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See detailCodon adjustment to maximise heterologous gene expression in Streptomyces lividans can lead to decreased mRNA stability and protein yield.
Lammertyn, E; Van Mellaert, L; Bijnens, A P et al

in Molecular & General Genetics [=MGG] (1996), 250(2), 223-9

The impact of the codon bias of the mouse tumour necrosis factor alpha (mTNF) gene cloned in Streptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the ... [more ▼]

The impact of the codon bias of the mouse tumour necrosis factor alpha (mTNF) gene cloned in Streptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the mTNF gene were therefore adapted to the codon bias of Streptomyces by site-directed mutagenesis. No improvement in mTNF yield could be detected. The stability of the transcript derived from the construct was shown to be more important for determining the final level of mTNF production. A strong correlation was observed between the yield of secreted biologically active mTNF and the amount of mTNF mRNA present in the cells. [less ▲]

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See detailUse of the monofrequency forced oscillation technique to measure changes in upper airway resistance in Friesian and blue Belgian calves
Reinhold, Petra; Genicot, Bruno; Close, Roland et al

in Research in Veterinary Science (1996), 61(3), 227-230

Monofrequency forced oscillometry (MFO) using a sinusoidal test signal of one frequency (10 Hz) was compared with the classical pulmonary function technique (using an oesophageal balloon with airflow ... [more ▼]

Monofrequency forced oscillometry (MFO) using a sinusoidal test signal of one frequency (10 Hz) was compared with the classical pulmonary function technique (using an oesophageal balloon with airflow measurements) in two breeds of cattle which differ in their respiratory physiology, particularly to assess changes in upper airway resistance. Six healthy male Friesian calves (mean [SD] bodyweight 131 [12] kg) and six healthy male blue Belgian calves (mean [SD] bodyweight 137 [13] kg) were examined successively with their heads either in a normal position or held vertically, using a cross-over study design. In the normal position, higher resistances, a higher respiratory impedance, and lower values of the dynamic lung compliance (Cdyn) were measured in the blue Belgian than in the Friesian calves. In the vertical head position, total pulmonary resistance (RL) increased and Cdyn decreased significantly (P < 0.05). The changes in MFO-derived parameters were in accordance with the changes in classical parameters, and for both methods, the percentage changes were markedly higher in the blue Belgian calves. [less ▲]

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See detailA Salute to Malaco Records - Interview with Tommy Couch
Sacré, Robert ULg

Article for general public (1996)

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See detailL'entreprise d'insertion en Wallonie: premières leçons de 7 projets-pilotes
Defourny, Jacques ULg; Van Pachterbeke, Isabelle; Simon, Michel

Book published by Fondation Roi Baudouin (1996)

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See detailWolf-Rayet stars in the framework of stellar evolution.
Vreux, Jean-Marie ULg; Detal, Alain ULg; Fraipont-Caro, D. et al

Book (1996)

The following topics were dealt with: evolution of massive stars - theory; observational characteristics of Wolf-Rayet stars and related objects; evidences for evolutionary links - transition objects ... [more ▼]

The following topics were dealt with: evolution of massive stars - theory; observational characteristics of Wolf-Rayet stars and related objects; evidences for evolutionary links - transition objects; analysis of the observations - the models and their results; massive stars in starburst. [less ▲]

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See detailThe pivotal role of cross-calibration and quality control in an international clinical trial of the strontium salt S-12911 involving multiple DXA brands
Slosman, DO; Meunier, PJ; Delmas, PD et al

in Osteoporosis International (1996), 6(S1), 168

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See detailA Tribute to Dave Clark
Sacré, Robert ULg

Article for general public (1996)

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See detailThe nucleolus during the cell cycle.
Thiry, Marc ULg; Goessens, Guy ULg

Book published by R.G. Landes Company, Chapman and Hall (1996)

