Attachment of the Gammaherpesvirus Bovine Herpesvirus 4 Is Mediated by the Interaction of Gp8 Glycoprotein with Heparinlike Moieties on the Cell SurfaceVanderplasschen, Alain ; ; et alin Virology (1993), 196(1), 232-40 Cell surface heparan sulfate serves as the initial receptor for several alphaherpesviruses and at least one betaherpesvirus. This study shows that during the process of adsorption of the gammaherpesvirus ... [more ▼] Cell surface heparan sulfate serves as the initial receptor for several alphaherpesviruses and at least one betaherpesvirus. This study shows that during the process of adsorption of the gammaherpesvirus bovine herpesvirus 4 (BHV-4), the viral glycoprotein gp8 interacts with heparinlike moieties of cell surface. This conclusion is based on the following findings. (i) Soluble heparin was capable of blocking BHV-4 infection of Georgia bovine kidney cells by inhibition of viral attachment. (ii) Nevertheless, after virus adsorption to Georgia bovine kidney cells, heparin was partially capable of removing adsorbed virus. (iii) Enzymatic digestion of cell surface heparan sulfate but not of chondroitin sulfates A, B, and C reduced the binding of the virus to the cells, and rendered the cells partially resistant to infection. (iv) Radiolabeled purified BHV-4 bound to wild-type Chinese hamster ovary cells, whereas binding of the virus to mutant Chinese hamster ovary cell lines that where deficient in either all glycosaminoglycans or only heparan sulfate was significantly impaired. (v) Using heparin-affinity chromatography, gp8 glycoprotein was shown to bind specifically to immobilized heparin and to elute in the presence of soluble heparin. These data together showed that the gammaherpesvirus BHV-4, like alphaherpesviruses and one betaherpesvirus, adsorbs to cells by binding to cell surface heparin-like moieties. Therefore, this study extends the group of herpesviruses interacting with heparinlike moieties at the cell surface to a member of the gammaherpesvirinae subfamily. [less ▲] Detailed reference viewed: 13 (1 ULg) Stability of a FBTCS Scheme Applied to the Propagation of Shallow-Water Inertia-Gravity Waves on Various Space GridsBeckers, Jean-Marie ; in Journal of Computational Physics (1993), 108(1), 94-104 The equations governing the propagation of inertia-gravity waves in geophysical fluid flows are discretized on the A, B, C, and D grids according to the classical forward-backward on time and centered on ... [more ▼] The equations governing the propagation of inertia-gravity waves in geophysical fluid flows are discretized on the A, B, C, and D grids according to the classical forward-backward on time and centered on space (FBTCS) numerical scheme. The von Neumann stability analysis is performed and it is shown that the stability condition of the inertia-gravity waves scheme is more restrictive, at least by a factor of , than that concerning pure gravity waves, whatever the magnitude of the Coriolis parameter. Finally, the general necessary and sufficient stability condition is derived for the A, B and C grids, while, on the D grid, the stability condition has been obtained only in particular cases. [less ▲] Detailed reference viewed: 33 (12 ULg) BPDZ 44 : a new and potent activator of ATP-sensitive K+ channels; Pirotte, Bernard ; et alPoster (1993, September) Detailed reference viewed: 2 (0 ULg) Comment rendre LOTOS apte à spécifier des systèmes temps réel ?Leduc, Guy ; in Dssouli, Rachida; Bochmann, Gregor (Eds.) Ingénierie des Protocoles (1993, September) We explain and justify by examples our proposed timed extension of LOTOS. The examples cover protocol functions and service facilities that are frequently encountered in practice or are inspired by ... [more ▼] We explain and justify by examples our proposed timed extension of LOTOS. The examples cover protocol functions and service facilities that are frequently encountered in practice or are inspired by existing ones, such as timers, watchdogs, delays, isochronism, multimedia, rate control, ... The semantics of the language is presented after, and its properties discussed more formally. We think that the systematic application of formal temporal techniques to this kind of examples is unavoidable to convince of the pertinence of the design choices. The practical effectiveness of a formalism is as important as the associated mathematical properties. [less ▲] Detailed reference viewed: 14 (1 ULg) Technological aspects of biopesticides production. Dans "Biological control of fruit and foliar diseases" Proceedings of an EC workshop, Gembloux, September 16-17.Thonart, Philippe ; ; et alin Proceedings "Biological control of fruit and foliar diseases" (1993, September) Detailed reference viewed: 11 (2 ULg) In vitro antagonism of two potential biopesticides Bacillus subtilis and Pseudomonas fluorescens : biochemical aspects.; ; Ongena, Marc et alin Proceedings "Biological control of fruit and foliar diseases" (1993, September) Detailed reference viewed: 15 (3 ULg) Acremonium luzulae produces an antifungal agent with a very strong activity.; ; Thonart, Philippe ![]() Poster (1993, September) Detailed reference viewed: 6 (0 ULg) No Increase in Plasma Histamine During Paf-Induced Airway Obstruction in Allergic AsthmaticsLouis, Renaud ; Bury, Thierry ; Corhay, Jean-Louis et alin CHEST (1993), 104(3), 806-10 To investigate the possible role of mast cell or basophil histamine release in mediating platelet-activating factor (PAF) airway obstruction, we studied the effect of inhaled PAF (30 micrograms, single ... [more ▼] To investigate the possible role of mast cell or basophil histamine release in mediating platelet-activating factor (PAF) airway obstruction, we studied the effect of inhaled PAF (30 micrograms, single dose) on plasma histamine, bronchial caliber, and leukocyte and platelet counts in six patients with mild or moderate allergic asthma (mean age, 27 +/- 1.3 years; mean FEV1, 95 +/- 5 percent of predicted; mean PC20 methacholine, 1.46 +/- 0.36 mg/ml). Specific conductance (SGaw) FEV1, FEF25-75 percent, differential leukocyte and platelet counts, and plasma histamine (radioimmunoassay) were measured before and 5, 10, 15, and 20 min after PAF inhalation. Mean basal plasma histamine level was 0.28 +/- 0.04 ng/ml. Inhalation of PAF caused a fall in SGaw peaking at 5 min (43 +/- 9 percent) and a fall in FEV1 and FEF25-75 peaking at 10 min (19 +/- 10 percent and 30 +/- 13 percent, respectively). There was also a rapid and transient fall in circulating neutrophils at 5 min (from 3,096 +/- 204/mm3 to 2,551 +/- 158/mm3, p < 0.05) followed by a rebound neutrophilia. In contrast, plasma histamine level did not change significantly at any time measured. Conversely in the same asthmatics, a rapid rise in plasma histamine level (from 0.29 +/- 0.03 ng/ml at baseline to 0.53 +/- 0.06 ng/ml at 5 min; p < 0.01) was observed after an allergenic challenge (Dermatophagoides pteronyssinus) causing a fall in FEV1 peaking at 10 min (22 +/- 4 percent). Thus, inhaled PAF may induce airway obstruction and neutropenia in asthmatics without any significant change of plasma histamine level. These results indicate that it is unlikely that lung mast cells or basophils degranulate during PAF-induced bronchoconstriction. [less ▲] Detailed reference viewed: 6 (1 ULg) Acute Biochemical Variations Induced by Four Different Calcium Salts in Healthy Male VolunteersReginster, Jean-Yves ; ; Bartsch, Valérie et alin Osteoporosis International : A Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis & the National Osteoporosis Foundation of the USA (1993), 3 Calcium (Ca) supplements have positive effects in growing children, reduce bone loss in late-postmenopausal women with a low calcium diet and, in association with vitamin D3 supplements, may reduce non ... [more ▼] Calcium (Ca) supplements have positive effects in growing children, reduce bone loss in late-postmenopausal women with a low calcium diet and, in association with vitamin D3 supplements, may reduce non-vertebral fracture rates in elderly women. However, for many formulated pharmaceutical products their relative beneficial effects have not been conclusively established. We have compared the acute (6 h) metabolic responses following oral administration of two preparations of calcium gluconolactate and carbonate (CG and CG'), tricalcium phosphate (TCP) and calcium citrate (CC), given on separate occasions in each of 10 healthy young male volunteers. The subjects fasted overnight for 12 h and continued to fast during the experimental procedure. A 1000 mg dose of each Ca salt was ingested at weekly intervals. Blood was drawn after 30, 60, 90, 120, 180, 240, 300 and 360 min for measurement of serum Ca, phosphorus (P), parathyroid hormone (PTH) and whole plasma calcitonin (iCT). All Ca supplements induced significant (+6.4% to +8.1%; p < 0.01) increases in Ca and significant suppression of PTH (-37.4% to -57.4%; p < 0.01). Comparison of response curves revealed significantly (p < 0.01) more marked Ca increase and PTH suppression with CC than with the other three Ca salts. CG' and CC induced marginal decreases in serum P and the overall curve of P variations was different for TCP compared with CG, CG' and CC. No significant variation of iCT was recorded during the test.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲] Detailed reference viewed: 6 (3 ULg) Sous le swing de la croixSacré, Robert ![]() in Soul Bag (1993), (132), 12-16 Des Hymnes aux Negro Spirituals et aux Gospel Songs Detailed reference viewed: 1 (0 ULg) Prevention of Osteoporosis with Nasal Salmon Calcitonin: Effect of Anti-Salmon Calcitonin Antibody FormationReginster, Jean-Yves ; ; Deroisy, Rita et alin Osteoporosis International : A Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis & the National Osteoporosis Foundation of the USA (1993), 3 The amino acid sequence of salmon calcitonin (SCT) differs considerably from that of the human hormone and specific antibodies (Ab) develop in a significant proportion of patients after parenteral or ... [more ▼] The amino acid sequence of salmon calcitonin (SCT) differs considerably from that of the human hormone and specific antibodies (Ab) develop in a significant proportion of