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See detailLocalization of Testosterone-Sensitive and Sexually Dimorphic Aromatase-Immunoreactive Cells in the Quail Preoptic Area
Balthazart, Jacques ULg; Tlemcani, O.; Harada, N.

in Journal of Chemical Neuroanatomy (1996), 11(3), 147-71

The distribution of aromatase-immunoreactive cells was studied in the medial preoptic nucleus of male and female quail that were sexually mature and gonadally intact, or gonadectomized, or gonadectomized ... [more ▼]

The distribution of aromatase-immunoreactive cells was studied in the medial preoptic nucleus of male and female quail that were sexually mature and gonadally intact, or gonadectomized, or gonadectomized and treated with testosterone. The study first confirmed the existence of a significant difference in the number of aromatase-immunoreactive cells between males and females (males > females) and the marked effect of castration and testosterone treatment which, respectively, decrease and restore the number of these cells. An analysis of the distribution in space of this neurochemically defined cell population was also carried out. This study revealed that castration does not uniformly decrease the density of aromatase-immunoreactive cells, but local increases are observed in an area directly adjacent to the third ventricle. A number of new sex differences in the organization of the medial preoptic nucleus and its population of aromatase cells have, in addition, been identified. The density of aromatase-immunoreactive cells is not higher in males than in females throughout the nucleus, but a higher density of immunoreactive cells is present in the ventromedial part of the nucleus in females as compared to males. In addition, the cross-sectional area of the nucleus as defined by the population of aromatase-immunoreactive cells is larger in males than in females in its rostral part and its shape is more elongated in the dorso-ventral direction in females than in males. Some of these differences (e.g. higher density of ARC-ir cells in the ventromedial part of the female POM, shape of the nucleus) appear to be organizational in nature, because they are still present in birds exposed to the same endocrine conditions during adult life (e.g. gonadectomized and treated with a same dose of testosterone). This conclusion should now be tested by experiments manipulating the endocrine environment of quail embryos. The anatomical heterogeneity of the medial preoptic nucleus revealed by this study also suggests a functional heterogeneity and the specific roles of the medial and lateral parts of the nucleus should also be investigated. [less ▲]

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See detailLes processus expansifs endosellaires non adénomateux
Beckers, Albert ULg

Scientific conference (1996, September)

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See detailThe non-penicillin-binding module of the tripartite penicillin-binding protein 3 of Escherichia Coli is required for folding and/or stability of the penicillin-binding module and the membrane-anchoring module confers cell septation activity on the folded structure
Goffin, Colette ULg; Fraipont, Claudine ULg; Ayala, Juan et al

in Journal of Bacteriology (1996), 178(18), 5402-5409

The ftsI-encoded multimodular class B penicillin-binding protein 3 (PBP3) is a key element of the cell septation machinery of Escherichia coli. Altered ftsI genes were overexpressed, and the gene products ... [more ▼]

The ftsI-encoded multimodular class B penicillin-binding protein 3 (PBP3) is a key element of the cell septation machinery of Escherichia coli. Altered ftsI genes were overexpressed, and the gene products were analyzed with respect to the level of production, stability, penicillin affinity, and cell septation activity. In contrast to the serine beta-lactamases and low-molecular-mass PBPs which are autonomous folding entities, the S-259-to-V-577 penicillin-binding module of M-1-to-V-577 PBP3 lacks the amino acid sequence information for correct folding. The missing piece of information is provided by the associated G-57-to-E-258 non-penicillin-binding module which functions as a noncleaved, pseudointramolecular chaperone. Key elements of the folding information reside within the motif 1-containing R-60-to-W-110 polypeptide segment and within G-188-to-D-197 motif 3 of the n-PB module. The intermodule interaction is discussed in the light of the known three-dimensional structure (at 3.5-A [0.35-nm] resolution) of the analogous class B PBP2x of Streptococcus pneumoniae (S. Pares, N. Mouz, Y. Petillot, R. Hakenbeck, and O. Dideberg, Nature Struct. Biol. 3:284-289, 1996). Correct folding and adoption of a stable penicillin-binding conformation are necessary but not sufficient to confer cell septation activity to PBP3 in exponentially growing cells. The in vivo activity of PBP3 also depends on the M-1-to-E-56 amino-terminal module which encompasses the cytosol, the membrane, and the periplasm and which functions as a noncleaved pseudo-signal peptide. [less ▲]

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See detailDevelopment of an integrated tool for solar buildings design and renewal.
Andre, Philippe ULg; Nicolas, Jacques ULg

in Proceedings PLEA 96 International conference (1996, September)

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See detailDevelopment of simplified models for solar buildings optimal control
Kummert, M.; Andre, Philippe ULg; Nicolas, Jacques ULg

in Proceedings EuroSun 96 (1996, September)

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See detailE-LOTOS Core Language
Jeffrey, Alan; Leduc, Guy ULg

