Effects of infused sodium lactate on glucose and energy metabolism in healthy humans.
Paquot, Nicolas ; ; et al
in Diabètes & Métabolism (1995), 21(5), 345-52
To assess the effects of lactate on glucose metabolism, sodium lactate (20 mumol.kg-1.min-1) was infused into healthy subjects in basal conditions and during application of a hyperinsulinaemic (6 pmol.kg ... [more ▼]
To assess the effects of lactate on glucose metabolism, sodium lactate (20 mumol.kg-1.min-1) was infused into healthy subjects in basal conditions and during application of a hyperinsulinaemic (6 pmol.kg-1.min-1) euglycaemic clamp. Glucose rate of appearance (GRa) and disappearance (GRd) were measured from plasma dilution of infused U- 13C glucose, and glucose oxidation (G(ox)) from breath 13CO2 and plasma 13C glucose. In basal conditions, lactate infusion did not alter G(ox) (8.8 +/- 0.9 vs 9.2 +/- 1.1 mumol.kg-1.min-1), while GRa slightly decreased from 15.2 +/- 0.8 basal to 13.9 +/- 0.9 mumol.kg-1.min-1 after lactate (p < 0.05). During a hyperinsulinaemic clamp, hepatic glucose production was completely suppressed with or without lactate. Lactate decreased G(ox) from 17.1 +/- 0.4 to 13.4 +/- 1.2 mumol.kg-1.min-1 (p < 0.05), whereas GRd was unchanged (39.7 +/- 3.6 vs 45.6 +/- 2.6 mumol.kg-1.min-1. It is concluded that infusion of lactate in basal conditions does not increase GRa or interfere with peripheral glucose oxidation, and that during hyperinsulinaemia lactate decreases glucose oxidation but does not alter hepatic or peripheral insulin sensitivity. [less ▲]Detailed reference viewed: 8 (2 ULg)
Etude des gommes arabiques et de leurs procédés de mise en oeuvre dans des émulsions contenant des arômes.
; ; Fauconnier, Marie-Laure et al
Report (1995)Detailed reference viewed: 5 (1 ULg)
The SEVEX project: Assessment and validation of the SEVEX project.
; Everbecq, Etienne ; Bourouag, Mohamed et al
in Harmonisation within Atmospheric Dispersion Modelling for Regulatory Purposes (1995)Detailed reference viewed: 12 (6 ULg)
Transgenic mice expressing the human heat shock protein 70 have improved post-ischemic myocardial recovery.
Plumier, Jean-Christophe ; ; et al
in Journal of Clinical Investigation (1995), 95(4), 1854-60
Heat shock treatment induces expression of several heat shock proteins and subsequent post-ischemic myocardial protection. Correlations exist between the degree of stress used to induce the heat shock ... [more ▼]
Heat shock treatment induces expression of several heat shock proteins and subsequent post-ischemic myocardial protection. Correlations exist between the degree of stress used to induce the heat shock proteins, the amount of the inducible heat shock protein 70 (HSP70) and the level of myocardial protection. The inducible HSP70 has also been shown to be protective in transfected myogenic cells. Here we examined the role of human inducible HSP70 in transgenic mouse hearts. Overexpression of the human HSP70 does not appear to affect normal protein synthesis or the stress response in transgenic mice compared with nontransgenic mice. After 30 min of ischemia, upon reperfusion, transgenic hearts versus nontransgenic hearts showed significantly improved recovery of contractile force (0.35 +/- 0.08 versus 0.16 +/- 0.05 g, respectively, P < 0.05), rate of contraction, and rate of relaxation. Creatine kinase, an indicator of cellular injury, was released at a high level (67.7 +/- 23.0 U/ml) upon reperfusion from nontransgenic hearts, but not transgenic hearts (1.6 +/- 0.8 U/ml). We conclude that high level constitutive expression of the human inducible HSP70 plays a direct role in the protection of the myocardium from ischemia and reperfusion injury. [less ▲]Detailed reference viewed: 13 (1 ULg)
Hydroxychloroquine (Plaquenil) for recurrence prevention of Crohn's disease after curative surgery.
