References of "1995"
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See detailComment évaluer la sécrétion insulinique en pratique?
Scheen, A. J.; Paquot, Nicolas ULg; Letiexhe, M. R. et al

in Diabète & Métabolisme (1995), 21(6), 458-64

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See detailLa passion du philosophe
Durand, Pascal ULg

in Avancées (1995)

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See detailCécité et fantastique dans rapport sur les aveugles d'Alberto Breccia
Denis, Benoît ULg; Ghizzardi, Yves

in Voir (1995), (11), 52-63

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See detailThe Beta-Lactamase Secreted by the Antarctic Psychrophile Psychrobacter Immobilis A8
Feller, Georges ULg; Sonnet, P.; Gerday, Charles ULg

in Applied and Environmental Microbiology (1995), 61(12), 4474-6

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal ... [more ▼]

A class C beta-lactamase has been purified from the culture supernatant of the antarctic psychrophile Psychrobacter immobilis A8. This psychrophilic beta-lactamase displays a low level of thermal stability and a low optimal temperature of activity. In contrast to other cold-adapted enzymes, its level of specific activity is not higher than that of mesophilic class C beta-lactamases. [less ▲]

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See detailA Wedding Blues Party in the Windy City
Sacré, Robert ULg

Article for general public (1995)

Fête du mariage de Bruce Iglauer (Alligator Records) avec Jo Kolanda au Buddy Guy's Legends Club de Chicago avec une pléiade de musiciens de blues

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See detailLaisse le Bon Temps Rouler - Zydeco & Cajun Music Heat in Louisiana
Sacré, Robert ULg

Article for general public (1995)

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See detailWhich fault ruptured in the main (Ms = 6.7) shock of the 1981 Corinth earthquake sequence?
Hubert, Aurelia ULg; King; Armijo, R et al

in EOS (American Geosciences Union abstract) (1995, December), 76

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See detailPretherapeutic assessment of cerebral aneuvrysms with arterially enhanced spiral CT
Vanderschelden, P.; Flandroy, P.; Dondelinger, Robert ULg et al

Conference (1995, November 26)

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See detailCo-registration and complex interpolation
De Rauw, Dominique ULg; Roose, Stéphane ULg

in Franceschetti, G.; Oliver, C.; Shiue, J. (Eds.) et al Synthetic Aperture Radar and Passive Microwave Sensing (1995, November 25)

This paper presents a novel and efficient method, based on a modified version of the Chirp-Z transform algorithm, for performing the co-registration of SAR images as required in SAR interferometry. The ... [more ▼]

This paper presents a novel and efficient method, based on a modified version of the Chirp-Z transform algorithm, for performing the co-registration of SAR images as required in SAR interferometry. The method also allows complex interpolation of an entire image, by application, at each point of a bilinear coordinate transformation. [less ▲]

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See detailBeef production in Belgium: to which extent manage extensification
Istasse, Louis ULg; Clinquart, Antoine ULg; Dufrasne, Isabelle ULg et al

in Proceeding of a workshop held in Paris on Novembre 22, 23 and 24, 1995 (1995)

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See detailApproches nouvelles en matière de qualité des fourrages.
Lecomte, Ph.; Agneessens, R.; Dardenne, P. et al

Conference given outside the academic context (1995)

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See detailLa relation entre l'enseignement et le monde du travail face à de nouveaux défis
Robert, Jocelyne ULg

Conference (1995, November 16)

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See detailGenetic Mapping of Mitochondrial Markers by Recombinational Analysis in Chlamydomonas Reinhardtii
Remacle, Claire ULg; Colin, Martine ULg; Matagne, René-Fernand ULg

in Molecular & General Genetics [=MGG] (1995), 249(2), 185-90

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type ... [more ▼]

The mitochondrial genome of Chlamydomonas reinhardtii is a 15.8 kb linear DNA molecule present in multiple copies. In crosses, the meiotic products only inherit the mitochondrial genome of the mating type minus (paternal) parent. In contrast mitotic zygotes transmit maternal and paternal mitochondrial DNA copies to their diploid progeny and recombinational events between molecules of both origins frequently occur. Six mitochondrial mutants unable to grow in the dark (dk- mutants) were crossed in various combinations and the percentages of wild-type dk+ recombinants were determined in mitotic zygotes when all progeny cells had become homoplasmic for the mitochondrial genome. In crosses between strains mutated in the COB (apocytochrome b) gene and strains mutated in the COX1 (subunit 1 of cytochrome oxidase) gene, the frequency of recombination was 13.7% (+/- 3.2%). The corresponding physical distance between the mutation sites was 4.3 kb. In crosses between strains carrying mutations separated by about 20 bp, a recombinational frequency of 0.04% (+/- 0.02%) was found. Two other mutants not yet characterized at the molecular level were also used for recombinational studies. From these data, a linear genetic map of the mitochondrial genome could be drawn. This map is consistent with the positions of the mutation sites on the mitochondrial DNA molecule and thereby validates the method used to generate the map. The frequency of recombination per physical distance unit (3.2% +/- 0.7% per kilobase) is compared with those obtained for other organellar genomes in yeasts and Chlamydomonas. [less ▲]

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See detailMutations Affecting the Mitochondrial Genes Encoding the Cytochrome Oxidase Subunit I and Apocytochrome B of Chlamydomonas Reinhardtii
Colin, Martine ULg; Dorthu, M. P.; Duby, Franceline ULg et al

in Molecular & General Genetics [=MGG] (1995), 249(2), 179-84

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase ... [more ▼]