Report (1996)

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See detailShip resistance and propulsion
Marchal, Jean ULg

Learning material (1996)

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See detailNaevocyte Triggering by Recombinant Human Growth Hormone
Pierard, Gérald ULg; Pierard-Franchimont, Claudine ULg; Nikkels, Arjen ULg et al

in Journal of Pathology (The) (1996), 180(1), 74-79

The influence of growth hormone and insulin-like growth factor I on human melanocytes is being increasingly recognized. Clinical evidence has shown that when recombinant human growth hormone (hGH) is ... [more ▼]

The influence of growth hormone and insulin-like growth factor I on human melanocytes is being increasingly recognized. Clinical evidence has shown that when recombinant human growth hormone (hGH) is administered to children of short stature, the growth of melanocytic naevi is boosted. This study was conducted on 56 hGH-triggered naevi and nine similar lesions excised before or after hGH therapy for hypopituitarism and Turner's syndrome. A series of 40 naevi excised from age-matched healthy children served as controls. Atypicality of naevocytes was investigated using image analysis, AgNOR counts, immunohistochemistry (HMB-45, NKI-C3, Ki-67, anti-bcl-2-oncoprotein), and DNA flow cytometry. The data associate hGH treatment with anisokaryosis and increased AgNOR and Ki-67 counts in naevocytes. The same cells also show abnormal patterns of HMB-45 immunolabelling. These indications of naevocyte activation were not suggestive of malignant transformation. hGH-triggered melanocytomas should be added to the list of atypical melanocytic naevi. The long-term evolution of these lesions remains unknown and the potential risk of malignant transformation awaits careful evaluation. [less ▲]

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See detailDevelopment of a cost calculation module for the inland and sea-river waterway transport
Marchal, Jean ULg; Kicheva, D.

in Proceedings of the PIANC Conference on Inland and Maritime navigation and Coastal Problems of East European Countries (1996, September)

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See detailShip resitance and flow
Marchal, Jean ULg; Bulgarelli, U.; Jensen, P. S. et al

in Proceedings of the 21st ITTC Conference (1996, September), volume 1

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See detailFundamental definitions of ship and its stability
Marchal, Jean ULg

Learning material (1996)

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See detailEvaluation of ribotyping versus pulsed-field gel electrophoresis for epidemiologic typing of Pseudomonas pickettii
Chetoui, Hafid; MELIN, Pierrette ULg; Struelens, M. J. et al

Poster (1996, September)

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See detailEvaluation of Etest for susceptibility testing of Mycobacterium tuberculosis and mycobacteria other than tuberculosis
MELIN, Pierrette ULg; Dirickx, Corine; Godefroid, Olivier et al

Conference (1996, September)

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See detailProtection du citoyen et expérimentation médicale
Palermini, Patricia ULg

Report (1996)

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See detailResponses of six coniferous forest soils to increased (NH4)2SO4 inputs
Carnol, Monique ULg; Ineson, Phil

Conference (1996, September)

Defining Critical N Loads for forests remains a major challenge, as our understanding of processes determining forest ecosystem responses to increased N deposition is still poor. Acidification will mainly ... [more ▼]

Defining Critical N Loads for forests remains a major challenge, as our understanding of processes determining forest ecosystem responses to increased N deposition is still poor. Acidification will mainly depend on the nitrification capacity, and coupled nitrate leaching, of the forest soil; yet factors controlling nitrification are not clearly established. As part of the CORE project (CEC), investigating nutrient dynamics in European coniferous forest soils, we studied the effects of increased (NH4)2SO4 deposition on soil solution chemistry at six sites situated across a climatic and pollution gradient. Roofed tension lysimeters were treated every two weeks with 2.88 kg N ha-1 (75 kg N ha-1 a-1) above ambient throughfall and monitored at the same interval for a total of 18 months. Responses to increased (NH4)2SO4 inputs varied from high ammonium retention, to highly increased ammonium leaching at the different sites. The important links between N cycle transformations and cation leaching were confirmed. The response of the nitrifiers to increased external ammonium supply was slow at all sites. The different responses of these six soils to increased (NH4)2SO4 deposition emphasizes that the effects of N loads have to be discussed in relation to soil N storage and nitrification capacity. [less ▲]

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See detailFeeding of Sarpa salpa in a Posidonia seagrass ecosystem: del 13C evidence for a mixed diet
Havelange, Stéphane; Lepoint, Gilles ULg; Dauby, Patrick ULg et al

Conference (1996, September)

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See detailAerosol therapy in the equine species
Votion, Dominique ULg; Duvivier, Hannia; Lekeux, Pierre ULg

in In Proceedings: Journées Vétérinaires Suisses (1996, September)

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See detailModel-Based Ladar ATR Using Functional Templates
Verly, Jacques ULg

Conference (1996, September)

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