymer films

in Macromolecules (1996), 29(12), 4305-4313

The dewetting of thin films of end-functionalized polymers, ω- and α,ω-barium sulfonato polystyrenes, on a silicon substrate has been investigated as a function of initial film thickness, molecular weight ... [more ▼]

The dewetting of thin films of end-functionalized polymers, ω- and α,ω-barium sulfonato polystyrenes, on a silicon substrate has been investigated as a function of initial film thickness, molecular weight, and functionality of the chains. The lower molecular weight monofunctional chains are found to dewet the substrate analogously to normal polystyrene but display an anomalous flow behavior at the surface. Moreover, after dewetting, the entire silicon surface still remains covered by a monolayer of monofunctional chains. The monolayer consists of a polymer brush of densely packed tethered chains, adsorbed via their ionic end groups. The dense packing and special conformations of the chains in the brush prevent interpenetration with other polymer chains, and the unadsorbed macromolecules dewet the brush. When the molecular weight of the monofunctional chains is increased, entanglements between the adsorbed polymer brush and the unadsorbed chains can occur and the dewetting process is retarded. Thin films of the difunctional chains do not dewet regardless of the molecular weight of the chains. The difference between mono- and difunctional materials is attributed to ionic aggregation, which is responsible for thermoreversible cross-linking and stabilization of thicker films by interaction of aggregates with dangling ends. It is suggested to use high molecular weight end-functionalized chains as polymeric additives to retard thin polymer film dewetting. [less ▲]