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See detailCrystallization and preliminary X-ray diffraction studies of a-amylase from the antarctic psychrophile Alteromonas haloplanctis A23
Aghajari, N.; Feller, Georges ULg; Gerday, Charles ULg et al

in Protein Science : A Publication of the Protein Society (1996), 5(10), 2128-2129

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the ... [more ▼]

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-Amylase from A. haloplanctis, which shares 53% sequence identity with pig pancreatic alpha-amylase, has been crystallized and data to 1.85 A have been collected. The space group is found to be C222(1) with a = 71.40 A, b = 138.88 A, and c = 115.66 A. Until now, a three-dimensional structure of a psychrophilic enzyme is lacking. [less ▲]

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See detailCandidatures : bis repetita non placent ?
Durand, Pascal ULg

Article for general public (1996)

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See detailThe Eye of the Storm: Janet Frame
Delrez, Marc ULg

Conference (1996)

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See detailPotential doubling time determination in a multicentre clinical study.
COUCKE, Philippe ULg

in Abstract book (1996)

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See detailStandard Abundances
Grevesse, Nicolas ULg; Noels-Grötsch, Arlette ULg; Sauval, A. J. et al

in Holt, Stephen S.; Sonneborn, George (Eds.) Proceedings of the sixth (6th) annual October Astrophysics Conference in College Park; Maryland; 9-11 October 1995 (1996)

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See detailPréface de L'archéologie, ses techniques
Longrée, Dominique ULg; Gillet, Joël

in Breyer, Catherine (Ed.) L'archéologie, ses techniques (1996)

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See detailOne-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.
Hokke, Cornelis H; Zervosen, Astrid ULg; Elling, Lothar et al

in Glycoconjugate Journal (1996), 13(4), 687-92

The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely ... [more ▼]

The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransferase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was formed in a 2.2 mumol ml-1 yield starting from the acceptor GlcNAc beta 1-->O-(CH2)8COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which pays an important role in various biological and immunological processes. [less ▲]

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See detailHeat Shock Proteins ans antioxidative enzymes in myocardial protection
Currie, R.W.; Plumier, Jean-Christophe ULg

in Knowlton, A.A. (Ed.) Heat Shock Proteins and the Cardiovascular System (1996)

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See detailPhotosensitized generation of hydroxyl radical by eught new sulfur and selenium analogs of psoralen
Collet, A.; Hoebeke, Maryse ULg; Piette, J. et al

in Journal of Photochemistry and Photobiology B : Biology (1996), 35

comparative study by ESR of the hydroxyl radical generation by 8 eight new sulfur and selenium analogs of psoralen

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See detailLes cyber-taureaux des vétérinaires liégeois
Durand, Pascal ULg

Article for general public (1996)

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See detail« Le diplôme n'est plus un passeport pour l'emploi »
Durand, Pascal ULg

Article for general public (1996)

Interview de l'économiste Jacques Lesourne, à l'occasion de la remise de ses insignes de docteur honoris causa de l'ULg

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See detailRéactions physiologiques face à des conditions extrêmes de chaleur et d'humidité relative. Etude de la récupération après l'exercice
Art, Tatiana ULg; Votion, Dominique ULg; Lekeux, Pierre ULg

in Pratique Vétérinaire Equine (1996), 28

The purpose of this study was to follow the recovery of horses during 1hour after treadmill exercise performed either in temperated atmospheric conditions (TC) (ambient temperature: 15°C; relative ... [more ▼]

The purpose of this study was to follow the recovery of horses during 1hour after treadmill exercise performed either in temperated atmospheric conditions (TC) (ambient temperature: 15°C; relative humidity: 55%) or in hot and humid conditions (HHC) (ambient temperature: 30°C; relative humidity: 75%). Five healthy fit standardbred horses (493± 14 kg; 4± 0,5 yrs) were investigated twice at eight days interval, in TC and in HHC. Some ventilatory and cardiorespiratory measurements were made during a standardised treadmill exercise test (SET), other measurements, i.e. cardiac and respiratory frequencies and rectal temperature, during a 1-hour recovery period. The SET consisted in 8 min warm-up and in 8 min exercise (1 min at 1,7, 4, 7, 8, 9 and 10 m/sec and 2 min at 4 m/sec with a 6% (3.4°) slope). Venous blond was sampled before, 2 min, 60 min and 48 hours after the completion of the test for biochemical analysis, namely plasma electrolytes and enzymatic activities. Exercising in HHC induced a reduction of the aerobic metabolism to the total energy requierement, i.e. a 20 % decrease of VO, and 55 % increase in lactate, as well as a decrease in VE. The increase in rectal and skip temperature, the dehydration and the weight loss were higher in HHC than in TC. During the recovery period the HR and f remained higher up to 30 minutes after the end of the SET in HHC while the rectal temperature remained higher up to 60 min after the end of this test. Sa, despite the fact that our horses recovered in TC after exercising in HHC, there were significant differences between their physiological parameters measured during the recovery. This means that the recovery in air-conditionned unit would probably be an insufficient measure to ensure an adequate cooling of the horses and justify consequently the adaptations proposed by the FEI, i.e. the decrease of the distance of the run and the increase of the lime devoted to the hall between phases [less ▲]