patients after parenteral or nasal administration of SCT. Controversy remains regarding the functional importance of these Ab. We report on the development of specific anti-SCT Ab in a population of postmenopausal women receiving nasal SCT for prevention of postmenopausal bone loss, and compare the effects of nasal SCT in women with or without Ab. Thirty-nine per cent of women developed Ab after 6 months of treatment with SCT, 52% after 12 months, and 61% after 18 and 24 months. After 24 months the AB titre was 3.47-17.7 x 10(-9) M/l (mean +/- SD: 13.3 +/- 3.1 x 10(-9) M/l). No significant differences appeared between the changes in lumbar bone mineral density (BMD) measured in the whole population (n = 44) (mean +/- SD: +1.06 +/- 3.9%), the patients without Ab (n = 17) (+0.05 +/- 3.7%) or in those with Ab (n = 27) (+1.7 +/- 4.6%). During the same period, a control population randomly assigned to a 500 mg/day calcium intake showed a significant loss of lumbar BMD (-4.57 +/- 4.9%) (p < 0.01). In conclusion, in healthy postmenopausal women nasal SCT seems to maintain the same preventive effect against bone loss whether or not Ab are present. [less ▲] Detailed reference viewed: 8 (3 ULg) La nature des représentations visuelles en tant que fonction de la profondeur d’encodageCollette, Fabienne ![]() Master's dissertation (1993) Detailed reference viewed: 4 (0 ULg) Contribution à l'étude de la phase précoce du choc septique :Analyse des effets hémodynamiques provoqués par l'administration continue de faibles doses d'endotoxine chez le chien.D'ORIO, Vincenzo ![]() Doctoral thesis (1993) Modèle expérimental visant à démontrer que la perfusion soutenue d'une faible dose d'endotoxine reproduit le tableau clinique constaté chez l'homme. Detailed reference viewed: 6 (2 ULg) Analysis of emergency voltage situationsVan Cutsem, Thierry ![]() in Proc. 11th Power System Computation Conference (1993, September) Detailed reference viewed: 17 (1 ULg) An approach to corrective control of voltage stability using simulation and sensitivityVan Cutsem, Thierry ![]() in Proc. IEEE NTUA Athens Power Tech conference (1993, September) Detailed reference viewed: 15 (0 ULg) Terpenoids production by normal and transformed cultures of Artemisia annua L.; ; et al Poster (1993, August 31) Detailed reference viewed: 9 (2 ULg) "Fan coaching activities in football" / Recherche-action sur la violence dans les stades de footballComeron, Manuel ![]() Scientific conference (1993, August 23) Socio-political change and crime - A challenge of the 21st century Detailed reference viewed: 14 (1 ULg) Macromolecular engineering of polylactones and polylactides. 12. Study of the depolymerizatin reactions of poly(ε-caprolactone with functional aluminum alkoxide end groups; ; Jérôme, Robert et alin Macromolecules (1993), 26(17), 4407-4412 Ring-opening polymerization of epsilon-caprolactone has been initiated with functional aluminum alkoxides in toluene at 25-degrees-C. Degradation of ''living'' polyester chains by transesterification ... [more ▼] Ring-opening polymerization of epsilon-caprolactone has been initiated with functional aluminum alkoxides in toluene at 25-degrees-C. Degradation of ''living'' polyester chains by transesterification reactions has been studied in relation to temperature, solvent polarity, average molecular weight, and structure of the initiator, i.e., the nature of the functional group and the number of alkoxides per Al atom. Degradation products, particularly cyclic oligomers, have been isolated by supercritical fluid extraction (SFE) and characterized by GC-MS. The effect of Lewis bases on transesterification reactions has been investigated and discussed in reference to the ''coordination-insertion'' mechanism. [less ▲] Detailed reference viewed: 4 (1 ULg) Treatment of TSH-secreting pituitary adenomas with octreotide : A floow-up of 52 patients; ; et al in Annals of Internal Medicine (1993), 119(3), 236-240 Detailed reference viewed: 4 (0 ULg) Interactions between Active-Site-Serine Beta-Lactamases and Compounds Bearing a Methoxy Side Chain on the Alpha-Face of the Beta-Lactam Ring: Kinetic and Molecular Modelling StudiesMatagne, André ; ; Dive, Georges et alin Biochemical Journal (1993), 293((Pt 3)), 607-611 The interactions between three class A beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring (cefoxitin, moxalactam and temocillin) have been studied. When ... [more ▼] The interactions between three class A beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring (cefoxitin, moxalactam and temocillin) have been studied. When compared with the situation prevailing with good substrates, both acylation and deacylation steps appeared to be severely impaired. Molecular modelling studies of the structures of the Henri-Michaelis complexes and of the acyl-enzymes indicate a major displacement of the crystallographically observed water molecule which connects the glutamate-166 and serine-70 side chains and underline the role of this water molecule in both reaction steps. [less ▲] Detailed reference viewed: 8 (4 ULg) |
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