Louis, Edouard ; Belaiche, Jacques
in Gastroentérologie Clinique et Biologique (1995), 19(2), 233-4Detailed reference viewed: 103 (1 ULg)
NF-kappaB transcription factor activation by hydrogen peroxide can be decreased by 2,3-dihydroxybenzoic acid and its ethylester derivative
; ; Legrand-Poels, Sylvie et al
in Archives of Biochemistry & Biophysics (1995)Detailed reference viewed: 3 (0 ULg)
Dendrochronologie en Belgique et régions limitrophes
Hoffsummer, Patrick ; Houbrechts, David
in Archaeologia mediaevalis (1995), 18(2), 3-4
This is a presentation of dendrochronological analyses of CEA (ULg) in 1994 in the following cities : Bouillon (B), Burg-Reuland (B), Gent (B), Havelange (B), Hotton (B), Laon (F), Liège (B), Luxembourg ... [more ▼]
This is a presentation of dendrochronological analyses of CEA (ULg) in 1994 in the following cities : Bouillon (B), Burg-Reuland (B), Gent (B), Havelange (B), Hotton (B), Laon (F), Liège (B), Luxembourg (GDL), Marloie (B), Revin (F), Soignies (B), Tongeren (B) and Verviers (B) [less ▲]Detailed reference viewed: 12 (2 ULg)
Compte rendu de Michel KAPLAN, Les hommes et la terre à Byzance du VIe au XIe siècle. Propriété et exploitation du sol, Paris, 1992
in Cahiers de Clio (1995)Detailed reference viewed: 5 (0 ULg)
Coexpression of cytokeratin and vimentin in eutopic endometrium and endometriosis throughout the menstrual cycle: evaluation by a computerized method.
NISOLLE, Michelle ; ;
in Fertility and Sterility (1995), 64(1), 69-75
Objectives: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: The investigation of cytokeratin and vimentin was ... [more ▼]
Objectives: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: The investigation of cytokeratin and vimentin was carried out using the new computerized technology of image analysis. SETTING: University Hospital, Department of Gynecology. PATIENTS: Biopsies were taken from patients undergoing a laparoscopy for infertility (29 endometrial biopsies and 31 biopsies of peritoneal endometriosis). None of them were treated. RESULTS: Cyclic variations of cytokeratin and vimentin staining were noted in eutopic endometrium. The cytokeratin and vimentin staining pattern consistently was lower in ectopic endometrium than in eutopic endometrium. CONCLUSIONS: Endometriotic epithelial and stromal cells undergo a complex program of differentiation giving them histochemical characteristics similar to those observed in endometrium. Such a concomitant expression of antigenicity indicates their close relationship with their mesodermal müllerian origin. [less ▲]Detailed reference viewed: 3 (0 ULg)
Rectovaginal septum, endometriosis or adenomyosis: laparoscopic management in a series of 231 patients.
; NISOLLE, Michelle ; et al
in Human Reproduction (1995), 10(3), 630-5
A series of 231 cases of deep-infiltrating endometriosis of the rectovaginal septum is presented. Laparoscopic procedures with excision of deep fibrotic endometriotic nodules were performed in all cases ... [more ▼]
A series of 231 cases of deep-infiltrating endometriosis of the rectovaginal septum is presented. Laparoscopic procedures with excision of deep fibrotic endometriotic nodules were performed in all cases. In three cases, the bowel lumen was entered. No other peri-operative complications were observed. Three cases of urinary retention were reported. The nodule resection resulted in considerable pain relief. Histologically, the rectovaginal nodule was similar to an adenomyoma. Indeed it was a circumscribed nodular aggregate of smooth muscle and endometrial glands. This form of disease must be considered as a specific disease which originates from the Mullerian rests present in the rectovaginal septum and we suggest that it be called 'rectovaginal adenomyosis'. [less ▲]Detailed reference viewed: 5 (0 ULg)
A magnetic resonance imaging approach for the diagnosis of a triplet cornual pregnancy.