Mitochondrial mutants of the green alga Chlamydomonas reinhardtii that are inactivated in the cytochrome pathway of respiration have previously been isolated. Despite the fact that the alternative oxidase pathway is still active the mutants have lost the capacity to grow heterotrophically (dark + acetate) and display reduced growth under mixotrophic conditions (light + acetate). In crosses between wild-type and mutant cells, the meiotic progeny only inherit the character transmitted by the mt- parent, which indicates that the mutations are located in the 15.8 kb linear mitochondrial genome. Two new mutants (dum-18 and dum-19) have now been isolated and characterized genetically, biochemically and at the molecular level. In addition, two previously isolated mutants (dum-11 and dum-15) were characterized in more detail. dum-11 contains two types of deleted mitochondrial DNA molecules: 15.1 kb monomers lacking the subterminal part of the genome, downstream of codon 147 of the apocytochrome b (COB) gene, and dimers resulting from head-to-head fusion of asymmetrically deleted monomers (15.1 and 9.5 kb DNA molecules, respectively). As in the wild type, the three other mutants contain only 15.8 kb mitochondrial DNA molecules. dum-15 is mutated at codon 140 of the COB gene, a serine (TCT) being changed into a tyrosine (TAC). dum-18 and dum-19 both inactivate cytochrome c oxidase, as a result of frameshift mutations (addition or deletion of 1 bp) at codons 145 and 152, respectively, of the COX1 gene encoding subunit I of cytochrome c oxidase. In a total of ten respiratory deficient mitochondrial mutants characterized thus far, only mutations located in COB or COX1 have been isolated.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailLa pédologie n'offre-t'elle qu'un profil aux horizons réduits ?
Bock, Laurent ULg

Conference (1995, November 15)

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See detailMacromolecular engineering of polylactones and polylactides. 20. Effect of monomer, solvent, and initiator on the ring-opening polymerization as initiated with aluminum alkoxides
Ropson, Nathalie; Dubois, Philippe ULg; Jérôme, Robert ULg et al

in Macromolecules (1995), 28(23), 7589-7598

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization ... [more ▼]

Structure and coordinative association of the propagating species have been studied by H-1, Al-27, and C-13 NMR and viscosimetry in case of epsilon-caprolactone (E-CL) and lactides (LA) polymerization initiated by aluminum alkoxides. In toluene, aluminum triisopropoxide, a commonly used initiator, forms tetrameric (A(4)) and/or trimeric (A(3)) aggregates, which are dissociated upon the addition of the cyclic monomer. Actually, LA is responsible for the complete deaggregation of both A(3) and A(4) into single species (A(1)) trisolvated by the monomer (Al coordination number, CN = 6), whereas A(3) is selectively deaggregated by epsilon-CL. This behavior is consistent with the higher stability of A(4) compared to A(3). Whatever the monomer, three chains are initiated by the active A(1) species. When conversion of epsilon-CL is complete, the active aluminum trialkoxide sites are solvated by one (CN = 4) and more probably three (CN = 6) carbonyl groups of the attached poly-epsilon-caprolactone chains. At the complete LA conversion, only one carbonyl group of the polylactide chains is coordinated to Al (CN = 4). In THF, there is a competition between monomer and solvent for coordination to Al, which explains why polymerization is slower in THF than in toluene. In both toluene and THF, diethylaluminum monoalkoxide forms cyclic trimers that are solvated by the monomer (epsilon-CL) at least on the NMR time scale. Nevertheless, a fractional kinetic order in initiator is observed which gives credit to a fast reversible dissociation of the epsilon-CL-solvated trimers. [less ▲]

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See detailThe Replication in Vitro of the Gammaherpesvirus Bovine Herpesvirus 4 Is Restricted by Its DNA Synthesis Dependence on the S Phase of the Cell Cycle
Vanderplasschen, Alain ULg; Goltz, M.; Lyaku, J. et al

in Virology (1995), 213(2), 328-40

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to ... [more ▼]

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to determine the relationship between BHV-4 infection and the cell cycle. The temporal expression of BHV-4 late (L) proteins in unsynchronized cell cultures was first investigated by flow cytometry. Interestingly, L protein expression occurred in a limited number of cells infected with a high multiplicity of infection, and a reciprocal correlation between the percentage of positive cells and the cell density at the time of infection was demonstrated. Moreover, the finding that a BHV-4 early-late protein was expressed in nearly all the cells suggested that a blockage in the viral replication cycle occurred in some infected cells at the stage of viral DNA synthesis or L protein expression. Because this blockage could be the consequence of the dependence of one or both of these events on the cell cycle, they were investigated after infection of synchronized cell cultures. The following findings were made. (i) Cell transition through the S phase quantitatively increased the rate of BHV-4 DNA replication. (ii) BHV-4 DNA synthesis could not be detected in cells arrested in G0. (iii) Synchronization of MDBK cells with Lovastatin before infection increased the percentage of cells expressing L proteins. (iv) In contrast, infection of cells arrested in G0 led to few positive cells. Taken together these results showed that BHV-4 DNA replication and consequently the expression of L proteins are dependent on the S phase of the cell cycle. This dependence could be of importance for several biological properties of BHV-4 infection in vitro and might have implications for the biology of the virus in vivo. [less ▲]

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