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See detailPolar overdominance at the ovine callipyge locus
Cockett, N.; Jackson, S.; Shaw, T. et al

in Science (1996), 273(5272), 236-238

An inheritable muscular hypertrophy was recently described in sheep and shown to be determined by the callipyge gene mapped to ovine chromosome 18. Here, the callipyge phenotype was found to be ... [more ▼]

An inheritable muscular hypertrophy was recently described in sheep and shown to be determined by the callipyge gene mapped to ovine chromosome 18. Here, the callipyge phenotype was found to be characterized by a nonmendelian inheritance pattern, referred to as polar overdominance, where only heterozygous individuals having inherited the callipyge mutation from their sire express the phenotype. The possible role of parental imprinting in the determinism of polar overdominance is envisaged. [less ▲]

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See detailL'Europe : un espoir à cultiver
Durand, Pascal ULg

Article for general public (1996)

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See detailFirst results on the development of an in ovulo culture technique of globular embryo in Phaseolus
Dherte, O; Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg

in Belgian Plant Tissue Culture Group (1996)

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See detailAg-NORs proteins from isolated nucleoli of Ehrlich tumour cells
Vandelaer, M; Thiry, Marc ULg; Goessens, G

Poster (1996)

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See detailLe cheval de trait ardennais convient-il pour l'attelage sportif
Crigel, MH; Art, Tatiana ULg; Votion, Dominique ULg et al

in Pratique Vétérinaire Equine (1996), 28

The Ardennais draught horse (DH) is more and more used for harnessing competitions. The aim of this study was to improve our knowledge of its cardiorespiratory peculiarities, both at rest and during ... [more ▼]

The Ardennais draught horse (DH) is more and more used for harnessing competitions. The aim of this study was to improve our knowledge of its cardiorespiratory peculiarities, both at rest and during exercise. At rest, arterial blood gas analysis, venous blood analysis, a test of mechanics of breathing and an endoscopy of the upper airways were performed. Experiments during exercise were performed on a treadmill. The intensity of the standardised test was sufficient to overshoot the OBLA threshold. The test was of increasing intensity and lasted 22 min. The cardiorespiratory parameters were measured during the last 5 min. Expired volume minute (VE), VT and f were measured. Respiratory O2 and CO2 fraction were measured with a mass spectrometer. Oxygen uptake (VOA, CO2 output (VCO2) and VE/VO2 were calculated. Heart rate (HR) was continuously measured.The results were compared to data recorded in standarbred horses (SH) during a treadmill exercise test leading to a similar lactaci¬demia (LA) and peak HR.The DH have a significantly lower V02 than the SH while running at an exercise intensity inducing the same HR and LA modifications. The lower aerobic capacity of DH was at least partly correlated with their higher cost of breathing and lower blood gas transport capacity.Moreover, other measurements have been performed infield conditions, during 4 draft competitions, i.e. speed, maniability, power and endurance. Venous blood analyses before and after the competition, heart rate and body temperature measurements were performed. The results of this second set of measurements confirm the results obtained in the laboratory and allow assessing that these kinds of competition do not induce dramatic physiological modifications and therefore respect the welfare and limits of these horses. [less ▲]

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See detailBrain Levels of Thiamine and Its Phosphate Esters in Friedreich's Ataxia and Spinocerebellar Ataxia Type 1
Bettendorff, Lucien ULg; Mastrogiacomo, Frank; LaMarche, J. et al

in Movement Disorders : Official Journal of the Movement Disorder Society (1996), 11(4), 437-439

Decreased blood and cerebrospinal fluid levels of thiamine have been reported in patients with spinocerebellar ataxia disorders. To determine whether a thiamine deficiency is present in the brain, we ... [more ▼]

Decreased blood and cerebrospinal fluid levels of thiamine have been reported in patients with spinocerebellar ataxia disorders. To determine whether a thiamine deficiency is present in the brain, we measured levels of thiamine and its phosphate esters thiamine monophosphate (TMP) and thiamine diphosphate (TDP), in postmortem cerebellar and cerebral cortices of patients with Friedreich's ataxia (FA) and spinocerebellar ataxia type 1 (SCA1). Brain levels of free (nonphosphorylated) thiamine, TMP, TDP, and total thiamine in FA and SCA1 were, on average, not significantly different from control values. However, a nonsignificant trend was observed for slightly reduced levels of TDP and total thiamine in cerebellar cortex of the SCA1 patients, a finding that might be related to the severe neuronal damage in this brain area. We conclude that in FA, brain thiamine concentrations are normal, whereas in SCA1 the levels are, at most, only slightly reduced. [less ▲]

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