; ; NISOLLE, Michelle et al
in Fertility and Sterility (1995), 64(5), 1029-31
Objectives: To describe a rare case of triplet cornual pregnancy after IVF-ET and to assess the role of magnetic resonance imaging (MRI) for early diagnosis. DESIGN: Case report. SETTING: Infertility and ... [more ▼]
Objectives: To describe a rare case of triplet cornual pregnancy after IVF-ET and to assess the role of magnetic resonance imaging (MRI) for early diagnosis. DESIGN: Case report. SETTING: Infertility and IVF Unit, in an university medical center. PATIENT: A 31-year-old healthy patient with a 3-year history of primary male infertility. INTERVENTION: Standard IVF-ET treatment cycle, using a GnRH-agonist (long protocol) and hMG for ovarian stimulation. RESULTS: A cornual triplet pregnancy is described with the approach for the diagnosis. Magnetic resonance imaging was complementary to endovaginal sonography for early diagnosis and permitted a timed conservative management. CONCLUSIONS: When transvaginal ultrasound findings did not provide an accurate location of the pregnancy, MRI was indicated to confirm the diagnosis. [less ▲]Detailed reference viewed: 3 (0 ULg)
NF-kappaB transcription factor and human immunodeficiency virus type 1 (HIV-1) are activated by methylene blue photosensitization
; Legrand-Poels, Sylvie ; et al
in European Journal of Biochemistry (1995)Detailed reference viewed: 4 (0 ULg)
Advanced laparoscopic surgery for the removal of rectovaginal septum endometriotic or adenomyotic nodules.
; NISOLLE, Michelle
in Bailliere's Clinical Obstetrics & Gynaecology (1995), 9(4), 769-74
In the pelvis, three different forms of endometriosis (Donnez et al, 1992) must be considered: (1) peritoneal, (2) ovarian, (3) rectovaginal septum. By evaluation of the mitotic activity and the stromal ... [more ▼]
In the pelvis, three different forms of endometriosis (Donnez et al, 1992) must be considered: (1) peritoneal, (2) ovarian, (3) rectovaginal septum. By evaluation of the mitotic activity and the stromal vascularization, we have recently suggested (Nisolle et al, 1993) that peritoneal red lesions were the most aggressive form of the disease and progress to the so-called typical or black lesion, which must be considered as an enclosed implant surrounded by fibrosis. This type of infiltration must be clearly differentiated from the rectovaginal endometriotic nodule. Koninckx (1993) recently described three types of deep-infiltrating endometriosis: deep-infiltrating endometriosis of type I is a rather large lesion in the peritoneal cavity, infiltrating conically with the deeper parts becoming progressively smaller. It has been suggested that this type of endometriosis is caused by infiltration. In type II lesions, the main feature is that bowel is retracted over the lesion which thus becomes deeply situated in the rectovaginal septum although not actually infiltrating it. Type III lesions are the deepest and most severe. They are spherically shaped, situated deep in the rectovaginal septum, often only visible as a small typical lesion at laparoscopy or often not visible at all. This lesion is often more palpable than visible and is acutely tender if the patient is examined at the time of menstruation, and gives rise to severe dyspareunia. In our experience there are two different types of 'deep-infiltrating endometriosis': 1. True deep-infiltrating endometriosis caused by the invasion of a very active peritoneal lesion deep in the retroperitoneal space. In cases of lateral peritoneal invasion, uterosacral ligaments can be involved as well as the anterior wall of the rectosigmoid bowel junction resulting in a retraction, adhesions and secondary obliteration of the cul-de-sac. 2. Pseudo deep-infiltrating endometriosis or adenomyosis of the rectovaginal septum. This lesion originates from the rectovaginal septum tissue and consists essentially of smooth muscle with active glandular epithelium and scanty stroma. In our study, the rectovaginal nodule was histologically similar to an adenomyoma (Zaloudek and Norris, 1987). It was a circumscribed, nodular aggregate of smooth muscle, endometrial glands and endometrial stroma. As in the 'adenomyoma', secretory changes were frequently absent in 'endometriotic' rectovaginal nodules. The invasion of the muscle by a very active glandular epithelium, without stroma, proved that the stroma is not necessary for invasion in this particular type of pathology called adenomyosis. In some instances, it can be seen that the vaginal pluristratified epithelium was replaced by a glandular epithelium. The fact that ciliated cells were present and the co-expression of both vimentin and cytokeratin (Donnez and Nisolle, personal communication) proved the Mullerian origin of the nodule, where certain histological characteristics are completely different to those observed in peritoneal lesions (Nisolle et al, 1990). In our series, deep fibrotic tissue assumed to contain endometriosis was excised or vaporized from the anterior rectum with the aid of multiple rectovaginal examinations. Cul-de-sac dissection was followed by excision of deep fibrotic endometriosis, without cul-de-sac reconstruction. In three cases, the bowel lumen was entered. A comprehensive laparoscopic procedure, while not eradicating all the endometriosis, may result in considerable pain relief or a desired pregnancy. [less ▲]Detailed reference viewed: 5 (0 ULg)