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See detailImprint and Brush Cytology in the Diagnosis of Canine Intranasal Tumours
Clercx, Cécile ULg; Wallon, J.; Gilbert, S. et al

in Journal of Small Animal Practice (1996), 37(9), 423-7

Fifty-four dogs with nasal tumours were included in this study. Based on histopathology 52 tumours were malignant (36 epithelial and 16 mesenchymal) and two were benign (one oncocytoma and one ... [more ▼]

Fifty-four dogs with nasal tumours were included in this study. Based on histopathology 52 tumours were malignant (36 epithelial and 16 mesenchymal) and two were benign (one oncocytoma and one pleiomorphic adenoma). Malignancy was significantly more frequently diagnosed by imprint cytology (81 per cent of the cases) than by brush cytology (56 per cent). Brush cytology was a significantly more sensitive technique in epithelial than in mesenchymal tumours, while the sensitivity of imprint cytology was not affected by the histological type. Brush cytology determined an epithelial origin in 88 per cent of epithelial tumours, and imprint cytology in 90 per cent. In mesenchymal tumours, the scores were significantly lower, the histological type being determined in only 20 per cent and 50 per cent, using brush and imprint cytology, respectively. [less ▲]

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See detailLes chemins de la compréhension : brochure d'accompagnement au film éponyme de Terwagne et Widart
Terwagne, Serge; Widart, Nicole; Lafontaine, Dominique ULg

Report (1996)

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See detailCabergoline, a new long-acting dopamine agonist, in the treatment of acromegaly
Abs, R.; Verhelst, J.; Verbessem, G. et al

in 10th international Congress of Endocrinology - Abstract book (1996)

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See detailDifférenciatio, génétique et morphologique du mulot, Apodemus sylvaticus, dans le bassin méditerranéen occidental
Michaux, J.R.; Libois, Roland ULg; Fons, Roger

in Vie et milieu (1996), 46(3-4), 192-203

Many questions are still unanswered about the biogeography and the origin of the wood mouse in the Mediterranean area, namely on the islands where some giant forms are described. In order to characterize ... [more ▼]

Many questions are still unanswered about the biogeography and the origin of the wood mouse in the Mediterranean area, namely on the islands where some giant forms are described. In order to characterize the genetic structure of these insular populations and the level of morphological differentiation, 232 animals originating from 30 localities situatid on several wester Mediterranean islands and on the continent, either along the sea coast or more inlabd, were trapped. Their skulls were measured and their mtDNA purified and analysed by RFLP techniques. The wood mice living on small islands are generally bigger than their relatives living on the continent whereas the size of those living on larger islands is quite the same. This kind of insular gigantism ought to be more an adaptive response to peculiar insular environmental conditions (particularly a lower predation pressure) than a consequence of a founder effect. The mtDNA of the wood mice from north eastern Spain belongs to the previously identified northwestern group. Consequently, the Pyrenees are not a biogeographic barrier to the wood mouse. The Sicilian mice, which are genetically different from all the other west European or north African animals, are morphologically similar to the Sardinia and Italian ones. [less ▲]

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See detailEnrico Mattioda, Teorie della tragedia nel settecento, Modena, Mucchi, 1994, 366
Curreri, Luciano ULg

in Franco-Italica (1996), 9

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See detailLa classification numérique : principes et applications
Palm, Rodolphe ULg

in Notes de Statistique et d'Informatique (1996), (1), 28

The principles of some clustering techniques are described and illustrated by an example. The data consist of chemical contents for 45 types of mineral water.

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See detailGuide bibliographique pour l'histoire de la principauté de Liège au 18e siècle : La vie intellectuelle
Droixhe, Daniel ULg; Vanwelkenhuyzen, Nadine ULg

in Annuaire d'Histoire Liégeoise (1996), Numéro spécial

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See detailDeffand, Marie de Vichy-Chamrond, marquise du
Tilkin, Françoise ULg

in Voir barré (1996), 12-13

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